Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001892

Physical Location & Seq

Genomic Coordinates Forward (+)

32449565 .. 32451757

Gene Structure 2193 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1237600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGGCTCCTGCAGCAGAAGCTATTCGTAGTTTTGATGATGATGATGCTGCTGATGAAACTAGTCCATGTTCAAAGGTGACCACAGTCTGCGTCATGGATGCGTCCGGCCGCCTAGGCTCTACTCTTGTCCACCGCCTCTTGCACAGAGGCTATGCAGTCCATGCTGCACTCCAAAAGCATCACAAGTGTTTTGATGACGAATTGGTAAAGAAGAAGAAATTGAGGGTATTCCGTTCAGACCCCTTTGACTACCACAGCATTGTGGACGCCTTGAAAGGATGCTCTGCCCTTTTCTACTCCTTCGAGCCTCCTTCAGACCACCCCACTTATGATGAATTTATGGCAGAGGCAGAGGTGAGAGCAGCTCATAATGTGCTGGAAGCTTGTGCCCAAACTGATACCATACACAAAGTAGTCTTTACATCCTCTGTAACAGCTGTCATTTGGAGGGACGACCGTAATGCATCCTCGTCGGCGTCCTCCTCGTCCCGTGGCAGTGACGAGAGAAACTGGAGCGATGTCAATTTTTGTAGAAAATTCAAGCTCTGGCACGCCCTCTCCAAGACCCTGGCCGAGAGGACAGCGTGGGCTCTAGCAATGGACCGAGGACTGAACATGGTGTCCATCAATGGGGGGCTGTTGCTAGGCCCCGATCTCACCATCACGGACCCTTATCTGAAAGGAACGGCTGAGATGTACCAAGACGGGCTGTTGGTGACTGTAGATCTCAAGTTCATTGTGGACGCGCACATCTCCGTCTTTGAAGACGTGTCATCCTACGGAAGATACGTATGCTTCGACAGAGTCATCAATTGCCACAGAGATGCCGTTGAGCTTGCCCGCATGCTGTTGCCACCTTCTCAAACTTCATCTCTCCAAACGCAAAGCCAAAGCCCAAGCCAAAGCCAAAGTTTGGAGGAGGCAGAGACAATGGAGGTGTACCAACAGAAGATAAGTAACAAGAAACTCAACAAACTCATGGTCGACCTTGACACTGCCCTTCAACTCCGCGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0009058 GO:0009718 GO:0009812 GO:0009813 GO:0012505 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0019898 GO:0031090 GO:0031312 GO:0031984 GO:0042175 GO:0042406 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045552 GO:0046148 GO:0046283 GO:0055114 GO:0071704 GO:0098827 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

339

Amino Acids

37.72

Weight (kDa)

5.64

Isoelectric Point (pI)

42.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	27 - 144	8.5e-07	NmrA-like family
3Beta_HSD	PF01073	31 - 148	1.2e-07	3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase	PF01370	33 - 218	5.6e-07	NAD dependent epimerase/dehydratase family
NAD_binding_10	PF13460	34 - 146	9.5e-09	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23910 AT2G23910 AT2G23910 AT2G23910 AT4G30470
fragaria_vesca	FvH4_5g22420 FvH4_5g22420 FvH4_5g22420
malus_domestica	MD00G1072500.v1.1 MD01G1237600.v1.1 MD06G1036600.v1.1 MD07G1310200.v1.1
prunus_persica	Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.5G032800_v2.0.a1
pyrus_communis	pycom01g24530 pycom06g03000 pycom13g28670
rosa_chinensis	RchiOBHm_Chr4g0405161 RchiOBHm_Chr7g0210161
rosa_laevigata	RLG00000003059 RLG00000008867
rosa_multiflora	Rmu_sc0005438.1_g000001 Rmu_sc0006908.1_g000015 Rmu_ssc0000255.1_g000034
rosa_roxburghii	Rroxscaffold_3G00248570 Rroxscaffold_5G00349400
rosa_rugosa	Rorug04G0054400 Rorug04G0054500 Rorug04G0054600 Rorug07G0119000
rosa_samantha	Rh1AG349400 Rh4AG129400 Rh4BG123100 Rh4CG136700 Rh4DG122900 Rh7AG252500 Rh7BG247600 Rh7CG269500 Rh7DG260100
rosa_wichuraiana	Rw0G002460 Rw1G031120 Rw4G010450 Rw7G021460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	913
AccI	GTMKAC	1 cut(s)	984
AccII	CGCG	2 cut(s)	746, 1011
AciI	CCGC	4 cut(s)	109, 133, 841, 1009
AcoI	YGGCCR	2 cut(s)	106, 570
AcsI	RAATTY	2 cut(s)	335, 536
AcuI	CTGAAG	1 cut(s)	297
AcyI	GRCGYC	2 cut(s)	267, 476
AfaI	GTAC	2 cut(s)	698, 941
AfiI	CCNNNNNNNGG	2 cut(s)	630, 913
AflIII	ACRYGT	1 cut(s)	768
AgsI	TTSAA	5 cut(s)	72, 274, 541, 764, 1004
AhlI	ACTAGT	1 cut(s)	59
AjiI	CACGTC	1 cut(s)	769
AjnI	CCWGG	1 cut(s)	567
AloI	GAACNNNNNNTCC	2 cut(s)	605, 637
AluBI	AGCT	6 cut(s)	20, 365, 383, 437, 544, 835
AluI	AGCT	6 cut(s)	20, 365, 383, 437, 544, 835
Alw26I	GTCTC	1 cut(s)	920
AoxI	GGCC	3 cut(s)	106, 570, 646
ApeKI	GCWGC	4 cut(s)	11, 47, 164, 362
ApoI	RAATTY	2 cut(s)	335, 536
ArsI	GACNNNNNNTTYG	2 cut(s)	966, 998
Asp700I	GAANNNNTTC	1 cut(s)	21
AspA2I	CCTAGG	1 cut(s)	112
AspLEI	GCGC	1 cut(s)	748
AspS9I	GGNCC	3 cut(s)	601, 647, 667
AsuHPI	GGTGA	4 cut(s)	88, 367, 649, 727
AvaII	GGWCC	2 cut(s)	601, 667
AvrII	CCTAGG	1 cut(s)	112
BaeGI	GKGCMC	1 cut(s)	391
BanII	GRGCYC	1 cut(s)	592
BbsI	GAAGAC	1 cut(s)	771
BbvI	GCAGC	4 cut(s)	23, 34, 151, 374
BccI	CCATC	2 cut(s)	632, 668
BceAI	ACGGC	2 cut(s)	702, 812
BcgI	CGANNNNNNTGC	4 cut(s)	443, 453, 477, 487
BciT130I	CCWGG	1 cut(s)	569
BcoDI	GTCTC	1 cut(s)	920
BcuI	ACTAGT	1 cut(s)	59
BfaI	CTAG	4 cut(s)	60, 113, 593, 644
BfmI	CTRYAG	2 cut(s)	9, 720
BglI	GCCNNNNNGGC	1 cut(s)	114
BglII	AGATCT	1 cut(s)	724
BisI	GCNGC	5 cut(s)	12, 48, 109, 165, 363
BlnI	CCTAGG	1 cut(s)	112
BlsI	GCNGC	5 cut(s)	13, 49, 110, 166, 364
Bme1390I	CCNGG	1 cut(s)	569
Bme18I	GGWCC	2 cut(s)	601, 667
BmgBI	CACGTC	1 cut(s)	769
BmgT120I	GGNCC	3 cut(s)	601, 647, 667
BmiI	GGNNCC	3 cut(s)	6, 649, 669
BmrFI	CCNGG	1 cut(s)	569
BmsI	GCATC	6 cut(s)	34, 88, 187, 269, 473, 814
BoxI	GACNNNNGTC	1 cut(s)	83
BpiI	GAAGAC	1 cut(s)	771
BplI	GAGNNNNNCTC	2 cut(s)	349, 381
BpmI	CTGGAG	1 cut(s)	532
BpuEI	CTTGAG	1 cut(s)	713
BsaAI	YACGTR	1 cut(s)	790
BsaHI	GRCGYC	2 cut(s)	267, 476
BsaJI	CCNNGG	4 cut(s)	112, 490, 567, 604
BsaXI	ACNNNNNCTCC	2 cut(s)	542, 572
Bsc4I	CCNNNNNNNGG	2 cut(s)	630, 913
Bse1I	ACTGG	1 cut(s)	515
Bse3DI	GCAATG	1 cut(s)	603
BseBI	CCWGG	1 cut(s)	569
BseDI	CCNNGG	4 cut(s)	112, 490, 567, 604
BseGI	GGATG	5 cut(s)	103, 284, 422, 464, 773
BseLI	CCNNNNNNNGG	2 cut(s)	630, 913
BseMI	GCAATG	1 cut(s)	603
BseMII	CTCAG	1 cut(s)	681
BseNI	ACTGG	1 cut(s)	515
BseRI	GAGGAG	2 cut(s)	472, 932
BseSI	GKGCMC	1 cut(s)	391
BseX3I	CGGCCG	1 cut(s)	106
BseXI	GCAGC	4 cut(s)	23, 34, 151, 374
BsgI	GTGCAG	1 cut(s)	150
Bsh1236I	CGCG	2 cut(s)	746, 1011
Bsh1285I	CGRYCG	2 cut(s)	109, 457
BshFI	GGCC	3 cut(s)	108, 572, 648
BsiEI	CGRYCG	2 cut(s)	109, 457
BsiSI	CCGG	1 cut(s)	105
BslFI	GGGAC	2 cut(s)	464, 472
BslI	CCNNNNNNNGG	2 cut(s)	630, 913
BsmAI	GTCTC	1 cut(s)	920
BsmFI	GGGAC	2 cut(s)	464, 472
BsnI	GGCC	3 cut(s)	108, 572, 648
Bsp1286I	GDGCHC	2 cut(s)	391, 592
Bsp143I	GATC	2 cut(s)	652, 724
BspACI	CCGC	4 cut(s)	109, 133, 841, 1009
BspANI	GGCC	3 cut(s)	108, 572, 648
BspCNI	CTCAG	1 cut(s)	682
BspFNI	CGCG	2 cut(s)	746, 1011
BspLI	GGNNCC	3 cut(s)	6, 649, 669
BspMAI	CTGCAG	1 cut(s)	13
BsrDI	GCAATG	1 cut(s)	603
BsrI	ACTGG	1 cut(s)	515
BssECI	CCNNGG	4 cut(s)	112, 490, 567, 604
BssMI	GATC	2 cut(s)	652, 724
BssNI	GRCGYC	2 cut(s)	267, 476
BssT1I	CCWWGG	1 cut(s)	112
Bst2UI	CCWGG	1 cut(s)	569
Bst4CI	ACNGT	3 cut(s)	85, 458, 721
BstACI	GRCGYC	2 cut(s)	267, 476
BstAPI	GCANNNNNTGC	1 cut(s)	161
BstBAI	YACGTR	1 cut(s)	790
BstC8I	GCNNGC	4 cut(s)	552, 837, 841, 845
BstDEI	CTNAG	1 cut(s)	690
BstDSI	CCRYGG	1 cut(s)	490
BstEII	GGTNACC	1 cut(s)	76
BstF5I	GGATG	5 cut(s)	103, 284, 422, 464, 773
BstFNI	CGCG	2 cut(s)	746, 1011
BstHHI	GCGC	1 cut(s)	748
BstKTI	GATC	2 cut(s)	655, 727
BstMAI	GTCTC	1 cut(s)	920
BstMBI	GATC	2 cut(s)	652, 724
BstMCI	CGRYCG	2 cut(s)	109, 457
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 17, 114, 161
BstNI	CCWGG	1 cut(s)	569
BstNSI	RCATGY	1 cut(s)	847
BstPAI	GACNNNNGTC	1 cut(s)	83
BstPI	GGTNACC	1 cut(s)	76
BstSCI	CCNGG	1 cut(s)	567
BstSFI	CTRYAG	2 cut(s)	9, 720
BstSLI	GKGCMC	1 cut(s)	391
BstSNI	TACGTA	1 cut(s)	790
BstUI	CGCG	2 cut(s)	746, 1011
BstV1I	GCAGC	4 cut(s)	23, 34, 151, 374
BstV2I	GAAGAC	1 cut(s)	771
BstX2I	RGATCY	1 cut(s)	724
BstXI	CCANNNNNNTGG	1 cut(s)	568
BstYI	RGATCY	1 cut(s)	724
BstZI	CGGCCG	1 cut(s)	106
BsuRI	GGCC	3 cut(s)	108, 572, 648
BtgI	CCRYGG	1 cut(s)	490
BtgZI	GCGATG	1 cut(s)	531
BtrI	CACGTC	1 cut(s)	769
BtsCI	GGATG	5 cut(s)	103, 284, 422, 464, 773
BtsI	GCAGTG	2 cut(s)	502, 993
BtsIMutI	CAGTG	2 cut(s)	502, 993
Cac8I	GCNNGC	4 cut(s)	552, 837, 841, 845
CfoI	GCGC	1 cut(s)	748
Cfr13I	GGNCC	3 cut(s)	601, 647, 667
CseI	GACGC	5 cut(s)	79, 90, 275, 465, 752
Csp6I	GTAC	2 cut(s)	697, 940
CviAII	CATG	6 cut(s)	66, 94, 161, 616, 844, 979
CviQI	GTAC	2 cut(s)	697, 940
DdeI	CTNAG	1 cut(s)	690
DpnI	GATC	2 cut(s)	654, 726
DpnII	GATC	2 cut(s)	652, 724
EaeI	YGGCCR	2 cut(s)	106, 570
EagI	CGGCCG	1 cut(s)	106
EclXI	CGGCCG	1 cut(s)	106
Eco105I	TACGTA	1 cut(s)	790
Eco130I	CCWWGG	1 cut(s)	112
Eco24I	GRGCYC	1 cut(s)	592
Eco47I	GGWCC	2 cut(s)	601, 667
Eco52I	CGGCCG	1 cut(s)	106
Eco57I	CTGAAG	1 cut(s)	297
Eco91I	GGTNACC	1 cut(s)	76
EcoO109I	RGGNCCY	1 cut(s)	647
EcoO65I	GGTNACC	1 cut(s)	76
EcoRII	CCWGG	1 cut(s)	567
EcoT14I	CCWWGG	1 cut(s)	112
EcoT22I	ATGCAT	1 cut(s)	466
EcoT38I	GRGCYC	1 cut(s)	592
ErhI	CCWWGG	1 cut(s)	112
FaeI	CATG	6 cut(s)	69, 97, 164, 619, 847, 982
FaqI	GGGAC	2 cut(s)	464, 472
FatI	CATG	6 cut(s)	65, 93, 160, 615, 843, 978
FauI	CCCGC	1 cut(s)	848
FblI	GTMKAC	1 cut(s)	984
Fnu4HI	GCNGC	5 cut(s)	12, 48, 109, 165, 363
FokI	GGATG	5 cut(s)	110, 291, 409, 451, 760
FriOI	GRGCYC	1 cut(s)	592
Fsp4HI	GCNGC	5 cut(s)	12, 48, 109, 165, 363
FspBI	CTAG	4 cut(s)	60, 113, 593, 644
GlaI	GCGC	1 cut(s)	747
GluI	GCNGC	5 cut(s)	12, 48, 109, 165, 363
GsuI	CTGGAG	1 cut(s)	532
HaeIII	GGCC	3 cut(s)	108, 572, 648
HapII	CCGG	1 cut(s)	105
HgaI	GACGC	5 cut(s)	79, 90, 275, 465, 752
HhaI	GCGC	1 cut(s)	748
Hin1I	GRCGYC	2 cut(s)	267, 476
Hin1II	CATG	6 cut(s)	69, 97, 164, 619, 847, 982
Hin6I	GCGC	1 cut(s)	746
HinP1I	GCGC	1 cut(s)	746
HincII	GTYRAC	1 cut(s)	985
HindII	GTYRAC	1 cut(s)	985
HindIII	AAGCTT	1 cut(s)	381
HinfI	GANTC	1 cut(s)	804
HpaII	CCGG	1 cut(s)	105
HphI	GGTGA	4 cut(s)	88, 367, 649, 727
Hpy166II	GTNNAC	5 cut(s)	130, 265, 742, 940, 985
Hpy188I	TCNGA	3 cut(s)	238, 316, 678
Hpy8I	GTNNAC	5 cut(s)	130, 265, 742, 940, 985
Hpy99I	CGWCG	1 cut(s)	475
HpyAV	CCTTC	4 cut(s)	310, 321, 867, 1010
HpyCH4III	ACNGT	3 cut(s)	85, 458, 721
HpyCH4IV	ACGT	2 cut(s)	768, 789
HpyCH4V	TGCA	5 cut(s)	11, 142, 155, 167, 464
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 17, 114, 161
HpyF3I	CTNAG	1 cut(s)	690
HpySE526I	ACGT	2 cut(s)	768, 789
Hsp92I	GRCGYC	2 cut(s)	267, 476
Hsp92II	CATG	6 cut(s)	69, 97, 164, 619, 847, 982
HspAI	GCGC	1 cut(s)	746
Kzo9I	GATC	2 cut(s)	652, 724
LmnI	GCTCC	2 cut(s)	10, 513
LpnPI	CCDG	7 cut(s)	21, 118, 362, 496, 532, 554, 581
Lsp1109I	GCAGC	4 cut(s)	23, 34, 151, 374
LweI	GCATC	6 cut(s)	34, 88, 187, 269, 473, 814
MaeI	CTAG	4 cut(s)	60, 113, 593, 644
MaeII	ACGT	2 cut(s)	768, 789
MaeIII	GTNAC	5 cut(s)	76, 430, 497, 715, 956
MalI	GATC	2 cut(s)	654, 726
MboI	GATC	2 cut(s)	652, 724
MboII	GAAGA	5 cut(s)	223, 226, 776, 795, 961
MfeI	CAATTG	1 cut(s)	811
MflI	RGATCY	1 cut(s)	724
MhlI	GDGCHC	2 cut(s)	391, 592
MluCI	AATT	6 cut(s)	200, 218, 335, 523, 536, 811
MlyI	GAGTC	1 cut(s)	813
Mph1103I	ATGCAT	1 cut(s)	466
MroXI	GAANNNNTTC	1 cut(s)	21
MseI	TTAA	1 cut(s)	1015
MslI	CAYNNNNRTG	1 cut(s)	822
MspA1I	CMGCKG	1 cut(s)	437
MspI	CCGG	1 cut(s)	105
MspR9I	CCNGG	1 cut(s)	569
MunI	CAATTG	1 cut(s)	811
MvaI	CCWGG	1 cut(s)	569
MvnI	CGCG	2 cut(s)	746, 1011
MwoI	GCNNNNNNNGC	4 cut(s)	11, 17, 114, 161
NdeII	GATC	2 cut(s)	652, 724
NlaIII	CATG	6 cut(s)	69, 97, 164, 619, 847, 982
NlaIV	GGNNCC	3 cut(s)	6, 649, 669
NmeAIII	GCCGAG	1 cut(s)	598
NmuCI	GTSAC	3 cut(s)	76, 497, 715
NsiI	ATGCAT	1 cut(s)	466
NspI	RCATGY	1 cut(s)	847
PaeI	GCATGC	1 cut(s)	847
PcsI	WCGNNNNNNNCGW	2 cut(s)	786, 795
PdmI	GAANNNNTTC	1 cut(s)	21
PflFI	GACNNNGTC	1 cut(s)	803
PflMI	CCANNNNNTGG	1 cut(s)	913
PkrI	GCNGC	5 cut(s)	13, 49, 110, 166, 364
PleI	GAGTC	1 cut(s)	812
PpsI	GAGTC	1 cut(s)	812
Ppu21I	YACGTR	1 cut(s)	790
PshAI	GACNNNNGTC	1 cut(s)	83
Psp6I	CCWGG	1 cut(s)	567
PspEI	GGTNACC	1 cut(s)	76
PspGI	CCWGG	1 cut(s)	567
PspN4I	GGNNCC	3 cut(s)	6, 649, 669
PspPI	GGNCC	3 cut(s)	601, 647, 667
PstI	CTGCAG	1 cut(s)	13
PsuI	RGATCY	1 cut(s)	724
PsyI	GACNNNGTC	1 cut(s)	803
PvuII	CAGCTG	1 cut(s)	437
RsaI	GTAC	2 cut(s)	698, 941
RsaNI	GTAC	2 cut(s)	697, 940
RseI	CAYNNNNRTG	1 cut(s)	822
SalI	GTCGAC	1 cut(s)	983
SaqAI	TTAA	1 cut(s)	1015
SatI	GCNGC	5 cut(s)	12, 48, 109, 165, 363
Sau3AI	GATC	2 cut(s)	652, 724
Sau96I	GGNCC	3 cut(s)	601, 647, 667
SchI	GAGTC	1 cut(s)	813
ScrFI	CCNGG	1 cut(s)	569
SduI	GDGCHC	2 cut(s)	391, 592
SfaNI	GCATC	6 cut(s)	34, 88, 187, 269, 473, 814
SfcI	CTRYAG	2 cut(s)	9, 720
SinI	GGWCC	2 cut(s)	601, 667
SmiMI	CAYNNNNRTG	1 cut(s)	822
SmlI	CTYRAG	1 cut(s)	728
SmoI	CTYRAG	1 cut(s)	728
SnaBI	TACGTA	1 cut(s)	790
SpeI	ACTAGT	1 cut(s)	59
SphI	GCATGC	1 cut(s)	847
Sse9I	AATT	6 cut(s)	200, 218, 335, 523, 536, 811
SsiI	CCGC	4 cut(s)	109, 133, 841, 1009
SspMI	CTAG	4 cut(s)	60, 113, 593, 644
StyD4I	CCNGG	1 cut(s)	567
StyI	CCWWGG	1 cut(s)	112
TaaI	ACNGT	3 cut(s)	85, 458, 721
TaiI	ACGT	2 cut(s)	771, 792
TaqI	TCGA	3 cut(s)	303, 798, 984
TaqII	GACCGA	1 cut(s)	618
TasI	AATT	6 cut(s)	200, 218, 335, 523, 536, 811
TauI	GCSGC	1 cut(s)	111
Tru1I	TTAA	1 cut(s)	1015
Tru9I	TTAA	1 cut(s)	1015
TscAI	CASTG	2 cut(s)	502, 1000
TseFI	GTSAC	3 cut(s)	76, 497, 715
TseI	GCWGC	4 cut(s)	11, 47, 164, 362
Tsp45I	GTSAC	3 cut(s)	76, 497, 715
TspDTI	ATGAA	4 cut(s)	69, 348, 724, 858
TspGWI	ACGGA	4 cut(s)	221, 680, 745, 795
TspRI	CASTG	2 cut(s)	502, 1000
Tth111I	GACNNNGTC	1 cut(s)	803
Van91I	CCANNNNNTGG	1 cut(s)	913
VpaK11BI	GGWCC	2 cut(s)	601, 667
XapI	RAATTY	2 cut(s)	335, 536
XceI	RCATGY	1 cut(s)	847
XmaJI	CCTAGG	1 cut(s)	112
XmiI	GTMKAC	1 cut(s)	984
XmnI	GAANNNNTTC	1 cut(s)	21
XspI	CTAG	4 cut(s)	60, 113, 593, 644
Zsp2I	ATGCAT	1 cut(s)	466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.