AT4G30470

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001892
Physical Location & Seq
Forward (+)
14894073 .. 14896844
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30470.1

Sequence Viewer

Length: 912 bp
ATGGATCACGACAAGTCTACTTCTTGTTGCTGCGTTCTTGATGCTTCCACTTATGTTGGTTTCTGGATTCTCAAGAAATTGCTTAGCAGAGGATACTCTGTTCACGCAGCGATTCGTAGAAACGGGGAGAGTGAAATTGAGGAGATGATCAGAGAGATGGAAACAACAGAAGAGAGATTAGTGGTGTACGATGTAGATGTGTTGGATTATCAAAGCATACTTGTCTCTCTCAAGACATGTAACGTTGTCTTCTGTTGCTTAGACAGCCCTGAAGGGTACGATGAGAAGGAGGTGGATTTGGAAGTGAGAGGAGCGATCAATGTGGTGGAAGCGTGTGGAAGAACAGAGAGTATAGAGAAGATTGTGTTTTCTTCTTCATTAACAGCTTCAATTTGGAGAGACAACATTGGAACTCAAAAGGATGTTGATGAGAAGTGTTGGAGTGACCAAGACTTCTGTCGCAGCAAGAAGTTGTGGCATGCACTGGCAAAGATGTTGTCGGAGAAAGCAGCTTGGGCATTAGCCATGGACCGTAGGCTCAACATGGTCTCTATCAACCCTGGTCTTGTCGTCGGACCATCTGTCGCACAACACAACGCTAGGCCCACCATGTCATACCTTAAAGGAGCTGCACAAATGTATGAGAATGGTGTGTTAGCTTATGTAGACGTTAAGTTTCTAGCGGACGTTCATATTAGAGCATTCGAGGATGTTTCAGCTTGCGGTCGATACTTCTGCTTCAACCAAATCGTCAACACAGAAGAAGAAGCTCTCAAGCTAGTGGAGAGTTTGTCTCCTTTGATTCCTATGCCACCGAGGTATGAGAATGAGATGCACGGAAGTGAAGTTTACGAAGAAAGATTAAGGAACAACAAATTAAGCAAGCTGGTAGAAGCTGGCTCTGCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

303

Amino Acids

34.2

Weight (kDa)

5.04

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 15 - 217 1.8e-10 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 19 - 130 6.9e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 17, 666
AciI CCGC 2 cut(s) 683, 723
AclI AACGTT 1 cut(s) 243
AclWI GGATC 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 2 cut(s) 188, 278
AflIII ACRYGT 1 cut(s) 236
AgsI TTSAA 2 cut(s) 390, 742
AhdI GACNNNNNGTC 1 cut(s) 581
AjnI CCWGG 1 cut(s) 559
AleI CACNNNNGTG 1 cut(s) 840
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
AluBI AGCT 9 cut(s) 386, 512, 629, 659, 719, 770, 778, 886, 896
AluI AGCT 9 cut(s) 386, 512, 629, 659, 719, 770, 778, 886, 896
Alw26I GTCTC 4 cut(s) 229, 393, 553, 798
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 602
ApeKI GCWGC 5 cut(s) 30, 107, 462, 509, 629
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AspS9I GGNCC 3 cut(s) 529, 575, 603
AvaII GGWCC 2 cut(s) 529, 575
BbsI GAAGAC 1 cut(s) 241
BbvI GCAGC 5 cut(s) 17, 119, 474, 521, 616
BccI CCATC 2 cut(s) 151, 586
BcgI CGANNNNNNTGC 2 cut(s) 717, 751
BciT130I CCWGG 1 cut(s) 561
BciVI GTATCC 1 cut(s) 86
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 4 cut(s) 229, 393, 553, 798
BfaI CTAG 3 cut(s) 600, 680, 779
BfuI GTATCC 1 cut(s) 86
BisI GCNGC 5 cut(s) 31, 108, 463, 510, 630
BlpI GCTNAGC 1 cut(s) 83
BlsI GCNGC 5 cut(s) 32, 109, 464, 511, 631
Bme1390I CCNGG 1 cut(s) 561
Bme18I GGWCC 2 cut(s) 529, 575
BmeRI GACNNNNNGTC 1 cut(s) 581
BmgT120I GGNCC 3 cut(s) 529, 575, 603
BmrFI CCNGG 1 cut(s) 561
BmsI GCATC 2 cut(s) 31, 822
BoxI GACNNNNGTC 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 778, 810
Bpu1102I GCTNAGC 1 cut(s) 83
BpuEI CTTGAG 3 cut(s) 56, 215, 758
BsaI GGTCTC 1 cut(s) 553
BsaJI CCNNGG 3 cut(s) 525, 559, 815
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bse1I ACTGG 1 cut(s) 489
BseBI CCWGG 1 cut(s) 561
BseDI CCNNGG 3 cut(s) 525, 559, 815
BseGI GGATG 2 cut(s) 427, 715
BseNI ACTGG 1 cut(s) 489
BseRI GAGGAG 2 cut(s) 155, 324
BseXI GCAGC 5 cut(s) 17, 119, 474, 521, 616
BsgI GTGCAG 1 cut(s) 615
Bsh1285I CGRYCG 1 cut(s) 727
BshFI GGCC 1 cut(s) 604
BsiEI CGRYCG 1 cut(s) 727
BsmAI GTCTC 4 cut(s) 229, 393, 553, 798
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 1 cut(s) 604
Bso31I GGTCTC 1 cut(s) 553
Bsp143I GATC 3 cut(s) 4, 147, 315
Bsp1720I GCTNAGC 1 cut(s) 83
Bsp19I CCATGG 1 cut(s) 525
BspACI CCGC 2 cut(s) 683, 723
BspANI GGCC 1 cut(s) 604
BspPI GGATC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 553
BsrI ACTGG 1 cut(s) 489
BssECI CCNNGG 3 cut(s) 525, 559, 815
BssMI GATC 3 cut(s) 4, 147, 315
BssT1I CCWWGG 1 cut(s) 525
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 1 cut(s) 533
Bst6I CTCTTC 1 cut(s) 165
BstC8I GCNNGC 4 cut(s) 480, 721, 884, 898
BstDEI CTNAG 2 cut(s) 83, 259
BstDSI CCRYGG 1 cut(s) 525
BstF5I GGATG 2 cut(s) 427, 715
BstKTI GATC 3 cut(s) 7, 150, 318
BstMAI GTCTC 4 cut(s) 229, 393, 553, 798
BstMBI GATC 3 cut(s) 4, 147, 315
BstMCI CGRYCG 1 cut(s) 727
BstMWI GCNNNNNNNGC 3 cut(s) 264, 515, 902
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 2 cut(s) 240, 482
BstPAI GACNNNNGTC 1 cut(s) 456
BstSCI CCNGG 1 cut(s) 559
BstV1I GCAGC 5 cut(s) 17, 119, 474, 521, 616
BstV2I GAAGAC 1 cut(s) 241
BsuI GTATCC 1 cut(s) 86
BsuRI GGCC 1 cut(s) 604
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 2 cut(s) 427, 715
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 4 cut(s) 480, 721, 884, 898
Cfr13I GGNCC 3 cut(s) 529, 575, 603
Csp6I GTAC 2 cut(s) 187, 277
CspCI CAANNNNNGTGG 2 cut(s) 37, 72
CviAII CATG 5 cut(s) 237, 479, 526, 544, 610
CviQI GTAC 2 cut(s) 187, 277
DdeI CTNAG 2 cut(s) 83, 259
DpnI GATC 3 cut(s) 6, 149, 317
DpnII GATC 3 cut(s) 4, 147, 315
DriI GACNNNNNGTC 1 cut(s) 581
Eam1104I CTCTTC 1 cut(s) 165
Eam1105I GACNNNNNGTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 525
Eco31I GGTCTC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 529, 575
Eco57I CTGAAG 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 559
EcoT14I CCWWGG 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 5 cut(s) 240, 482, 529, 547, 613
FatI CATG 5 cut(s) 236, 478, 525, 543, 609
FbaI TGATCA 1 cut(s) 147
FblI GTMKAC 2 cut(s) 17, 666
Fnu4HI GCNGC 5 cut(s) 31, 108, 463, 510, 630
FokI GGATG 2 cut(s) 434, 722
Fsp4HI GCNGC 5 cut(s) 31, 108, 463, 510, 630
FspBI CTAG 3 cut(s) 600, 680, 779
GluI GCNGC 5 cut(s) 31, 108, 463, 510, 630
HaeIII GGCC 1 cut(s) 604
Hin1II CATG 5 cut(s) 240, 482, 529, 547, 613
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HinfI GANTC 3 cut(s) 67, 112, 802
Hpy166II GTNNAC 6 cut(s) 18, 103, 187, 667, 754, 850
Hpy188I TCNGA 3 cut(s) 152, 502, 575
Hpy188III TCNNGA 5 cut(s) 8, 38, 64, 73, 232
Hpy8I GTNNAC 6 cut(s) 18, 103, 187, 667, 754, 850
Hpy99I CGWCG 1 cut(s) 575
HpyAV CCTTC 2 cut(s) 266, 280
HpyCH4III ACNGT 1 cut(s) 533
HpyCH4IV ACGT 3 cut(s) 243, 669, 687
HpyCH4V TGCA 3 cut(s) 482, 632, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 264, 515, 902
HpyF3I CTNAG 2 cut(s) 83, 259
HpySE526I ACGT 3 cut(s) 243, 669, 687
Hsp92II CATG 5 cut(s) 240, 482, 529, 547, 613
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 3 cut(s) 4, 147, 315
LmnI GCTCC 2 cut(s) 311, 626
LpnPI CCDG 7 cut(s) 49, 282, 470, 546, 573, 872, 882
Lsp1109I GCAGC 5 cut(s) 17, 119, 474, 521, 616
LweI GCATC 2 cut(s) 31, 822
MaeI CTAG 3 cut(s) 600, 680, 779
MaeII ACGT 3 cut(s) 243, 669, 687
MaeIII GTNAC 2 cut(s) 239, 443
MalI GATC 3 cut(s) 6, 149, 317
MboI GATC 3 cut(s) 4, 147, 315
MboII GAAGA 9 cut(s) 182, 241, 351, 363, 366, 370, 773, 776, 866
MluCI AATT 4 cut(s) 77, 135, 390, 875
MmeI TCCRAC 4 cut(s) 183, 419, 480, 553
MnlI CCTC 6 cut(s) 83, 133, 283, 302, 700, 810
MseI TTAA 6 cut(s) 380, 621, 672, 863, 878, 910
MslI CAYNNNNRTG 1 cut(s) 840
MspR9I CCNGG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 3 cut(s) 264, 515, 902
NcoI CCATGG 1 cut(s) 525
NdeII GATC 3 cut(s) 4, 147, 315
NlaIII CATG 5 cut(s) 240, 482, 529, 547, 613
NmuCI GTSAC 1 cut(s) 443
NspI RCATGY 2 cut(s) 240, 482
OliI CACNNNNGTG 1 cut(s) 840
PaeI GCATGC 1 cut(s) 482
PciI ACATGT 1 cut(s) 236
PctI GAATGC 1 cut(s) 701
PfeI GAWTC 3 cut(s) 67, 112, 802
PkrI GCNGC 5 cut(s) 32, 109, 464, 511, 631
PscI ACATGT 1 cut(s) 236
PshAI GACNNNNGTC 1 cut(s) 456
Psp1406I AACGTT 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspPI GGNCC 3 cut(s) 529, 575, 603
RsaI GTAC 2 cut(s) 188, 278
RsaNI GTAC 2 cut(s) 187, 277
RseI CAYNNNNRTG 1 cut(s) 840
SaqAI TTAA 6 cut(s) 380, 621, 672, 863, 878, 910
SatI GCNGC 5 cut(s) 31, 108, 463, 510, 630
Sau3AI GATC 3 cut(s) 4, 147, 315
Sau96I GGNCC 3 cut(s) 529, 575, 603
ScrFI CCNGG 1 cut(s) 561
SfaNI GCATC 2 cut(s) 31, 822
SinI GGWCC 2 cut(s) 529, 575
SmiMI CAYNNNNRTG 1 cut(s) 840
SmlI CTYRAG 3 cut(s) 71, 230, 773
SmoI CTYRAG 3 cut(s) 71, 230, 773
SphI GCATGC 1 cut(s) 482
Sse9I AATT 4 cut(s) 77, 135, 390, 875
SsiI CCGC 2 cut(s) 683, 723
SspMI CTAG 3 cut(s) 600, 680, 779
StyD4I CCNGG 1 cut(s) 559
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 1 cut(s) 533
TaiI ACGT 3 cut(s) 246, 672, 690
TaqI TCGA 2 cut(s) 705, 727
TasI AATT 4 cut(s) 77, 135, 390, 875
TfiI GAWTC 3 cut(s) 67, 112, 802
Tru1I TTAA 6 cut(s) 380, 621, 672, 863, 878, 910
Tru9I TTAA 6 cut(s) 380, 621, 672, 863, 878, 910
TscAI CASTG 1 cut(s) 489
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 5 cut(s) 30, 107, 462, 509, 629
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 2 cut(s) 366, 680
TspGWI ACGGA 1 cut(s) 852
TspRI CASTG 1 cut(s) 489
VpaK11BI GGWCC 2 cut(s) 529, 575
XceI RCATGY 2 cut(s) 240, 482
XmiI GTMKAC 2 cut(s) 17, 666
XspI CTAG 3 cut(s) 600, 680, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.