Rh4AG129400

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001892
Physical Location & Seq
Forward (+)
28046287 .. 28048577
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG129400.1

Sequence Viewer

Length: 897 bp
ATGAAGTACGACAAACCCACAGTTTGTGTTCTTGATGCTTCAACCTACGTTGGCTTTTGGATTCTCAAAGGATTACTTGCTCGAGGCTTCAGAGTTCACGCAGCTACCCAGAAGAATGGGGAAACAGAGATTCAGAAGAGAATTAGGGAAATGGAGAAGGAAGAAGAGAGATTAGTAGTTTTCTCTGTTGATGTTTTGGATTATCATAGCATTGTTTTGGCTTTGAAAGGTTGCTCTGCTGTGTTCTGTTGTCTAGACAGTCCAGAAGCATACGATGAAAAAATGGTGAATTTGGAGGTGAGAGGAGCAATCAATGTTGTTGAAGCATGTGCTCAGACAGACAGCGTGGAGAAGATTTTGTTCTCTTCTTCATTGGCTGCAGCAATATGGAAGGAGGACATTTCTTCTGAGAAGGATGTGGATGAAAAGTCATGGAGTGATCCAGAATTCTGCAGGAAATTGAAGTTGTGGTATGCCTTAGCAAAGACACAATCGGAACAGGCTGCTTGGGCTCTATCAATGGACCGGACTCTTAAGATGGTCTCTATAAATTCTGGACTAGTCCTTGGGCCTAGTGTCACACAACAAAATCCGCGAACAACCATGTCATATCTTAACGGAGCTGCTCAAATGTATGAAAATGGTGTACTAGCGTTTGTCGACGTGGATTTTCTTGTAAATGTTCATATTCGAGCTCTAGAGGACCAGTCTGCTTGCGGGAGATATTTCTGCTTCAGTCACATGGTGAATACTGAGGACGAAGCTGTTAAGCTTGCACAGAGCTTAAGTCCTTTAATATCTTTACCACCAAGGTATCATTACGAAGGGAGTGAGGCACATATCGAGCGATTGAGGACTAAGAAGTTGAGCAAGCTGGTTGAGGGAGCTGCCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

298

Amino Acids

33.46

Weight (kDa)

5.51

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 125 6.3e-06 NmrA-like family
3Beta_HSD PF01073 11 - 230 5.9e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 13 - 234 4.8e-10 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 17 - 128 1.2e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 660
AccII CGCG 1 cut(s) 595
AciI CCGC 2 cut(s) 593, 717
AclWI GGATC 1 cut(s) 434
AcsI RAATTY 3 cut(s) 289, 446, 550
AcuI CTGAAG 2 cut(s) 73, 718
AdeI CACNNNGTG 1 cut(s) 745
AfaI GTAC 2 cut(s) 8, 648
AflII CTTAAG 2 cut(s) 533, 784
AgsI TTSAA 4 cut(s) 42, 226, 323, 463
AhlI ACTAGT 1 cut(s) 559
AjiI CACGTC 1 cut(s) 664
AluBI AGCT 8 cut(s) 104, 623, 695, 764, 772, 783, 874, 887
AluI AGCT 8 cut(s) 104, 623, 695, 764, 772, 783, 874, 887
Alw21I GWGCWC 2 cut(s) 334, 697
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 1 cut(s) 569
ApeKI GCWGC 6 cut(s) 101, 377, 380, 503, 623, 887
ApoI RAATTY 3 cut(s) 289, 446, 550
AspS9I GGNCC 3 cut(s) 523, 569, 703
AsuHPI GGTGA 3 cut(s) 298, 310, 757
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 2 cut(s) 523, 703
BaeI ACNNNNGTAYC 2 cut(s) 797, 830
BanII GRGCYC 2 cut(s) 514, 697
Bbv12I GWGCWC 2 cut(s) 334, 697
BbvI GCAGC 6 cut(s) 113, 364, 392, 490, 610, 874
BccI CCATC 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 547
BcuI ACTAGT 1 cut(s) 559
BfaI CTAG 5 cut(s) 254, 560, 573, 650, 698
BfmI CTRYAG 2 cut(s) 378, 451
BfrI CTTAAG 2 cut(s) 533, 784
BisI GCNGC 6 cut(s) 102, 378, 381, 504, 624, 888
BlsI GCNGC 6 cut(s) 103, 379, 382, 505, 625, 889
Bme18I GGWCC 2 cut(s) 523, 703
BmeT110I CYCGRG 1 cut(s) 81
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 3 cut(s) 523, 569, 703
BmsI GCATC 1 cut(s) 25
Bpu10I CCTNAGC 1 cut(s) 478
BsaI GGTCTC 1 cut(s) 547
BsaJI CCNNGG 2 cut(s) 565, 809
BsaWI WCCGGW 1 cut(s) 525
Bse1I ACTGG 1 cut(s) 706
BseDI CCNNGG 2 cut(s) 565, 809
BseGI GGATG 2 cut(s) 421, 427
BseMII CTCAG 3 cut(s) 347, 399, 744
BseNI ACTGG 1 cut(s) 706
BseRI GAGGAG 1 cut(s) 318
BseXI GCAGC 6 cut(s) 113, 364, 392, 490, 610, 874
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 1 cut(s) 571
BsiHKAI GWGCWC 2 cut(s) 334, 697
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 1 cut(s) 526
BsmAI GTCTC 1 cut(s) 547
BsnI GGCC 1 cut(s) 571
Bso31I GGTCTC 1 cut(s) 547
BsoBI CYCGRG 1 cut(s) 81
Bsp1286I GDGCHC 3 cut(s) 334, 514, 697
Bsp143I GATC 1 cut(s) 439
BspACI CCGC 2 cut(s) 593, 717
BspANI GGCC 1 cut(s) 571
BspCNI CTCAG 3 cut(s) 346, 400, 745
BspFNI CGCG 1 cut(s) 595
BspMAI CTGCAG 2 cut(s) 382, 455
BspPI GGATC 1 cut(s) 434
BspTI CTTAAG 2 cut(s) 533, 784
BspTNI GGTCTC 1 cut(s) 547
BsrI ACTGG 1 cut(s) 706
BssECI CCNNGG 2 cut(s) 565, 809
BssMI GATC 1 cut(s) 439
BssT1I CCWWGG 2 cut(s) 565, 809
Bst4CI ACNGT 2 cut(s) 22, 260
Bst6I CTCTTC 3 cut(s) 131, 159, 370
BstAFI CTTAAG 2 cut(s) 533, 784
BstC8I GCNNGC 3 cut(s) 715, 774, 872
BstDEI CTNAG 5 cut(s) 333, 408, 478, 753, 858
BstF5I GGATG 2 cut(s) 421, 427
BstFNI CGCG 1 cut(s) 595
BstKTI GATC 1 cut(s) 442
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 1 cut(s) 439
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNSI RCATGY 1 cut(s) 330
BstSFI CTRYAG 2 cut(s) 378, 451
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 6 cut(s) 113, 364, 392, 490, 610, 874
BstXI CCANNNNNNTGG 1 cut(s) 116
BsuRI GGCC 1 cut(s) 571
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 2 cut(s) 421, 427
Cac8I GCNNGC 3 cut(s) 715, 774, 872
Cfr13I GGNCC 3 cut(s) 523, 569, 703
Csp6I GTAC 2 cut(s) 7, 647
CviAII CATG 4 cut(s) 327, 432, 604, 742
CviQI GTAC 2 cut(s) 7, 647
DdeI CTNAG 5 cut(s) 333, 408, 478, 753, 858
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
DraIII CACNNNGTG 1 cut(s) 745
Eam1104I CTCTTC 3 cut(s) 131, 159, 370
EarI CTCTTC 3 cut(s) 131, 159, 370
Ecl136II GAGCTC 1 cut(s) 695
Eco130I CCWWGG 2 cut(s) 565, 809
Eco24I GRGCYC 2 cut(s) 514, 697
Eco31I GGTCTC 1 cut(s) 547
Eco47I GGWCC 2 cut(s) 523, 703
Eco53kI GAGCTC 1 cut(s) 695
Eco57I CTGAAG 2 cut(s) 73, 718
Eco88I CYCGRG 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 695
EcoRI GAATTC 1 cut(s) 446
EcoT14I CCWWGG 2 cut(s) 565, 809
EcoT38I GRGCYC 2 cut(s) 514, 697
ErhI CCWWGG 2 cut(s) 565, 809
FaeI CATG 4 cut(s) 330, 435, 607, 745
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FatI CATG 4 cut(s) 326, 431, 603, 741
FauI CCCGC 1 cut(s) 710
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 6 cut(s) 102, 378, 381, 504, 624, 888
FokI GGATG 2 cut(s) 428, 434
FriOI GRGCYC 2 cut(s) 514, 697
Fsp4HI GCNGC 6 cut(s) 102, 378, 381, 504, 624, 888
FspBI CTAG 5 cut(s) 254, 560, 573, 650, 698
GluI GCNGC 6 cut(s) 102, 378, 381, 504, 624, 888
HaeIII GGCC 1 cut(s) 571
HapII CCGG 1 cut(s) 526
Hin1II CATG 4 cut(s) 330, 435, 607, 745
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HindIII AAGCTT 1 cut(s) 770
HinfI GANTC 3 cut(s) 61, 130, 529
HpaII CCGG 1 cut(s) 526
HphI GGTGA 3 cut(s) 298, 310, 757
Hpy166II GTNNAC 3 cut(s) 97, 647, 661
Hpy188I TCNGA 5 cut(s) 92, 135, 336, 409, 496
Hpy188III TCNNGA 6 cut(s) 32, 254, 263, 443, 555, 698
Hpy8I GTNNAC 3 cut(s) 97, 647, 661
Hpy99I CGWCG 1 cut(s) 665
HpyAV CCTTC 4 cut(s) 151, 385, 406, 818
HpyCH4III ACNGT 2 cut(s) 22, 260
HpyCH4IV ACGT 2 cut(s) 48, 663
HpyCH4V TGCA 3 cut(s) 380, 453, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpyF3I CTNAG 5 cut(s) 333, 408, 478, 753, 858
HpySE526I ACGT 2 cut(s) 48, 663
Hsp92II CATG 4 cut(s) 330, 435, 607, 745
Kzo9I GATC 1 cut(s) 439
LmnI GCTCC 3 cut(s) 305, 620, 884
LpnPI CCDG 9 cut(s) 122, 276, 439, 456, 485, 539, 540, 719, 860
Lsp1109I GCAGC 6 cut(s) 113, 364, 392, 490, 610, 874
LweI GCATC 1 cut(s) 25
MaeI CTAG 5 cut(s) 254, 560, 573, 650, 698
MaeII ACGT 2 cut(s) 48, 663
MaeIII GTNAC 2 cut(s) 577, 737
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MboII GAAGA 8 cut(s) 124, 148, 173, 176, 357, 360, 364, 396
MhlI GDGCHC 3 cut(s) 334, 514, 697
MluCI AATT 5 cut(s) 141, 289, 446, 458, 550
MlyI GAGTC 1 cut(s) 523
MnlI CCTC 9 cut(s) 77, 289, 296, 388, 694, 748, 826, 846, 874
MseI TTAA 5 cut(s) 534, 615, 768, 785, 794
MspCI CTTAAG 2 cut(s) 533, 784
MspI CCGG 1 cut(s) 526
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 1 cut(s) 509
NdeII GATC 1 cut(s) 439
NlaIII CATG 4 cut(s) 330, 435, 607, 745
NmuCI GTSAC 2 cut(s) 577, 737
NspI RCATGY 1 cut(s) 330
PaeR7I CTCGAG 1 cut(s) 81
PfeI GAWTC 2 cut(s) 61, 130
PkrI GCNGC 6 cut(s) 103, 379, 382, 505, 625, 889
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
Psp124BI GAGCTC 1 cut(s) 697
PspPI GGNCC 3 cut(s) 523, 569, 703
PspXI VCTCGAGB 1 cut(s) 81
PstI CTGCAG 2 cut(s) 382, 455
RsaI GTAC 2 cut(s) 8, 648
RsaNI GTAC 2 cut(s) 7, 647
SacI GAGCTC 1 cut(s) 697
SalI GTCGAC 1 cut(s) 659
SaqAI TTAA 5 cut(s) 534, 615, 768, 785, 794
SatI GCNGC 6 cut(s) 102, 378, 381, 504, 624, 888
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 3 cut(s) 523, 569, 703
SchI GAGTC 1 cut(s) 523
SduI GDGCHC 3 cut(s) 334, 514, 697
SfaNI GCATC 1 cut(s) 25
SfcI CTRYAG 2 cut(s) 378, 451
Sfr274I CTCGAG 1 cut(s) 81
SinI GGWCC 2 cut(s) 523, 703
SlaI CTCGAG 1 cut(s) 81
SmlI CTYRAG 3 cut(s) 81, 533, 784
SmoI CTYRAG 3 cut(s) 81, 533, 784
SpeI ACTAGT 1 cut(s) 559
Sse9I AATT 5 cut(s) 141, 289, 446, 458, 550
SsiI CCGC 2 cut(s) 593, 717
SspMI CTAG 5 cut(s) 254, 560, 573, 650, 698
SstI GAGCTC 1 cut(s) 697
StyI CCWWGG 2 cut(s) 565, 809
TaaI ACNGT 2 cut(s) 22, 260
TaiI ACGT 2 cut(s) 51, 666
TaqI TCGA 4 cut(s) 82, 660, 691, 843
TasI AATT 5 cut(s) 141, 289, 446, 458, 550
TatI WGTACW 1 cut(s) 646
TfiI GAWTC 2 cut(s) 61, 130
Tru1I TTAA 5 cut(s) 534, 615, 768, 785, 794
Tru9I TTAA 5 cut(s) 534, 615, 768, 785, 794
TseFI GTSAC 2 cut(s) 577, 737
TseI GCWGC 6 cut(s) 101, 377, 380, 503, 623, 887
Tsp45I GTSAC 2 cut(s) 577, 737
TspDTI ATGAA 6 cut(s) 17, 291, 360, 438, 651, 674
TspGWI ACGGA 1 cut(s) 633
Vha464I CTTAAG 2 cut(s) 533, 784
VpaK11BI GGWCC 2 cut(s) 523, 703
XapI RAATTY 3 cut(s) 289, 446, 550
XbaI TCTAGA 2 cut(s) 253, 697
XceI RCATGY 1 cut(s) 330
XhoI CTCGAG 1 cut(s) 81
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 5 cut(s) 254, 560, 573, 650, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.