Rh7AG252500

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001892
Physical Location & Seq
Reverse (-)
25258645 .. 25261106
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG252500.1

Sequence Viewer

Length: 948 bp
ATGGCTCCCGCAGCTTCGAATCCTTTCGACGACCACCACGGCACCACGAGCTCGAACACAGTCTGCGTAATGGATGCGTCAGGCCACCTCGGCTCGACCCTCGTCCAGCGCCTGGTTCAGACGGGATACACAGTCCACGCCGCGCTTCAGAGCCATGGGGAATCCAAGTTGGGTTCGGTTTCTTGTGAGAATAACAAGAGGCTGAAGATTTTCCATTTAGACCCATTTGATTACCAGAGCATAGTTGATGCTTTGAAGGGCTGCTCTGGCTTGTTCTACACTTTTGAACCTCCACAAGATCAGCCCGACTATGATGAATATATGGCAGAAGTAGAGGTGAGAGCTGCACACAACGTTCTAGAAGCATGTGCCCGAACGGAAACCATAGACAAGGTTGTGTTCACATCATCAGTCACCGCCGTCGTTTGGAGCAGTAACAATAAATCGGTCGCTGCTACGCCGGAGTTGGACGAACGGCACTGGACTGACGTCAACTTCTGTCGCAAGTTCAAGCTATGGCATGCCCTATCAAAAACCCTAGCAGAGAAGACAGCGTGGGCGTTGGCAATGGATAGGAGCTTGAATATGGTGTCTATGAATGCAGGGTTGATGATAAATCCCGACTTGTCCATCAGCAACCCGTACTTGAAGGGAGCGGCGGAGATGTATGAAGACGGGCTGCTCGTGACCGTCGATATAAATTTCTTAGTGGATGCCCATATTTGCGTCTTCGAAGACATCACAGCCTACGGTCGATACGTATGTTTCAATAAAATCATCACTCAGTCGGGAGACGCTGTTGACCTTGCCCGGAAGTTGACCCCTTCTGCCCCGGCCTCGTTGCCCCAAAGCAAGGACCAGGATATGAGAATCATCCAAGAGAGAATAAGCAACAAGAAGTTGAATAAGTTGATGGTGGGTTTCAAGAGTAAATCACAAATGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

315

Amino Acids

34.88

Weight (kDa)

5.8

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 18 - 141 1.8e-07 NmrA-like family
3Beta_HSD PF01073 23 - 183 6.6e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 24 - 200 1.1e-07 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 26 - 141 1e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 41
AccB7I CCANNNNNTGG 1 cut(s) 112
AccBSI CCGCTC 1 cut(s) 656
AccII CGCG 1 cut(s) 143
AciI CCGC 5 cut(s) 9, 141, 417, 656, 659
AclI AACGTT 1 cut(s) 354
AcsI RAATTY 1 cut(s) 700
AcuI CTGAAG 2 cut(s) 131, 224
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 3 cut(s) 112, 426, 853
AgsI TTSAA 8 cut(s) 256, 287, 511, 583, 649, 769, 904, 925
AjnI CCWGG 2 cut(s) 111, 858
AleI CACNNNNGTG 1 cut(s) 940
AluBI AGCT 5 cut(s) 14, 51, 344, 514, 579
AluI AGCT 5 cut(s) 14, 51, 344, 514, 579
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 1 cut(s) 786
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 2 cut(s) 82, 834
ApeKI GCWGC 5 cut(s) 11, 261, 344, 452, 679
ApoI RAATTY 1 cut(s) 700
Asp700I GAANNNNTTC 2 cut(s) 23, 209
AspLEI GCGC 2 cut(s) 111, 145
AspS9I GGNCC 1 cut(s) 856
AsuC2I CCSGG 2 cut(s) 811, 833
AsuHPI GGTGA 2 cut(s) 349, 406
AsuII TTCGAA 2 cut(s) 17, 732
AvaII GGWCC 1 cut(s) 856
BaeGI GKGCMC 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 748, 781
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 53
BauI CACGAG 2 cut(s) 46, 683
BbsI GAAGAC 4 cut(s) 554, 678, 721, 741
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 5 cut(s) 23, 248, 331, 439, 666
BccI CCATC 2 cut(s) 638, 907
BceAI ACGGC 3 cut(s) 55, 404, 491
BciT130I CCWGG 2 cut(s) 113, 860
BciVI GTATCC 1 cut(s) 119
BcnI CCSGG 2 cut(s) 811, 833
BcoDI GTCTC 1 cut(s) 786
BfaI CTAG 2 cut(s) 359, 539
BfoI RGCGCY 1 cut(s) 112
BfuI GTATCC 1 cut(s) 119
BglI GCCNNNNNGGC 1 cut(s) 90
BisI GCNGC 7 cut(s) 12, 141, 262, 345, 453, 657, 680
BlsI GCNGC 7 cut(s) 13, 142, 263, 346, 454, 658, 681
Bme1390I CCNGG 4 cut(s) 113, 811, 833, 860
Bme18I GGWCC 1 cut(s) 856
BmgT120I GGNCC 1 cut(s) 856
BmiI GGNNCC 2 cut(s) 6, 43
BmrFI CCNGG 4 cut(s) 113, 811, 833, 860
BmsI GCATC 3 cut(s) 64, 238, 703
BoxI GACNNNNGTC 2 cut(s) 101, 488
BpiI GAAGAC 4 cut(s) 554, 678, 721, 741
Bpu14I TTCGAA 2 cut(s) 17, 732
BpuMI CCSGG 2 cut(s) 811, 833
BsaAI YACGTR 1 cut(s) 760
BsaHI GRCGYC 1 cut(s) 489
BsaJI CCNNGG 4 cut(s) 37, 88, 154, 831
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 426, 853
Bse1I ACTGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 573
BseBI CCWGG 2 cut(s) 113, 860
BseDI CCNNGG 4 cut(s) 37, 88, 154, 831
BseGI GGATG 3 cut(s) 79, 718, 873
BseLI CCNNNNNNNGG 3 cut(s) 112, 426, 853
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 1 cut(s) 797
BseNI ACTGG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 5 cut(s) 23, 248, 331, 439, 666
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 2 cut(s) 450, 754
BshFI GGCC 2 cut(s) 84, 836
BshNI GGYRCC 1 cut(s) 41
BsiEI CGRYCG 2 cut(s) 450, 754
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 3 cut(s) 461, 811, 833
BslI CCNNNNNNNGG 3 cut(s) 112, 426, 853
BsmAI GTCTC 1 cut(s) 786
BsmBI CGTCTC 1 cut(s) 786
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 2 cut(s) 84, 836
Bsp119I TTCGAA 2 cut(s) 17, 732
Bsp1286I GDGCHC 2 cut(s) 53, 373
Bsp143I GATC 1 cut(s) 298
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 5 cut(s) 9, 141, 417, 656, 659
BspANI GGCC 2 cut(s) 84, 836
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 2 cut(s) 6, 43
BspT104I TTCGAA 2 cut(s) 17, 732
BspT107I GGYRCC 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 656
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 4 cut(s) 37, 88, 154, 831
BssMI GATC 1 cut(s) 298
BssNI GRCGYC 1 cut(s) 489
BssSI CACGAG 2 cut(s) 46, 683
BssT1I CCWWGG 1 cut(s) 154
Bst2BI CACGAG 2 cut(s) 46, 683
Bst2UI CCWGG 2 cut(s) 113, 860
Bst4CI ACNGT 4 cut(s) 61, 133, 691, 752
BstACI GRCGYC 1 cut(s) 489
BstBAI YACGTR 1 cut(s) 760
BstBI TTCGAA 2 cut(s) 17, 732
BstC8I GCNNGC 1 cut(s) 522
BstDEI CTNAG 2 cut(s) 706, 783
BstDSI CCRYGG 2 cut(s) 37, 154
BstF5I GGATG 3 cut(s) 79, 718, 873
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 2 cut(s) 111, 145
BstKTI GATC 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 786
BstMBI GATC 1 cut(s) 298
BstMCI CGRYCG 2 cut(s) 450, 754
BstMWI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
BstNI CCWGG 2 cut(s) 113, 860
BstNSI RCATGY 2 cut(s) 369, 524
BstPAI GACNNNNGTC 2 cut(s) 101, 488
BstSCI CCNGG 4 cut(s) 111, 809, 831, 858
BstSLI GKGCMC 1 cut(s) 373
BstSNI TACGTA 1 cut(s) 760
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 5 cut(s) 23, 248, 331, 439, 666
BstV2I GAAGAC 4 cut(s) 554, 678, 721, 741
BsuI GTATCC 1 cut(s) 119
BsuRI GGCC 2 cut(s) 84, 836
BtgI CCRYGG 2 cut(s) 37, 154
BtsCI GGATG 3 cut(s) 79, 718, 873
BtsIMutI CAGTG 1 cut(s) 478
Cac8I GCNNGC 1 cut(s) 522
CaiI CAGNNNCTG 1 cut(s) 112
CfoI GCGC 2 cut(s) 111, 145
Cfr13I GGNCC 1 cut(s) 856
CseI GACGC 3 cut(s) 66, 715, 803
Csp6I GTAC 1 cut(s) 643
CviAII CATG 3 cut(s) 155, 366, 521
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 2 cut(s) 706, 783
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
EciI GGCGGA 1 cut(s) 674
Ecl136II GAGCTC 1 cut(s) 51
Eco105I TACGTA 1 cut(s) 760
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 856
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 131, 224
EcoICRI GAGCTC 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 111, 858
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 786
FaeI CATG 3 cut(s) 158, 369, 524
FatI CATG 3 cut(s) 154, 365, 520
FauI CCCGC 1 cut(s) 16
Fnu4HI GCNGC 7 cut(s) 12, 141, 262, 345, 453, 657, 680
FokI GGATG 3 cut(s) 86, 725, 860
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 7 cut(s) 12, 141, 262, 345, 453, 657, 680
FspBI CTAG 2 cut(s) 359, 539
GlaI GCGC 2 cut(s) 110, 144
GluI GCNGC 7 cut(s) 12, 141, 262, 345, 453, 657, 680
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 2 cut(s) 84, 836
HapII CCGG 3 cut(s) 461, 811, 833
HgaI GACGC 3 cut(s) 66, 715, 803
HhaI GCGC 2 cut(s) 111, 145
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 3 cut(s) 158, 369, 524
Hin6I GCGC 2 cut(s) 109, 143
HinP1I GCGC 2 cut(s) 109, 143
HincII GTYRAC 3 cut(s) 493, 802, 819
HindII GTYRAC 3 cut(s) 493, 802, 819
HinfI GANTC 3 cut(s) 19, 161, 870
HpaII CCGG 3 cut(s) 461, 811, 833
HphI GGTGA 2 cut(s) 349, 406
Hpy166II GTNNAC 5 cut(s) 136, 402, 493, 802, 819
Hpy188I TCNGA 2 cut(s) 120, 150
Hpy188III TCNNGA 5 cut(s) 359, 620, 685, 789, 925
Hpy8I GTNNAC 5 cut(s) 136, 402, 493, 802, 819
Hpy99I CGWCG 3 cut(s) 32, 425, 695
HpyAV CCTTC 3 cut(s) 250, 643, 834
HpyCH4III ACNGT 4 cut(s) 61, 133, 691, 752
HpyCH4IV ACGT 3 cut(s) 354, 489, 759
HpyCH4V TGCA 2 cut(s) 347, 602
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
HpyF3I CTNAG 2 cut(s) 706, 783
HpySE526I ACGT 3 cut(s) 354, 489, 759
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 3 cut(s) 158, 369, 524
HspAI GCGC 2 cut(s) 109, 143
Kzo9I GATC 1 cut(s) 298
LmnI GCTCC 4 cut(s) 10, 429, 576, 653
Lsp1109I GCAGC 5 cut(s) 23, 248, 331, 439, 666
LweI GCATC 3 cut(s) 64, 238, 703
MaeI CTAG 2 cut(s) 359, 539
MaeII ACGT 3 cut(s) 354, 489, 759
MaeIII GTNAC 3 cut(s) 412, 434, 685
MalI GATC 1 cut(s) 300
MbiI CCGCTC 1 cut(s) 656
MboI GATC 1 cut(s) 298
MboII GAAGA 5 cut(s) 217, 559, 683, 721, 746
MhlI GDGCHC 2 cut(s) 53, 373
MluCI AATT 1 cut(s) 700
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 6 cut(s) 98, 110, 192, 300, 328, 847
MroXI GAANNNNTTC 2 cut(s) 23, 209
MslI CAYNNNNRTG 1 cut(s) 940
MspI CCGG 3 cut(s) 461, 811, 833
MspR9I CCNGG 4 cut(s) 113, 811, 833, 860
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 2 cut(s) 113, 860
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
NciI CCSGG 2 cut(s) 811, 833
NcoI CCATGG 1 cut(s) 154
NdeII GATC 1 cut(s) 298
NlaIII CATG 3 cut(s) 158, 369, 524
NlaIV GGNNCC 2 cut(s) 6, 43
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 412, 685
NspI RCATGY 2 cut(s) 369, 524
NspV TTCGAA 2 cut(s) 17, 732
OliI CACNNNNGTG 1 cut(s) 940
PaeI GCATGC 1 cut(s) 524
PcsI WCGNNNNNNNCGW 3 cut(s) 681, 690, 756
PctI GAATGC 1 cut(s) 604
PdmI GAANNNNTTC 2 cut(s) 23, 209
PfeI GAWTC 3 cut(s) 19, 161, 870
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 7 cut(s) 13, 142, 263, 346, 454, 658, 681
Ppu21I YACGTR 1 cut(s) 760
PshAI GACNNNNGTC 2 cut(s) 101, 488
Psp124BI GAGCTC 1 cut(s) 53
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 111, 858
PspGI CCWGG 2 cut(s) 111, 858
PspN4I GGNNCC 2 cut(s) 6, 43
PspPI GGNCC 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 112
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
RseI CAYNNNNRTG 1 cut(s) 940
SacI GAGCTC 1 cut(s) 53
SatI GCNGC 7 cut(s) 12, 141, 262, 345, 453, 657, 680
Sau3AI GATC 1 cut(s) 298
Sau96I GGNCC 1 cut(s) 856
ScrFI CCNGG 4 cut(s) 113, 811, 833, 860
SduI GDGCHC 2 cut(s) 53, 373
SfaNI GCATC 3 cut(s) 64, 238, 703
SfuI TTCGAA 2 cut(s) 17, 732
SinI GGWCC 1 cut(s) 856
SmiMI CAYNNNNRTG 1 cut(s) 940
SnaBI TACGTA 1 cut(s) 760
SphI GCATGC 1 cut(s) 524
Sse9I AATT 1 cut(s) 700
SsiI CCGC 5 cut(s) 9, 141, 417, 656, 659
SspMI CTAG 2 cut(s) 359, 539
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 4 cut(s) 111, 809, 831, 858
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 4 cut(s) 61, 133, 691, 752
TaiI ACGT 3 cut(s) 357, 492, 762
TaqI TCGA 7 cut(s) 17, 27, 53, 95, 693, 732, 754
TaqII GACCGA 1 cut(s) 436
TasI AATT 1 cut(s) 700
TauI GCSGC 2 cut(s) 143, 659
TfiI GAWTC 3 cut(s) 19, 161, 870
TscAI CASTG 1 cut(s) 485
TseFI GTSAC 2 cut(s) 412, 685
TseI GCWGC 5 cut(s) 11, 261, 344, 452, 679
Tsp45I GTSAC 2 cut(s) 412, 685
TspDTI ATGAA 3 cut(s) 330, 611, 684
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 485
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 856
XapI RAATTY 1 cut(s) 700
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 2 cut(s) 369, 524
XmnI GAANNNNTTC 2 cut(s) 23, 209
XspI CTAG 2 cut(s) 359, 539
ZraI GACGTC 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.