Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001892

Physical Location & Seq

Genomic Coordinates Forward (+)

36501879 .. 36504229

Gene Structure 2351 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1310200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 999 bp

ATGGCTCTTGCAGCAGATGCTATTTGTACTTGTGATGATGATGGTGCTGATGAAATTAGTCCATGTTCAGGGACGACCACAGTCTGCGTCATGGATGCGTCCGGCCGCCTAGGCTCCACTCTCGTCCATCGCCTCTTGCACAGAGGCTATGCAGTCCATGCTGCACTCCAAACCCATCACAAGTGTTTTGATGACGAATTGGTAAAGAAGAAGAAAGTGAGCGTTTTCCGTTCAGACCCCTTTGACTACCACAGCATTATGGTCGCTTTGGAAGGATGCTCTGCCCTTTTCTACTCCTTCGAGCCTCCTTCAGACCACCCCACTTATGATGAATTTATGGCAGAGGCAGAGGTCAGAGCAGCTCATAACGTACTGGAAGCTTGTGCCCAAACTGATACCATACACAAAGTAGTCTTTACATCCTCTGTAACAGCTGTGATTTGGAGGGACGACCGTACAACGTCGCCGTCCTCCTCCCATGAGATTGATGAGAGAAACTGGAGCGATGTCAATTTTTGTAAAAAATTCAAGCTGTGGCATGCCCTCTCGAAGACCCTAGCCGAGAAGACAGCATGGGCTCTAGCAATGGACCGAGGACTGAACATGGTGTCCGTCAATGGCGGGCTGTTGATGAGCCCCGATCTCACCATCACTAATCCGTATCTAAAAGGAACGGCTGAGATGTACCAAGATGGGCTGTTGGTGACCGTTGATCTCAAGTTCCTTGTTGACGCACACATCTCCGTCCTTGAAGAGGCGTCATCCTACGGAAGATACGTATGCTTCGACAGAGTCATCAATTGCTACAAAGATGCCGTTGGGCTCGCCCGCATGCTTTTGCCGCCTTCTCAAACTTCATTTCTCCAAACGCAAACCCAACCCCAAACCCCAAGTTTCGAGGAGACGGAGACAATGGTGGTGTACCAACAGAAGATAAGCAACGAGAAACTAAACAAACTCATGGTCGACTTTGACACCACCCTTCAACTCAGCCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0009058 GO:0009718 GO:0009812 GO:0009813 GO:0012505 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0019898 GO:0031090 GO:0031312 GO:0031984 GO:0042175 GO:0042406 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045552 GO:0046148 GO:0046283 GO:0055114 GO:0071704 GO:0098827 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

333

Amino Acids

37.01

Weight (kDa)

5.25

Isoelectric Point (pI)

35.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	25 - 144	7.1e-07	NmrA-like family
3Beta_HSD	PF01073	30 - 148	4.8e-08	3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase	PF01370	32 - 213	3e-06	NAD dependent epimerase/dehydratase family
NAD_binding_10	PF13460	33 - 145	1.5e-07	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23910 AT2G23910 AT2G23910 AT2G23910 AT4G30470
fragaria_vesca	FvH4_5g22420 FvH4_5g22420 FvH4_5g22420
malus_domestica	MD00G1072500.v1.1 MD01G1237600.v1.1 MD06G1036600.v1.1 MD07G1310200.v1.1
prunus_persica	Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.5G032800_v2.0.a1
pyrus_communis	pycom01g24530 pycom06g03000 pycom13g28670
rosa_chinensis	RchiOBHm_Chr4g0405161 RchiOBHm_Chr7g0210161
rosa_laevigata	RLG00000003059 RLG00000008867
rosa_multiflora	Rmu_sc0005438.1_g000001 Rmu_sc0006908.1_g000015 Rmu_ssc0000255.1_g000034
rosa_roxburghii	Rroxscaffold_3G00248570 Rroxscaffold_5G00349400
rosa_rugosa	Rorug04G0054400 Rorug04G0054500 Rorug04G0054600 Rorug07G0119000
rosa_samantha	Rh1AG349400 Rh4AG129400 Rh4BG123100 Rh4CG136700 Rh4DG122900 Rh7AG252500 Rh7BG247600 Rh7CG269500 Rh7DG260100
rosa_wichuraiana	Rw0G002460 Rw1G031120 Rw4G010450 Rw7G021460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	966
AciI	CCGC	4 cut(s)	106, 621, 829, 842
AcoI	YGGCCR	1 cut(s)	103
AcsI	RAATTY	2 cut(s)	332, 524
AcuI	CTGAAG	1 cut(s)	294
AcyI	GRCGYC	1 cut(s)	758
AfaI	GTAC	5 cut(s)	28, 372, 457, 686, 923
AfiI	CCNNNNNNNGG	2 cut(s)	68, 754
AgsI	TTSAA	3 cut(s)	529, 752, 986
AloI	GAACNNNNNNTCC	2 cut(s)	593, 625
AluBI	AGCT	4 cut(s)	362, 380, 434, 532
AluI	AGCT	4 cut(s)	362, 380, 434, 532
Alw26I	GTCTC	2 cut(s)	896, 902
AoxI	GGCC	1 cut(s)	103
ApeKI	GCWGC	3 cut(s)	11, 161, 359
ApoI	RAATTY	2 cut(s)	332, 524
ArsI	GACNNNNNNTTYG	2 cut(s)	948, 980
AspA2I	CCTAGG	1 cut(s)	109
AspS9I	GGNCC	1 cut(s)	589
AsuHPI	GGTGA	2 cut(s)	637, 715
AvaII	GGWCC	1 cut(s)	589
AvrII	CCTAGG	1 cut(s)	109
BaeGI	GKGCMC	1 cut(s)	388
BanII	GRGCYC	3 cut(s)	580, 638, 825
BbsI	GAAGAC	2 cut(s)	557, 572
BbvI	GCAGC	3 cut(s)	23, 148, 371
BccI	CCATC	5 cut(s)	35, 135, 183, 656, 686
BceAI	ACGGC	3 cut(s)	451, 690, 800
BcgI	CGANNNNNNTGC	2 cut(s)	244, 278
BcoDI	GTCTC	2 cut(s)	896, 902
BfaI	CTAG	3 cut(s)	110, 557, 581
BglI	GCCNNNNNGGC	1 cut(s)	111
BisI	GCNGC	5 cut(s)	12, 106, 162, 360, 842
BlnI	CCTAGG	1 cut(s)	109
BlsI	GCNGC	5 cut(s)	13, 107, 163, 361, 843
Bme18I	GGWCC	1 cut(s)	589
BmgT120I	GGNCC	1 cut(s)	589
BmiI	GGNNCC	1 cut(s)	115
BmsI	GCATC	4 cut(s)	7, 85, 266, 802
BoxI	GACNNNNGTC	1 cut(s)	80
BpiI	GAAGAC	2 cut(s)	557, 572
BpmI	CTGGAG	1 cut(s)	520
BpuEI	CTTGAG	1 cut(s)	701
BsaAI	YACGTR	1 cut(s)	778
BsaHI	GRCGYC	1 cut(s)	758
BsaJI	CCNNGG	2 cut(s)	109, 592
Bsc4I	CCNNNNNNNGG	2 cut(s)	68, 754
Bse1I	ACTGG	2 cut(s)	378, 503
Bse3DI	GCAATG	1 cut(s)	591
BseDI	CCNNGG	2 cut(s)	109, 592
BseGI	GGATG	4 cut(s)	100, 281, 419, 761
BseLI	CCNNNNNNNGG	2 cut(s)	68, 754
BseMI	GCAATG	1 cut(s)	591
BseMII	CTCAG	1 cut(s)	669
BseNI	ACTGG	2 cut(s)	378, 503
BseRI	GAGGAG	2 cut(s)	463, 914
BseSI	GKGCMC	1 cut(s)	388
BseX3I	CGGCCG	1 cut(s)	103
BseXI	GCAGC	3 cut(s)	23, 148, 371
BsgI	GTGCAG	1 cut(s)	147
Bsh1285I	CGRYCG	2 cut(s)	106, 454
BshFI	GGCC	1 cut(s)	105
BsiEI	CGRYCG	2 cut(s)	106, 454
BsiSI	CCGG	1 cut(s)	102
BslFI	GGGAC	2 cut(s)	85, 461
BslI	CCNNNNNNNGG	2 cut(s)	68, 754
BsmAI	GTCTC	2 cut(s)	896, 902
BsmBI	CGTCTC	1 cut(s)	896
BsmFI	GGGAC	2 cut(s)	85, 461
BsnI	GGCC	1 cut(s)	105
Bsp1286I	GDGCHC	4 cut(s)	388, 580, 638, 825
Bsp143I	GATC	2 cut(s)	640, 712
BspACI	CCGC	4 cut(s)	106, 621, 829, 842
BspANI	GGCC	1 cut(s)	105
BspCNI	CTCAG	1 cut(s)	670
BspLI	GGNNCC	1 cut(s)	115
BsrDI	GCAATG	1 cut(s)	591
BsrI	ACTGG	2 cut(s)	378, 503
BssECI	CCNNGG	2 cut(s)	109, 592
BssMI	GATC	2 cut(s)	640, 712
BssNI	GRCGYC	1 cut(s)	758
BssT1I	CCWWGG	1 cut(s)	109
Bst4CI	ACNGT	3 cut(s)	82, 455, 709
Bst6I	CTCTTC	1 cut(s)	747
BstACI	GRCGYC	1 cut(s)	758
BstAPI	GCANNNNNTGC	2 cut(s)	17, 158
BstBAI	YACGTR	1 cut(s)	778
BstC8I	GCNNGC	5 cut(s)	540, 623, 825, 829, 833
BstDEI	CTNAG	2 cut(s)	678, 989
BstEII	GGTNACC	1 cut(s)	703
BstENI	CCTNNNNNAGG	1 cut(s)	752
BstF5I	GGATG	4 cut(s)	100, 281, 419, 761
BstKTI	GATC	2 cut(s)	643, 715
BstMAI	GTCTC	2 cut(s)	896, 902
BstMBI	GATC	2 cut(s)	640, 712
BstMCI	CGRYCG	2 cut(s)	106, 454
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 17, 111, 158, 841
BstNSI	RCATGY	2 cut(s)	542, 835
BstPAI	GACNNNNGTC	1 cut(s)	80
BstPI	GGTNACC	1 cut(s)	703
BstSLI	GKGCMC	1 cut(s)	388
BstSNI	TACGTA	1 cut(s)	778
BstV1I	GCAGC	3 cut(s)	23, 148, 371
BstV2I	GAAGAC	2 cut(s)	557, 572
BstZI	CGGCCG	1 cut(s)	103
BsuRI	GGCC	1 cut(s)	105
BtgZI	GCGATG	2 cut(s)	113, 519
BtsCI	GGATG	4 cut(s)	100, 281, 419, 761
Cac8I	GCNNGC	5 cut(s)	540, 623, 825, 829, 833
Cfr13I	GGNCC	1 cut(s)	589
CseI	GACGC	4 cut(s)	76, 87, 740, 747
Csp6I	GTAC	5 cut(s)	27, 371, 456, 685, 922
CviAII	CATG	9 cut(s)	63, 91, 158, 479, 539, 573, 604, 832, 961
CviQI	GTAC	5 cut(s)	27, 371, 456, 685, 922
DdeI	CTNAG	2 cut(s)	678, 989
DpnI	GATC	2 cut(s)	642, 714
DpnII	GATC	2 cut(s)	640, 712
EaeI	YGGCCR	1 cut(s)	103
EagI	CGGCCG	1 cut(s)	103
Eam1104I	CTCTTC	1 cut(s)	747
EarI	CTCTTC	1 cut(s)	747
EclXI	CGGCCG	1 cut(s)	103
Eco105I	TACGTA	1 cut(s)	778
Eco130I	CCWWGG	1 cut(s)	109
Eco24I	GRGCYC	3 cut(s)	580, 638, 825
Eco47I	GGWCC	1 cut(s)	589
Eco52I	CGGCCG	1 cut(s)	103
Eco57I	CTGAAG	1 cut(s)	294
Eco91I	GGTNACC	1 cut(s)	703
EcoNI	CCTNNNNNAGG	1 cut(s)	752
EcoO65I	GGTNACC	1 cut(s)	703
EcoT14I	CCWWGG	1 cut(s)	109
EcoT38I	GRGCYC	3 cut(s)	580, 638, 825
ErhI	CCWWGG	1 cut(s)	109
Esp3I	CGTCTC	1 cut(s)	896
FaeI	CATG	9 cut(s)	66, 94, 161, 482, 542, 576, 607, 835, 964
FaqI	GGGAC	2 cut(s)	85, 461
FatI	CATG	9 cut(s)	62, 90, 157, 478, 538, 572, 603, 831, 960
FauI	CCCGC	2 cut(s)	614, 836
FblI	GTMKAC	1 cut(s)	966
Fnu4HI	GCNGC	5 cut(s)	12, 106, 162, 360, 842
FokI	GGATG	4 cut(s)	107, 288, 406, 748
FriOI	GRGCYC	3 cut(s)	580, 638, 825
Fsp4HI	GCNGC	5 cut(s)	12, 106, 162, 360, 842
FspBI	CTAG	3 cut(s)	110, 557, 581
GluI	GCNGC	5 cut(s)	12, 106, 162, 360, 842
GsuI	CTGGAG	1 cut(s)	520
HaeIII	GGCC	1 cut(s)	105
HapII	CCGG	1 cut(s)	102
HgaI	GACGC	4 cut(s)	76, 87, 740, 747
Hin1I	GRCGYC	1 cut(s)	758
Hin1II	CATG	9 cut(s)	66, 94, 161, 482, 542, 576, 607, 835, 964
HincII	GTYRAC	2 cut(s)	730, 967
HindII	GTYRAC	2 cut(s)	730, 967
HindIII	AAGCTT	1 cut(s)	378
HinfI	GANTC	1 cut(s)	792
HpaII	CCGG	1 cut(s)	102
HphI	GGTGA	2 cut(s)	637, 715
Hpy166II	GTNNAC	3 cut(s)	730, 922, 967
Hpy188I	TCNGA	3 cut(s)	235, 313, 356
Hpy188III	TCNNGA	1 cut(s)	547
Hpy8I	GTNNAC	3 cut(s)	730, 922, 967
Hpy99I	CGWCG	1 cut(s)	466
HpyAV	CCTTC	5 cut(s)	266, 307, 318, 855, 992
HpyCH4III	ACNGT	3 cut(s)	82, 455, 709
HpyCH4IV	ACGT	3 cut(s)	369, 461, 777
HpyCH4V	TGCA	4 cut(s)	11, 139, 152, 164
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 17, 111, 158, 841
HpyF3I	CTNAG	2 cut(s)	678, 989
HpySE526I	ACGT	3 cut(s)	369, 461, 777
Hsp92I	GRCGYC	1 cut(s)	758
Hsp92II	CATG	9 cut(s)	66, 94, 161, 482, 542, 576, 607, 835, 964
Kzo9I	GATC	2 cut(s)	640, 712
LmnI	GCTCC	2 cut(s)	119, 501
LpnPI	CCDG	4 cut(s)	54, 115, 359, 484
Lsp1109I	GCAGC	3 cut(s)	23, 148, 371
LweI	GCATC	4 cut(s)	7, 85, 266, 802
MaeI	CTAG	3 cut(s)	110, 557, 581
MaeII	ACGT	3 cut(s)	369, 461, 777
MaeIII	GTNAC	2 cut(s)	427, 703
MalI	GATC	2 cut(s)	642, 714
MboI	GATC	2 cut(s)	640, 712
MboII	GAAGA	7 cut(s)	220, 223, 562, 577, 764, 783, 943
MfeI	CAATTG	1 cut(s)	799
MhlI	GDGCHC	4 cut(s)	388, 580, 638, 825
MluCI	AATT	6 cut(s)	54, 197, 332, 511, 524, 799
MlyI	GAGTC	1 cut(s)	801
MseI	TTAA	1 cut(s)	997
MspA1I	CMGCKG	1 cut(s)	434
MspI	CCGG	1 cut(s)	102
MunI	CAATTG	1 cut(s)	799
MwoI	GCNNNNNNNGC	5 cut(s)	11, 17, 111, 158, 841
NdeII	GATC	2 cut(s)	640, 712
NlaIII	CATG	9 cut(s)	66, 94, 161, 482, 542, 576, 607, 835, 964
NlaIV	GGNNCC	1 cut(s)	115
NmeAIII	GCCGAG	1 cut(s)	586
NmuCI	GTSAC	1 cut(s)	703
NspI	RCATGY	2 cut(s)	542, 835
PaeI	GCATGC	2 cut(s)	542, 835
PcsI	WCGNNNNNNNCGW	2 cut(s)	774, 783
PflFI	GACNNNGTC	1 cut(s)	791
PkrI	GCNGC	5 cut(s)	13, 107, 163, 361, 843
PleI	GAGTC	1 cut(s)	800
PpsI	GAGTC	1 cut(s)	800
Ppu21I	YACGTR	1 cut(s)	778
PshAI	GACNNNNGTC	1 cut(s)	80
PspEI	GGTNACC	1 cut(s)	703
PspN4I	GGNNCC	1 cut(s)	115
PspPI	GGNCC	1 cut(s)	589
PsyI	GACNNNGTC	1 cut(s)	791
PvuII	CAGCTG	1 cut(s)	434
RsaI	GTAC	5 cut(s)	28, 372, 457, 686, 923
RsaNI	GTAC	5 cut(s)	27, 371, 456, 685, 922
SalI	GTCGAC	1 cut(s)	965
SaqAI	TTAA	1 cut(s)	997
SatI	GCNGC	5 cut(s)	12, 106, 162, 360, 842
Sau3AI	GATC	2 cut(s)	640, 712
Sau96I	GGNCC	1 cut(s)	589
SchI	GAGTC	1 cut(s)	801
SduI	GDGCHC	4 cut(s)	388, 580, 638, 825
SetI	ASST	8 cut(s)	354, 364, 372, 382, 436, 464, 534, 780
SfaNI	GCATC	4 cut(s)	7, 85, 266, 802
SinI	GGWCC	1 cut(s)	589
SmlI	CTYRAG	1 cut(s)	716
SmoI	CTYRAG	1 cut(s)	716
SnaBI	TACGTA	1 cut(s)	778
SphI	GCATGC	2 cut(s)	542, 835
Sse9I	AATT	6 cut(s)	54, 197, 332, 511, 524, 799
SsiI	CCGC	4 cut(s)	106, 621, 829, 842
SspMI	CTAG	3 cut(s)	110, 557, 581
StyI	CCWWGG	1 cut(s)	109
TaaI	ACNGT	3 cut(s)	82, 455, 709
TaiI	ACGT	3 cut(s)	372, 464, 780
TaqI	TCGA	5 cut(s)	300, 548, 786, 897, 966
TaqII	GACCGA	1 cut(s)	606
TasI	AATT	6 cut(s)	54, 197, 332, 511, 524, 799
TatI	WGTACW	1 cut(s)	26
TauI	GCSGC	2 cut(s)	108, 844
Tru1I	TTAA	1 cut(s)	997
Tru9I	TTAA	1 cut(s)	997
TseFI	GTSAC	1 cut(s)	703
TseI	GCWGC	3 cut(s)	11, 161, 359
Tsp45I	GTSAC	1 cut(s)	703
TspDTI	ATGAA	3 cut(s)	66, 345, 846
TspGWI	ACGGA	6 cut(s)	218, 601, 648, 733, 783, 920
Tth111I	GACNNNGTC	1 cut(s)	791
VpaK11BI	GGWCC	1 cut(s)	589
XagI	CCTNNNNNAGG	1 cut(s)	752
XapI	RAATTY	2 cut(s)	332, 524
XceI	RCATGY	2 cut(s)	542, 835
XmaJI	CCTAGG	1 cut(s)	109
XmiI	GTMKAC	1 cut(s)	966
XspI	CTAG	3 cut(s)	110, 557, 581

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.