RLG00000003059

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001892
Physical Location & Seq
Forward (+)
43798282 .. 43800455
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003059

Sequence Viewer

Length: 951 bp
ATGGCTCCTGCAGCTTCGAATCCTTTCGACTACCACCACGGCGCCACGAGCTCGACCACAGTCTGCGTAATGGATGCGTCAGGCCACCTCGGCTCGACCCTCGTCGAGCGCCTGGTTCAGAGGGGCTACACAGTCCACGCCGCGCTCCAGAGCCATGGGGAATCCAAGTTGGGTTCGGTTTCTTGTGAGAATAACAAGAGGCTGAAGATTTTCCATTTAGACCCATTTGATTACCAGAGCATAGTTAATGCTCTGAAGGGCTGTTCTGGCTTGTTCTACACTTTTGAACCTCCACAAGATCAGCCCGACTATGATGAATATATGGCAGAAGTAGAGGTGAGAGCTGCACACAACGTTCTAGAAGCATGTGCCCGAACGGAAACCATAGACAAGGTTGTGTTCACATCAGCAGTCACCGCCGTCGTTTGGAGCAGTAACAACAAATCGACGGTCGCTGCTACGCCGGAGTTGGACGAACGGCACTGGACTGACGTCAACTTCTGTCGCAAGTTCAAGCTATGGCATGCCCTATCAAAAACCCTAGCAGAGAAGACAGCGTGGGCGTTGGCAATGGATAGGAGCTTGAACATGGTGTCTGTGAATGTAGGGTTGATGATAAATCCCGACTTGTCCATCAGCAACCCGTACTTGAAGGGAGCGGCGGAGATGTATGAAGACGGCGTGCTCGTGACTGTCGATATAAACTTCTTAGTGGACGCCCATATTTGCGTCTTCGAAGACATCACAGCCTACGGTCGATACTTATGTTTCAACAAAATCATCACTCAGTCGGAAGACGCTGTTAACCTTGCCCGGAAGTTGACCCCTTCTGCCCCGTCCTCGTTGCCCCAAAGCAAGGACCAGGATATGAGAATCATCCAAGAGAGAATAAGCAACAAGAAGTTGAATAAGTTGATGGTGGGTTTCAAGAGTAAATCTCAGGTGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

317

Amino Acids

35.08

Weight (kDa)

6.06

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 19 - 141 1.1e-07 NmrA-like family
3Beta_HSD PF01073 23 - 153 5.4e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 24 - 201 3.7e-07 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 26 - 141 2.1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 41
AccBSI CCGCTC 1 cut(s) 659
AccII CGCG 1 cut(s) 143
AciI CCGC 4 cut(s) 141, 417, 659, 662
AclI AACGTT 1 cut(s) 354
AcuI CTGAAG 2 cut(s) 224, 275
AcyI GRCGYC 3 cut(s) 42, 492, 717
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 2 cut(s) 426, 856
AgsI TTSAA 7 cut(s) 287, 514, 586, 652, 772, 907, 928
AjnI CCWGG 2 cut(s) 111, 861
AluBI AGCT 5 cut(s) 14, 51, 344, 517, 582
AluI AGCT 5 cut(s) 14, 51, 344, 517, 582
Alw21I GWGCWC 2 cut(s) 53, 687
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 3 cut(s) 11, 344, 455
ArsI GACNNNNNNTTYG 2 cut(s) 435, 467
Asp700I GAANNNNTTC 2 cut(s) 23, 209
AspLEI GCGC 3 cut(s) 44, 111, 145
AspS9I GGNCC 1 cut(s) 859
AsuC2I CCSGG 1 cut(s) 814
AsuHPI GGTGA 2 cut(s) 349, 406
AsuII TTCGAA 2 cut(s) 17, 735
AvaII GGWCC 1 cut(s) 859
BaeGI GKGCMC 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 751, 784
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 53
BauI CACGAG 2 cut(s) 46, 686
BbsI GAAGAC 5 cut(s) 557, 681, 724, 744, 801
Bbv12I GWGCWC 2 cut(s) 53, 687
BbvI GCAGC 3 cut(s) 23, 331, 442
BccI CCATC 2 cut(s) 641, 910
BceAI ACGGC 4 cut(s) 55, 404, 494, 694
BciT130I CCWGG 2 cut(s) 113, 863
BcnI CCSGG 1 cut(s) 814
BfaI CTAG 2 cut(s) 359, 542
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 2 cut(s) 45, 112
BglI GCCNNNNNGGC 1 cut(s) 90
BisI GCNGC 5 cut(s) 12, 141, 345, 456, 660
BlsI GCNGC 5 cut(s) 13, 142, 346, 457, 661
Bme1390I CCNGG 3 cut(s) 113, 814, 863
Bme18I GGWCC 1 cut(s) 859
BmgT120I GGNCC 1 cut(s) 859
BmiI GGNNCC 2 cut(s) 6, 43
BmrFI CCNGG 3 cut(s) 113, 814, 863
BmsI GCATC 1 cut(s) 64
BoxI GACNNNNGTC 3 cut(s) 59, 101, 491
BpiI GAAGAC 5 cut(s) 557, 681, 724, 744, 801
BplI GAGNNNNNCTC 1 cut(s) 922
BpmI CTGGAG 1 cut(s) 131
Bpu14I TTCGAA 2 cut(s) 17, 735
BpuMI CCSGG 1 cut(s) 814
BsaHI GRCGYC 3 cut(s) 42, 492, 717
BsaJI CCNNGG 3 cut(s) 37, 88, 154
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 856
Bse1I ACTGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 576
BseBI CCWGG 2 cut(s) 113, 863
BseDI CCNNGG 3 cut(s) 37, 88, 154
BseGI GGATG 2 cut(s) 79, 876
BseLI CCNNNNNNNGG 2 cut(s) 426, 856
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 1 cut(s) 800
BseNI ACTGG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 3 cut(s) 23, 331, 442
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 2 cut(s) 453, 757
BshFI GGCC 1 cut(s) 84
BshNI GGYRCC 1 cut(s) 41
BsiEI CGRYCG 2 cut(s) 453, 757
BsiHKAI GWGCWC 2 cut(s) 53, 687
BsiSI CCGG 2 cut(s) 464, 814
BslI CCNNNNNNNGG 2 cut(s) 426, 856
BsnI GGCC 1 cut(s) 84
Bsp119I TTCGAA 2 cut(s) 17, 735
Bsp1286I GDGCHC 3 cut(s) 53, 373, 687
Bsp143I GATC 1 cut(s) 298
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 4 cut(s) 141, 417, 659, 662
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 1 cut(s) 799
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 2 cut(s) 6, 43
BspMAI CTGCAG 1 cut(s) 13
BspT104I TTCGAA 2 cut(s) 17, 735
BspT107I GGYRCC 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 659
BsrDI GCAATG 1 cut(s) 576
BsrI ACTGG 1 cut(s) 488
BssECI CCNNGG 3 cut(s) 37, 88, 154
BssMI GATC 1 cut(s) 298
BssNI GRCGYC 3 cut(s) 42, 492, 717
BssSI CACGAG 2 cut(s) 46, 686
BssT1I CCWWGG 1 cut(s) 154
Bst2BI CACGAG 2 cut(s) 46, 686
Bst2UI CCWGG 2 cut(s) 113, 863
Bst4CI ACNGT 5 cut(s) 61, 133, 451, 694, 755
BstACI GRCGYC 3 cut(s) 42, 492, 717
BstBI TTCGAA 2 cut(s) 17, 735
BstC8I GCNNGC 2 cut(s) 525, 683
BstDEI CTNAG 3 cut(s) 709, 786, 939
BstDSI CCRYGG 2 cut(s) 37, 154
BstF5I GGATG 2 cut(s) 79, 876
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 2 cut(s) 45, 112
BstHHI GCGC 3 cut(s) 44, 111, 145
BstKTI GATC 1 cut(s) 301
BstMBI GATC 1 cut(s) 298
BstMCI CGRYCG 2 cut(s) 453, 757
BstMWI GCNNNNNNNGC 5 cut(s) 11, 48, 90, 267, 416
BstNI CCWGG 2 cut(s) 113, 863
BstNSI RCATGY 2 cut(s) 369, 527
BstPAI GACNNNNGTC 3 cut(s) 59, 101, 491
BstSCI CCNGG 3 cut(s) 111, 812, 861
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 3 cut(s) 23, 331, 442
BstV2I GAAGAC 5 cut(s) 557, 681, 724, 744, 801
BstXI CCANNNNNNTGG 1 cut(s) 155
BsuRI GGCC 1 cut(s) 84
BtgI CCRYGG 2 cut(s) 37, 154
BtsCI GGATG 2 cut(s) 79, 876
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 2 cut(s) 525, 683
CfoI GCGC 3 cut(s) 44, 111, 145
Cfr13I GGNCC 1 cut(s) 859
CseI GACGC 4 cut(s) 66, 718, 725, 806
Csp6I GTAC 1 cut(s) 646
CviAII CATG 4 cut(s) 155, 366, 524, 589
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 3 cut(s) 709, 786, 939
DinI GGCGCC 1 cut(s) 43
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
EciI GGCGGA 1 cut(s) 677
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 859
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 224, 275
EcoICRI GAGCTC 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 111, 861
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 4 cut(s) 158, 369, 527, 592
FatI CATG 4 cut(s) 154, 365, 523, 588
Fnu4HI GCNGC 5 cut(s) 12, 141, 345, 456, 660
FokI GGATG 2 cut(s) 86, 863
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 5 cut(s) 12, 141, 345, 456, 660
FspBI CTAG 2 cut(s) 359, 542
GlaI GCGC 3 cut(s) 43, 110, 144
GluI GCNGC 5 cut(s) 12, 141, 345, 456, 660
GsuI CTGGAG 1 cut(s) 131
HaeII RGCGCY 2 cut(s) 45, 112
HaeIII GGCC 1 cut(s) 84
HapII CCGG 2 cut(s) 464, 814
HgaI GACGC 4 cut(s) 66, 718, 725, 806
HhaI GCGC 3 cut(s) 44, 111, 145
Hin1I GRCGYC 3 cut(s) 42, 492, 717
Hin1II CATG 4 cut(s) 158, 369, 527, 592
Hin6I GCGC 3 cut(s) 42, 109, 143
HinP1I GCGC 3 cut(s) 42, 109, 143
HincII GTYRAC 3 cut(s) 496, 805, 822
HindII GTYRAC 3 cut(s) 496, 805, 822
HinfI GANTC 3 cut(s) 19, 161, 873
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 2 cut(s) 464, 814
HphI GGTGA 2 cut(s) 349, 406
Hpy166II GTNNAC 6 cut(s) 136, 402, 496, 715, 805, 822
Hpy188I TCNGA 3 cut(s) 120, 255, 793
Hpy188III TCNNGA 5 cut(s) 148, 359, 623, 688, 928
Hpy8I GTNNAC 6 cut(s) 136, 402, 496, 715, 805, 822
Hpy99I CGWCG 3 cut(s) 107, 425, 451
HpyAV CCTTC 3 cut(s) 250, 646, 837
HpyCH4III ACNGT 5 cut(s) 61, 133, 451, 694, 755
HpyCH4IV ACGT 2 cut(s) 354, 492
HpyCH4V TGCA 2 cut(s) 11, 347
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 48, 90, 267, 416
HpyF3I CTNAG 3 cut(s) 709, 786, 939
HpySE526I ACGT 2 cut(s) 354, 492
Hsp92I GRCGYC 3 cut(s) 42, 492, 717
Hsp92II CATG 4 cut(s) 158, 369, 527, 592
HspAI GCGC 3 cut(s) 42, 109, 143
KasI GGCGCC 1 cut(s) 41
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 1 cut(s) 298
LmnI GCTCC 5 cut(s) 10, 150, 429, 579, 656
Lsp1109I GCAGC 3 cut(s) 23, 331, 442
LweI GCATC 1 cut(s) 64
MaeI CTAG 2 cut(s) 359, 542
MaeII ACGT 2 cut(s) 354, 492
MaeIII GTNAC 3 cut(s) 412, 434, 688
MalI GATC 1 cut(s) 300
MbiI CCGCTC 1 cut(s) 659
MboI GATC 1 cut(s) 298
MboII GAAGA 6 cut(s) 217, 562, 686, 724, 749, 806
MhlI GDGCHC 3 cut(s) 53, 373, 687
Mly113I GGCGCC 1 cut(s) 42
MmeI TCCRAC 2 cut(s) 450, 771
MnlI CCTC 7 cut(s) 98, 110, 114, 192, 300, 328, 850
MroXI GAANNNNTTC 2 cut(s) 23, 209
MseI TTAA 2 cut(s) 246, 804
MspI CCGG 2 cut(s) 464, 814
MspR9I CCNGG 3 cut(s) 113, 814, 863
MvaI CCWGG 2 cut(s) 113, 863
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 5 cut(s) 11, 48, 90, 267, 416
NarI GGCGCC 1 cut(s) 42
NciI CCSGG 1 cut(s) 814
NcoI CCATGG 1 cut(s) 154
NdeII GATC 1 cut(s) 298
NlaIII CATG 4 cut(s) 158, 369, 527, 592
NlaIV GGNNCC 2 cut(s) 6, 43
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 412, 688
NspI RCATGY 2 cut(s) 369, 527
NspV TTCGAA 2 cut(s) 17, 735
PaeI GCATGC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 2 cut(s) 684, 693
PdmI GAANNNNTTC 2 cut(s) 23, 209
PfeI GAWTC 3 cut(s) 19, 161, 873
PkrI GCNGC 5 cut(s) 13, 142, 346, 457, 661
PluTI GGCGCC 1 cut(s) 45
PshAI GACNNNNGTC 3 cut(s) 59, 101, 491
Psp124BI GAGCTC 1 cut(s) 53
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 111, 861
PspGI CCWGG 2 cut(s) 111, 861
PspN4I GGNNCC 2 cut(s) 6, 43
PspPI GGNCC 1 cut(s) 859
PstI CTGCAG 1 cut(s) 13
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
SacI GAGCTC 1 cut(s) 53
SaqAI TTAA 2 cut(s) 246, 804
SatI GCNGC 5 cut(s) 12, 141, 345, 456, 660
Sau3AI GATC 1 cut(s) 298
Sau96I GGNCC 1 cut(s) 859
ScrFI CCNGG 3 cut(s) 113, 814, 863
SduI GDGCHC 3 cut(s) 53, 373, 687
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 1 cut(s) 9
SfoI GGCGCC 1 cut(s) 43
SfuI TTCGAA 2 cut(s) 17, 735
SinI GGWCC 1 cut(s) 859
SphI GCATGC 1 cut(s) 527
SsiI CCGC 4 cut(s) 141, 417, 659, 662
SspDI GGCGCC 1 cut(s) 41
SspMI CTAG 2 cut(s) 359, 542
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 3 cut(s) 111, 812, 861
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 5 cut(s) 61, 133, 451, 694, 755
TaiI ACGT 2 cut(s) 357, 495
TaqI TCGA 9 cut(s) 17, 27, 53, 95, 105, 446, 696, 735, 757
TauI GCSGC 2 cut(s) 143, 662
TfiI GAWTC 3 cut(s) 19, 161, 873
Tru1I TTAA 2 cut(s) 246, 804
Tru9I TTAA 2 cut(s) 246, 804
TscAI CASTG 1 cut(s) 488
TseFI GTSAC 2 cut(s) 412, 688
TseI GCWGC 3 cut(s) 11, 344, 455
Tsp45I GTSAC 2 cut(s) 412, 688
TspDTI ATGAA 2 cut(s) 330, 687
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 859
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 2 cut(s) 369, 527
XmnI GAANNNNTTC 2 cut(s) 23, 209
XspI CTAG 2 cut(s) 359, 542
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.