Rroxscaffold_3G00248570

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001892
Physical Location & Seq
Forward (+)
41914583 .. 41917191
2609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248570.1

Sequence Viewer

Length: 1029 bp
ATGTTGCCAGCACCCTTTACAACTTCAAACCCAAAACAAAAGCAAAGCTTAGACCCTCTCTCTCTCTCTCTACCAGAAATGGCTCCCGCAGCTTCGAATCCTTTCGACGACCACCACGGCACCACGAGCTCGACCACAGTCTGCGTAATGGATGCATCGGGACACCTCGGCTCGACCCTCGTCCAGCGCCTGGTTCAGAGGGGCTACACAGTCCACGCCGCGCTCCAGAGCCATGGGGAATCCAAGTTGGGTTCGGTTTCTTGTGAGAATAACAAGAGGCTGAAGATTTTCCATTTAGACCCATTTGATTACCAGAGCATAGTTGATGCTTTGAAGGGCTGTTCTGGCTTGTTCTACACTTTTGAACCTCCACAAGATCAGCCCGACTATGATGAATATATGGCAGAAGTAGAGGTGAGAGCCGCACACAACGTTCTAGAAGCATGTGCCCGAACGGAAACCATAGACAAGGTTGTGTTCACATCATCAGTCACCGCCGTCGTTTGGAGCAGTAACAACAAATCGACGGTCGCTGCTACGCCGGAGTTGGACGAACGGCACTGGACTGACGTCAACTTCTGTCGCAAGTTCAAGCTATGGCATGCCCTATCAAAAACCCTAGCAGAGAAGACAGCGTGGGCGTTGGCAATGGATAGGAGCTTGAATATGGTGTCTATGAATGCAGGGTTGATGATAAATCCCGACTTGTCCATCAGCAACCCGTACTTGAAGGGAGCGGCGGAGATGTATGAAGACGGCGTGCTTGTGACCGTCGATATAAATTTCTTAGTGGATGCCCATATTTGCGTCTTCGAAGACATCACAGCCTACGGTCGATACATATGTTTCAACAAAATCATCACTCTGTCGGAAGACGCTGTTGACCTTGCCCGGAAGTTGACCCCTTCTGCCCCGGCCTCGTTGCCCCAAAGCAAGGACCAGGATATGAGAATCATCCAAGAGAGAATAAGCAGCAAGAAGTTGAATAAGTTGATGGTGGGTTTCAAGAGTAAATCTCAAATGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

342

Amino Acids

37.88

Weight (kDa)

5.81

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 45 - 167 2.2e-07 NmrA-like family
3Beta_HSD PF01073 49 - 179 1.9e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 50 - 227 6.2e-08 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 52 - 167 3.1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 573
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 1 cut(s) 190
AccBSI CCGCTC 1 cut(s) 737
AccII CGCG 1 cut(s) 221
AciI CCGC 6 cut(s) 87, 219, 423, 495, 737, 740
AclI AACGTT 1 cut(s) 432
AcsI RAATTY 1 cut(s) 781
AcuI CTGAAG 1 cut(s) 302
AcyI GRCGYC 1 cut(s) 570
AfaI GTAC 1 cut(s) 725
AfiI CCNNNNNNNGG 3 cut(s) 190, 504, 934
AgsI TTSAA 9 cut(s) 27, 334, 365, 592, 664, 730, 850, 985, 1006
AjnI CCWGG 2 cut(s) 189, 939
AluBI AGCT 5 cut(s) 48, 92, 129, 595, 660
AluI AGCT 5 cut(s) 48, 92, 129, 595, 660
Alw21I GWGCWC 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 190
AoxI GGCC 1 cut(s) 915
ApeKI GCWGC 3 cut(s) 89, 533, 972
ApoI RAATTY 1 cut(s) 781
ArsI GACNNNNNNTTYG 2 cut(s) 513, 545
Asp700I GAANNNNTTC 2 cut(s) 101, 287
AspLEI GCGC 2 cut(s) 189, 223
AspS9I GGNCC 1 cut(s) 937
AsuC2I CCSGG 2 cut(s) 892, 914
AsuHPI GGTGA 2 cut(s) 427, 484
AsuII TTCGAA 2 cut(s) 95, 813
AvaII GGWCC 1 cut(s) 937
BaeGI GKGCMC 1 cut(s) 451
BaeI ACNNNNGTAYC 2 cut(s) 829, 862
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 131
BauI CACGAG 1 cut(s) 124
BbsI GAAGAC 5 cut(s) 635, 759, 802, 822, 879
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 3 cut(s) 101, 520, 984
BccI CCATC 2 cut(s) 719, 988
BceAI ACGGC 4 cut(s) 133, 482, 572, 772
BciT130I CCWGG 2 cut(s) 191, 941
BcnI CCSGG 2 cut(s) 892, 914
BfaI CTAG 2 cut(s) 437, 620
BfoI RGCGCY 1 cut(s) 190
BisI GCNGC 6 cut(s) 90, 219, 423, 534, 738, 973
BlsI GCNGC 6 cut(s) 91, 220, 424, 535, 739, 974
Bme1390I CCNGG 4 cut(s) 191, 892, 914, 941
Bme18I GGWCC 1 cut(s) 937
BmgT120I GGNCC 1 cut(s) 937
BmiI GGNNCC 2 cut(s) 84, 121
BmrFI CCNGG 4 cut(s) 191, 892, 914, 941
BmsI GCATC 4 cut(s) 142, 164, 316, 784
BoxI GACNNNNGTC 3 cut(s) 137, 179, 569
BpiI GAAGAC 5 cut(s) 635, 759, 802, 822, 879
BplI GAGNNNNNCTC 1 cut(s) 1000
BpmI CTGGAG 1 cut(s) 209
Bpu14I TTCGAA 2 cut(s) 95, 813
BpuMI CCSGG 2 cut(s) 892, 914
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 4 cut(s) 115, 166, 232, 912
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 504, 934
Bse1I ACTGG 1 cut(s) 566
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 2 cut(s) 191, 941
BseDI CCNNGG 4 cut(s) 115, 166, 232, 912
BseGI GGATG 3 cut(s) 157, 799, 954
BseLI CCNNNNNNNGG 3 cut(s) 190, 504, 934
BseMI GCAATG 1 cut(s) 654
BseNI ACTGG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 3 cut(s) 101, 520, 984
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 2 cut(s) 531, 835
BshFI GGCC 1 cut(s) 917
BshNI GGYRCC 1 cut(s) 119
BsiEI CGRYCG 2 cut(s) 531, 835
BsiHKAI GWGCWC 1 cut(s) 131
BsiSI CCGG 3 cut(s) 542, 892, 914
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 3 cut(s) 190, 504, 934
BsmFI GGGAC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 1 cut(s) 917
Bsp119I TTCGAA 2 cut(s) 95, 813
Bsp1286I GDGCHC 2 cut(s) 131, 451
Bsp143I GATC 1 cut(s) 376
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 6 cut(s) 87, 219, 423, 495, 737, 740
BspANI GGCC 1 cut(s) 917
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 84, 121
BspT104I TTCGAA 2 cut(s) 95, 813
BspT107I GGYRCC 1 cut(s) 119
BsrBI CCGCTC 1 cut(s) 737
BsrDI GCAATG 1 cut(s) 654
BsrI ACTGG 1 cut(s) 566
BssECI CCNNGG 4 cut(s) 115, 166, 232, 912
BssMI GATC 1 cut(s) 376
BssNI GRCGYC 1 cut(s) 570
BssSI CACGAG 1 cut(s) 124
BssT1I CCWWGG 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 124
Bst2UI CCWGG 2 cut(s) 191, 941
Bst4CI ACNGT 5 cut(s) 139, 211, 529, 772, 833
BstACI GRCGYC 1 cut(s) 570
BstBI TTCGAA 2 cut(s) 95, 813
BstC8I GCNNGC 3 cut(s) 9, 603, 761
BstDEI CTNAG 2 cut(s) 49, 787
BstDSI CCRYGG 2 cut(s) 115, 232
BstF5I GGATG 3 cut(s) 157, 799, 954
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 2 cut(s) 189, 223
BstKTI GATC 1 cut(s) 379
BstMBI GATC 1 cut(s) 376
BstMCI CGRYCG 2 cut(s) 531, 835
BstMWI GCNNNNNNNGC 3 cut(s) 89, 126, 345
BstNI CCWGG 2 cut(s) 191, 941
BstNSI RCATGY 2 cut(s) 447, 605
BstPAI GACNNNNGTC 3 cut(s) 137, 179, 569
BstSCI CCNGG 4 cut(s) 189, 890, 912, 939
BstSLI GKGCMC 1 cut(s) 451
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 3 cut(s) 101, 520, 984
BstV2I GAAGAC 5 cut(s) 635, 759, 802, 822, 879
BstXI CCANNNNNNTGG 1 cut(s) 233
BsuRI GGCC 1 cut(s) 917
BtgI CCRYGG 2 cut(s) 115, 232
BtsCI GGATG 3 cut(s) 157, 799, 954
BtsIMutI CAGTG 1 cut(s) 559
Cac8I GCNNGC 3 cut(s) 9, 603, 761
CaiI CAGNNNCTG 1 cut(s) 190
CfoI GCGC 2 cut(s) 189, 223
Cfr13I GGNCC 1 cut(s) 937
CseI GACGC 2 cut(s) 796, 884
Csp6I GTAC 1 cut(s) 724
CviAII CATG 3 cut(s) 233, 444, 602
CviQI GTAC 1 cut(s) 724
DdeI CTNAG 2 cut(s) 49, 787
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
EciI GGCGGA 1 cut(s) 755
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 937
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 189, 939
EcoT14I CCWWGG 1 cut(s) 232
EcoT22I ATGCAT 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 232
FaeI CATG 3 cut(s) 236, 447, 605
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 1 cut(s) 174
FatI CATG 3 cut(s) 232, 443, 601
FauI CCCGC 1 cut(s) 94
FauNDI CATATG 1 cut(s) 842
Fnu4HI GCNGC 6 cut(s) 90, 219, 423, 534, 738, 973
FokI GGATG 3 cut(s) 164, 806, 941
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 6 cut(s) 90, 219, 423, 534, 738, 973
FspBI CTAG 2 cut(s) 437, 620
GlaI GCGC 2 cut(s) 188, 222
GluI GCNGC 6 cut(s) 90, 219, 423, 534, 738, 973
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 1 cut(s) 917
HapII CCGG 3 cut(s) 542, 892, 914
HgaI GACGC 2 cut(s) 796, 884
HhaI GCGC 2 cut(s) 189, 223
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 3 cut(s) 236, 447, 605
Hin6I GCGC 2 cut(s) 187, 221
HinP1I GCGC 2 cut(s) 187, 221
HincII GTYRAC 3 cut(s) 574, 883, 900
HindII GTYRAC 3 cut(s) 574, 883, 900
HindIII AAGCTT 1 cut(s) 46
HinfI GANTC 3 cut(s) 97, 239, 951
HpaII CCGG 3 cut(s) 542, 892, 914
HphI GGTGA 2 cut(s) 427, 484
Hpy166II GTNNAC 5 cut(s) 214, 480, 574, 883, 900
Hpy188I TCNGA 2 cut(s) 198, 871
Hpy188III TCNNGA 5 cut(s) 159, 226, 437, 701, 1006
Hpy8I GTNNAC 5 cut(s) 214, 480, 574, 883, 900
Hpy99I CGWCG 4 cut(s) 110, 503, 529, 776
HpyAV CCTTC 3 cut(s) 328, 724, 915
HpyCH4III ACNGT 5 cut(s) 139, 211, 529, 772, 833
HpyCH4IV ACGT 2 cut(s) 432, 570
HpyCH4V TGCA 2 cut(s) 155, 683
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 126, 345
HpyF3I CTNAG 2 cut(s) 49, 787
HpySE526I ACGT 2 cut(s) 432, 570
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 3 cut(s) 236, 447, 605
HspAI GCGC 2 cut(s) 187, 221
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 5 cut(s) 88, 228, 507, 657, 734
Lsp1109I GCAGC 3 cut(s) 101, 520, 984
LweI GCATC 4 cut(s) 142, 164, 316, 784
MaeI CTAG 2 cut(s) 437, 620
MaeII ACGT 2 cut(s) 432, 570
MaeIII GTNAC 3 cut(s) 490, 512, 766
MalI GATC 1 cut(s) 378
MbiI CCGCTC 1 cut(s) 737
MboI GATC 1 cut(s) 376
MboII GAAGA 6 cut(s) 295, 640, 764, 802, 827, 884
MhlI GDGCHC 2 cut(s) 131, 451
MluCI AATT 1 cut(s) 781
MmeI TCCRAC 2 cut(s) 528, 849
MnlI CCTC 8 cut(s) 66, 176, 188, 192, 270, 378, 406, 928
Mph1103I ATGCAT 1 cut(s) 157
MroXI GAANNNNTTC 2 cut(s) 101, 287
MspI CCGG 3 cut(s) 542, 892, 914
MspR9I CCNGG 4 cut(s) 191, 892, 914, 941
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 2 cut(s) 191, 941
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 3 cut(s) 89, 126, 345
NciI CCSGG 2 cut(s) 892, 914
NcoI CCATGG 1 cut(s) 232
NdeI CATATG 1 cut(s) 842
NdeII GATC 1 cut(s) 376
NlaIII CATG 3 cut(s) 236, 447, 605
NlaIV GGNNCC 2 cut(s) 84, 121
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 2 cut(s) 490, 766
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 2 cut(s) 447, 605
NspV TTCGAA 2 cut(s) 95, 813
PaeI GCATGC 1 cut(s) 605
PctI GAATGC 1 cut(s) 685
PdmI GAANNNNTTC 2 cut(s) 101, 287
PfeI GAWTC 3 cut(s) 97, 239, 951
PflMI CCANNNNNTGG 1 cut(s) 190
PkrI GCNGC 6 cut(s) 91, 220, 424, 535, 739, 974
PshAI GACNNNNGTC 3 cut(s) 137, 179, 569
Psp124BI GAGCTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 189, 939
PspGI CCWGG 2 cut(s) 189, 939
PspN4I GGNNCC 2 cut(s) 84, 121
PspPI GGNCC 1 cut(s) 937
PstNI CAGNNNCTG 1 cut(s) 190
RsaI GTAC 1 cut(s) 725
RsaNI GTAC 1 cut(s) 724
SacI GAGCTC 1 cut(s) 131
SatI GCNGC 6 cut(s) 90, 219, 423, 534, 738, 973
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 937
ScrFI CCNGG 4 cut(s) 191, 892, 914, 941
SduI GDGCHC 2 cut(s) 131, 451
SfaNI GCATC 4 cut(s) 142, 164, 316, 784
SfuI TTCGAA 2 cut(s) 95, 813
SinI GGWCC 1 cut(s) 937
SphI GCATGC 1 cut(s) 605
Sse9I AATT 1 cut(s) 781
SsiI CCGC 6 cut(s) 87, 219, 423, 495, 737, 740
SspMI CTAG 2 cut(s) 437, 620
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 4 cut(s) 189, 890, 912, 939
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 5 cut(s) 139, 211, 529, 772, 833
TaiI ACGT 2 cut(s) 435, 573
TaqI TCGA 8 cut(s) 95, 105, 131, 173, 524, 774, 813, 835
TasI AATT 1 cut(s) 781
TauI GCSGC 3 cut(s) 221, 425, 740
TfiI GAWTC 3 cut(s) 97, 239, 951
TscAI CASTG 1 cut(s) 566
TseFI GTSAC 2 cut(s) 490, 766
TseI GCWGC 3 cut(s) 89, 533, 972
Tsp45I GTSAC 2 cut(s) 490, 766
TspDTI ATGAA 3 cut(s) 408, 692, 765
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 1 cut(s) 566
Van91I CCANNNNNTGG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 937
XapI RAATTY 1 cut(s) 781
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 2 cut(s) 447, 605
XmnI GAANNNNTTC 2 cut(s) 101, 287
XspI CTAG 2 cut(s) 437, 620
ZraI GACGTC 1 cut(s) 571
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.