Rw1G031120

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001892
Physical Location & Seq
Reverse (-)
59205603 .. 59208985
3383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031120.1

Sequence Viewer

Length: 906 bp
ATGGCTCCCGCAGCTTCGAATCCTTTCGACGATCACCACGGCACCACGAGCTCGACCACAGTCTGCGTAATGGATGCGTCAGGCCACCTCGGCTCGACCCTCGTCCAGCGCCTGGTTCAGACGGGATACACAGTCCACGCCGCGCTTCAGAGCCATGGGGAATCCAAGTTGGGTTCGGTTTCTTGTGAGAATAACAAGAGGCTGAAGATTTTCCATTTAGACCCATTTGATTACCAGAGCATAGTTGATGCTTTGAAGGGCTGTTCTGGCTTGTTCTACACTTTTGAACCTCCACAAGATCAGCCCGACTATGATGAATATATGGCAGAAGTAGAGGTGAGAGCTGCACACAACGTTCTAGAAGCATGTGCCCGAACGGAAACCATAGACAAGGTTGTGTTCACATCATCAGTCACCGCCGTCGTTTGGAGCAGTAACAATAAATCGACGCTATGGCATGCCCTATCAAAAACCCTAGCAGAGAAGACAGCGTGGGCGTTGGCAATGGATAGGAGCTTGAATATGGTGTCTATGAATGCAGGGTTGATGATAAATCCCGACTTGTCCATCAGCAACCCGTACTTGAAGGGAGCGGCGGAGATGTATGAAGACGGGCTGCTCGTGACCGTCGATATAAATTTCTTAGTGGATGCCCATATTTGCGTCTTCGAAGACATCACAGCCTACGGTCGATACTTATGTTTCAACAAAATCATCACTCAGTCGGGAGACGCTGTTGACCTTGCCCGGAAGTTGACCCCTTCTGCCCCGGCCTCCTTGCCCCAAAGCAAGGACCAGGATATGAGAATCATCCAAGAGAGAATAAGGGCGACCAAACTGGGCCTAAGCAACAAGAAGTTGAACAAGTTGATGGTGGGTTTCAAGAGTAAATCTCAAATGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

301

Amino Acids

33.08

Weight (kDa)

5.98

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 19 - 157 1e-07 NmrA-like family
3Beta_HSD PF01073 23 - 143 6.3e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 24 - 181 3.1e-08 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 26 - 171 1.1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccB7I CCANNNNNTGG 1 cut(s) 112
AccBSI CCGCTC 1 cut(s) 593
AccII CGCG 1 cut(s) 143
AciI CCGC 5 cut(s) 9, 141, 417, 593, 596
AclI AACGTT 1 cut(s) 354
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 2 cut(s) 131, 224
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 3 cut(s) 112, 426, 790
AgsI TTSAA 7 cut(s) 256, 287, 520, 586, 706, 862, 883
AjnI CCWGG 2 cut(s) 111, 795
AluBI AGCT 4 cut(s) 14, 51, 344, 516
AluI AGCT 4 cut(s) 14, 51, 344, 516
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 1 cut(s) 723
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 3 cut(s) 82, 771, 841
ApeKI GCWGC 3 cut(s) 11, 344, 616
ApoI RAATTY 1 cut(s) 637
Asp700I GAANNNNTTC 2 cut(s) 23, 209
AspLEI GCGC 2 cut(s) 111, 145
AspS9I GGNCC 2 cut(s) 793, 841
AsuC2I CCSGG 2 cut(s) 748, 770
AsuHPI GGTGA 3 cut(s) 26, 349, 406
AsuII TTCGAA 2 cut(s) 17, 669
AvaII GGWCC 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 685, 718
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 53
BauI CACGAG 2 cut(s) 46, 620
BbsI GAAGAC 4 cut(s) 491, 615, 658, 678
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 23, 331, 603
BccI CCATC 2 cut(s) 575, 865
BceAI ACGGC 2 cut(s) 55, 404
BciT130I CCWGG 2 cut(s) 113, 797
BciVI GTATCC 1 cut(s) 119
BcnI CCSGG 2 cut(s) 748, 770
BcoDI GTCTC 1 cut(s) 723
BfaI CTAG 2 cut(s) 359, 476
BfoI RGCGCY 1 cut(s) 112
BfuI GTATCC 1 cut(s) 119
BglI GCCNNNNNGGC 1 cut(s) 90
BisI GCNGC 5 cut(s) 12, 141, 345, 594, 617
BlsI GCNGC 5 cut(s) 13, 142, 346, 595, 618
Bme1390I CCNGG 4 cut(s) 113, 748, 770, 797
Bme18I GGWCC 1 cut(s) 793
BmgT120I GGNCC 2 cut(s) 793, 841
BmiI GGNNCC 2 cut(s) 6, 43
BmrFI CCNGG 4 cut(s) 113, 748, 770, 797
BmrI ACTGGG 1 cut(s) 848
BmsI GCATC 3 cut(s) 64, 238, 640
BmuI ACTGGG 1 cut(s) 848
BoxI GACNNNNGTC 2 cut(s) 59, 101
BpiI GAAGAC 4 cut(s) 491, 615, 658, 678
BplI GAGNNNNNCTC 1 cut(s) 877
Bpu10I CCTNAGC 1 cut(s) 845
Bpu14I TTCGAA 2 cut(s) 17, 669
BpuMI CCSGG 2 cut(s) 748, 770
BsaJI CCNNGG 4 cut(s) 37, 88, 154, 768
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 426, 790
Bse1I ACTGG 1 cut(s) 843
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 2 cut(s) 113, 797
BseDI CCNNGG 4 cut(s) 37, 88, 154, 768
BseGI GGATG 3 cut(s) 79, 655, 810
BseLI CCNNNNNNNGG 3 cut(s) 112, 426, 790
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 1 cut(s) 734
BseNI ACTGG 1 cut(s) 843
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 3 cut(s) 23, 331, 603
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 691
BshFI GGCC 3 cut(s) 84, 773, 843
BshNI GGYRCC 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 691
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 2 cut(s) 748, 770
BslI CCNNNNNNNGG 3 cut(s) 112, 426, 790
BsmAI GTCTC 1 cut(s) 723
BsmBI CGTCTC 1 cut(s) 723
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 3 cut(s) 84, 773, 843
Bsp119I TTCGAA 2 cut(s) 17, 669
Bsp1286I GDGCHC 2 cut(s) 53, 373
Bsp143I GATC 2 cut(s) 31, 298
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 5 cut(s) 9, 141, 417, 593, 596
BspANI GGCC 3 cut(s) 84, 773, 843
BspCNI CTCAG 1 cut(s) 733
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 2 cut(s) 6, 43
BspT104I TTCGAA 2 cut(s) 17, 669
BspT107I GGYRCC 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 593
BsrDI GCAATG 1 cut(s) 510
BsrI ACTGG 1 cut(s) 843
BssECI CCNNGG 4 cut(s) 37, 88, 154, 768
BssMI GATC 2 cut(s) 31, 298
BssSI CACGAG 2 cut(s) 46, 620
BssT1I CCWWGG 1 cut(s) 154
Bst2BI CACGAG 2 cut(s) 46, 620
Bst2UI CCWGG 2 cut(s) 113, 797
Bst4CI ACNGT 4 cut(s) 61, 133, 628, 689
BstBI TTCGAA 2 cut(s) 17, 669
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 3 cut(s) 643, 720, 845
BstDSI CCRYGG 2 cut(s) 37, 154
BstF5I GGATG 3 cut(s) 79, 655, 810
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 2 cut(s) 111, 145
BstKTI GATC 2 cut(s) 34, 301
BstMAI GTCTC 1 cut(s) 723
BstMBI GATC 2 cut(s) 31, 298
BstMCI CGRYCG 1 cut(s) 691
BstMWI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
BstNI CCWGG 2 cut(s) 113, 797
BstNSI RCATGY 2 cut(s) 369, 461
BstPAI GACNNNNGTC 2 cut(s) 59, 101
BstSCI CCNGG 4 cut(s) 111, 746, 768, 795
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 3 cut(s) 23, 331, 603
BstV2I GAAGAC 4 cut(s) 491, 615, 658, 678
BsuI GTATCC 1 cut(s) 119
BsuRI GGCC 3 cut(s) 84, 773, 843
BtgI CCRYGG 2 cut(s) 37, 154
BtsCI GGATG 3 cut(s) 79, 655, 810
Cac8I GCNNGC 1 cut(s) 459
CaiI CAGNNNCTG 1 cut(s) 112
CfoI GCGC 2 cut(s) 111, 145
Cfr13I GGNCC 2 cut(s) 793, 841
CseI GACGC 4 cut(s) 66, 457, 652, 740
Csp6I GTAC 1 cut(s) 580
CviAII CATG 3 cut(s) 155, 366, 458
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 3 cut(s) 643, 720, 845
DpnI GATC 2 cut(s) 33, 300
DpnII GATC 2 cut(s) 31, 298
EciI GGCGGA 1 cut(s) 611
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 793
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 131, 224
EcoICRI GAGCTC 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 111, 795
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 723
FaeI CATG 3 cut(s) 158, 369, 461
FatI CATG 3 cut(s) 154, 365, 457
FauI CCCGC 1 cut(s) 16
Fnu4HI GCNGC 5 cut(s) 12, 141, 345, 594, 617
FokI GGATG 3 cut(s) 86, 662, 797
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 5 cut(s) 12, 141, 345, 594, 617
FspBI CTAG 2 cut(s) 359, 476
GlaI GCGC 2 cut(s) 110, 144
GluI GCNGC 5 cut(s) 12, 141, 345, 594, 617
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 3 cut(s) 84, 773, 843
HapII CCGG 2 cut(s) 748, 770
HgaI GACGC 4 cut(s) 66, 457, 652, 740
HhaI GCGC 2 cut(s) 111, 145
Hin1II CATG 3 cut(s) 158, 369, 461
Hin6I GCGC 2 cut(s) 109, 143
HinP1I GCGC 2 cut(s) 109, 143
HincII GTYRAC 2 cut(s) 739, 756
HindII GTYRAC 2 cut(s) 739, 756
HinfI GANTC 3 cut(s) 19, 161, 807
HpaII CCGG 2 cut(s) 748, 770
HphI GGTGA 3 cut(s) 26, 349, 406
Hpy166II GTNNAC 4 cut(s) 136, 402, 739, 756
Hpy188I TCNGA 2 cut(s) 120, 150
Hpy188III TCNNGA 5 cut(s) 359, 557, 622, 726, 883
Hpy8I GTNNAC 4 cut(s) 136, 402, 739, 756
Hpy99I CGWCG 4 cut(s) 32, 425, 451, 632
HpyAV CCTTC 3 cut(s) 250, 580, 771
HpyCH4III ACNGT 4 cut(s) 61, 133, 628, 689
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 2 cut(s) 347, 539
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
HpyF3I CTNAG 3 cut(s) 643, 720, 845
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 3 cut(s) 158, 369, 461
HspAI GCGC 2 cut(s) 109, 143
Kzo9I GATC 2 cut(s) 31, 298
LmnI GCTCC 4 cut(s) 10, 429, 513, 590
Lsp1109I GCAGC 3 cut(s) 23, 331, 603
LweI GCATC 3 cut(s) 64, 238, 640
MaeI CTAG 2 cut(s) 359, 476
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 3 cut(s) 412, 434, 622
MalI GATC 2 cut(s) 33, 300
MbiI CCGCTC 1 cut(s) 593
MboI GATC 2 cut(s) 31, 298
MboII GAAGA 5 cut(s) 217, 496, 620, 658, 683
MhlI GDGCHC 2 cut(s) 53, 373
MluCI AATT 1 cut(s) 637
MnlI CCTC 6 cut(s) 98, 110, 192, 300, 328, 784
MroXI GAANNNNTTC 2 cut(s) 23, 209
MspI CCGG 2 cut(s) 748, 770
MspR9I CCNGG 4 cut(s) 113, 748, 770, 797
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 113, 797
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 4 cut(s) 11, 48, 90, 267
NciI CCSGG 2 cut(s) 748, 770
NcoI CCATGG 1 cut(s) 154
NdeII GATC 2 cut(s) 31, 298
NlaIII CATG 3 cut(s) 158, 369, 461
NlaIV GGNNCC 2 cut(s) 6, 43
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 412, 622
NspI RCATGY 2 cut(s) 369, 461
NspV TTCGAA 2 cut(s) 17, 669
PaeI GCATGC 1 cut(s) 461
PcsI WCGNNNNNNNCGW 2 cut(s) 618, 627
PctI GAATGC 1 cut(s) 541
PdmI GAANNNNTTC 2 cut(s) 23, 209
PfeI GAWTC 3 cut(s) 19, 161, 807
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 5 cut(s) 13, 142, 346, 595, 618
PshAI GACNNNNGTC 2 cut(s) 59, 101
Psp124BI GAGCTC 1 cut(s) 53
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 111, 795
PspGI CCWGG 2 cut(s) 111, 795
PspN4I GGNNCC 2 cut(s) 6, 43
PspPI GGNCC 2 cut(s) 793, 841
PstNI CAGNNNCTG 1 cut(s) 112
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
SacI GAGCTC 1 cut(s) 53
SatI GCNGC 5 cut(s) 12, 141, 345, 594, 617
Sau3AI GATC 2 cut(s) 31, 298
Sau96I GGNCC 2 cut(s) 793, 841
ScrFI CCNGG 4 cut(s) 113, 748, 770, 797
SduI GDGCHC 2 cut(s) 53, 373
SfaNI GCATC 3 cut(s) 64, 238, 640
SfuI TTCGAA 2 cut(s) 17, 669
SinI GGWCC 1 cut(s) 793
SphI GCATGC 1 cut(s) 461
Sse9I AATT 1 cut(s) 637
SsiI CCGC 5 cut(s) 9, 141, 417, 593, 596
SspMI CTAG 2 cut(s) 359, 476
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 4 cut(s) 111, 746, 768, 795
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 4 cut(s) 61, 133, 628, 689
TaiI ACGT 1 cut(s) 357
TaqI TCGA 8 cut(s) 17, 27, 53, 95, 446, 630, 669, 691
TasI AATT 1 cut(s) 637
TauI GCSGC 2 cut(s) 143, 596
TfiI GAWTC 3 cut(s) 19, 161, 807
TseFI GTSAC 2 cut(s) 412, 622
TseI GCWGC 3 cut(s) 11, 344, 616
Tsp45I GTSAC 2 cut(s) 412, 622
TspDTI ATGAA 3 cut(s) 330, 548, 621
TspGWI ACGGA 1 cut(s) 392
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 793
XapI RAATTY 1 cut(s) 637
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 2 cut(s) 369, 461
XmnI GAANNNNTTC 2 cut(s) 23, 209
XspI CTAG 2 cut(s) 359, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.