Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001892

Physical Location & Seq

Genomic Coordinates Reverse (-)

4675577 .. 4679818

Gene Structure 4242 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1036600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 900 bp

ATGGCACCAGCAGCAGCAGTATGTGTAATGGATGCATCAGGCCACGTAGGCGCCAGCCTCACAGAGCGCCTGCTGCAAAAGGGTTACACAGTACATGCTGCCCTTCAGAGGCATGGGGAAGCGCGGTTGGGTGGGATTTGTTGTGACAAAACGAAGCTCAAAGTTTTCGATCTGGATCCGTTCGACTACCAGAGCATCCTCGACGCTCTCAAAGGCTGTTCCGGCTTGTTTTATTCATTTGAGCCTCCACAAGATCAGCCTGACTGCGATGAATACATGGCAGAAGTGGAGGTTCGGGCAGCGCACAACGTACTAGAAGCATGTGCAAGAACTGAAACAATAGACAAAATCGTGTTTACATCTTCCGCCGCTGCCGTCTGTTGGCGAAACGATCGCAAGTCGACGGCTCTCGATTTGGATTTGGATGAACGGCACTGGAGCGATGTCAATTTCTGTCGTAGTTTCAAGTTGTGGCATGCATTATCAAAAACCCTAGCAGAGAAGACAGCATGGGCCCTTGCAATGGACAGGAGCTTGAATATGGTGTCTATGAATGTAGGTTTGCTGATGGCTCCGGATTTGTCCATCACCAACCCGTATTTGAAAGGAGCAGCTGAGATGTACGAAGATGGCGTGCTCGTGACCGTCGATACTGATTTCTTAGTCGATGCACACATCTGCGTTTTTGAAGATGTCTCGTCCTACGGTCGATATCTGTGCTTCAACAACATCATCAATCGGGCTGAAGATGCTCTAGAGCTTGCTCGGAAGTTGACTCCTTCTGCCCCCTCATACCCACAAAGCCAAGATCAGGATATGAGGATCCCTCAACAGAGGATAAGCAACAAGAAATTAAATAAATTGATGGTGGAATTTAAGAGCAAATCTCAAGAGTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

300

Amino Acids

33.39

Weight (kDa)

5.22

Isoelectric Point (pI)

40.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
3Beta_HSD	PF01073	9 - 168	2.3e-06	3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase	PF01370	10 - 191	3.8e-07	NAD dependent epimerase/dehydratase family
NAD_binding_10	PF13460	12 - 125	1.4e-07	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23910 AT2G23910 AT2G23910 AT2G23910 AT4G30470
fragaria_vesca	FvH4_5g22420 FvH4_5g22420 FvH4_5g22420
malus_domestica	MD00G1072500.v1.1 MD01G1237600.v1.1 MD06G1036600.v1.1 MD07G1310200.v1.1
prunus_persica	Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.5G032800_v2.0.a1
pyrus_communis	pycom01g24530 pycom06g03000 pycom13g28670
rosa_chinensis	RchiOBHm_Chr4g0405161 RchiOBHm_Chr7g0210161
rosa_laevigata	RLG00000003059 RLG00000008867
rosa_multiflora	Rmu_sc0005438.1_g000001 Rmu_sc0006908.1_g000015 Rmu_ssc0000255.1_g000034
rosa_roxburghii	Rroxscaffold_3G00248570 Rroxscaffold_5G00349400
rosa_rugosa	Rorug04G0054400 Rorug04G0054500 Rorug04G0054600 Rorug07G0119000
rosa_samantha	Rh1AG349400 Rh4AG129400 Rh4BG123100 Rh4CG136700 Rh4DG122900 Rh7AG252500 Rh7BG247600 Rh7CG269500 Rh7DG260100
rosa_wichuraiana	Rw0G002460 Rw1G031120 Rw4G010450 Rw7G021460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	4, 50
AccI	GTMKAC	1 cut(s)	401
AccII	CGCG	1 cut(s)	124
AccIII	TCCGGA	1 cut(s)	574
AciI	CCGC	3 cut(s)	124, 366, 369
AclWI	GGATC	4 cut(s)	170, 183, 817, 830
AcsI	RAATTY	1 cut(s)	872
AcuI	CTGAAG	2 cut(s)	89, 765
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	3 cut(s)	93, 312, 623
AfiI	CCNNNNNNNGG	4 cut(s)	108, 381, 523, 811
AgsI	TTSAA	5 cut(s)	466, 538, 604, 689, 724
AluBI	AGCT	4 cut(s)	157, 534, 614, 760
AluI	AGCT	4 cut(s)	157, 534, 614, 760
Alw21I	GWGCWC	1 cut(s)	639
Alw26I	GTCTC	1 cut(s)	700
AlwI	GGATC	4 cut(s)	170, 183, 817, 830
Aor13HI	TCCGGA	1 cut(s)	574
AoxI	GGCC	2 cut(s)	40, 513
ApaI	GGGCCC	1 cut(s)	517
ApeKI	GCWGC	7 cut(s)	11, 14, 73, 98, 299, 371, 611
ApoI	RAATTY	1 cut(s)	872
AspLEI	GCGC	4 cut(s)	53, 69, 124, 304
AspS9I	GGNCC	2 cut(s)	513, 514
AsuHPI	GGTGA	1 cut(s)	580
BaeGI	GKGCMC	1 cut(s)	517
BamHI	GGATCC	2 cut(s)	175, 822
BanI	GGYRCC	2 cut(s)	4, 50
BanII	GRGCYC	1 cut(s)	517
BauI	CACGAG	1 cut(s)	638
BbsI	GAAGAC	1 cut(s)	509
Bbv12I	GWGCWC	1 cut(s)	639
BbvI	GCAGC	7 cut(s)	23, 26, 60, 85, 311, 358, 623
BccI	CCATC	4 cut(s)	562, 593, 623, 859
BceAI	ACGGC	3 cut(s)	359, 420, 446
BcgI	CGANNNNNNTGC	2 cut(s)	699, 733
BcoDI	GTCTC	1 cut(s)	700
BfaI	CTAG	3 cut(s)	314, 494, 755
BfoI	RGCGCY	2 cut(s)	54, 70
BglI	GCCNNNNNGGC	1 cut(s)	48
BisI	GCNGC	8 cut(s)	12, 15, 74, 99, 300, 369, 372, 612
BlsI	GCNGC	8 cut(s)	13, 16, 75, 100, 301, 370, 373, 613
BmgT120I	GGNCC	2 cut(s)	513, 514
BmiI	GGNNCC	6 cut(s)	6, 52, 177, 515, 573, 824
BmsI	GCATC	5 cut(s)	22, 44, 204, 658, 739
BpiI	GAAGAC	1 cut(s)	509
BplI	GAGNNNNNCTC	3 cut(s)	811, 843, 871
BpmI	CTGGAG	1 cut(s)	457
BpuEI	CTTGAG	1 cut(s)	873
BsaAI	YACGTR	1 cut(s)	46
BsaBI	GATNNNNATC	1 cut(s)	174
BsaHI	GRCGYC	1 cut(s)	51
BsaWI	WCCGGW	1 cut(s)	574
Bsc4I	CCNNNNNNNGG	4 cut(s)	108, 381, 523, 811
Bse1I	ACTGG	1 cut(s)	440
Bse3DI	GCAATG	1 cut(s)	528
Bse8I	GATNNNNATC	1 cut(s)	174
BseAI	TCCGGA	1 cut(s)	574
BseGI	GGATG	3 cut(s)	37, 195, 430
BseJI	GATNNNNATC	1 cut(s)	174
BseLI	CCNNNNNNNGG	4 cut(s)	108, 381, 523, 811
BseMI	GCAATG	1 cut(s)	528
BseMII	CTCAG	1 cut(s)	606
BseNI	ACTGG	1 cut(s)	440
BseSI	GKGCMC	1 cut(s)	517
BseXI	GCAGC	7 cut(s)	23, 26, 60, 85, 311, 358, 623
Bsh1236I	CGCG	1 cut(s)	124
Bsh1285I	CGRYCG	2 cut(s)	394, 709
BshFI	GGCC	2 cut(s)	42, 515
BshNI	GGYRCC	2 cut(s)	4, 50
BsiEI	CGRYCG	2 cut(s)	394, 709
BsiHKAI	GWGCWC	1 cut(s)	639
BsiSI	CCGG	2 cut(s)	222, 575
BslI	CCNNNNNNNGG	4 cut(s)	108, 381, 523, 811
BsmAI	GTCTC	1 cut(s)	700
BsnI	GGCC	2 cut(s)	42, 515
Bsp120I	GGGCCC	1 cut(s)	513
Bsp1286I	GDGCHC	2 cut(s)	517, 639
Bsp13I	TCCGGA	1 cut(s)	574
Bsp143I	GATC	6 cut(s)	169, 175, 253, 391, 808, 822
BspACI	CCGC	3 cut(s)	124, 366, 369
BspANI	GGCC	2 cut(s)	42, 515
BspCNI	CTCAG	1 cut(s)	607
BspEI	TCCGGA	1 cut(s)	574
BspFNI	CGCG	1 cut(s)	124
BspLI	GGNNCC	6 cut(s)	6, 52, 177, 515, 573, 824
BspPI	GGATC	4 cut(s)	170, 183, 817, 830
BspT107I	GGYRCC	2 cut(s)	4, 50
BsrDI	GCAATG	1 cut(s)	528
BsrI	ACTGG	1 cut(s)	440
BssMI	GATC	6 cut(s)	169, 175, 253, 391, 808, 822
BssNI	GRCGYC	1 cut(s)	51
BssSI	CACGAG	1 cut(s)	638
Bst2BI	CACGAG	1 cut(s)	638
Bst4CI	ACNGT	3 cut(s)	91, 646, 707
BstACI	GRCGYC	1 cut(s)	51
BstBAI	YACGTR	1 cut(s)	46
BstC8I	GCNNGC	5 cut(s)	55, 71, 477, 635, 762
BstDEI	CTNAG	2 cut(s)	615, 661
BstF5I	GGATG	3 cut(s)	37, 195, 430
BstFNI	CGCG	1 cut(s)	124
BstH2I	RGCGCY	2 cut(s)	54, 70
BstHHI	GCGC	4 cut(s)	53, 69, 124, 304
BstKTI	GATC	6 cut(s)	172, 178, 256, 394, 811, 825
BstMAI	GTCTC	1 cut(s)	700
BstMBI	GATC	6 cut(s)	169, 175, 253, 391, 808, 822
BstMCI	CGRYCG	2 cut(s)	394, 709
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 48, 73, 222, 749
BstNSI	RCATGY	3 cut(s)	98, 324, 479
BstSLI	GKGCMC	1 cut(s)	517
BstUI	CGCG	1 cut(s)	124
BstV1I	GCAGC	7 cut(s)	23, 26, 60, 85, 311, 358, 623
BstV2I	GAAGAC	1 cut(s)	509
BstX2I	RGATCY	2 cut(s)	175, 822
BstYI	RGATCY	2 cut(s)	175, 822
BsuRI	GGCC	2 cut(s)	42, 515
BtgZI	GCGATG	2 cut(s)	282, 456
BtsCI	GGATG	3 cut(s)	37, 195, 430
BtsIMutI	CAGTG	1 cut(s)	433
Cac8I	GCNNGC	5 cut(s)	55, 71, 477, 635, 762
CfoI	GCGC	4 cut(s)	53, 69, 124, 304
Cfr13I	GGNCC	2 cut(s)	513, 514
CseI	GACGC	1 cut(s)	212
Csp6I	GTAC	3 cut(s)	92, 311, 622
CviAII	CATG	6 cut(s)	95, 113, 277, 321, 476, 510
CviQI	GTAC	3 cut(s)	92, 311, 622
DdeI	CTNAG	2 cut(s)	615, 661
DinI	GGCGCC	1 cut(s)	52
DpnI	GATC	6 cut(s)	171, 177, 255, 393, 810, 824
DpnII	GATC	6 cut(s)	169, 175, 253, 391, 808, 822
EciI	GGCGGA	1 cut(s)	355
Eco24I	GRGCYC	1 cut(s)	517
Eco32I	GATATC	1 cut(s)	713
Eco57I	CTGAAG	2 cut(s)	89, 765
EcoO109I	RGGNCCY	1 cut(s)	514
EcoRV	GATATC	1 cut(s)	713
EcoT22I	ATGCAT	2 cut(s)	37, 481
EcoT38I	GRGCYC	1 cut(s)	517
EgeI	GGCGCC	1 cut(s)	52
EheI	GGCGCC	1 cut(s)	52
FaeI	CATG	6 cut(s)	98, 116, 280, 324, 479, 513
FatI	CATG	6 cut(s)	94, 112, 276, 320, 475, 509
FblI	GTMKAC	1 cut(s)	401
Fnu4HI	GCNGC	8 cut(s)	12, 15, 74, 99, 300, 369, 372, 612
FokI	GGATG	3 cut(s)	44, 182, 437
FriOI	GRGCYC	1 cut(s)	517
Fsp4HI	GCNGC	8 cut(s)	12, 15, 74, 99, 300, 369, 372, 612
FspBI	CTAG	3 cut(s)	314, 494, 755
GlaI	GCGC	4 cut(s)	52, 68, 123, 303
GluI	GCNGC	8 cut(s)	12, 15, 74, 99, 300, 369, 372, 612
GsuI	CTGGAG	1 cut(s)	457
HaeII	RGCGCY	2 cut(s)	54, 70
HaeIII	GGCC	2 cut(s)	42, 515
HapII	CCGG	2 cut(s)	222, 575
HgaI	GACGC	1 cut(s)	212
HhaI	GCGC	4 cut(s)	53, 69, 124, 304
Hin1I	GRCGYC	1 cut(s)	51
Hin1II	CATG	6 cut(s)	98, 116, 280, 324, 479, 513
Hin6I	GCGC	4 cut(s)	51, 67, 122, 302
HinP1I	GCGC	4 cut(s)	51, 67, 122, 302
HincII	GTYRAC	2 cut(s)	402, 774
HindII	GTYRAC	2 cut(s)	402, 774
HinfI	GANTC	1 cut(s)	775
HpaII	CCGG	2 cut(s)	222, 575
HphI	GGTGA	1 cut(s)	580
Hpy166II	GTNNAC	3 cut(s)	357, 402, 774
Hpy188I	TCNGA	2 cut(s)	108, 768
Hpy188III	TCNNGA	7 cut(s)	173, 410, 575, 640, 755, 812, 890
Hpy8I	GTNNAC	3 cut(s)	357, 402, 774
Hpy99I	CGWCG	3 cut(s)	206, 406, 650
HpyAV	CCTTC	2 cut(s)	113, 789
HpyCH4III	ACNGT	3 cut(s)	91, 646, 707
HpyCH4IV	ACGT	2 cut(s)	45, 309
HpyCH4V	TGCA	6 cut(s)	35, 76, 326, 479, 521, 671
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 48, 73, 222, 749
HpyF3I	CTNAG	2 cut(s)	615, 661
HpySE526I	ACGT	2 cut(s)	45, 309
Hsp92I	GRCGYC	1 cut(s)	51
Hsp92II	CATG	6 cut(s)	98, 116, 280, 324, 479, 513
HspAI	GCGC	4 cut(s)	51, 67, 122, 302
KasI	GGCGCC	1 cut(s)	50
Kpn2I	TCCGGA	1 cut(s)	574
Kzo9I	GATC	6 cut(s)	169, 175, 253, 391, 808, 822
LmnI	GCTCC	4 cut(s)	438, 531, 577, 608
Lsp1109I	GCAGC	7 cut(s)	23, 26, 60, 85, 311, 358, 623
LweI	GCATC	5 cut(s)	22, 44, 204, 658, 739
MaeI	CTAG	3 cut(s)	314, 494, 755
MaeII	ACGT	2 cut(s)	45, 309
MaeIII	GTNAC	3 cut(s)	83, 143, 640
MalI	GATC	6 cut(s)	171, 177, 255, 393, 810, 824
MboI	GATC	6 cut(s)	169, 175, 253, 391, 808, 822
MboII	GAAGA	5 cut(s)	354, 514, 638, 701, 758
MflI	RGATCY	2 cut(s)	175, 822
MhlI	GDGCHC	2 cut(s)	517, 639
MluCI	AATT	4 cut(s)	448, 851, 860, 872
Mly113I	GGCGCC	1 cut(s)	51
MlyI	GAGTC	1 cut(s)	769
MnlI	CCTC	9 cut(s)	68, 102, 209, 255, 283, 799, 813, 828, 837
Mph1103I	ATGCAT	2 cut(s)	37, 481
MroI	TCCGGA	1 cut(s)	574
MseI	TTAA	2 cut(s)	854, 876
MspA1I	CMGCKG	2 cut(s)	371, 614
MspI	CCGG	2 cut(s)	222, 575
MvnI	CGCG	1 cut(s)	124
MwoI	GCNNNNNNNGC	5 cut(s)	11, 48, 73, 222, 749
NarI	GGCGCC	1 cut(s)	51
NdeII	GATC	6 cut(s)	169, 175, 253, 391, 808, 822
NlaIII	CATG	6 cut(s)	98, 116, 280, 324, 479, 513
NlaIV	GGNNCC	6 cut(s)	6, 52, 177, 515, 573, 824
NmuCI	GTSAC	2 cut(s)	143, 640
NsiI	ATGCAT	2 cut(s)	37, 481
NspI	RCATGY	3 cut(s)	98, 324, 479
PaeI	GCATGC	1 cut(s)	479
PcsI	WCGNNNNNNNCGW	2 cut(s)	630, 645
PkrI	GCNGC	8 cut(s)	13, 16, 75, 100, 301, 370, 373, 613
Ple19I	CGATCG	1 cut(s)	394
PleI	GAGTC	1 cut(s)	769
PluTI	GGCGCC	1 cut(s)	54
PpsI	GAGTC	1 cut(s)	769
Ppu21I	YACGTR	1 cut(s)	46
PspN4I	GGNNCC	6 cut(s)	6, 52, 177, 515, 573, 824
PspOMI	GGGCCC	1 cut(s)	513
PspPI	GGNCC	2 cut(s)	513, 514
PsuI	RGATCY	2 cut(s)	175, 822
PvuI	CGATCG	1 cut(s)	394
PvuII	CAGCTG	1 cut(s)	614
RsaI	GTAC	3 cut(s)	93, 312, 623
RsaNI	GTAC	3 cut(s)	92, 311, 622
SalI	GTCGAC	1 cut(s)	400
SaqAI	TTAA	2 cut(s)	854, 876
SatI	GCNGC	8 cut(s)	12, 15, 74, 99, 300, 369, 372, 612
Sau3AI	GATC	6 cut(s)	169, 175, 253, 391, 808, 822
Sau96I	GGNCC	2 cut(s)	513, 514
SchI	GAGTC	1 cut(s)	769
SduI	GDGCHC	2 cut(s)	517, 639
SetI	ASST	8 cut(s)	48, 159, 294, 312, 536, 562, 616, 762
SfaNI	GCATC	5 cut(s)	22, 44, 204, 658, 739
SfoI	GGCGCC	1 cut(s)	52
SmlI	CTYRAG	1 cut(s)	888
SmoI	CTYRAG	1 cut(s)	888
SphI	GCATGC	1 cut(s)	479
Sse9I	AATT	4 cut(s)	448, 851, 860, 872
SsiI	CCGC	3 cut(s)	124, 366, 369
SspDI	GGCGCC	1 cut(s)	50
SspMI	CTAG	3 cut(s)	314, 494, 755
TaaI	ACNGT	3 cut(s)	91, 646, 707
TaiI	ACGT	2 cut(s)	48, 312
TaqI	TCGA	8 cut(s)	168, 183, 201, 401, 411, 648, 666, 709
TasI	AATT	4 cut(s)	448, 851, 860, 872
TatI	WGTACW	1 cut(s)	91
TauI	GCSGC	1 cut(s)	371
Tru1I	TTAA	2 cut(s)	854, 876
Tru9I	TTAA	2 cut(s)	854, 876
TscAI	CASTG	1 cut(s)	440
TseFI	GTSAC	2 cut(s)	143, 640
TseI	GCWGC	7 cut(s)	11, 14, 73, 98, 299, 371, 611
Tsp45I	GTSAC	2 cut(s)	143, 640
TspDTI	ATGAA	4 cut(s)	225, 285, 441, 566
TspGWI	ACGGA	1 cut(s)	168
TspRI	CASTG	1 cut(s)	440
XapI	RAATTY	1 cut(s)	872
XbaI	TCTAGA	1 cut(s)	754
XceI	RCATGY	3 cut(s)	98, 324, 479
XmiI	GTMKAC	1 cut(s)	401
XspI	CTAG	3 cut(s)	314, 494, 755
Zsp2I	ATGCAT	2 cut(s)	37, 481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.