Rh6AG384400

Vacuolar membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002838
Physical Location & Seq
Reverse (-)
58389602 .. 58393801
4200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG384400.1

Sequence Viewer

Length: 885 bp
ATGGGTTGGAGATACAGAGCTGGGTTGCTTCTAATTGGTGCTGTGGTTATCATCTGGGTTACCTCTGCAGAAGTCACACAAGATATCTATGAAAAGTATAAGCAGCCATTTGCAGTAACATATCTTGGAGCTTCTCTTATGGTAGTGTACCTCCCAATAGCTTTCCTTAAGGACTGGTTGTGTAATTTACTTAAACGTCACTCTTCTAAAAGTGGCAAAAATGCAGAAAGCATGAATGAGTATTCTGCTGGATTTAGTTCACCACTAAAACACAATGGAGTGCAGAAAGATTTTGAATTGGAAATTCATACCTTGTCCAGAAAAGACAGTGATGCAGACCTTTCACCTTGTGCAGAAGAAAGGCCTTTGGTTTCTAAACTCAAAGACAATTTAAATGTGCTGAAACATGACAGAGAGCTCACACCAAGGGAAATTGCTACTTATGGATTTTACCTTGCCCCTCTTTGGTTTTTTACAGAGTATTTTGCAAATGCTGCTCTCGCGCGTACGAGTGTTGCAAGTACAACAGTCTTATCCTCAACTTCAGGACTCTTTACACTCTTTATTGGTGCATTCTTGGGCCAAGATTCTTTAAATATAGCAAAAGTGGTGGCTGTCTTTGTCAGCATGGCGGGTGTAGCCATGACAACTATGGGAAAAACATGGGCTACAGATGAGTCACAATCGAGTGCCTCCAATGGGAAACACTCTCTCGTTGGAGATCTTTTTGGCCTTTCCTCAGCTATGTCATACGGTCTATTTACAGTCCTTCTTAAGAAATTTGGTGGTGAAGGAGGAGAGAAGGCTGACGTGCAAAAATTGTTTGGGTATATTGGATTGTTTACACTCGTAGCATTATGGTGGCTGGGTAAGTATTGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

294

Amino Acids

32.38

Weight (kDa)

8.28

Isoelectric Point (pI)

28.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 140 - 217 1.1e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 503, 505
AciI CCGC 1 cut(s) 632
AcsI RAATTY 2 cut(s) 303, 779
AcuI CTGAAG 1 cut(s) 528
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 3 cut(s) 149, 508, 523
AfiI CCNNNNNNNGG 2 cut(s) 465, 699
AflII CTTAAG 2 cut(s) 167, 773
AgsI TTSAA 1 cut(s) 296
AjiI CACGTC 1 cut(s) 811
AluBI AGCT 5 cut(s) 20, 131, 161, 418, 743
AluI AGCT 5 cut(s) 20, 131, 161, 418, 743
Alw21I GWGCWC 1 cut(s) 420
AoxI GGCC 3 cut(s) 362, 580, 730
ApeKI GCWGC 2 cut(s) 103, 494
ApoI RAATTY 2 cut(s) 303, 779
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 580
AsuHPI GGTGA 3 cut(s) 252, 336, 800
BanII GRGCYC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 420
BbvCI CCTCAGC 1 cut(s) 739
BbvI GCAGC 2 cut(s) 115, 481
BfmI CTRYAG 2 cut(s) 66, 669
BfrI CTTAAG 2 cut(s) 167, 773
BglII AGATCT 1 cut(s) 721
BisI GCNGC 2 cut(s) 104, 495
BlsI GCNGC 2 cut(s) 105, 496
BmgBI CACGTC 1 cut(s) 811
BmgT120I GGNCC 1 cut(s) 580
BmsI GCATC 1 cut(s) 322
Bpu10I CCTNAGC 1 cut(s) 739
BsaJI CCNNGG 1 cut(s) 425
Bsc4I CCNNNNNNNGG 2 cut(s) 465, 699
Bse1I ACTGG 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 425
BseLI CCNNNNNNNGG 2 cut(s) 465, 699
BseMII CTCAG 1 cut(s) 753
BseNI ACTGG 1 cut(s) 179
BseRI GAGGAG 1 cut(s) 810
BseXI GCAGC 2 cut(s) 115, 481
BseYI CCCAGC 2 cut(s) 20, 865
BsgI GTGCAG 2 cut(s) 302, 372
Bsh1236I CGCG 2 cut(s) 503, 505
BshFI GGCC 3 cut(s) 364, 582, 732
BsiHKAI GWGCWC 1 cut(s) 420
BsiWI CGTACG 1 cut(s) 506
BslI CCNNNNNNNGG 2 cut(s) 465, 699
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 3 cut(s) 364, 582, 732
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 1 cut(s) 721
BspACI CCGC 1 cut(s) 632
BspANI GGCC 3 cut(s) 364, 582, 732
BspCNI CTCAG 1 cut(s) 752
BspFNI CGCG 2 cut(s) 503, 505
BspMAI CTGCAG 1 cut(s) 70
BspTI CTTAAG 2 cut(s) 167, 773
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 425
BssMI GATC 1 cut(s) 721
BssT1I CCWWGG 1 cut(s) 425
Bst4CI ACNGT 4 cut(s) 329, 529, 755, 766
Bst6I CTCTTC 1 cut(s) 208
BstAFI CTTAAG 2 cut(s) 167, 773
BstAPI GCANNNNNTGC 1 cut(s) 494
BstDEI CTNAG 1 cut(s) 739
BstEII GGTNACC 1 cut(s) 58
BstFNI CGCG 2 cut(s) 503, 505
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 1 cut(s) 724
BstMBI GATC 1 cut(s) 721
BstMWI GCNNNNNNNGC 3 cut(s) 494, 500, 638
BstPI GGTNACC 1 cut(s) 58
BstSFI CTRYAG 2 cut(s) 66, 669
BstUI CGCG 2 cut(s) 503, 505
BstV1I GCAGC 2 cut(s) 115, 481
BstX2I RGATCY 1 cut(s) 721
BstYI RGATCY 1 cut(s) 721
BsuRI GGCC 3 cut(s) 364, 582, 732
BtrI CACGTC 1 cut(s) 811
BtsIMutI CAGTG 1 cut(s) 334
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 580
Csp6I GTAC 3 cut(s) 148, 507, 522
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 5 cut(s) 232, 407, 628, 643, 663
CviQI GTAC 3 cut(s) 148, 507, 522
DdeI CTNAG 1 cut(s) 739
DpnI GATC 1 cut(s) 723
DpnII GATC 1 cut(s) 721
DraI TTTAAA 2 cut(s) 393, 594
DraIII CACNNNGTG 1 cut(s) 350
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 425
Eco147I AGGCCT 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 420
Eco32I GATATC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 418
EcoO65I GGTNACC 1 cut(s) 58
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 425
FaeI CATG 5 cut(s) 235, 410, 631, 646, 666
FalI AAGNNNNNCTT 1 cut(s) 863
FatI CATG 5 cut(s) 231, 406, 627, 642, 662
FauI CCCGC 1 cut(s) 625
Fnu4HI GCNGC 2 cut(s) 104, 495
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 2 cut(s) 104, 495
GlaI GCGC 1 cut(s) 504
GluI GCNGC 2 cut(s) 104, 495
GsaI CCCAGC 2 cut(s) 24, 869
HaeIII GGCC 3 cut(s) 364, 582, 732
HhaI GCGC 1 cut(s) 505
Hin1II CATG 5 cut(s) 235, 410, 631, 646, 666
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HinfI GANTC 3 cut(s) 549, 587, 677
HphI GGTGA 3 cut(s) 252, 336, 800
Hpy166II GTNNAC 3 cut(s) 148, 260, 843
Hpy188III TCNNGA 2 cut(s) 318, 546
Hpy8I GTNNAC 3 cut(s) 148, 260, 843
HpyAV CCTTC 3 cut(s) 779, 785, 796
HpyCH4III ACNGT 4 cut(s) 329, 529, 755, 766
HpyCH4IV ACGT 2 cut(s) 196, 810
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 500, 638
HpyF3I CTNAG 1 cut(s) 739
HpySE526I ACGT 2 cut(s) 196, 810
Hsp92II CATG 5 cut(s) 235, 410, 631, 646, 666
HspAI GCGC 1 cut(s) 503
Kzo9I GATC 1 cut(s) 721
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 7 cut(s) 6, 40, 160, 234, 331, 531, 851
Lsp1109I GCAGC 2 cut(s) 115, 481
LweI GCATC 1 cut(s) 322
MaeII ACGT 2 cut(s) 196, 810
MaeIII GTNAC 5 cut(s) 58, 73, 115, 197, 678
MalI GATC 1 cut(s) 723
MboI GATC 1 cut(s) 721
MboII GAAGA 2 cut(s) 195, 368
MflI RGATCY 1 cut(s) 721
MhlI GDGCHC 1 cut(s) 420
MluCI AATT 8 cut(s) 33, 184, 296, 303, 388, 432, 779, 818
MlyI GAGTC 2 cut(s) 543, 686
MmeI TCCRAC 1 cut(s) 697
MnlI CCTC 7 cut(s) 73, 161, 471, 547, 703, 748, 788
MseI TTAA 6 cut(s) 168, 192, 392, 593, 774, 883
MslI CAYNNNNRTG 1 cut(s) 859
MspCI CTTAAG 2 cut(s) 167, 773
Mva1269I GAATGC 1 cut(s) 572
MvnI CGCG 2 cut(s) 503, 505
MwoI GCNNNNNNNGC 3 cut(s) 494, 500, 638
NdeII GATC 1 cut(s) 721
NlaIII CATG 5 cut(s) 235, 410, 631, 646, 666
NmuCI GTSAC 3 cut(s) 73, 197, 678
PceI AGGCCT 1 cut(s) 364
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 1 cut(s) 587
Pfl23II CGTACG 1 cut(s) 506
PkrI GCNGC 2 cut(s) 105, 496
PleI GAGTC 2 cut(s) 543, 685
PpsI GAGTC 2 cut(s) 543, 685
Psp124BI GAGCTC 1 cut(s) 420
PspEI GGTNACC 1 cut(s) 58
PspFI CCCAGC 2 cut(s) 20, 865
PspLI CGTACG 1 cut(s) 506
PspPI GGNCC 1 cut(s) 580
PstI CTGCAG 1 cut(s) 70
PsuI RGATCY 1 cut(s) 721
RsaI GTAC 3 cut(s) 149, 508, 523
RsaNI GTAC 3 cut(s) 148, 507, 522
RseI CAYNNNNRTG 1 cut(s) 859
SacI GAGCTC 1 cut(s) 420
SaqAI TTAA 6 cut(s) 168, 192, 392, 593, 774, 883
SatI GCNGC 2 cut(s) 104, 495
Sau3AI GATC 1 cut(s) 721
Sau96I GGNCC 1 cut(s) 580
SchI GAGTC 2 cut(s) 543, 686
SduI GDGCHC 1 cut(s) 420
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 2 cut(s) 66, 669
SmiI ATTTAAAT 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 859
SmlI CTYRAG 2 cut(s) 167, 773
SmoI CTYRAG 2 cut(s) 167, 773
Sse9I AATT 8 cut(s) 33, 184, 296, 303, 388, 432, 779, 818
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 1 cut(s) 632
SstI GAGCTC 1 cut(s) 420
StuI AGGCCT 1 cut(s) 364
StyI CCWWGG 1 cut(s) 425
SwaI ATTTAAAT 1 cut(s) 393
TaaI ACNGT 4 cut(s) 329, 529, 755, 766
TaiI ACGT 2 cut(s) 199, 813
TaqI TCGA 1 cut(s) 686
TasI AATT 8 cut(s) 33, 184, 296, 303, 388, 432, 779, 818
TatI WGTACW 1 cut(s) 521
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 6 cut(s) 168, 192, 392, 593, 774, 883
Tru9I TTAA 6 cut(s) 168, 192, 392, 593, 774, 883
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 3 cut(s) 73, 197, 678
TseI GCWGC 2 cut(s) 103, 494
Tsp45I GTSAC 3 cut(s) 73, 197, 678
TspDTI ATGAA 3 cut(s) 105, 248, 296
TspRI CASTG 1 cut(s) 334
Vha464I CTTAAG 2 cut(s) 167, 773
XapI RAATTY 2 cut(s) 303, 779
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.