Rw0G002460

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00073
OG0001892
Physical Location & Seq
Reverse (-)
68716 .. 70870
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002460.1

Sequence Viewer

Length: 951 bp
ATGGCTCCCGCAGCTTCGAATCCTTTCGACGACCACCACGGCACCACGAGCTCGAACACAGTCTGCGTAATGGATGCGTCAGGCCACCTCGGCTCGACCCTCGTCCAGCGCCTGGTTCAGACGGGATACACAGTCCACGCCGCGCTTCAGAGCCATGGGGAATCCAAGTTGGGTTCGGTTTCTTGTGAGAATAACAAGAGGCTGAAGATTTTCCATTTAGACCCATTTGATTACCAGAGCATAGTTGATGCTTTGAAGGGCTGTTCTGGCTTGTTCTACACTTTTGAACCTCCACAAGATCAGCCCGACTATGATGAATATATGGCAGAAGTAGAGGTGAGAGCTGCACACAACGTTCTAGAAGCATGTGCCCGAACGGAAACCATAGACAAGGTTGTGTTCACATCATCAGTCACCGCCGTCGTTTGGAGCAGTAACAATAAATCGACGGTCGCTGCTACGCCGGAGTTGGACGAACGGCACTGGACTGACGTCAACTTGTGTCGCAAGTTCAAGCTATGGCATGCCCTATCAAAAACCCTAGCAGAGAAGACAGCGTGGGCGTTGGCAATGGATAGGAGCTTGAATATGGTGTCTATGAATGCAGGGTTGATGATAAATCCCGACTTGTCCATCAGCAACCCGTACTTGAAGGGAGCGGCGGAGATGTATGAAGACGGGCTGCTCGTGACCGTCGATATAAATTTCTTAGTGGATGCCCATATTTGCGTCTTCGAAGACATCACAGCCTACGGTCGATACTTATGTTTCAACAAAATCATCACTCAGTCGGGAGACGCTGTTGACCTTGCCCGGAAGTTGACCCCTTCTGCCCCGGCCTCCTTGCCCCAAAGCAAGGACCAGGATATGAGAATCATCCAAGAGAAAATAAGCAACAAGAAGTTGAATAAGTTGATGGTGGGTTTCAAGAGTAAATCTCAAATGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

316

Amino Acids

34.94

Weight (kDa)

5.8

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 18 - 141 2.1e-07 NmrA-like family
3Beta_HSD PF01073 23 - 153 1.8e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 24 - 201 1.5e-07 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 26 - 141 9.8e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 41
AccB7I CCANNNNNTGG 1 cut(s) 112
AccBSI CCGCTC 1 cut(s) 659
AccII CGCG 1 cut(s) 143
AciI CCGC 5 cut(s) 9, 141, 417, 659, 662
AclI AACGTT 1 cut(s) 354
AcsI RAATTY 1 cut(s) 703
AcuI CTGAAG 2 cut(s) 131, 224
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 3 cut(s) 112, 426, 856
AgsI TTSAA 8 cut(s) 256, 287, 514, 586, 652, 772, 907, 928
AjnI CCWGG 2 cut(s) 111, 861
AluBI AGCT 5 cut(s) 14, 51, 344, 517, 582
AluI AGCT 5 cut(s) 14, 51, 344, 517, 582
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 1 cut(s) 789
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 2 cut(s) 82, 837
ApeKI GCWGC 4 cut(s) 11, 344, 455, 682
ApoI RAATTY 1 cut(s) 703
Asp700I GAANNNNTTC 2 cut(s) 23, 209
AspLEI GCGC 2 cut(s) 111, 145
AspS9I GGNCC 1 cut(s) 859
AsuC2I CCSGG 2 cut(s) 814, 836
AsuHPI GGTGA 2 cut(s) 349, 406
AsuII TTCGAA 2 cut(s) 17, 735
AvaII GGWCC 1 cut(s) 859
BaeGI GKGCMC 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 751, 784
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 53
BauI CACGAG 2 cut(s) 46, 686
BbsI GAAGAC 4 cut(s) 557, 681, 724, 744
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 4 cut(s) 23, 331, 442, 669
BccI CCATC 2 cut(s) 641, 910
BceAI ACGGC 3 cut(s) 55, 404, 494
BciT130I CCWGG 2 cut(s) 113, 863
BciVI GTATCC 1 cut(s) 119
BcnI CCSGG 2 cut(s) 814, 836
BcoDI GTCTC 1 cut(s) 789
BfaI CTAG 2 cut(s) 359, 542
BfoI RGCGCY 1 cut(s) 112
BfuI GTATCC 1 cut(s) 119
BglI GCCNNNNNGGC 1 cut(s) 90
BisI GCNGC 6 cut(s) 12, 141, 345, 456, 660, 683
BlsI GCNGC 6 cut(s) 13, 142, 346, 457, 661, 684
Bme1390I CCNGG 4 cut(s) 113, 814, 836, 863
Bme18I GGWCC 1 cut(s) 859
BmgT120I GGNCC 1 cut(s) 859
BmiI GGNNCC 2 cut(s) 6, 43
BmrFI CCNGG 4 cut(s) 113, 814, 836, 863
BmsI GCATC 3 cut(s) 64, 238, 706
BoxI GACNNNNGTC 2 cut(s) 101, 491
BpiI GAAGAC 4 cut(s) 557, 681, 724, 744
BplI GAGNNNNNCTC 1 cut(s) 922
Bpu14I TTCGAA 2 cut(s) 17, 735
BpuMI CCSGG 2 cut(s) 814, 836
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 4 cut(s) 37, 88, 154, 834
BsaXI ACNNNNNCTCC 2 cut(s) 786, 816
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 426, 856
Bse1I ACTGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 576
BseBI CCWGG 2 cut(s) 113, 863
BseDI CCNNGG 4 cut(s) 37, 88, 154, 834
BseGI GGATG 3 cut(s) 79, 721, 876
BseLI CCNNNNNNNGG 3 cut(s) 112, 426, 856
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 1 cut(s) 800
BseNI ACTGG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 4 cut(s) 23, 331, 442, 669
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 2 cut(s) 453, 757
BshFI GGCC 2 cut(s) 84, 839
BshNI GGYRCC 1 cut(s) 41
BsiEI CGRYCG 2 cut(s) 453, 757
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 3 cut(s) 464, 814, 836
BslI CCNNNNNNNGG 3 cut(s) 112, 426, 856
BsmAI GTCTC 1 cut(s) 789
BsmBI CGTCTC 1 cut(s) 789
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 2 cut(s) 84, 839
Bsp119I TTCGAA 2 cut(s) 17, 735
Bsp1286I GDGCHC 2 cut(s) 53, 373
Bsp143I GATC 1 cut(s) 298
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 5 cut(s) 9, 141, 417, 659, 662
BspANI GGCC 2 cut(s) 84, 839
BspCNI CTCAG 1 cut(s) 799
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 2 cut(s) 6, 43
BspT104I TTCGAA 2 cut(s) 17, 735
BspT107I GGYRCC 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 659
BsrDI GCAATG 1 cut(s) 576
BsrI ACTGG 1 cut(s) 488
BssECI CCNNGG 4 cut(s) 37, 88, 154, 834
BssMI GATC 1 cut(s) 298
BssNI GRCGYC 1 cut(s) 492
BssSI CACGAG 2 cut(s) 46, 686
BssT1I CCWWGG 1 cut(s) 154
Bst2BI CACGAG 2 cut(s) 46, 686
Bst2UI CCWGG 2 cut(s) 113, 863
Bst4CI ACNGT 5 cut(s) 61, 133, 451, 694, 755
BstACI GRCGYC 1 cut(s) 492
BstBI TTCGAA 2 cut(s) 17, 735
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 2 cut(s) 709, 786
BstDSI CCRYGG 2 cut(s) 37, 154
BstF5I GGATG 3 cut(s) 79, 721, 876
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 2 cut(s) 111, 145
BstKTI GATC 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 789
BstMBI GATC 1 cut(s) 298
BstMCI CGRYCG 2 cut(s) 453, 757
BstMWI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
BstNI CCWGG 2 cut(s) 113, 863
BstNSI RCATGY 2 cut(s) 369, 527
BstPAI GACNNNNGTC 2 cut(s) 101, 491
BstSCI CCNGG 4 cut(s) 111, 812, 834, 861
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 4 cut(s) 23, 331, 442, 669
BstV2I GAAGAC 4 cut(s) 557, 681, 724, 744
BsuI GTATCC 1 cut(s) 119
BsuRI GGCC 2 cut(s) 84, 839
BtgI CCRYGG 2 cut(s) 37, 154
BtsCI GGATG 3 cut(s) 79, 721, 876
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 1 cut(s) 525
CaiI CAGNNNCTG 1 cut(s) 112
CfoI GCGC 2 cut(s) 111, 145
Cfr13I GGNCC 1 cut(s) 859
CseI GACGC 3 cut(s) 66, 718, 806
Csp6I GTAC 1 cut(s) 646
CviAII CATG 3 cut(s) 155, 366, 524
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 2 cut(s) 709, 786
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
EciI GGCGGA 1 cut(s) 677
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 859
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 131, 224
EcoICRI GAGCTC 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 111, 861
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 789
FaeI CATG 3 cut(s) 158, 369, 527
FatI CATG 3 cut(s) 154, 365, 523
FauI CCCGC 1 cut(s) 16
Fnu4HI GCNGC 6 cut(s) 12, 141, 345, 456, 660, 683
FokI GGATG 3 cut(s) 86, 728, 863
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 6 cut(s) 12, 141, 345, 456, 660, 683
FspBI CTAG 2 cut(s) 359, 542
GlaI GCGC 2 cut(s) 110, 144
GluI GCNGC 6 cut(s) 12, 141, 345, 456, 660, 683
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 2 cut(s) 84, 839
HapII CCGG 3 cut(s) 464, 814, 836
HgaI GACGC 3 cut(s) 66, 718, 806
HhaI GCGC 2 cut(s) 111, 145
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 3 cut(s) 158, 369, 527
Hin6I GCGC 2 cut(s) 109, 143
HinP1I GCGC 2 cut(s) 109, 143
HincII GTYRAC 3 cut(s) 496, 805, 822
HindII GTYRAC 3 cut(s) 496, 805, 822
HinfI GANTC 3 cut(s) 19, 161, 873
HpaII CCGG 3 cut(s) 464, 814, 836
HphI GGTGA 2 cut(s) 349, 406
Hpy166II GTNNAC 5 cut(s) 136, 402, 496, 805, 822
Hpy188I TCNGA 2 cut(s) 120, 150
Hpy188III TCNNGA 5 cut(s) 359, 623, 688, 792, 928
Hpy8I GTNNAC 5 cut(s) 136, 402, 496, 805, 822
Hpy99I CGWCG 4 cut(s) 32, 425, 451, 698
HpyAV CCTTC 3 cut(s) 250, 646, 837
HpyCH4III ACNGT 5 cut(s) 61, 133, 451, 694, 755
HpyCH4IV ACGT 2 cut(s) 354, 492
HpyCH4V TGCA 2 cut(s) 347, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
HpyF3I CTNAG 2 cut(s) 709, 786
HpySE526I ACGT 2 cut(s) 354, 492
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 3 cut(s) 158, 369, 527
HspAI GCGC 2 cut(s) 109, 143
Kzo9I GATC 1 cut(s) 298
LmnI GCTCC 4 cut(s) 10, 429, 579, 656
Lsp1109I GCAGC 4 cut(s) 23, 331, 442, 669
LweI GCATC 3 cut(s) 64, 238, 706
MaeI CTAG 2 cut(s) 359, 542
MaeII ACGT 2 cut(s) 354, 492
MaeIII GTNAC 3 cut(s) 412, 434, 688
MalI GATC 1 cut(s) 300
MbiI CCGCTC 1 cut(s) 659
MboI GATC 1 cut(s) 298
MboII GAAGA 5 cut(s) 217, 562, 686, 724, 749
MhlI GDGCHC 2 cut(s) 53, 373
MluCI AATT 1 cut(s) 703
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 6 cut(s) 98, 110, 192, 300, 328, 850
MroXI GAANNNNTTC 2 cut(s) 23, 209
MspI CCGG 3 cut(s) 464, 814, 836
MspR9I CCNGG 4 cut(s) 113, 814, 836, 863
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 2 cut(s) 113, 863
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
NciI CCSGG 2 cut(s) 814, 836
NcoI CCATGG 1 cut(s) 154
NdeII GATC 1 cut(s) 298
NlaIII CATG 3 cut(s) 158, 369, 527
NlaIV GGNNCC 2 cut(s) 6, 43
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 412, 688
NspI RCATGY 2 cut(s) 369, 527
NspV TTCGAA 2 cut(s) 17, 735
PaeI GCATGC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 2 cut(s) 684, 693
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 2 cut(s) 23, 209
PfeI GAWTC 3 cut(s) 19, 161, 873
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 6 cut(s) 13, 142, 346, 457, 661, 684
PshAI GACNNNNGTC 2 cut(s) 101, 491
Psp124BI GAGCTC 1 cut(s) 53
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 111, 861
PspGI CCWGG 2 cut(s) 111, 861
PspN4I GGNNCC 2 cut(s) 6, 43
PspPI GGNCC 1 cut(s) 859
PstNI CAGNNNCTG 1 cut(s) 112
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
SacI GAGCTC 1 cut(s) 53
SatI GCNGC 6 cut(s) 12, 141, 345, 456, 660, 683
Sau3AI GATC 1 cut(s) 298
Sau96I GGNCC 1 cut(s) 859
ScrFI CCNGG 4 cut(s) 113, 814, 836, 863
SduI GDGCHC 2 cut(s) 53, 373
SfaNI GCATC 3 cut(s) 64, 238, 706
SfuI TTCGAA 2 cut(s) 17, 735
SinI GGWCC 1 cut(s) 859
SphI GCATGC 1 cut(s) 527
Sse9I AATT 1 cut(s) 703
SsiI CCGC 5 cut(s) 9, 141, 417, 659, 662
SspMI CTAG 2 cut(s) 359, 542
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 4 cut(s) 111, 812, 834, 861
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 5 cut(s) 61, 133, 451, 694, 755
TaiI ACGT 2 cut(s) 357, 495
TaqI TCGA 8 cut(s) 17, 27, 53, 95, 446, 696, 735, 757
TasI AATT 1 cut(s) 703
TauI GCSGC 2 cut(s) 143, 662
TfiI GAWTC 3 cut(s) 19, 161, 873
TscAI CASTG 1 cut(s) 488
TseFI GTSAC 2 cut(s) 412, 688
TseI GCWGC 4 cut(s) 11, 344, 455, 682
Tsp45I GTSAC 2 cut(s) 412, 688
TspDTI ATGAA 3 cut(s) 330, 614, 687
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 488
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 859
XapI RAATTY 1 cut(s) 703
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 2 cut(s) 369, 527
XmnI GAANNNNTTC 2 cut(s) 23, 209
XspI CTAG 2 cut(s) 359, 542
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.