Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001892

Physical Location & Seq

Genomic Coordinates Forward (+)

14478721 .. 14482627

Gene Structure 3907 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1072500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 921 bp

ATGGCACCAACAGCAGCATCGGACTCTTCGAACAGAGCTGTATGTGTAATGGATGCATCAGGCCACTTAGGCACCAGCCTCGCTGAGCACCTGCTGCAAAAGGGTTACACAGTCCATGCTGCCCTTCAGAAACATGGGGAATCGCGGTTGAGTGGGATTTCCTGTGACAAAAAGAAGCTCAAAGTTTTCAATCTGGATCCGTTCGACTACCAGAGCATTCTCGACGCTCTCAAAGGCTGTTCTGGCTTGTTTTATTCATTTGAGCCTCCACAAGAACAACCAGACTATGATGAATACATGACAGAAGTAGAGGTTCGAGCAGCACACAACGTACTAGAAGCCTGTGCAAGAACTGAAACAATAGACAAAGTCGTGTTTACATCTTCCGCCGCTGCTGTCCTTTGGCGAAACGATCGCAAATCGACAACTCTTGATTTGGATTTGGATGAACGACACTGGACCGATGTCAATTTCTGTCGTAGTTTCAAGTTGTGGCATGCATTATCAAAAACCCTAGCAGAGAAGACAGCATGGGCCTTGGCAATGGACAGGAGCTTGAATATGGTGTCTTTGAATGTAGGGTTGCTGATGGCTCCGGATTTGTCCATAACCAACCCATATTTGAAAGGAGCAGCTGAGATGTACGAAGATGGTGTGCTCGTGACGGTTGATACTGATTTCTTAGTCGATGCGCACATTTGCGTTTTTGAAGATGTCTCATCCTACGGTCGATATCTATGCTTCAACAACATCATCAATCGGTCTGAATATGCTATGGAGCTTGCTCGGAAGTTGACCCCTGCTACCCTTTCATACCCCGAACGCCAAGACCAGGACATGAGGATCCCTCGACAGAGAATAAGCAACAAGAAATTAAATAAATTGATGGTGGAATTTAAGAGCAAATCTCAAGAGTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

307

Amino Acids

34.5

Weight (kDa)

5.66

Isoelectric Point (pI)

40.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
3Beta_HSD	PF01073	16 - 175	2.3e-07	3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase	PF01370	17 - 199	2.3e-07	NAD dependent epimerase/dehydratase family
NAD_binding_10	PF13460	19 - 132	3.1e-07	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23910 AT2G23910 AT2G23910 AT2G23910 AT4G30470
fragaria_vesca	FvH4_5g22420 FvH4_5g22420 FvH4_5g22420
malus_domestica	MD00G1072500.v1.1 MD01G1237600.v1.1 MD06G1036600.v1.1 MD07G1310200.v1.1
prunus_persica	Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.1G099500_v2.0.a1 Prupe.5G032800_v2.0.a1
pyrus_communis	pycom01g24530 pycom06g03000 pycom13g28670
rosa_chinensis	RchiOBHm_Chr4g0405161 RchiOBHm_Chr7g0210161
rosa_laevigata	RLG00000003059 RLG00000008867
rosa_multiflora	Rmu_sc0005438.1_g000001 Rmu_sc0006908.1_g000015 Rmu_ssc0000255.1_g000034
rosa_roxburghii	Rroxscaffold_3G00248570 Rroxscaffold_5G00349400
rosa_rugosa	Rorug04G0054400 Rorug04G0054500 Rorug04G0054600 Rorug07G0119000
rosa_samantha	Rh1AG349400 Rh4AG129400 Rh4BG123100 Rh4CG136700 Rh4DG122900 Rh7AG252500 Rh7BG247600 Rh7CG269500 Rh7DG260100
rosa_wichuraiana	Rw0G002460 Rw1G031120 Rw4G010450 Rw7G021460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	99
Acc16I	TGCGCA	1 cut(s)	693
Acc36I	ACCTGC	1 cut(s)	99
AccB1I	GGYRCC	2 cut(s)	4, 71
AccII	CGCG	1 cut(s)	145
AccIII	TCCGGA	1 cut(s)	595
AciI	CCGC	3 cut(s)	145, 387, 390
AclWI	GGATC	4 cut(s)	191, 204, 838, 851
AcsI	RAATTY	1 cut(s)	893
AcuI	CTGAAG	1 cut(s)	110
AfaI	GTAC	2 cut(s)	333, 644
AfiI	CCNNNNNNNGG	1 cut(s)	832
AgsI	TTSAA	7 cut(s)	190, 487, 559, 574, 625, 710, 745
AjnI	CCWGG	1 cut(s)	831
AluBI	AGCT	5 cut(s)	38, 178, 555, 635, 781
AluI	AGCT	5 cut(s)	38, 178, 555, 635, 781
Alw21I	GWGCWC	2 cut(s)	90, 660
Alw26I	GTCTC	1 cut(s)	721
AlwI	GGATC	4 cut(s)	191, 204, 838, 851
Aor13HI	TCCGGA	1 cut(s)	595
AoxI	GGCC	2 cut(s)	61, 534
ApeKI	GCWGC	6 cut(s)	14, 94, 119, 320, 392, 632
ApoI	RAATTY	1 cut(s)	893
AspLEI	GCGC	1 cut(s)	694
AspS9I	GGNCC	2 cut(s)	459, 534
AsuII	TTCGAA	1 cut(s)	29
AvaII	GGWCC	1 cut(s)	459
BamHI	GGATCC	2 cut(s)	196, 843
BanI	GGYRCC	2 cut(s)	4, 71
BauI	CACGAG	1 cut(s)	659
BbsI	GAAGAC	1 cut(s)	530
Bbv12I	GWGCWC	2 cut(s)	90, 660
BbvI	GCAGC	6 cut(s)	26, 81, 106, 332, 379, 644
BccI	CCATC	3 cut(s)	583, 644, 880
BcgI	CGANNNNNNTGC	4 cut(s)	61, 95, 720, 754
BciT130I	CCWGG	1 cut(s)	833
BcoDI	GTCTC	1 cut(s)	721
BfaI	CTAG	2 cut(s)	335, 515
BfuAI	ACCTGC	1 cut(s)	99
BglI	GCCNNNNNGGC	1 cut(s)	69
BisI	GCNGC	7 cut(s)	15, 95, 120, 321, 390, 393, 633
BlpI	GCTNAGC	1 cut(s)	84
BlsI	GCNGC	7 cut(s)	16, 96, 121, 322, 391, 394, 634
Bme1390I	CCNGG	1 cut(s)	833
Bme18I	GGWCC	1 cut(s)	459
BmgT120I	GGNCC	2 cut(s)	459, 534
BmiI	GGNNCC	5 cut(s)	6, 73, 198, 594, 845
BmrFI	CCNGG	1 cut(s)	833
BmsI	GCATC	4 cut(s)	26, 43, 65, 679
BoxI	GACNNNNGTC	1 cut(s)	464
BpiI	GAAGAC	1 cut(s)	530
BplI	GAGNNNNNCTC	3 cut(s)	832, 864, 892
Bpu1102I	GCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	29
BpuEI	CTTGAG	1 cut(s)	894
BsaJI	CCNNGG	1 cut(s)	537
BsaWI	WCCGGW	1 cut(s)	595
Bsc4I	CCNNNNNNNGG	1 cut(s)	832
Bse1I	ACTGG	1 cut(s)	461
Bse3DI	GCAATG	1 cut(s)	549
BseAI	TCCGGA	1 cut(s)	595
BseBI	CCWGG	1 cut(s)	833
BseDI	CCNNGG	1 cut(s)	537
BseGI	GGATG	3 cut(s)	58, 451, 719
BseLI	CCNNNNNNNGG	1 cut(s)	832
BseMI	GCAATG	1 cut(s)	549
BseMII	CTCAG	2 cut(s)	75, 627
BseNI	ACTGG	1 cut(s)	461
BseXI	GCAGC	6 cut(s)	26, 81, 106, 332, 379, 644
Bsh1236I	CGCG	1 cut(s)	145
Bsh1285I	CGRYCG	2 cut(s)	415, 730
BshFI	GGCC	2 cut(s)	63, 536
BshNI	GGYRCC	2 cut(s)	4, 71
BsiEI	CGRYCG	2 cut(s)	415, 730
BsiHKAI	GWGCWC	2 cut(s)	90, 660
BsiSI	CCGG	1 cut(s)	596
BslI	CCNNNNNNNGG	1 cut(s)	832
BsmAI	GTCTC	1 cut(s)	721
BsmI	GAATGC	1 cut(s)	216
BsnI	GGCC	2 cut(s)	63, 536
Bsp119I	TTCGAA	1 cut(s)	29
Bsp1286I	GDGCHC	2 cut(s)	90, 660
Bsp13I	TCCGGA	1 cut(s)	595
Bsp143I	GATC	3 cut(s)	196, 412, 843
Bsp1720I	GCTNAGC	1 cut(s)	84
BspACI	CCGC	3 cut(s)	145, 387, 390
BspANI	GGCC	2 cut(s)	63, 536
BspCNI	CTCAG	2 cut(s)	76, 628
BspEI	TCCGGA	1 cut(s)	595
BspFNI	CGCG	1 cut(s)	145
BspLI	GGNNCC	5 cut(s)	6, 73, 198, 594, 845
BspMI	ACCTGC	1 cut(s)	99
BspPI	GGATC	4 cut(s)	191, 204, 838, 851
BspT104I	TTCGAA	1 cut(s)	29
BspT107I	GGYRCC	2 cut(s)	4, 71
BsrDI	GCAATG	1 cut(s)	549
BsrI	ACTGG	1 cut(s)	461
BssECI	CCNNGG	1 cut(s)	537
BssMI	GATC	3 cut(s)	196, 412, 843
BssSI	CACGAG	1 cut(s)	659
BssT1I	CCWWGG	1 cut(s)	537
Bst2BI	CACGAG	1 cut(s)	659
Bst2UI	CCWGG	1 cut(s)	833
Bst4CI	ACNGT	3 cut(s)	112, 667, 728
Bst6I	CTCTTC	1 cut(s)	31
BstAPI	GCANNNNNTGC	1 cut(s)	94
BstBI	TTCGAA	1 cut(s)	29
BstC8I	GCNNGC	2 cut(s)	498, 783
BstDEI	CTNAG	4 cut(s)	67, 84, 636, 682
BstF5I	GGATG	3 cut(s)	58, 451, 719
BstFNI	CGCG	1 cut(s)	145
BstHHI	GCGC	1 cut(s)	694
BstKTI	GATC	3 cut(s)	199, 415, 846
BstMAI	GTCTC	1 cut(s)	721
BstMBI	GATC	3 cut(s)	196, 412, 843
BstMCI	CGRYCG	2 cut(s)	415, 730
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 69, 94, 243
BstNI	CCWGG	1 cut(s)	833
BstNSI	RCATGY	1 cut(s)	500
BstPAI	GACNNNNGTC	1 cut(s)	464
BstSCI	CCNGG	1 cut(s)	831
BstUI	CGCG	1 cut(s)	145
BstV1I	GCAGC	6 cut(s)	26, 81, 106, 332, 379, 644
BstV2I	GAAGAC	1 cut(s)	530
BstX2I	RGATCY	2 cut(s)	196, 843
BstYI	RGATCY	2 cut(s)	196, 843
BsuRI	GGCC	2 cut(s)	63, 536
BtsCI	GGATG	3 cut(s)	58, 451, 719
BtsIMutI	CAGTG	1 cut(s)	454
BveI	ACCTGC	1 cut(s)	99
Cac8I	GCNNGC	2 cut(s)	498, 783
CfoI	GCGC	1 cut(s)	694
Cfr13I	GGNCC	2 cut(s)	459, 534
CseI	GACGC	1 cut(s)	233
Csp6I	GTAC	2 cut(s)	332, 643
CviAII	CATG	6 cut(s)	116, 134, 298, 497, 531, 838
CviQI	GTAC	2 cut(s)	332, 643
DdeI	CTNAG	4 cut(s)	67, 84, 636, 682
DpnI	GATC	3 cut(s)	198, 414, 845
DpnII	GATC	3 cut(s)	196, 412, 843
Eam1104I	CTCTTC	1 cut(s)	31
EarI	CTCTTC	1 cut(s)	31
EciI	GGCGGA	1 cut(s)	376
Eco130I	CCWWGG	1 cut(s)	537
Eco32I	GATATC	1 cut(s)	734
Eco47I	GGWCC	1 cut(s)	459
Eco57I	CTGAAG	1 cut(s)	110
EcoRII	CCWGG	1 cut(s)	831
EcoRV	GATATC	1 cut(s)	734
EcoT14I	CCWWGG	1 cut(s)	537
EcoT22I	ATGCAT	2 cut(s)	58, 502
ErhI	CCWWGG	1 cut(s)	537
FaeI	CATG	6 cut(s)	119, 137, 301, 500, 534, 841
FatI	CATG	6 cut(s)	115, 133, 297, 496, 530, 837
Fnu4HI	GCNGC	7 cut(s)	15, 95, 120, 321, 390, 393, 633
FokI	GGATG	3 cut(s)	65, 458, 706
Fsp4HI	GCNGC	7 cut(s)	15, 95, 120, 321, 390, 393, 633
FspAI	RTGCGCAY	1 cut(s)	693
FspBI	CTAG	2 cut(s)	335, 515
FspI	TGCGCA	1 cut(s)	693
GlaI	GCGC	1 cut(s)	693
GluI	GCNGC	7 cut(s)	15, 95, 120, 321, 390, 393, 633
HaeIII	GGCC	2 cut(s)	63, 536
HapII	CCGG	1 cut(s)	596
HgaI	GACGC	1 cut(s)	233
HhaI	GCGC	1 cut(s)	694
Hin1II	CATG	6 cut(s)	119, 137, 301, 500, 534, 841
Hin6I	GCGC	1 cut(s)	692
HinP1I	GCGC	1 cut(s)	692
HincII	GTYRAC	1 cut(s)	795
HindII	GTYRAC	1 cut(s)	795
HinfI	GANTC	2 cut(s)	23, 140
HpaII	CCGG	1 cut(s)	596
Hpy166II	GTNNAC	2 cut(s)	378, 795
Hpy188I	TCNGA	4 cut(s)	22, 129, 766, 789
Hpy188III	TCNNGA	6 cut(s)	194, 221, 431, 596, 661, 911
Hpy8I	GTNNAC	2 cut(s)	378, 795
Hpy99I	CGWCG	1 cut(s)	227
HpyAV	CCTTC	1 cut(s)	134
HpyCH4III	ACNGT	3 cut(s)	112, 667, 728
HpyCH4IV	ACGT	1 cut(s)	330
HpyCH4V	TGCA	4 cut(s)	56, 97, 347, 500
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 69, 94, 243
HpyF3I	CTNAG	4 cut(s)	67, 84, 636, 682
HpySE526I	ACGT	1 cut(s)	330
Hsp92II	CATG	6 cut(s)	119, 137, 301, 500, 534, 841
HspAI	GCGC	1 cut(s)	692
Kpn2I	TCCGGA	1 cut(s)	595
Kzo9I	GATC	3 cut(s)	196, 412, 843
LmnI	GCTCC	4 cut(s)	552, 598, 629, 778
Lsp1109I	GCAGC	6 cut(s)	26, 81, 106, 332, 379, 644
LweI	GCATC	4 cut(s)	26, 43, 65, 679
MaeI	CTAG	2 cut(s)	335, 515
MaeII	ACGT	1 cut(s)	330
MaeIII	GTNAC	3 cut(s)	104, 164, 661
MalI	GATC	3 cut(s)	198, 414, 845
MboI	GATC	3 cut(s)	196, 412, 843
MboII	GAAGA	5 cut(s)	18, 375, 535, 659, 722
MflI	RGATCY	2 cut(s)	196, 843
MhlI	GDGCHC	2 cut(s)	90, 660
MluCI	AATT	4 cut(s)	469, 872, 881, 893
MlyI	GAGTC	1 cut(s)	17
MnlI	CCTC	5 cut(s)	89, 276, 304, 834, 858
Mph1103I	ATGCAT	2 cut(s)	58, 502
MroI	TCCGGA	1 cut(s)	595
MseI	TTAA	2 cut(s)	875, 897
MspA1I	CMGCKG	2 cut(s)	392, 635
MspI	CCGG	1 cut(s)	596
MspR9I	CCNGG	1 cut(s)	833
Mva1269I	GAATGC	1 cut(s)	216
MvaI	CCWGG	1 cut(s)	833
MvnI	CGCG	1 cut(s)	145
MwoI	GCNNNNNNNGC	4 cut(s)	11, 69, 94, 243
NdeII	GATC	3 cut(s)	196, 412, 843
NlaIII	CATG	6 cut(s)	119, 137, 301, 500, 534, 841
NlaIV	GGNNCC	5 cut(s)	6, 73, 198, 594, 845
NmuCI	GTSAC	2 cut(s)	164, 661
NsbI	TGCGCA	1 cut(s)	693
NsiI	ATGCAT	2 cut(s)	58, 502
NspI	RCATGY	1 cut(s)	500
NspV	TTCGAA	1 cut(s)	29
PaeI	GCATGC	1 cut(s)	500
PaqCI	CACCTGC	1 cut(s)	99
PcsI	WCGNNNNNNNCGW	1 cut(s)	26
PctI	GAATGC	1 cut(s)	216
PfeI	GAWTC	1 cut(s)	140
PflFI	GACNNNGTC	1 cut(s)	368
PkrI	GCNGC	7 cut(s)	16, 96, 121, 322, 391, 394, 634
Ple19I	CGATCG	1 cut(s)	415
PleI	GAGTC	1 cut(s)	17
PpsI	GAGTC	1 cut(s)	17
PshAI	GACNNNNGTC	1 cut(s)	464
Psp6I	CCWGG	1 cut(s)	831
PspGI	CCWGG	1 cut(s)	831
PspN4I	GGNNCC	5 cut(s)	6, 73, 198, 594, 845
PspPI	GGNCC	2 cut(s)	459, 534
PsuI	RGATCY	2 cut(s)	196, 843
PsyI	GACNNNGTC	1 cut(s)	368
PvuI	CGATCG	1 cut(s)	415
PvuII	CAGCTG	1 cut(s)	635
RsaI	GTAC	2 cut(s)	333, 644
RsaNI	GTAC	2 cut(s)	332, 643
SaqAI	TTAA	2 cut(s)	875, 897
SatI	GCNGC	7 cut(s)	15, 95, 120, 321, 390, 393, 633
Sau3AI	GATC	3 cut(s)	196, 412, 843
Sau96I	GGNCC	2 cut(s)	459, 534
SchI	GAGTC	1 cut(s)	17
ScrFI	CCNGG	1 cut(s)	833
SduI	GDGCHC	2 cut(s)	90, 660
SetI	ASST	8 cut(s)	40, 93, 180, 315, 333, 557, 637, 783
SfaNI	GCATC	4 cut(s)	26, 43, 65, 679
SfuI	TTCGAA	1 cut(s)	29
SinI	GGWCC	1 cut(s)	459
SmlI	CTYRAG	1 cut(s)	909
SmoI	CTYRAG	1 cut(s)	909
SphI	GCATGC	1 cut(s)	500
Sse9I	AATT	4 cut(s)	469, 872, 881, 893
SsiI	CCGC	3 cut(s)	145, 387, 390
SspMI	CTAG	2 cut(s)	335, 515
StyD4I	CCNGG	1 cut(s)	831
StyI	CCWWGG	1 cut(s)	537
TaaI	ACNGT	3 cut(s)	112, 667, 728
TaiI	ACGT	1 cut(s)	333
TaqI	TCGA	8 cut(s)	29, 204, 222, 316, 422, 687, 730, 850
TaqII	GACCGA	2 cut(s)	476, 750
TasI	AATT	4 cut(s)	469, 872, 881, 893
TauI	GCSGC	1 cut(s)	392
TfiI	GAWTC	1 cut(s)	140
Tru1I	TTAA	2 cut(s)	875, 897
Tru9I	TTAA	2 cut(s)	875, 897
TscAI	CASTG	1 cut(s)	461
TseFI	GTSAC	2 cut(s)	164, 661
TseI	GCWGC	6 cut(s)	14, 94, 119, 320, 392, 632
Tsp45I	GTSAC	2 cut(s)	164, 661
TspDTI	ATGAA	4 cut(s)	246, 306, 462, 801
TspGWI	ACGGA	1 cut(s)	189
TspRI	CASTG	1 cut(s)	461
Tth111I	GACNNNGTC	1 cut(s)	368
VpaK11BI	GGWCC	1 cut(s)	459
XapI	RAATTY	1 cut(s)	893
XceI	RCATGY	1 cut(s)	500
XspI	CTAG	2 cut(s)	335, 515
Zsp2I	ATGCAT	2 cut(s)	58, 502

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.