MD10G1266200.v1.1

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002718
Physical Location & Seq
Reverse (-)
35916263 .. 35917897
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1266200.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGACCCACACCCCACTCCACATTTACGTTAAATTACATGGCCAAAGTTTCTTCATCCCTGGCATGGGAGAACCTCAAGAAGTGCAACTCAACATTGTAGTTAATGACTCAAATGAAGCAAACTCTTCAGGGTGCACAGATGCTCGCGAACAAGCACTGCTTCCCCGGCGTAGGAGCTTCAACCGGTGGCTTAGGATGGCCATTTGTACGGTCTTTGTCATCGCCGGCCAATCAGCTGCAACGCTGTTGGCAAGATTATACTACGACAAAGGTGGCAAAAGCAAATGGCTGGGAGCGCTAGTACAACTTGCTGGCTTCCCTATCCTCCTTCCCTATTATTTCTTCCCAACAAAAAGAACAAATCCAACCACAAACATTAGTACCAGTACTACTCCCGAATCAAAAGAACCCTCGACTTTAATCCTTGCATCAATTTATGTCTCTCTCGGCCTTCTAATAGCCTTGGGCTGCTTCTTGTACTCGGTTGGATTATCTTACCTTCCCGTCTCTACTTATTCCCTCATTTGTGCATCCCAATTGGCATTCAATGCGCTATTCTCCTTCTTCCTCAACTCGCAAAAGTTCACCCTTTATATTGTCAATGCCCTAGTCCTCCTCACCATCTCCTCAACCCTCCTCGTGCTCCATGGTGACTCAACAGACGATCCAAGTGGAGGATCAAAAGCAAAGTATGCGATCGGGTTCATATGTACCCTTGGCGCATCAGCTGAATACTCATTAACGCTTTCTCTATCGCAGCTCGTCTTCAGCAAAGTTATAAGGAGGGAAACATTTAGAGTGGTCATGGATATGATAGTGTATCAATCTCTTGTTGCTACCTGCGCCATCTTGGTGGGACTTTTCGGTAGCGGAGAGTGGAAGGGTTTGAGGAACGAGATGGAGGGGTACGAATTAGGGAAGGTATCCTATGTCATGAACTTGATATGGTCAGCTATAATATGGCAACTCTTTGATGTTGGCATGGTGGGATTGGTTTTTGAGGCATCTTCTCTCTTCTCCAATGCCATTAATGTTGTGGGTTTGCCTGTTGTTCCAGTTGTAGCTGTGATTTTCTTCCATGACAAAATGGACGGGATTAAGGCAATGGCCTTGGTTTTGGCCATCTGGGGATTTGTTTCATACGTTTACCAGCACTACCTTGATGATCAAAAGTCCAAGATCGAGAACAAAAATGGAAATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

402

Amino Acids

44.14

Weight (kDa)

8.4

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 66 - 391 5.5e-126 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 779
Acc36I ACCTGC 1 cut(s) 848
AccII CGCG 1 cut(s) 147
AciI CCGC 1 cut(s) 870
AclWI GGATC 2 cut(s) 659, 685
AcoI YGGCCR 4 cut(s) 40, 198, 226, 1119
AcuI CTGAAG 2 cut(s) 111, 751
AfaI GTAC 7 cut(s) 208, 303, 382, 388, 479, 712, 908
AfeI AGCGCT 1 cut(s) 297
AfiI CCNNNNNNNGG 4 cut(s) 64, 65, 171, 674
AgeI ACCGGT 1 cut(s) 183
AgsI TTSAA 2 cut(s) 181, 547
AjnI CCWGG 1 cut(s) 58
AluBI AGCT 6 cut(s) 177, 236, 728, 760, 953, 1064
AluI AGCT 6 cut(s) 177, 236, 728, 760, 953, 1064
Alw21I GWGCWC 2 cut(s) 137, 645
Alw26I GTCTC 2 cut(s) 445, 511
Alw44I GTGCAC 1 cut(s) 133
AlwI GGATC 2 cut(s) 659, 685
Aor51HI AGCGCT 1 cut(s) 297
AoxI GGCC 6 cut(s) 40, 198, 226, 448, 1107, 1119
ApaLI GTGCAC 1 cut(s) 133
ApeKI GCWGC 3 cut(s) 236, 468, 757
AseI ATTAAT 1 cut(s) 1029
AsiGI ACCGGT 1 cut(s) 183
AspLEI GCGC 4 cut(s) 298, 553, 722, 845
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 3 cut(s) 577, 610, 662
BaeGI GKGCMC 1 cut(s) 137
BaeI ACNNNNGTAYC 2 cut(s) 364, 397
BalI TGGCCA 3 cut(s) 42, 200, 1121
BauI CACGAG 1 cut(s) 638
BbsI GAAGAC 1 cut(s) 757
Bbv12I GWGCWC 2 cut(s) 137, 645
BbvI GCAGC 3 cut(s) 223, 455, 769
BccI CCATC 5 cut(s) 190, 629, 854, 892, 1130
BciT130I CCWGG 1 cut(s) 60
BciVI GTATCC 1 cut(s) 934
BclI TGATCA 1 cut(s) 1165
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 445, 511
BfaI CTAG 2 cut(s) 299, 608
BfoI RGCGCY 1 cut(s) 299
BfuAI ACCTGC 1 cut(s) 848
BfuI GTATCC 1 cut(s) 934
BisI GCNGC 3 cut(s) 237, 469, 758
BlsI GCNGC 3 cut(s) 238, 470, 759
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 60, 166
BmrFI CCNGG 2 cut(s) 60, 166
BmsI GCATC 5 cut(s) 130, 437, 539, 731, 1013
BpiI GAAGAC 1 cut(s) 757
Bpu10I CCTNAGC 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 166
BsaJI CCNNGG 6 cut(s) 58, 164, 462, 646, 715, 1110
BsaWI WCCGGW 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 65, 171, 674
Bse118I RCCGGY 2 cut(s) 183, 224
Bse1I ACTGG 2 cut(s) 384, 1055
Bse3DI GCAATG 1 cut(s) 1110
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 6 cut(s) 58, 164, 462, 646, 715, 1110
BseGI GGATG 3 cut(s) 54, 201, 530
BseLI CCNNNNNNNGG 4 cut(s) 64, 65, 171, 674
BseMI GCAATG 1 cut(s) 1110
BseNI ACTGG 2 cut(s) 384, 1055
BseRI GAGGAG 3 cut(s) 605, 616, 626
BseSI GKGCMC 1 cut(s) 137
BseXI GCAGC 3 cut(s) 223, 455, 769
BseYI CCCAGC 1 cut(s) 289
Bsh1236I CGCG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 6 cut(s) 42, 200, 228, 450, 1109, 1121
BshTI ACCGGT 1 cut(s) 183
BsiEI CGRYCG 1 cut(s) 699
BsiHKAI GWGCWC 2 cut(s) 137, 645
BsiSI CCGG 3 cut(s) 166, 184, 225
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 4 cut(s) 64, 65, 171, 674
BsmAI GTCTC 2 cut(s) 445, 511
BsmBI CGTCTC 1 cut(s) 511
BsmFI GGGAC 1 cut(s) 870
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 6 cut(s) 42, 200, 228, 450, 1109, 1121
Bsp1286I GDGCHC 2 cut(s) 137, 645
Bsp143I GATC 5 cut(s) 664, 677, 696, 1165, 1179
Bsp19I CCATGG 1 cut(s) 646
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 1 cut(s) 870
BspANI GGCC 6 cut(s) 42, 200, 228, 450, 1109, 1121
BspFNI CGCG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 933
BspMI ACCTGC 1 cut(s) 848
BspPI GGATC 2 cut(s) 659, 685
BsrDI GCAATG 1 cut(s) 1110
BsrFI RCCGGY 2 cut(s) 183, 224
BsrI ACTGG 2 cut(s) 384, 1055
BssAI RCCGGY 2 cut(s) 183, 224
BssECI CCNNGG 6 cut(s) 58, 164, 462, 646, 715, 1110
BssMI GATC 5 cut(s) 664, 677, 696, 1165, 1179
BssSI CACGAG 1 cut(s) 638
BssT1I CCWWGG 4 cut(s) 462, 646, 715, 1110
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 2 cut(s) 130, 1019
BstAPI GCANNNNNTGC 2 cut(s) 548, 692
BstC8I GCNNGC 3 cut(s) 145, 226, 313
BstDEI CTNAG 1 cut(s) 191
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 3 cut(s) 54, 201, 530
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 4 cut(s) 298, 553, 722, 845
BstKTI GATC 5 cut(s) 667, 680, 699, 1168, 1182
BstMAI GTCTC 2 cut(s) 445, 511
BstMBI GATC 5 cut(s) 664, 677, 696, 1165, 1179
BstMCI CGRYCG 1 cut(s) 699
BstMWI GCNNNNNNNGC 5 cut(s) 166, 295, 548, 692, 842
BstNI CCWGG 1 cut(s) 60
BstSCI CCNGG 2 cut(s) 58, 164
BstSLI GKGCMC 1 cut(s) 137
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 223, 455, 769
BstV2I GAAGAC 1 cut(s) 757
BstXI CCANNNNNNTGG 1 cut(s) 853
BsuI GTATCC 1 cut(s) 934
BsuRI GGCC 6 cut(s) 42, 200, 228, 450, 1109, 1121
BtgI CCRYGG 1 cut(s) 646
BtgZI GCGATG 1 cut(s) 205
BtsCI GGATG 3 cut(s) 54, 201, 530
BtsI GCAGTG 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 155
BtuMI TCGCGA 1 cut(s) 147
BveI ACCTGC 1 cut(s) 848
Cac8I GCNNGC 3 cut(s) 145, 226, 313
CciI TCATGA 1 cut(s) 933
CfoI GCGC 4 cut(s) 298, 553, 722, 845
Cfr10I RCCGGY 2 cut(s) 183, 224
Csp6I GTAC 7 cut(s) 207, 302, 381, 387, 478, 711, 907
CspAI ACCGGT 1 cut(s) 183
CviAII CATG 7 cut(s) 38, 64, 647, 805, 934, 982, 1079
CviQI GTAC 7 cut(s) 207, 302, 381, 387, 478, 711, 907
DdeI CTNAG 1 cut(s) 191
DpnI GATC 5 cut(s) 666, 679, 698, 1167, 1181
DpnII GATC 5 cut(s) 664, 677, 696, 1165, 1179
EaeI YGGCCR 4 cut(s) 40, 198, 226, 1119
Eam1104I CTCTTC 2 cut(s) 130, 1019
EarI CTCTTC 2 cut(s) 130, 1019
Eco130I CCWWGG 4 cut(s) 462, 646, 715, 1110
Eco47III AGCGCT 1 cut(s) 297
Eco57I CTGAAG 2 cut(s) 111, 751
EcoRII CCWGG 1 cut(s) 58
EcoT14I CCWWGG 4 cut(s) 462, 646, 715, 1110
ErhI CCWWGG 4 cut(s) 462, 646, 715, 1110
Esp3I CGTCTC 1 cut(s) 511
FaeI CATG 7 cut(s) 41, 67, 650, 808, 937, 985, 1082
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FaqI GGGAC 1 cut(s) 870
FatI CATG 7 cut(s) 37, 63, 646, 804, 933, 981, 1078
FauNDI CATATG 1 cut(s) 707
FbaI TGATCA 1 cut(s) 1165
Fnu4HI GCNGC 3 cut(s) 237, 469, 758
FokI GGATG 3 cut(s) 41, 208, 517
Fsp4HI GCNGC 3 cut(s) 237, 469, 758
FspBI CTAG 2 cut(s) 299, 608
GlaI GCGC 4 cut(s) 297, 552, 721, 844
GluI GCNGC 3 cut(s) 237, 469, 758
GsaI CCCAGC 1 cut(s) 293
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 6 cut(s) 42, 200, 228, 450, 1109, 1121
HapII CCGG 3 cut(s) 166, 184, 225
HhaI GCGC 4 cut(s) 298, 553, 722, 845
Hin1II CATG 7 cut(s) 41, 67, 650, 808, 937, 985, 1082
Hin6I GCGC 4 cut(s) 296, 551, 720, 843
HinP1I GCGC 4 cut(s) 296, 551, 720, 843
HinfI GANTC 3 cut(s) 107, 398, 653
HpaII CCGG 3 cut(s) 166, 184, 225
HphI GGTGA 3 cut(s) 577, 610, 662
Hpy166II GTNNAC 3 cut(s) 135, 585, 1147
Hpy188III TCNNGA 5 cut(s) 77, 146, 395, 934, 1183
Hpy8I GTNNAC 3 cut(s) 135, 585, 1147
HpyAV CCTTC 6 cut(s) 338, 461, 509, 571, 874, 913
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 2 cut(s) 27, 1143
HpyCH4V TGCA 5 cut(s) 85, 135, 239, 428, 530
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 295, 548, 692, 842
HpyF3I CTNAG 1 cut(s) 191
HpySE526I ACGT 2 cut(s) 27, 1143
Hsp92II CATG 7 cut(s) 41, 67, 650, 808, 937, 985, 1082
HspAI GCGC 4 cut(s) 296, 551, 720, 843
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 1165
Kzo9I GATC 5 cut(s) 664, 677, 696, 1165, 1179
LmnI GCTCC 3 cut(s) 174, 293, 648
Lsp1109I GCAGC 3 cut(s) 223, 455, 769
LweI GCATC 5 cut(s) 130, 437, 539, 731, 1013
MaeI CTAG 2 cut(s) 299, 608
MaeII ACGT 2 cut(s) 27, 1143
MaeIII GTNAC 1 cut(s) 650
MalI GATC 5 cut(s) 666, 679, 698, 1167, 1181
MboI GATC 5 cut(s) 664, 677, 696, 1165, 1179
MboII GAAGA 8 cut(s) 43, 117, 334, 556, 757, 999, 1006, 1066
MfeI CAATTG 1 cut(s) 536
MhlI GDGCHC 2 cut(s) 137, 645
MlsI TGGCCA 3 cut(s) 42, 200, 1121
MluCI AATT 4 cut(s) 32, 432, 536, 911
MluNI TGGCCA 3 cut(s) 42, 200, 1121
MlyI GAGTC 2 cut(s) 101, 647
MmeI TCCRAC 2 cut(s) 389, 466
Mox20I TGGCCA 3 cut(s) 42, 200, 1121
MroNI GCCGGC 1 cut(s) 224
MscI TGGCCA 3 cut(s) 42, 200, 1121
MseI TTAA 6 cut(s) 30, 102, 419, 740, 1029, 1098
MslI CAYNNNNRTG 2 cut(s) 809, 851
Msp20I TGGCCA 3 cut(s) 42, 200, 1121
MspA1I CMGCKG 2 cut(s) 236, 728
MspI CCGG 3 cut(s) 166, 184, 225
MspR9I CCNGG 2 cut(s) 60, 166
MunI CAATTG 1 cut(s) 536
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 1 cut(s) 60
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 5 cut(s) 166, 295, 548, 692, 842
NaeI GCCGGC 1 cut(s) 226
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 1 cut(s) 646
NdeI CATATG 1 cut(s) 707
NdeII GATC 5 cut(s) 664, 677, 696, 1165, 1179
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 7 cut(s) 41, 67, 650, 808, 937, 985, 1082
NmeAIII GCCGAG 1 cut(s) 426
NmuCI GTSAC 1 cut(s) 650
NruI TCGCGA 1 cut(s) 147
PagI TCATGA 1 cut(s) 933
PctI GAATGC 1 cut(s) 542
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 1 cut(s) 398
PinAI ACCGGT 1 cut(s) 183
PkrI GCNGC 3 cut(s) 238, 470, 759
Ple19I CGATCG 1 cut(s) 699
PleI GAGTC 2 cut(s) 101, 647
PpsI GAGTC 2 cut(s) 101, 647
PshBI ATTAAT 1 cut(s) 1029
PsiI TTATAA 1 cut(s) 779
Psp6I CCWGG 1 cut(s) 58
PspFI CCCAGC 1 cut(s) 289
PspGI CCWGG 1 cut(s) 58
PvuI CGATCG 1 cut(s) 699
PvuII CAGCTG 2 cut(s) 236, 728
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 7 cut(s) 208, 303, 382, 388, 479, 712, 908
RsaNI GTAC 7 cut(s) 207, 302, 381, 387, 478, 711, 907
RseI CAYNNNNRTG 2 cut(s) 809, 851
SaqAI TTAA 6 cut(s) 30, 102, 419, 740, 1029, 1098
SatI GCNGC 3 cut(s) 237, 469, 758
Sau3AI GATC 5 cut(s) 664, 677, 696, 1165, 1179
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 2 cut(s) 101, 647
ScrFI CCNGG 2 cut(s) 60, 166
SduI GDGCHC 2 cut(s) 137, 645
SfaNI GCATC 5 cut(s) 130, 437, 539, 731, 1013
SmiMI CAYNNNNRTG 2 cut(s) 809, 851
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 4 cut(s) 32, 432, 536, 911
SsiI CCGC 1 cut(s) 870
SspMI CTAG 2 cut(s) 299, 608
StyD4I CCNGG 2 cut(s) 58, 164
StyI CCWWGG 4 cut(s) 462, 646, 715, 1110
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 2 cut(s) 30, 1146
TaqI TCGA 2 cut(s) 413, 1182
TasI AATT 4 cut(s) 32, 432, 536, 911
TatI WGTACW 3 cut(s) 301, 386, 477
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 6 cut(s) 30, 102, 419, 740, 1029, 1098
Tru9I TTAA 6 cut(s) 30, 102, 419, 740, 1029, 1098
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 3 cut(s) 236, 468, 757
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 5 cut(s) 43, 129, 694, 950, 1128
TspRI CASTG 1 cut(s) 162
VneI GTGCAC 1 cut(s) 133
VspI ATTAAT 1 cut(s) 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 1033
XspI CTAG 2 cut(s) 299, 608
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.