FvH4_5g22420

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001892
Physical Location & Seq
Reverse (-)
13784416 .. 13788442
4027 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22420.t1

Sequence Viewer

Length: 948 bp
ATGGCTCCCGCAGCTTCGGATCCTTTCGACGACCTCCACCACACCATGAGCTCCACCACAGTTTGCGTAATGGATGCGTCGGGCCACCTCGGCTCGACCCTCGTCCGGCGCCTTGTTCAGAGGGGCTACACAGTCCACGCTGCGCTCCAGAGCCATGGGGAATCGAAGTTGGGTTCGGTTTCGTTTGAGAATAACAAGAGGCTGAAGATTTTCCATTTAGACCCGTTTGATTACCAGAGCATAGTCGATGCTTTGAAGGGCTGTTCTGGCTTGTTCTACACCTTTGAGCCTCCACAAGACCAGCCCGACTATGATGAATATATGGCGGATGTAGAGGTAAGAGCGGCACACAACGTTCTAGAAGCATGTGCCCGAACCGAAACCATAGACAAGGTAGTGTTCACGTCCTCTGTAACCGCCGTCGTTTGGAGTAACAACAGATCGACGGCCGCTGCTACGCCGGAGTTGGACGAACGGCACTGGACTGACGTCAACTTCTGCCGCAAGGTCAAGCTATGGCATGCCCTATCAAAAACCCTAGCAGAGAAGACAGCGTGGGCAATGGCAATGGACAGGGGCTTGAATATGGTGTCCATGAATGTAGGGTTGATGTTGAACGCCGACTTGTCCATCAGCAACCCGTACTTGAAGGGAGCGGCCGAGATGTATGAAGACGGCGTGCTTGTCACCGTCGATGTTAATTTTGTGGTGGATGCTCATATTTGCGTTTTTGAAGACGTCACATCCTATGGTCGGTACTTATGTTTCAACAACATCATCACTCAATCGGAGGACGCCGTTAACCTTGCCCGGAAGTTGACGCCTTCCGCCCCGGCCGCGTTGCCCCAAGGCAAAGACCAGGATGTGAGAATCATACAAGAGAGAATCAGCAACAAGAAGTTGAATAAGTTGATGGTGGGTTACAAGAGTAAATCTCAAGTGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

316

Amino Acids

34.96

Weight (kDa)

5.8

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 19 - 146 7.5e-08 NmrA-like family
3Beta_HSD PF01073 23 - 151 2.5e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 24 - 201 4.7e-09 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 26 - 141 1.3e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 492, 741
AccB1I GGYRCC 1 cut(s) 108
AccBSI CCGCTC 2 cut(s) 344, 656
AccII CGCG 1 cut(s) 839
AciI CCGC 9 cut(s) 9, 326, 344, 417, 450, 502, 656, 828, 837
AclI AACGTT 1 cut(s) 354
AclWI GGATC 2 cut(s) 14, 27
AcoI YGGCCR 3 cut(s) 447, 657, 834
AcuI CTGAAG 1 cut(s) 224
AcyI GRCGYC 5 cut(s) 109, 489, 738, 795, 821
AfaI GTAC 2 cut(s) 644, 758
AfiI CCNNNNNNNGG 3 cut(s) 105, 426, 753
AgsI TTSAA 7 cut(s) 256, 583, 616, 649, 734, 769, 904
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 1 cut(s) 858
AluBI AGCT 3 cut(s) 14, 51, 514
AluI AGCT 3 cut(s) 14, 51, 514
Alw21I GWGCWC 1 cut(s) 53
AlwI GGATC 2 cut(s) 14, 27
AoxI GGCC 4 cut(s) 82, 447, 657, 834
ApeKI GCWGC 3 cut(s) 11, 140, 452
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 2 cut(s) 111, 145
AspS9I GGNCC 1 cut(s) 82
AsuC2I CCSGG 2 cut(s) 811, 833
AsuHPI GGTGA 1 cut(s) 679
BaeGI GKGCMC 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 748, 781
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 3 cut(s) 554, 678, 741
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 23, 127, 439
BccI CCATC 2 cut(s) 638, 907
BceAI ACGGC 5 cut(s) 404, 462, 491, 691, 782
BciT130I CCWGG 1 cut(s) 860
BcnI CCSGG 2 cut(s) 811, 833
BfaI CTAG 2 cut(s) 359, 539
BfoI RGCGCY 1 cut(s) 112
BglI GCCNNNNNGGC 1 cut(s) 90
BisI GCNGC 8 cut(s) 12, 141, 345, 450, 453, 502, 657, 837
BlsI GCNGC 8 cut(s) 13, 142, 346, 451, 454, 503, 658, 838
Bme1390I CCNGG 3 cut(s) 811, 833, 860
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 3 cut(s) 6, 21, 110
BmrFI CCNGG 3 cut(s) 811, 833, 860
BmsI GCATC 3 cut(s) 64, 238, 703
BoxI GACNNNNGTC 2 cut(s) 101, 488
BpiI GAAGAC 3 cut(s) 554, 678, 741
BplI GAGNNNNNCTC 1 cut(s) 919
BpmI CTGGAG 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 921
BpuMI CCSGG 2 cut(s) 811, 833
BsaHI GRCGYC 5 cut(s) 109, 489, 738, 795, 821
BsaJI CCNNGG 4 cut(s) 88, 154, 831, 847
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 426, 753
Bse1I ACTGG 1 cut(s) 485
Bse3DI GCAATG 2 cut(s) 567, 573
BseBI CCWGG 1 cut(s) 860
BseDI CCNNGG 4 cut(s) 88, 154, 831, 847
BseGI GGATG 5 cut(s) 79, 334, 718, 743, 868
BseLI CCNNNNNNNGG 3 cut(s) 105, 426, 753
BseMI GCAATG 2 cut(s) 567, 573
BseNI ACTGG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 373
BseX3I CGGCCG 3 cut(s) 447, 657, 834
BseXI GCAGC 3 cut(s) 23, 127, 439
Bsh1236I CGCG 1 cut(s) 839
Bsh1285I CGRYCG 3 cut(s) 450, 660, 837
BshFI GGCC 4 cut(s) 84, 449, 659, 836
BshNI GGYRCC 1 cut(s) 108
BsiEI CGRYCG 3 cut(s) 450, 660, 837
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 4 cut(s) 106, 461, 811, 833
BslI CCNNNNNNNGG 3 cut(s) 105, 426, 753
BsnI GGCC 4 cut(s) 84, 449, 659, 836
Bsp1286I GDGCHC 2 cut(s) 53, 373
Bsp143I GATC 2 cut(s) 19, 440
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 9 cut(s) 9, 326, 344, 417, 450, 502, 656, 828, 837
BspANI GGCC 4 cut(s) 84, 449, 659, 836
BspFNI CGCG 1 cut(s) 839
BspLI GGNNCC 3 cut(s) 6, 21, 110
BspPI GGATC 2 cut(s) 14, 27
BspT107I GGYRCC 1 cut(s) 108
BsrBI CCGCTC 2 cut(s) 344, 656
BsrDI GCAATG 2 cut(s) 567, 573
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 4 cut(s) 88, 154, 831, 847
BssMI GATC 2 cut(s) 19, 440
BssNI GRCGYC 5 cut(s) 109, 489, 738, 795, 821
BssT1I CCWWGG 2 cut(s) 154, 847
Bst2UI CCWGG 1 cut(s) 860
Bst4CI ACNGT 3 cut(s) 61, 133, 691
BstACI GRCGYC 5 cut(s) 109, 489, 738, 795, 821
BstC8I GCNNGC 2 cut(s) 522, 680
BstDSI CCRYGG 1 cut(s) 154
BstF5I GGATG 5 cut(s) 79, 334, 718, 743, 868
BstFNI CGCG 1 cut(s) 839
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 2 cut(s) 111, 145
BstKTI GATC 2 cut(s) 22, 443
BstMBI GATC 2 cut(s) 19, 440
BstMCI CGRYCG 3 cut(s) 450, 660, 837
BstMWI GCNNNNNNNGC 4 cut(s) 11, 90, 267, 836
BstNI CCWGG 1 cut(s) 860
BstNSI RCATGY 2 cut(s) 369, 524
BstPAI GACNNNNGTC 2 cut(s) 101, 488
BstSCI CCNGG 3 cut(s) 809, 831, 858
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 839
BstV1I GCAGC 3 cut(s) 23, 127, 439
BstV2I GAAGAC 3 cut(s) 554, 678, 741
BstX2I RGATCY 1 cut(s) 19
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 1 cut(s) 19
BstZI CGGCCG 3 cut(s) 447, 657, 834
BsuRI GGCC 4 cut(s) 84, 449, 659, 836
BtgI CCRYGG 1 cut(s) 154
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 5 cut(s) 79, 334, 718, 743, 868
BtsIMutI CAGTG 1 cut(s) 478
Cac8I GCNNGC 2 cut(s) 522, 680
CfoI GCGC 2 cut(s) 111, 145
Cfr13I GGNCC 1 cut(s) 82
CseI GACGC 3 cut(s) 66, 803, 829
Csp6I GTAC 2 cut(s) 643, 757
CviAII CATG 5 cut(s) 46, 155, 366, 521, 595
CviQI GTAC 2 cut(s) 643, 757
DinI GGCGCC 1 cut(s) 110
DpnI GATC 2 cut(s) 21, 442
DpnII GATC 2 cut(s) 19, 440
EaeI YGGCCR 3 cut(s) 447, 657, 834
EagI CGGCCG 3 cut(s) 447, 657, 834
EciI GGCGGA 2 cut(s) 341, 817
Ecl136II GAGCTC 1 cut(s) 51
EclXI CGGCCG 3 cut(s) 447, 657, 834
Eco130I CCWWGG 2 cut(s) 154, 847
Eco24I GRGCYC 1 cut(s) 53
Eco52I CGGCCG 3 cut(s) 447, 657, 834
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 858
EcoT14I CCWWGG 2 cut(s) 154, 847
EcoT38I GRGCYC 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 110
EheI GGCGCC 1 cut(s) 110
ErhI CCWWGG 2 cut(s) 154, 847
FaeI CATG 5 cut(s) 49, 158, 369, 524, 598
FatI CATG 5 cut(s) 45, 154, 365, 520, 594
FauI CCCGC 1 cut(s) 16
Fnu4HI GCNGC 8 cut(s) 12, 141, 345, 450, 453, 502, 657, 837
FokI GGATG 5 cut(s) 86, 341, 725, 730, 875
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 8 cut(s) 12, 141, 345, 450, 453, 502, 657, 837
FspBI CTAG 2 cut(s) 359, 539
GlaI GCGC 2 cut(s) 110, 144
GluI GCNGC 8 cut(s) 12, 141, 345, 450, 453, 502, 657, 837
GsuI CTGGAG 1 cut(s) 131
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 4 cut(s) 84, 449, 659, 836
HapII CCGG 4 cut(s) 106, 461, 811, 833
HgaI GACGC 3 cut(s) 66, 803, 829
HhaI GCGC 2 cut(s) 111, 145
Hin1I GRCGYC 5 cut(s) 109, 489, 738, 795, 821
Hin1II CATG 5 cut(s) 49, 158, 369, 524, 598
Hin6I GCGC 2 cut(s) 109, 143
HinP1I GCGC 2 cut(s) 109, 143
HincII GTYRAC 3 cut(s) 493, 802, 819
HindII GTYRAC 3 cut(s) 493, 802, 819
HinfI GANTC 3 cut(s) 161, 870, 885
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 4 cut(s) 106, 461, 811, 833
HphI GGTGA 1 cut(s) 679
Hpy166II GTNNAC 5 cut(s) 136, 402, 493, 802, 819
Hpy188I TCNGA 3 cut(s) 19, 120, 790
Hpy188III TCNNGA 2 cut(s) 148, 359
Hpy8I GTNNAC 5 cut(s) 136, 402, 493, 802, 819
Hpy99I CGWCG 5 cut(s) 32, 82, 425, 448, 695
HpyAV CCTTC 3 cut(s) 250, 643, 834
HpyCH4III ACNGT 3 cut(s) 61, 133, 691
HpyCH4IV ACGT 4 cut(s) 354, 404, 489, 738
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 90, 267, 836
HpySE526I ACGT 4 cut(s) 354, 404, 489, 738
Hsp92I GRCGYC 5 cut(s) 109, 489, 738, 795, 821
Hsp92II CATG 5 cut(s) 49, 158, 369, 524, 598
HspAI GCGC 2 cut(s) 109, 143
KasI GGCGCC 1 cut(s) 108
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 2 cut(s) 19, 440
LmnI GCTCC 4 cut(s) 10, 56, 150, 653
Lsp1109I GCAGC 3 cut(s) 23, 127, 439
LweI GCATC 3 cut(s) 64, 238, 703
MaeI CTAG 2 cut(s) 359, 539
MaeII ACGT 4 cut(s) 354, 404, 489, 738
MaeIII GTNAC 5 cut(s) 412, 431, 685, 739, 920
MalI GATC 2 cut(s) 21, 442
MbiI CCGCTC 2 cut(s) 344, 656
MboI GATC 2 cut(s) 19, 440
MboII GAAGA 4 cut(s) 217, 559, 683, 746
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 2 cut(s) 53, 373
MluCI AATT 1 cut(s) 700
Mly113I GGCGCC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 9 cut(s) 44, 98, 110, 114, 192, 300, 328, 418, 784
MroXI GAANNNNTTC 1 cut(s) 209
MseI TTAA 2 cut(s) 699, 801
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 4 cut(s) 106, 461, 811, 833
MspR9I CCNGG 3 cut(s) 811, 833, 860
MvaI CCWGG 1 cut(s) 860
MvnI CGCG 1 cut(s) 839
MwoI GCNNNNNNNGC 4 cut(s) 11, 90, 267, 836
NarI GGCGCC 1 cut(s) 109
NciI CCSGG 2 cut(s) 811, 833
NcoI CCATGG 1 cut(s) 154
NdeII GATC 2 cut(s) 19, 440
NlaIII CATG 5 cut(s) 49, 158, 369, 524, 598
NlaIV GGNNCC 3 cut(s) 6, 21, 110
NmeAIII GCCGAG 2 cut(s) 69, 685
NmuCI GTSAC 2 cut(s) 685, 739
NspI RCATGY 2 cut(s) 369, 524
PaeI GCATGC 1 cut(s) 524
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 3 cut(s) 161, 870, 885
PkrI GCNGC 8 cut(s) 13, 142, 346, 451, 454, 503, 658, 838
PluTI GGCGCC 1 cut(s) 112
PshAI GACNNNNGTC 2 cut(s) 101, 488
Psp124BI GAGCTC 1 cut(s) 53
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 858
PspGI CCWGG 1 cut(s) 858
PspN4I GGNNCC 3 cut(s) 6, 21, 110
PspPI GGNCC 1 cut(s) 82
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 2 cut(s) 644, 758
RsaNI GTAC 2 cut(s) 643, 757
SacI GAGCTC 1 cut(s) 53
SaqAI TTAA 2 cut(s) 699, 801
SatI GCNGC 8 cut(s) 12, 141, 345, 450, 453, 502, 657, 837
Sau3AI GATC 2 cut(s) 19, 440
Sau96I GGNCC 1 cut(s) 82
ScrFI CCNGG 3 cut(s) 811, 833, 860
SduI GDGCHC 2 cut(s) 53, 373
SfaNI GCATC 3 cut(s) 64, 238, 703
SfoI GGCGCC 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 936
SmoI CTYRAG 1 cut(s) 936
SphI GCATGC 1 cut(s) 524
Sse9I AATT 1 cut(s) 700
SsiI CCGC 9 cut(s) 9, 326, 344, 417, 450, 502, 656, 828, 837
SspDI GGCGCC 1 cut(s) 108
SspMI CTAG 2 cut(s) 359, 539
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 3 cut(s) 809, 831, 858
StyI CCWWGG 2 cut(s) 154, 847
TaaI ACNGT 3 cut(s) 61, 133, 691
TaiI ACGT 4 cut(s) 357, 407, 492, 741
TaqI TCGA 6 cut(s) 27, 95, 164, 246, 443, 693
TasI AATT 1 cut(s) 700
TauI GCSGC 5 cut(s) 347, 452, 504, 659, 839
TfiI GAWTC 3 cut(s) 161, 870, 885
Tru1I TTAA 2 cut(s) 699, 801
Tru9I TTAA 2 cut(s) 699, 801
TscAI CASTG 1 cut(s) 485
TseFI GTSAC 2 cut(s) 685, 739
TseI GCWGC 3 cut(s) 11, 140, 452
Tsp45I GTSAC 2 cut(s) 685, 739
TspDTI ATGAA 3 cut(s) 330, 611, 684
TspRI CASTG 1 cut(s) 485
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 2 cut(s) 369, 524
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 2 cut(s) 359, 539
ZraI GACGTC 2 cut(s) 490, 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.