AT4G32140

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002838
Physical Location & Seq
Reverse (-)
15522788 .. 15525536
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32140.1

Sequence Viewer

Length: 1185 bp
ATGGGTTGGAGATACAAAGCTGGTCTGTTCTTGATTGGCACTGTTGTTATCATATGGGTCACCTCCGCTGAAGTTACTCAGGATATCTTTACAGCCTATAAGCAGCCATTTGCAGTCACATATCTTGGAGCTTCTCTTATGATTGTTTACCTTCCCGTTGCATTCCTCAAAGACTGGCTTTGTCGATATTTAGATCGACGCTCTTCCAAATCCAATAAAATTCCCGCTTTGACCGATGATTCCTCGGTTGAGCTCGGTTCTCCTTTGAGGCATAAAATCATTGAAATGGGACTTCAAGGGACTATAACTAAGAAAGATAGTGAGGAACACCTTTCATCCCATGAAGAAGAAGATGAGAGACCCTTGATCGGTAAAGTTAGAGAAGAAGTGCAGACTCTGAAACAGAGGAAAGAGATCACGACTAAGCAGATTGCAATGTACGGCCTTTACCTCGCACCAATTTGGTTTGTAACAGAGTATCTGTCCAATGCTGCTCTTGCTCGTACAAGTGTGGCAAGTACTACTGTATTGTCTTCAACCTCGGGACTGTTTACTCTGTTCATTGGTGTATTTTTGGGCCAAGATACCTTGAACCTGTCGAAAGTAGTGGCTGTATTTGTCAGCATGGCTGGTGTGGTCATGACGACGCTAGGGAAAACTTGGGCTTCTGATGAGTCGCAATTAAATTCTTCACTCAACGGGGAACGATCCCTTATGGGAGATCTTTTTGGGCTTCTCTCTGCGGTCTCTTATGGACTTTTTACAGTGCTTCTTAAGAAGTTTGCCGGTGAAGAAGGAGAAGGGGTTGATGTCCAAAAGCTGTTTGGATACATAGGATTATTTACACTTGTTGCTCTCTGGTGGCTTGTGTGGCCATTGACAGCATTAGGGATCGAACCAAAGTTTACCATACCACACTCTGTGAAAGTTGATGAAGTTGTCTTAGCCAACGGTTTCATCGGAAGTGTCCTCTCTGACTATTTTTGGGCACTTTCTGTGGTGTGGACAACTCCGCTGGTAGCGACACTGGGCATGTCTCTCACAATTCCGCTTGCGATGGTTGCTGACATGATGATCCATGGTCGTCATTATTCAGCCATTTACATTCTTGGCTCTACTCAGGTGTTTGCTGGATTCGTAATAGCTAACATATCGGATTTGTTCTCAAAGAAGCTTGGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

394

Amino Acids

43.4

Weight (kDa)

6.11

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 135 - 216 9.3e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 66, 225, 743, 1013, 1049
AclWI GGATC 3 cut(s) 702, 899, 1069
AcoI YGGCCR 1 cut(s) 872
AcsI RAATTY 2 cut(s) 219, 685
AcuI CTGAAG 1 cut(s) 90
AdeI CACNNNGTG 1 cut(s) 922
AfaI GTAC 3 cut(s) 440, 505, 520
AfiI CCNNNNNNNGG 1 cut(s) 368
AflII CTTAAG 1 cut(s) 773
AgsI TTSAA 4 cut(s) 284, 296, 537, 592
AluBI AGCT 6 cut(s) 20, 131, 253, 820, 1145, 1174
AluI AGCT 6 cut(s) 20, 131, 253, 820, 1145, 1174
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 3 cut(s) 352, 751, 1041
AlwI GGATC 3 cut(s) 702, 899, 1069
AlwNI CAGNNNCTG 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 541
AoxI GGCC 3 cut(s) 442, 577, 872
ApeKI GCWGC 2 cut(s) 103, 491
ApoI RAATTY 2 cut(s) 219, 685
AspS9I GGNCC 1 cut(s) 577
AsuHPI GGTGA 2 cut(s) 52, 800
AvaI CYCGRG 1 cut(s) 541
BaeGI GKGCMC 1 cut(s) 991
BalI TGGCCA 1 cut(s) 874
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 115, 478
BccI CCATC 1 cut(s) 1051
BceAI ACGGC 1 cut(s) 457
BciVI GTATCC 1 cut(s) 821
BcoDI GTCTC 3 cut(s) 352, 751, 1041
BfaI CTAG 1 cut(s) 650
BfrI CTTAAG 1 cut(s) 773
BfuI GTATCC 1 cut(s) 821
BglII AGATCT 1 cut(s) 721
BisI GCNGC 2 cut(s) 104, 492
BlsI GCNGC 2 cut(s) 105, 493
BmcAI AGTACT 1 cut(s) 520
BmeT110I CYCGRG 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 577
BmrI ACTGGG 1 cut(s) 1037
BmuI ACTGGG 1 cut(s) 1037
BpiI GAAGAC 1 cut(s) 525
BsaI GGTCTC 2 cut(s) 352, 751
BsaJI CCNNGG 3 cut(s) 243, 540, 1078
BsaXI ACNNNNNCTCC 2 cut(s) 789, 819
Bsc4I CCNNNNNNNGG 1 cut(s) 368
Bse118I RCCGGY 1 cut(s) 785
Bse1I ACTGG 2 cut(s) 179, 1032
Bse3DI GCAATG 1 cut(s) 441
BseDI CCNNGG 3 cut(s) 243, 540, 1078
BseGI GGATG 1 cut(s) 335
BseLI CCNNNNNNNGG 1 cut(s) 368
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 2 cut(s) 92, 1133
BseNI ACTGG 2 cut(s) 179, 1032
BseSI GKGCMC 1 cut(s) 991
BseXI GCAGC 2 cut(s) 115, 478
BsgI GTGCAG 1 cut(s) 410
BshFI GGCC 3 cut(s) 444, 579, 874
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 1 cut(s) 786
BslFI GGGAC 3 cut(s) 303, 313, 558
BslI CCNNNNNNNGG 1 cut(s) 368
BsmAI GTCTC 3 cut(s) 352, 751, 1041
BsmFI GGGAC 3 cut(s) 303, 313, 558
BsmI GAATGC 1 cut(s) 161
BsnI GGCC 3 cut(s) 444, 579, 874
Bso31I GGTCTC 2 cut(s) 352, 751
BsoBI CYCGRG 1 cut(s) 541
Bsp1286I GDGCHC 2 cut(s) 255, 991
Bsp143I GATC 7 cut(s) 193, 366, 414, 707, 721, 891, 1074
Bsp19I CCATGG 1 cut(s) 1078
BspACI CCGC 5 cut(s) 66, 225, 743, 1013, 1049
BspANI GGCC 3 cut(s) 444, 579, 874
BspCNI CTCAG 2 cut(s) 91, 1132
BspHI TCATGA 1 cut(s) 639
BspPI GGATC 3 cut(s) 702, 899, 1069
BspQI GCTCTTC 1 cut(s) 208
BspTI CTTAAG 1 cut(s) 773
BspTNI GGTCTC 2 cut(s) 352, 751
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 785
BsrI ACTGG 2 cut(s) 179, 1032
BssAI RCCGGY 1 cut(s) 785
BssECI CCNNGG 3 cut(s) 243, 540, 1078
BssMI GATC 7 cut(s) 193, 366, 414, 707, 721, 891, 1074
BssT1I CCWWGG 1 cut(s) 1078
Bst4CI ACNGT 5 cut(s) 43, 526, 549, 766, 953
Bst6I CTCTTC 1 cut(s) 208
BstAFI CTTAAG 1 cut(s) 773
BstC8I GCNNGC 1 cut(s) 1053
BstDEI CTNAG 5 cut(s) 78, 309, 423, 943, 1119
BstDSI CCRYGG 1 cut(s) 1078
BstEII GGTNACC 1 cut(s) 58
BstF5I GGATG 1 cut(s) 335
BstKTI GATC 7 cut(s) 196, 369, 417, 710, 724, 894, 1077
BstMAI GTCTC 3 cut(s) 352, 751, 1041
BstMBI GATC 7 cut(s) 193, 366, 414, 707, 721, 891, 1074
BstMWI GCNNNNNNNGC 3 cut(s) 497, 871, 1061
BstNSI RCATGY 1 cut(s) 1036
BstPI GGTNACC 1 cut(s) 58
BstSLI GKGCMC 1 cut(s) 991
BstV1I GCAGC 2 cut(s) 115, 478
BstV2I GAAGAC 1 cut(s) 525
BstX2I RGATCY 1 cut(s) 721
BstYI RGATCY 1 cut(s) 721
BsuI GTATCC 1 cut(s) 821
BsuRI GGCC 3 cut(s) 444, 579, 874
BtgI CCRYGG 1 cut(s) 1078
BtgZI GCGATG 1 cut(s) 1070
BtsCI GGATG 1 cut(s) 335
BtsIMutI CAGTG 3 cut(s) 39, 771, 1025
Cac8I GCNNGC 1 cut(s) 1053
CaiI CAGNNNCTG 1 cut(s) 397
CciI TCATGA 1 cut(s) 639
Cfr10I RCCGGY 1 cut(s) 785
Cfr13I GGNCC 1 cut(s) 577
CseI GACGC 2 cut(s) 207, 655
Csp6I GTAC 3 cut(s) 439, 504, 519
CviAII CATG 6 cut(s) 341, 625, 640, 1033, 1069, 1079
CviQI GTAC 3 cut(s) 439, 504, 519
DdeI CTNAG 5 cut(s) 78, 309, 423, 943, 1119
DpnI GATC 7 cut(s) 195, 368, 416, 709, 723, 893, 1076
DpnII GATC 7 cut(s) 193, 366, 414, 707, 721, 891, 1074
DraIII CACNNNGTG 1 cut(s) 922
EaeI YGGCCR 1 cut(s) 872
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 1078
Eco24I GRGCYC 1 cut(s) 255
Eco31I GGTCTC 2 cut(s) 352, 751
Eco32I GATATC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 541
Eco91I GGTNACC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 58
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 1078
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 1078
FaeI CATG 6 cut(s) 344, 628, 643, 1036, 1072, 1082
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FaqI GGGAC 3 cut(s) 303, 313, 558
FatI CATG 6 cut(s) 340, 624, 639, 1032, 1068, 1078
FauI CCCGC 1 cut(s) 232
FauNDI CATATG 1 cut(s) 53
Fnu4HI GCNGC 2 cut(s) 104, 492
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 2 cut(s) 104, 492
FspBI CTAG 1 cut(s) 650
GluI GCNGC 2 cut(s) 104, 492
HaeIII GGCC 3 cut(s) 444, 579, 874
HapII CCGG 1 cut(s) 786
HgaI GACGC 2 cut(s) 207, 655
Hin1II CATG 6 cut(s) 344, 628, 643, 1036, 1072, 1082
HindIII AAGCTT 1 cut(s) 1172
HinfI GANTC 4 cut(s) 239, 394, 674, 1134
HpaII CCGG 1 cut(s) 786
HphI GGTGA 2 cut(s) 52, 800
Hpy166II GTNNAC 4 cut(s) 148, 552, 906, 1005
Hpy188I TCNGA 5 cut(s) 399, 670, 962, 976, 1156
Hpy188III TCNNGA 5 cut(s) 31, 80, 418, 543, 640
Hpy8I GTNNAC 4 cut(s) 148, 552, 906, 1005
Hpy99I CGWCG 2 cut(s) 201, 649
HpyAV CCTTC 3 cut(s) 161, 788, 794
HpyCH4III ACNGT 5 cut(s) 43, 526, 549, 766, 953
HpyCH4V TGCA 4 cut(s) 113, 161, 391, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 497, 871, 1061
HpyF3I CTNAG 5 cut(s) 78, 309, 423, 943, 1119
Hsp92II CATG 6 cut(s) 344, 628, 643, 1036, 1072, 1082
Kzo9I GATC 7 cut(s) 193, 366, 414, 707, 721, 891, 1074
LguI GCTCTTC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 2 cut(s) 115, 478
MaeI CTAG 1 cut(s) 650
MaeIII GTNAC 4 cut(s) 58, 73, 115, 469
MalI GATC 7 cut(s) 195, 368, 416, 709, 723, 893, 1076
MboI GATC 7 cut(s) 193, 366, 414, 707, 721, 891, 1074
MboII GAAGA 8 cut(s) 195, 356, 359, 362, 395, 525, 681, 803
MflI RGATCY 1 cut(s) 721
MhlI GDGCHC 2 cut(s) 255, 991
MlsI TGGCCA 1 cut(s) 874
MluCI AATT 5 cut(s) 219, 459, 680, 685, 1044
MluNI TGGCCA 1 cut(s) 874
MlyI GAGTC 2 cut(s) 388, 683
MnlI CCTC 9 cut(s) 73, 176, 253, 261, 316, 399, 461, 550, 980
Mox20I TGGCCA 1 cut(s) 874
MscI TGGCCA 1 cut(s) 874
MseI TTAA 2 cut(s) 683, 774
MslI CAYNNNNRTG 1 cut(s) 284
Msp20I TGGCCA 1 cut(s) 874
MspA1I CMGCKG 2 cut(s) 68, 1015
MspCI CTTAAG 1 cut(s) 773
MspI CCGG 1 cut(s) 786
Mva1269I GAATGC 1 cut(s) 161
MwoI GCNNNNNNNGC 3 cut(s) 497, 871, 1061
NcoI CCATGG 1 cut(s) 1078
NdeI CATATG 1 cut(s) 53
NdeII GATC 7 cut(s) 193, 366, 414, 707, 721, 891, 1074
NlaIII CATG 6 cut(s) 344, 628, 643, 1036, 1072, 1082
NmuCI GTSAC 2 cut(s) 58, 115
NspI RCATGY 1 cut(s) 1036
PagI TCATGA 1 cut(s) 639
PciSI GCTCTTC 1 cut(s) 208
PctI GAATGC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 239, 1134
PkrI GCNGC 2 cut(s) 105, 493
PleI GAGTC 2 cut(s) 388, 682
PpsI GAGTC 2 cut(s) 388, 682
Psp124BI GAGCTC 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 58
PspPI GGNCC 1 cut(s) 577
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 1 cut(s) 721
RsaI GTAC 3 cut(s) 440, 505, 520
RsaNI GTAC 3 cut(s) 439, 504, 519
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 2 cut(s) 683, 774
SatI GCNGC 2 cut(s) 104, 492
Sau3AI GATC 7 cut(s) 193, 366, 414, 707, 721, 891, 1074
Sau96I GGNCC 1 cut(s) 577
ScaI AGTACT 1 cut(s) 520
SchI GAGTC 2 cut(s) 388, 683
SduI GDGCHC 2 cut(s) 255, 991
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
Sse9I AATT 5 cut(s) 219, 459, 680, 685, 1044
SsiI CCGC 5 cut(s) 66, 225, 743, 1013, 1049
SspMI CTAG 1 cut(s) 650
SstI GAGCTC 1 cut(s) 255
StyI CCWWGG 1 cut(s) 1078
TaaI ACNGT 5 cut(s) 43, 526, 549, 766, 953
TaqI TCGA 4 cut(s) 184, 196, 599, 894
TaqII GACCGA 1 cut(s) 248
TasI AATT 5 cut(s) 219, 459, 680, 685, 1044
TatI WGTACW 1 cut(s) 518
TfiI GAWTC 2 cut(s) 239, 1134
Tru1I TTAA 2 cut(s) 683, 774
Tru9I TTAA 2 cut(s) 683, 774
TscAI CASTG 3 cut(s) 46, 771, 1032
TseFI GTSAC 2 cut(s) 58, 115
TseI GCWGC 2 cut(s) 103, 491
Tsp45I GTSAC 2 cut(s) 58, 115
TspDTI ATGAA 5 cut(s) 324, 357, 550, 946, 948
TspRI CASTG 3 cut(s) 46, 771, 1032
Vha464I CTTAAG 1 cut(s) 773
XapI RAATTY 2 cut(s) 219, 685
XceI RCATGY 1 cut(s) 1036
XcmI CCANNNNNNNNNTGG 1 cut(s) 821
XspI CTAG 1 cut(s) 650
ZrmI AGTACT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.