Rw6G034800

Solute carrier family 35 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002838
Physical Location & Seq
Forward (+)
59444178 .. 59447312
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034800.1

Sequence Viewer

Length: 1113 bp
ATGGGTTGGAAATACAACGTAGGATTGGGGTTGATTGGTGCCGTCGTCTTCATATGGGTCGCCTCTGCAGAGGTTACTCAGAGAATTTTCACAGAGTACAAACAGCCTTTTTCACTCACCTATCTCGGTGTGTCCCTCATGGTCGTCTATCTACCTATAGCAGCCCTCAAGGACTGGATCTGCAGCTTACTGAGAAAAAATGAATTTCATGACAGTGCAGAAACAGATGTCACAACTTCCTTAATTACTGATAAAGACCTCAGTGATAGAGAAGAGGGGCGCCCTGTAATGGATAAGAATGACGTAGATGAGCCTTACTTTCTTGCAAAAAGTAATCAACTTAGTCCATGGGAAATTGCAAAATTTGGCTTAATACTTACTCCAGCATGGTTTATCACAGAGTATTTGTCAAATTCTGCCCTGGCAAATACTAGTGTTGCAAGTACAACTGTCCTAACTTCCACATCGGGGCTCTTTACACTTTTGTTTGGAGCACTTCTTCGTCAGGACTCCATAAACATTGCAAAGATGGTTGCTGTTTTTATCAGCATGGCTGGTGTTGCCATGACCACAGCTGGAAAAACTTGGGCACCTGATGAACTGCTGAGCGTCTCTGAGACTAGAAAGCACTCAATAACAGGGGACATTTTCGGTCTTGTGTCAGCAGCATCATATGGATTATTCACAGTGTTACTCAAGAAATTTGCTGGATCAGAAGAGGAAGGGGATAAGGCTGATGTGCAAAAGCTATTCGGATACATTGGTCTCTTCACTCTTCTTGGTCTGTGGTGGCTTGTGTTGCCGCTGAATGCTATGGGGATTGAACCGTCTTTTGAATATCCACCATCAGGATCTGTTGGGGAAGCTGTGCTGTTAAATGGCTTTGTGGGGAGTGTTCTTTCAGATTATTTTTGGGCTCTTTCTGTAGTTTGGACATCTCCGTTAGTTGCAACTTTGGGCATGTCCTTGACAATTCCCCTTGCTATGGTTGCTGACATGGTGATTCACGGCCGTCGTTACTCTGCAATCTACATCTCTGGTTGCATTCAGGTTTTTGCAGGATTTGTCATGGCAAACCTTTCAAACAAGTTTTCTAGCAAGGAAGAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

370

Amino Acids

40.4

Weight (kDa)

4.88

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 109 - 191 5.2e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 38, 279, 589
AciI CCGC 1 cut(s) 803
AclWI GGATC 3 cut(s) 185, 718, 859
AcoI YGGCCR 1 cut(s) 1009
AcsI RAATTY 5 cut(s) 84, 203, 362, 412, 701
AcyI GRCGYC 1 cut(s) 280
AfaI GTAC 2 cut(s) 98, 445
AfiI CCNNNNNNNGG 4 cut(s) 289, 468, 848, 985
AgsI TTSAA 3 cut(s) 824, 836, 1083
AhlI ACTAGT 1 cut(s) 431
AjnI CCWGG 1 cut(s) 420
AluBI AGCT 4 cut(s) 186, 575, 748, 866
AluI AGCT 4 cut(s) 186, 575, 748, 866
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 3 cut(s) 611, 616, 770
AlwI GGATC 3 cut(s) 185, 718, 859
AlwNI CAGNNNCTG 1 cut(s) 854
AoxI GGCC 1 cut(s) 1009
ApeKI GCWGC 3 cut(s) 161, 183, 665
ApoI RAATTY 5 cut(s) 84, 203, 362, 412, 701
AspLEI GCGC 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 109, 1012
BaeGI GKGCMC 1 cut(s) 592
BaeI ACNNNNGTAYC 2 cut(s) 748, 781
BanI GGYRCC 3 cut(s) 38, 279, 589
BanII GRGCYC 2 cut(s) 474, 919
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 3 cut(s) 173, 195, 677
BccI CCATC 2 cut(s) 523, 853
BceAI ACGGC 3 cut(s) 26, 996, 1024
BciT130I CCWGG 1 cut(s) 422
BciVI GTATCC 1 cut(s) 749
BcoDI GTCTC 3 cut(s) 611, 616, 770
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 3 cut(s) 432, 621, 1095
BfmI CTRYAG 4 cut(s) 66, 156, 181, 924
BfoI RGCGCY 1 cut(s) 283
BfuI GTATCC 1 cut(s) 749
BisI GCNGC 4 cut(s) 162, 184, 666, 803
BlpI GCTNAGC 1 cut(s) 605
BlsI GCNGC 4 cut(s) 163, 185, 667, 804
Bme1390I CCNGG 1 cut(s) 422
BmiI GGNNCC 3 cut(s) 40, 281, 591
BmrFI CCNGG 1 cut(s) 422
BmsI GCATC 1 cut(s) 677
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 605
BpuEI CTTGAG 2 cut(s) 152, 680
BsaHI GRCGYC 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 770
BsaJI CCNNGG 2 cut(s) 347, 420
Bsc4I CCNNNNNNNGG 4 cut(s) 289, 468, 848, 985
Bse1I ACTGG 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 347, 420
BseLI CCNNNNNNNGG 4 cut(s) 289, 468, 848, 985
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 5 cut(s) 92, 182, 274, 596, 606
BseNI ACTGG 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 592
BseX3I CGGCCG 1 cut(s) 1009
BseXI GCAGC 3 cut(s) 173, 195, 677
BsgI GTGCAG 1 cut(s) 237
Bsh1285I CGRYCG 1 cut(s) 1012
BshFI GGCC 1 cut(s) 1011
BshNI GGYRCC 3 cut(s) 38, 279, 589
BsiEI CGRYCG 1 cut(s) 1012
BsiHKAI GWGCWC 1 cut(s) 496
BslFI GGGAC 2 cut(s) 118, 656
BslI CCNNNNNNNGG 4 cut(s) 289, 468, 848, 985
BsmAI GTCTC 3 cut(s) 611, 616, 770
BsmBI CGTCTC 1 cut(s) 616
BsmFI GGGAC 2 cut(s) 118, 656
BsmI GAATGC 2 cut(s) 814, 1044
BsnI GGCC 1 cut(s) 1011
Bso31I GGTCTC 1 cut(s) 770
Bsp1286I GDGCHC 4 cut(s) 474, 496, 592, 919
Bsp143I GATC 3 cut(s) 177, 710, 851
Bsp1720I GCTNAGC 1 cut(s) 605
Bsp19I CCATGG 1 cut(s) 347
BspACI CCGC 1 cut(s) 803
BspANI GGCC 1 cut(s) 1011
BspCNI CTCAG 5 cut(s) 91, 183, 273, 597, 607
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 3 cut(s) 40, 281, 591
BspMAI CTGCAG 2 cut(s) 70, 185
BspPI GGATC 3 cut(s) 185, 718, 859
BspT107I GGYRCC 3 cut(s) 38, 279, 589
BspTNI GGTCTC 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 347, 420
BssMI GATC 3 cut(s) 177, 710, 851
BssNI GRCGYC 1 cut(s) 280
BssT1I CCWWGG 1 cut(s) 347
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 4 cut(s) 215, 451, 688, 828
Bst6I CTCTTC 5 cut(s) 267, 711, 773, 780, 1098
BstACI GRCGYC 1 cut(s) 280
BstDEI CTNAG 6 cut(s) 78, 191, 260, 341, 605, 615
BstDSI CCRYGG 1 cut(s) 347
BstH2I RGCGCY 1 cut(s) 283
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 3 cut(s) 180, 713, 854
BstMAI GTCTC 3 cut(s) 611, 616, 770
BstMBI GATC 3 cut(s) 177, 710, 851
BstMCI CGRYCG 1 cut(s) 1012
BstMWI GCNNNNNNNGC 3 cut(s) 560, 799, 989
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 4 cut(s) 66, 156, 181, 924
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 3 cut(s) 173, 195, 677
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 2 cut(s) 177, 851
BstYI RGATCY 2 cut(s) 177, 851
BstZI CGGCCG 1 cut(s) 1009
BsuI GTATCC 1 cut(s) 749
BsuRI GGCC 1 cut(s) 1011
BtgI CCRYGG 1 cut(s) 347
BtsIMutI CAGTG 3 cut(s) 220, 268, 693
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 1 cut(s) 208
CfoI GCGC 1 cut(s) 282
CseI GACGC 1 cut(s) 598
Csp6I GTAC 2 cut(s) 97, 444
CviAII CATG 9 cut(s) 139, 209, 348, 387, 550, 565, 961, 997, 1069
CviQI GTAC 2 cut(s) 97, 444
DdeI CTNAG 6 cut(s) 78, 191, 260, 341, 605, 615
DinI GGCGCC 1 cut(s) 281
DpnI GATC 3 cut(s) 179, 712, 853
DpnII GATC 3 cut(s) 177, 710, 851
EaeI YGGCCR 1 cut(s) 1009
EagI CGGCCG 1 cut(s) 1009
Eam1104I CTCTTC 5 cut(s) 267, 711, 773, 780, 1098
EarI CTCTTC 5 cut(s) 267, 711, 773, 780, 1098
EclXI CGGCCG 1 cut(s) 1009
Eco130I CCWWGG 1 cut(s) 347
Eco24I GRGCYC 2 cut(s) 474, 919
Eco31I GGTCTC 1 cut(s) 770
Eco52I CGGCCG 1 cut(s) 1009
EcoRII CCWGG 1 cut(s) 420
EcoT14I CCWWGG 1 cut(s) 347
EcoT38I GRGCYC 2 cut(s) 474, 919
EgeI GGCGCC 1 cut(s) 281
EheI GGCGCC 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 347
Esp3I CGTCTC 1 cut(s) 616
FaeI CATG 9 cut(s) 142, 212, 351, 390, 553, 568, 964, 1000, 1072
FaqI GGGAC 2 cut(s) 118, 656
FatI CATG 9 cut(s) 138, 208, 347, 386, 549, 564, 960, 996, 1068
FauNDI CATATG 2 cut(s) 53, 673
Fnu4HI GCNGC 4 cut(s) 162, 184, 666, 803
FriOI GRGCYC 2 cut(s) 474, 919
Fsp4HI GCNGC 4 cut(s) 162, 184, 666, 803
FspBI CTAG 3 cut(s) 432, 621, 1095
GlaI GCGC 1 cut(s) 281
GluI GCNGC 4 cut(s) 162, 184, 666, 803
GsuI CTGGAG 1 cut(s) 366
HaeII RGCGCY 1 cut(s) 283
HaeIII GGCC 1 cut(s) 1011
HgaI GACGC 1 cut(s) 598
HhaI GCGC 1 cut(s) 282
Hin1I GRCGYC 1 cut(s) 280
Hin1II CATG 9 cut(s) 142, 212, 351, 390, 553, 568, 964, 1000, 1072
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HinfI GANTC 2 cut(s) 509, 1003
HphI GGTGA 2 cut(s) 109, 1012
Hpy188I TCNGA 5 cut(s) 81, 616, 715, 755, 904
Hpy188III TCNNGA 4 cut(s) 209, 506, 697, 849
Hpy99I CGWCG 2 cut(s) 47, 1017
HpyAV CCTTC 1 cut(s) 716
HpyCH4III ACNGT 4 cut(s) 215, 451, 688, 828
HpyCH4IV ACGT 2 cut(s) 18, 303
HpyF10VI GCNNNNNNNGC 3 cut(s) 560, 799, 989
HpyF3I CTNAG 6 cut(s) 78, 191, 260, 341, 605, 615
HpySE526I ACGT 2 cut(s) 18, 303
Hsp92I GRCGYC 1 cut(s) 280
Hsp92II CATG 9 cut(s) 142, 212, 351, 390, 553, 568, 964, 1000, 1072
HspAI GCGC 1 cut(s) 280
KasI GGCGCC 1 cut(s) 279
Kzo9I GATC 3 cut(s) 177, 710, 851
LmnI GCTCC 1 cut(s) 491
Lsp1109I GCAGC 3 cut(s) 173, 195, 677
LweI GCATC 1 cut(s) 677
MaeI CTAG 3 cut(s) 432, 621, 1095
MaeII ACGT 2 cut(s) 18, 303
MaeIII GTNAC 4 cut(s) 73, 229, 690, 1016
MalI GATC 3 cut(s) 179, 712, 853
MboI GATC 3 cut(s) 177, 710, 851
MboII GAAGA 6 cut(s) 40, 284, 491, 728, 760, 767
MflI RGATCY 2 cut(s) 177, 851
MhlI GDGCHC 4 cut(s) 474, 496, 592, 919
MluCI AATT 8 cut(s) 84, 203, 243, 354, 362, 412, 701, 972
Mly113I GGCGCC 1 cut(s) 280
MlyI GAGTC 1 cut(s) 503
MnlI CCTC 7 cut(s) 64, 73, 146, 176, 268, 269, 712
MseI TTAA 3 cut(s) 242, 371, 875
MslI CAYNNNNRTG 1 cut(s) 213
MspA1I CMGCKG 2 cut(s) 575, 805
MspR9I CCNGG 1 cut(s) 422
Mva1269I GAATGC 2 cut(s) 814, 1044
MvaI CCWGG 1 cut(s) 422
MwoI GCNNNNNNNGC 3 cut(s) 560, 799, 989
NarI GGCGCC 1 cut(s) 280
NcoI CCATGG 1 cut(s) 347
NdeI CATATG 2 cut(s) 53, 673
NdeII GATC 3 cut(s) 177, 710, 851
NlaIII CATG 9 cut(s) 142, 212, 351, 390, 553, 568, 964, 1000, 1072
NlaIV GGNNCC 3 cut(s) 40, 281, 591
NmuCI GTSAC 1 cut(s) 229
NspI RCATGY 1 cut(s) 964
PagI TCATGA 1 cut(s) 208
PctI GAATGC 2 cut(s) 814, 1044
PfeI GAWTC 1 cut(s) 1003
PkrI GCNGC 4 cut(s) 163, 185, 667, 804
PleI GAGTC 1 cut(s) 503
PluTI GGCGCC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 3 cut(s) 40, 281, 591
PstI CTGCAG 2 cut(s) 70, 185
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 2 cut(s) 177, 851
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 2 cut(s) 98, 445
RsaNI GTAC 2 cut(s) 97, 444
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 3 cut(s) 242, 371, 875
SatI GCNGC 4 cut(s) 162, 184, 666, 803
Sau3AI GATC 3 cut(s) 177, 710, 851
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 4 cut(s) 474, 496, 592, 919
SfaNI GCATC 1 cut(s) 677
SfcI CTRYAG 4 cut(s) 66, 156, 181, 924
SfoI GGCGCC 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 2 cut(s) 167, 695
SmoI CTYRAG 2 cut(s) 167, 695
SpeI ACTAGT 1 cut(s) 431
Sse9I AATT 8 cut(s) 84, 203, 243, 354, 362, 412, 701, 972
SsiI CCGC 1 cut(s) 803
SspDI GGCGCC 1 cut(s) 279
SspMI CTAG 3 cut(s) 432, 621, 1095
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 4 cut(s) 215, 451, 688, 828
TaiI ACGT 2 cut(s) 21, 306
TaqII GACCGA 1 cut(s) 641
TasI AATT 8 cut(s) 84, 203, 243, 354, 362, 412, 701, 972
TatI WGTACW 2 cut(s) 96, 443
TauI GCSGC 1 cut(s) 805
TfiI GAWTC 1 cut(s) 1003
Tru1I TTAA 3 cut(s) 242, 371, 875
Tru9I TTAA 3 cut(s) 242, 371, 875
TscAI CASTG 3 cut(s) 220, 268, 693
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 3 cut(s) 161, 183, 665
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 4 cut(s) 40, 197, 216, 612
TspGWI ACGGA 1 cut(s) 930
TspRI CASTG 3 cut(s) 220, 268, 693
XapI RAATTY 5 cut(s) 84, 203, 362, 412, 701
XceI RCATGY 1 cut(s) 964
XspI CTAG 3 cut(s) 432, 621, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.