Rorug06G0273400

Vacuolar membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002838
Physical Location & Seq
Reverse (-)
42063143 .. 42065345
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0273400.1

Sequence Viewer

Length: 1278 bp
ATGGAAGCACCATTAGAAAGGCTGCCTCGCAGCAACACAGACATTACCACCAATAATGGTGGGAATGTTGTCTATTCCTTAGAAGAAGGGAAGGGAAGCCCAAGAAATGGTACTCACATGGTCTCTGATAAAAGAGCAGGTACTGAATTGCTATATGGAGCTACATCCAATAGTGGACATGGAATAGTGACTGTTGGAGTAGAGAGCGTACATTTTCAAAAGAGTGTTGAGACCAAGCACCAAGACGAGGATACTAGGAAGCTGAAGGATCTTGGCTCTTCATCCTCCGAGCAGACGGAGCAGTCCCCTGAAGAAAATGGAGTGGCTAAGCATCGAAAAATTAAGTTGAGAGATCTTGAAGGGGTGTTTGAGAAGACCGCTGCGCATGAATTCTACTGTCCTAATTGCAATGCTTGCATCACCGAGGTTATAGTCCGCGATAGAGAATCAAAACCTCCCACTCCACCACTTATTGAGGCAGATGAACCAAAACCTCCTACTCCACCACTTATTGAGGCAGATGAACCCGTCAGATGCACTTCGTGCTTTGGTCTCCTATTTCGTGCAGGCACTCGGATCTTTCGTCGACCAGAGCAAGAGAGCACAACAGTTATTACTGTGATCACACCGGAACAAACGCCGGCAGGGTCTGAATTACCACGAGATGTTAATAAGATGTTAGAAATCCTCAAAAGCATCGTTTACGGTGGTTTAAATGAAGCAATTGCAAGCTTAAGCATTGTGGCATCTGCAGCTAGTACTGATACTGCCACATTGAACATTTTCGTTTTGGCATTGGCAAACTTGCTGGGTGGACTTTTCGTCATCGGACACAATCTTTGGGACCTGAAAGCTGATCAAGTAGACAGTAAATACAAAGAAGTACTAGGGAACAAAGATAATTTCTTTTTTCATGCCCCGGTTGCCATACTCTCGTTCCTCATTTTCGGGTTAGTACCTCCTTTGGTTTATGGCTTCTCGTTCTCTCAGAGCGACGACAGCGACCTAAAGATTGCAGCAGTTGCAGCATCTTCTATTTTATGCATCATACTTCTCTCAATTGGAAAGGCTTACATCCAGACGCCACCAAAGTACATCAAAACTGTGCTGTACTATATCGTCATAGGGGTTGGGGCCGCCGGTGTTTCTTACTTAGTAGGTGACTTAATTGACAAACTTGTAGAGAAGCTTGGCTGGTTCAACTCAACTGTAGCTGGCTCTCTGCCTCTTCCTCATCAGACTATGAGTACAGTGAAGCCTGCATGGGAATCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

425

Amino Acids

46.19

Weight (kDa)

5.49

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 305 - 387 3.7e-08 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 301
Acc16I TGCGCA 1 cut(s) 384
Acc36I ACCTGC 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 107
AccI GTMKAC 2 cut(s) 586, 864
AccII CGCG 1 cut(s) 438
AciI CCGC 3 cut(s) 378, 436, 1137
AclWI GGATC 2 cut(s) 276, 584
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 2 cut(s) 284, 330
AcyI GRCGYC 1 cut(s) 1082
AdeI CACNNNGTG 1 cut(s) 543
AfaI GTAC 9 cut(s) 112, 142, 210, 760, 885, 957, 1094, 1112, 1249
AfiI CCNNNNNNNGG 2 cut(s) 107, 247
AflII CTTAAG 1 cut(s) 733
AgsI TTSAA 4 cut(s) 218, 359, 778, 1201
AhdI GACNNNNNGTC 1 cut(s) 821
AjnI CCWGG 1 cut(s) 1271
AluBI AGCT 7 cut(s) 161, 262, 732, 755, 854, 1189, 1214
AluI AGCT 7 cut(s) 161, 262, 732, 755, 854, 1189, 1214
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 3 cut(s) 127, 224, 557
AlwI GGATC 2 cut(s) 276, 584
AlwNI CAGNNNCTG 2 cut(s) 143, 650
AoxI GGCC 1 cut(s) 1134
ApeKI GCWGC 6 cut(s) 22, 30, 380, 752, 1016, 1025
ApoI RAATTY 1 cut(s) 389
ArsI GACNNNNNNTTYG 2 cut(s) 822, 854
Asp700I GAANNNNTTC 1 cut(s) 782
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 844, 1134
AsuC2I CCSGG 1 cut(s) 920
AsuHPI GGTGA 2 cut(s) 412, 1172
AvaII GGWCC 1 cut(s) 844
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 605
BbvI GCAGC 6 cut(s) 9, 42, 367, 764, 1028, 1037
BciT130I CCWGG 1 cut(s) 1273
BciVI GTATCC 1 cut(s) 244
BclI TGATCA 2 cut(s) 621, 856
BcnI CCSGG 1 cut(s) 920
BcoDI GTCTC 3 cut(s) 127, 224, 557
BfaI CTAG 3 cut(s) 255, 756, 887
BfmI CTRYAG 2 cut(s) 750, 1209
BfrI CTTAAG 1 cut(s) 733
BfuAI ACCTGC 1 cut(s) 128
BfuI GTATCC 1 cut(s) 244
BglII AGATCT 1 cut(s) 352
BisI GCNGC 7 cut(s) 23, 31, 381, 753, 1017, 1026, 1137
BlpI GCTNAGC 1 cut(s) 327
BlsI GCNGC 7 cut(s) 24, 32, 382, 754, 1018, 1027, 1138
BmcAI AGTACT 2 cut(s) 760, 885
Bme1390I CCNGG 2 cut(s) 920, 1273
Bme18I GGWCC 1 cut(s) 844
BmeRI GACNNNNNGTC 1 cut(s) 821
BmgT120I GGNCC 2 cut(s) 844, 1134
BmiI GGNNCC 2 cut(s) 845, 1135
BmrFI CCNGG 2 cut(s) 920, 1273
BmsI GCATC 7 cut(s) 340, 426, 524, 705, 755, 1037, 1053
BpiI GAAGAC 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 920
BsaHI GRCGYC 1 cut(s) 1082
BsaI GGTCTC 3 cut(s) 127, 224, 557
BsaJI CCNNGG 2 cut(s) 423, 918
BsaWI WCCGGW 1 cut(s) 628
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 247
Bse118I RCCGGY 2 cut(s) 640, 1139
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 1273
BseDI CCNNGG 2 cut(s) 423, 918
BseGI GGATG 3 cut(s) 164, 281, 1074
BseLI CCNNNNNNNGG 2 cut(s) 107, 247
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 1001
BseXI GCAGC 6 cut(s) 9, 42, 367, 764, 1028, 1037
BseYI CCCAGC 1 cut(s) 808
BsgI GTGCAG 1 cut(s) 585
Bsh1236I CGCG 1 cut(s) 438
BshFI GGCC 1 cut(s) 1136
BsiHKAI GWGCWC 1 cut(s) 605
BsiSI CCGG 4 cut(s) 629, 641, 920, 1140
BslFI GGGAC 2 cut(s) 289, 857
BslI CCNNNNNNNGG 2 cut(s) 107, 247
BsmAI GTCTC 3 cut(s) 127, 224, 557
BsmFI GGGAC 2 cut(s) 289, 857
BsnI GGCC 1 cut(s) 1136
Bso31I GGTCTC 3 cut(s) 127, 224, 557
Bsp1286I GDGCHC 1 cut(s) 605
Bsp143I GATC 5 cut(s) 268, 352, 576, 621, 856
Bsp1720I GCTNAGC 1 cut(s) 327
BspACI CCGC 3 cut(s) 378, 436, 1137
BspANI GGCC 1 cut(s) 1136
BspCNI CTCAG 1 cut(s) 1000
BspFNI CGCG 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 845, 1135
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 2 cut(s) 276, 584
BspQI GCTCTTC 1 cut(s) 283
BspTI CTTAAG 1 cut(s) 733
BspTNI GGTCTC 3 cut(s) 127, 224, 557
BsrDI GCAATG 1 cut(s) 415
BsrFI RCCGGY 2 cut(s) 640, 1139
BssAI RCCGGY 2 cut(s) 640, 1139
BssECI CCNNGG 2 cut(s) 423, 918
BssMI GATC 5 cut(s) 268, 352, 576, 621, 856
BssNI GRCGYC 1 cut(s) 1082
BssSI CACGAG 1 cut(s) 660
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 1 cut(s) 1273
Bst4CI ACNGT 9 cut(s) 193, 398, 610, 619, 707, 869, 1105, 1210, 1252
Bst6I CTCTTC 2 cut(s) 283, 1233
BstACI GRCGYC 1 cut(s) 1082
BstAFI CTTAAG 1 cut(s) 733
BstAPI GCANNNNNTGC 3 cut(s) 414, 543, 1022
BstC8I GCNNGC 6 cut(s) 415, 568, 642, 730, 1216, 1260
BstDEI CTNAG 4 cut(s) 79, 327, 987, 1153
BstF5I GGATG 3 cut(s) 164, 281, 1074
BstFNI CGCG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 5 cut(s) 271, 355, 579, 624, 859
BstMAI GTCTC 3 cut(s) 127, 224, 557
BstMBI GATC 5 cut(s) 268, 352, 576, 621, 856
BstMWI GCNNNNNNNGC 8 cut(s) 298, 414, 543, 752, 923, 999, 1022, 1025
BstNI CCWGG 1 cut(s) 1273
BstSCI CCNGG 2 cut(s) 918, 1271
BstSFI CTRYAG 2 cut(s) 750, 1209
BstUI CGCG 1 cut(s) 438
BstV1I GCAGC 6 cut(s) 9, 42, 367, 764, 1028, 1037
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 3 cut(s) 268, 352, 576
BstYI RGATCY 3 cut(s) 268, 352, 576
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 1 cut(s) 1136
BtsCI GGATG 3 cut(s) 164, 281, 1074
BtsIMutI CAGTG 1 cut(s) 1257
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 6 cut(s) 415, 568, 642, 730, 1216, 1260
CaiI CAGNNNCTG 2 cut(s) 143, 650
CfoI GCGC 1 cut(s) 385
Cfr10I RCCGGY 2 cut(s) 640, 1139
Cfr13I GGNCC 2 cut(s) 844, 1134
CseI GACGC 1 cut(s) 1090
Csp6I GTAC 9 cut(s) 111, 141, 209, 759, 884, 956, 1093, 1111, 1248
CspCI CAANNNNNGTGG 2 cut(s) 40, 75
CviAII CATG 5 cut(s) 118, 179, 386, 914, 1263
CviQI GTAC 9 cut(s) 111, 141, 209, 759, 884, 956, 1093, 1111, 1248
DdeI CTNAG 4 cut(s) 79, 327, 987, 1153
DpnI GATC 5 cut(s) 270, 354, 578, 623, 858
DpnII GATC 5 cut(s) 268, 352, 576, 621, 856
DraI TTTAAA 1 cut(s) 714
DraIII CACNNNGTG 1 cut(s) 543
DrdI GACNNNNNNGTC 1 cut(s) 301
DriI GACNNNNNGTC 1 cut(s) 821
DseDI GACNNNNNNGTC 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 283, 1233
Eam1105I GACNNNNNGTC 1 cut(s) 821
EarI CTCTTC 2 cut(s) 283, 1233
Eco31I GGTCTC 3 cut(s) 127, 224, 557
Eco47I GGWCC 1 cut(s) 844
Eco57I CTGAAG 2 cut(s) 284, 330
EcoO109I RGGNCCY 1 cut(s) 844
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 1271
EcoT22I ATGCAT 1 cut(s) 1046
FaeI CATG 5 cut(s) 121, 182, 389, 917, 1266
FaqI GGGAC 2 cut(s) 289, 857
FatI CATG 5 cut(s) 117, 178, 385, 913, 1262
FbaI TGATCA 2 cut(s) 621, 856
FblI GTMKAC 2 cut(s) 586, 864
Fnu4HI GCNGC 7 cut(s) 23, 31, 381, 753, 1017, 1026, 1137
FokI GGATG 3 cut(s) 151, 268, 1061
Fsp4HI GCNGC 7 cut(s) 23, 31, 381, 753, 1017, 1026, 1137
FspBI CTAG 3 cut(s) 255, 756, 887
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 1 cut(s) 384
GluI GCNGC 7 cut(s) 23, 31, 381, 753, 1017, 1026, 1137
GsaI CCCAGC 1 cut(s) 812
HaeIII GGCC 1 cut(s) 1136
HapII CCGG 4 cut(s) 629, 641, 920, 1140
HgaI GACGC 1 cut(s) 1090
HhaI GCGC 1 cut(s) 385
Hin1I GRCGYC 1 cut(s) 1082
Hin1II CATG 5 cut(s) 121, 182, 389, 917, 1266
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HincII GTYRAC 1 cut(s) 587
HindII GTYRAC 1 cut(s) 587
HindIII AAGCTT 2 cut(s) 730, 1187
HinfI GANTC 2 cut(s) 446, 1268
HpaII CCGG 4 cut(s) 629, 641, 920, 1140
HphI GGTGA 2 cut(s) 412, 1172
Hpy166II GTNNAC 5 cut(s) 176, 587, 703, 815, 865
Hpy188I TCNGA 8 cut(s) 127, 289, 533, 576, 652, 830, 990, 1239
Hpy188III TCNNGA 2 cut(s) 356, 1078
Hpy8I GTNNAC 5 cut(s) 176, 587, 703, 815, 865
Hpy99I CGWCG 2 cut(s) 588, 998
HpyAV CCTTC 4 cut(s) 80, 85, 259, 353
HpyCH4III ACNGT 9 cut(s) 193, 398, 610, 619, 707, 869, 1105, 1210, 1252
HpyF10VI GCNNNNNNNGC 8 cut(s) 298, 414, 543, 752, 923, 999, 1022, 1025
HpyF3I CTNAG 4 cut(s) 79, 327, 987, 1153
Hsp92I GRCGYC 1 cut(s) 1082
Hsp92II CATG 5 cut(s) 121, 182, 389, 917, 1266
HspAI GCGC 1 cut(s) 383
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
Ksp22I TGATCA 2 cut(s) 621, 856
Kzo9I GATC 5 cut(s) 268, 352, 576, 621, 856
LguI GCTCTTC 1 cut(s) 283
LmnI GCTCC 2 cut(s) 158, 298
Lsp1109I GCAGC 6 cut(s) 9, 42, 367, 764, 1028, 1037
LweI GCATC 7 cut(s) 340, 426, 524, 705, 755, 1037, 1053
MaeI CTAG 3 cut(s) 255, 756, 887
MaeIII GTNAC 2 cut(s) 187, 1160
MalI GATC 5 cut(s) 270, 354, 578, 623, 858
MboI GATC 5 cut(s) 268, 352, 576, 621, 856
MboII GAAGA 6 cut(s) 95, 270, 323, 385, 1023, 1220
MfeI CAATTG 2 cut(s) 723, 1059
MflI RGATCY 3 cut(s) 268, 352, 576
MhlI GDGCHC 1 cut(s) 605
MluCI AATT 9 cut(s) 146, 339, 389, 403, 653, 723, 901, 1059, 1167
MmeI TCCRAC 1 cut(s) 175
Mph1103I ATGCAT 1 cut(s) 1046
MroNI GCCGGC 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 782
MseI TTAA 5 cut(s) 342, 669, 713, 734, 1166
MspA1I CMGCKG 1 cut(s) 380
MspCI CTTAAG 1 cut(s) 733
MspI CCGG 4 cut(s) 629, 641, 920, 1140
MspR9I CCNGG 2 cut(s) 920, 1273
MunI CAATTG 2 cut(s) 723, 1059
MvaI CCWGG 1 cut(s) 1273
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 8 cut(s) 298, 414, 543, 752, 923, 999, 1022, 1025
NaeI GCCGGC 1 cut(s) 642
NciI CCSGG 1 cut(s) 920
NdeII GATC 5 cut(s) 268, 352, 576, 621, 856
NgoMIV GCCGGC 1 cut(s) 640
NlaIII CATG 5 cut(s) 121, 182, 389, 917, 1266
NlaIV GGNNCC 2 cut(s) 845, 1135
NmuCI GTSAC 2 cut(s) 187, 1160
NsbI TGCGCA 1 cut(s) 384
NsiI ATGCAT 1 cut(s) 1046
PciSI GCTCTTC 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 580
PdiI GCCGGC 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 782
PfeI GAWTC 2 cut(s) 446, 1268
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 7 cut(s) 24, 32, 382, 754, 1018, 1027, 1138
PpuMI RGGWCCY 1 cut(s) 844
Psp5II RGGWCCY 1 cut(s) 844
Psp6I CCWGG 1 cut(s) 1271
PspFI CCCAGC 1 cut(s) 808
PspGI CCWGG 1 cut(s) 1271
PspN4I GGNNCC 2 cut(s) 845, 1135
PspPI GGNCC 2 cut(s) 844, 1134
PspPPI RGGWCCY 1 cut(s) 844
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 2 cut(s) 143, 650
PsuI RGATCY 3 cut(s) 268, 352, 576
RsaI GTAC 9 cut(s) 112, 142, 210, 760, 885, 957, 1094, 1112, 1249
RsaNI GTAC 9 cut(s) 111, 141, 209, 759, 884, 956, 1093, 1111, 1248
SalI GTCGAC 1 cut(s) 585
SapI GCTCTTC 1 cut(s) 283
SaqAI TTAA 5 cut(s) 342, 669, 713, 734, 1166
SatI GCNGC 7 cut(s) 23, 31, 381, 753, 1017, 1026, 1137
Sau3AI GATC 5 cut(s) 268, 352, 576, 621, 856
Sau96I GGNCC 2 cut(s) 844, 1134
ScaI AGTACT 2 cut(s) 760, 885
ScrFI CCNGG 2 cut(s) 920, 1273
SduI GDGCHC 1 cut(s) 605
SfaNI GCATC 7 cut(s) 340, 426, 524, 705, 755, 1037, 1053
SfcI CTRYAG 2 cut(s) 750, 1209
SgrAI CRCCGGYG 1 cut(s) 1139
SinI GGWCC 1 cut(s) 844
SmlI CTYRAG 1 cut(s) 733
SmoI CTYRAG 1 cut(s) 733
Sse9I AATT 9 cut(s) 146, 339, 389, 403, 653, 723, 901, 1059, 1167
SsiI CCGC 3 cut(s) 378, 436, 1137
SspMI CTAG 3 cut(s) 255, 756, 887
StyD4I CCNGG 2 cut(s) 918, 1271
TaaI ACNGT 9 cut(s) 193, 398, 610, 619, 707, 869, 1105, 1210, 1252
TaqI TCGA 2 cut(s) 334, 586
TasI AATT 9 cut(s) 146, 339, 389, 403, 653, 723, 901, 1059, 1167
TatI WGTACW 5 cut(s) 758, 883, 1092, 1110, 1247
TauI GCSGC 1 cut(s) 1139
TfiI GAWTC 2 cut(s) 446, 1268
Tru1I TTAA 5 cut(s) 342, 669, 713, 734, 1166
Tru9I TTAA 5 cut(s) 342, 669, 713, 734, 1166
TscAI CASTG 1 cut(s) 1257
TseFI GTSAC 2 cut(s) 187, 1160
TseI GCWGC 6 cut(s) 22, 30, 380, 752, 1016, 1025
Tsp45I GTSAC 2 cut(s) 187, 1160
TspDTI ATGAA 6 cut(s) 270, 402, 498, 537, 732, 902
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 1 cut(s) 1257
Van91I CCANNNNNTGG 1 cut(s) 107
Vha464I CTTAAG 1 cut(s) 733
VpaK11BI GGWCC 1 cut(s) 844
XapI RAATTY 1 cut(s) 389
XmiI GTMKAC 2 cut(s) 586, 864
XmnI GAANNNNTTC 1 cut(s) 782
XspI CTAG 3 cut(s) 255, 756, 887
ZrmI AGTACT 2 cut(s) 760, 885
Zsp2I ATGCAT 1 cut(s) 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.