MD15G1043000.v1.1

Solute carrier family 35 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002838
Physical Location & Seq
Reverse (-)
2989235 .. 2992442
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1043000.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGGTTGGAAGTACAACGCCGGTTTGGGGTTGATTGGCACGGTTGTGTTCATCTGGGTCGCTTCTGCAGAGGTTACTCAGAGAATTTTCAGCAAGTACAAACAGCCATTTTCGCTCACTTATCTCGGTGTGTCTCTAATGGTGGTTTATCTGCCCATATCAGCTTTGAAAGATTGGCTCTGCAACTTATTGGACCGAAATTTAGTTAAGAACTTTTGCAATGTCAGTGCTCTGTGCACCTCAGTCGGGCTTGATATTCCTCTTAGAATAAATGAAATGCCCCACGTTCCAGAAACAGATGTTAGAAGCTGCTTAATAACTGACAAAGATCTTAGTAACACGGAAGAAGGGCGACCTCTAATGGATAAGAGTGAAGAAGATGAACCTCGCATGCTTGAACACAGTCGTCAACTTAGTCCATGCGAAATTGCAAAATTTGGCTTGATTCTTACTCCGTTGTGGTTTATCACAGAGTATCTGTCGAACTCTGCACTGGCAAATACCAGTGTTGCAAGTACAACTGTCCTAACTTCTACATCGGGGCTCTTTACACTTTTCTTTGGAGCTCTTCTGCATCAGGATTCCATAACTATTGCAAAGATGGTTGCTGTTTGTGTCACCATGGCTGGTGTTGCCATGACCACTGTTGGAAAAACTTGGGCACCTGATGAACTGCTGAGCATATCTGAGATTAGAAAGCACTCTATAATGGGAGATATTTTTGGTCTTCTCTCAGCTGCGTCATATGGATTGTTTACAGTGTTACTCAAGAAATTTGCTGGATCAGAGGAGAAAGGAGATAAGGCTGATGTGCAGAAGCTTTTCGGATACATTGGTCTCTTCACTGTGTTTGGTCTGTGGTGGCTCGTGTGGCCGCTCAATGCCATCGGGATTGAACCTTCATTTGAGTTTCCACATTCAAAATGTATTGCCGAAGCTGTGCTGATAAATGGCTTTGTTGGGAGTGTTCTTTCGGATTATTTTTGGGCTCTTTCCGTGGTTTGGACATCTCCATTAGTTGCAACGCTGGGCATGTCCTTGACAATTCCACTTGCAATGGTAGCCGACATGGTGATTCACGGCCGCCATTTTTCTGCACTCTACATCTTTGGTTGCGTTCAGGTTTTTGCAGGATTTGTGATGGCAAACGTTTCGGACAGGTTCTCGAGCAAGCAAGAGTTGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

396

Amino Acids

43.49

Weight (kDa)

5.55

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 155 - 215 1e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 661
AccBSI CCGCTC 1 cut(s) 877
AciI CCGC 2 cut(s) 875, 1084
AclI AACGTT 1 cut(s) 1149
AclWI GGATC 1 cut(s) 790
AcoI YGGCCR 2 cut(s) 872, 1081
AcsI RAATTY 4 cut(s) 84, 199, 434, 773
AfaI GTAC 3 cut(s) 14, 98, 517
AfiI CCNNNNNNNGG 3 cut(s) 27, 246, 1002
AgsI TTSAA 4 cut(s) 169, 398, 896, 921
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 6 cut(s) 164, 309, 566, 737, 820, 938
AluI AGCT 6 cut(s) 164, 309, 566, 737, 820, 938
Alw21I GWGCWC 3 cut(s) 232, 239, 568
Alw26I GTCTC 2 cut(s) 138, 842
Alw44I GTGCAC 1 cut(s) 235
AlwI GGATC 1 cut(s) 790
Ama87I CYCGRG 1 cut(s) 1165
AoxI GGCC 2 cut(s) 872, 1081
ApaLI GTGCAC 1 cut(s) 235
ApeKI GCWGC 2 cut(s) 309, 737
ApoI RAATTY 4 cut(s) 84, 199, 434, 773
Asp700I GAANNNNTTC 1 cut(s) 821
AspS9I GGNCC 1 cut(s) 193
AsuHPI GGTGA 2 cut(s) 610, 1084
AvaI CYCGRG 1 cut(s) 1165
AvaII GGWCC 1 cut(s) 193
BaeGI GKGCMC 2 cut(s) 239, 664
BaeI ACNNNNGTAYC 2 cut(s) 820, 853
BanI GGYRCC 1 cut(s) 661
BanII GRGCYC 3 cut(s) 546, 568, 991
BauI CACGAG 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 719
Bbv12I GWGCWC 3 cut(s) 232, 239, 568
BbvI GCAGC 2 cut(s) 296, 724
BccI CCATC 3 cut(s) 595, 893, 1135
BceAI ACGGC 1 cut(s) 1096
BcgI CGANNNNNNTGC 4 cut(s) 226, 260, 1134, 1168
BciVI GTATCC 1 cut(s) 821
BcoDI GTCTC 2 cut(s) 138, 842
BfmI CTRYAG 1 cut(s) 66
BfuI GTATCC 1 cut(s) 821
BglII AGATCT 1 cut(s) 328
BisI GCNGC 4 cut(s) 310, 738, 875, 1084
BlpI GCTNAGC 1 cut(s) 677
BlsI GCNGC 4 cut(s) 311, 739, 876, 1085
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 1165
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 663
BmsI GCATC 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 719
Bpu1102I GCTNAGC 1 cut(s) 677
BpuEI CTTGAG 1 cut(s) 752
BsaI GGTCTC 1 cut(s) 842
BsaJI CCNNGG 2 cut(s) 621, 996
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 246, 1002
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 2 cut(s) 498, 504
Bse3DI GCAATG 2 cut(s) 226, 1062
BseDI CCNNGG 2 cut(s) 621, 996
BseLI CCNNNNNNNGG 3 cut(s) 27, 246, 1002
BseMI GCAATG 2 cut(s) 226, 1062
BseMII CTCAG 5 cut(s) 92, 255, 668, 678, 747
BseNI ACTGG 2 cut(s) 498, 504
BseRI GAGGAG 1 cut(s) 803
BseSI GKGCMC 2 cut(s) 239, 664
BseX3I CGGCCG 1 cut(s) 1081
BseXI GCAGC 2 cut(s) 296, 724
BseYI CCCAGC 1 cut(s) 1027
BsgI GTGCAG 3 cut(s) 474, 833, 1080
Bsh1285I CGRYCG 1 cut(s) 1084
BshFI GGCC 2 cut(s) 874, 1083
BshNI GGYRCC 1 cut(s) 661
BsiEI CGRYCG 1 cut(s) 1084
BsiHKAI GWGCWC 3 cut(s) 232, 239, 568
BsiHKCI CYCGRG 1 cut(s) 1165
BsiSI CCGG 1 cut(s) 21
BslI CCNNNNNNNGG 3 cut(s) 27, 246, 1002
BsmAI GTCTC 2 cut(s) 138, 842
BsnI GGCC 2 cut(s) 874, 1083
Bso31I GGTCTC 1 cut(s) 842
BsoBI CYCGRG 1 cut(s) 1165
Bsp1286I GDGCHC 6 cut(s) 232, 239, 546, 568, 664, 991
Bsp143I GATC 2 cut(s) 328, 782
Bsp1720I GCTNAGC 1 cut(s) 677
Bsp19I CCATGG 1 cut(s) 621
BspACI CCGC 2 cut(s) 875, 1084
BspANI GGCC 2 cut(s) 874, 1083
BspCNI CTCAG 5 cut(s) 91, 254, 669, 679, 746
BspLI GGNNCC 1 cut(s) 663
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 1 cut(s) 790
BspQI GCTCTTC 1 cut(s) 573
BspT107I GGYRCC 1 cut(s) 661
BspTNI GGTCTC 1 cut(s) 842
BsrBI CCGCTC 1 cut(s) 877
BsrDI GCAATG 2 cut(s) 226, 1062
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 2 cut(s) 498, 504
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 621, 996
BssMI GATC 2 cut(s) 328, 782
BssSI CACGAG 1 cut(s) 866
BssT1I CCWWGG 1 cut(s) 621
Bst2BI CACGAG 1 cut(s) 866
Bst4CI ACNGT 6 cut(s) 43, 404, 523, 646, 760, 847
Bst6I CTCTTC 2 cut(s) 573, 845
BstAPI GCANNNNNTGC 1 cut(s) 1180
BstC8I GCNNGC 2 cut(s) 392, 1172
BstDEI CTNAG 8 cut(s) 78, 241, 263, 332, 413, 677, 687, 733
BstDSI CCRYGG 2 cut(s) 621, 996
BstKTI GATC 2 cut(s) 331, 785
BstMAI GTCTC 2 cut(s) 138, 842
BstMBI GATC 2 cut(s) 328, 782
BstMCI CGRYCG 1 cut(s) 1084
BstMWI GCNNNNNNNGC 5 cut(s) 112, 632, 871, 1061, 1180
BstNSI RCATGY 2 cut(s) 394, 1036
BstSFI CTRYAG 1 cut(s) 66
BstSLI GKGCMC 2 cut(s) 239, 664
BstV1I GCAGC 2 cut(s) 296, 724
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BstZI CGGCCG 1 cut(s) 1081
BsuI GTATCC 1 cut(s) 821
BsuRI GGCC 2 cut(s) 874, 1083
BtgI CCRYGG 2 cut(s) 621, 996
BtsIMutI CAGTG 6 cut(s) 232, 491, 511, 642, 765, 843
Cac8I GCNNGC 2 cut(s) 392, 1172
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 193
CseI GACGC 1 cut(s) 729
Csp6I GTAC 3 cut(s) 13, 97, 516
CviAII CATG 6 cut(s) 391, 420, 622, 637, 1033, 1069
CviQI GTAC 3 cut(s) 13, 97, 516
DdeI CTNAG 8 cut(s) 78, 241, 263, 332, 413, 677, 687, 733
DpnI GATC 2 cut(s) 330, 784
DpnII GATC 2 cut(s) 328, 782
EaeI YGGCCR 2 cut(s) 872, 1081
EagI CGGCCG 1 cut(s) 1081
Eam1104I CTCTTC 2 cut(s) 573, 845
EarI CTCTTC 2 cut(s) 573, 845
Ecl136II GAGCTC 1 cut(s) 566
EclXI CGGCCG 1 cut(s) 1081
Eco130I CCWWGG 1 cut(s) 621
Eco24I GRGCYC 3 cut(s) 546, 568, 991
Eco31I GGTCTC 1 cut(s) 842
Eco47I GGWCC 1 cut(s) 193
Eco52I CGGCCG 1 cut(s) 1081
Eco53kI GAGCTC 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 1165
EcoICRI GAGCTC 1 cut(s) 566
EcoT14I CCWWGG 1 cut(s) 621
EcoT38I GRGCYC 3 cut(s) 546, 568, 991
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 6 cut(s) 394, 423, 625, 640, 1036, 1072
FatI CATG 6 cut(s) 390, 419, 621, 636, 1032, 1068
FauNDI CATATG 1 cut(s) 745
Fnu4HI GCNGC 4 cut(s) 310, 738, 875, 1084
FriOI GRGCYC 3 cut(s) 546, 568, 991
Fsp4HI GCNGC 4 cut(s) 310, 738, 875, 1084
GluI GCNGC 4 cut(s) 310, 738, 875, 1084
GsaI CCCAGC 1 cut(s) 1031
HaeIII GGCC 2 cut(s) 874, 1083
HapII CCGG 1 cut(s) 21
HgaI GACGC 1 cut(s) 729
Hin1II CATG 6 cut(s) 394, 423, 625, 640, 1036, 1072
HincII GTYRAC 1 cut(s) 410
HindII GTYRAC 1 cut(s) 410
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 3 cut(s) 445, 581, 1075
HpaII CCGG 1 cut(s) 21
HphI GGTGA 2 cut(s) 610, 1084
Hpy166II GTNNAC 3 cut(s) 237, 410, 756
Hpy188I TCNGA 6 cut(s) 81, 688, 787, 827, 976, 1156
Hpy188III TCNNGA 5 cut(s) 290, 578, 769, 889, 1165
Hpy8I GTNNAC 3 cut(s) 237, 410, 756
HpyAV CCTTC 2 cut(s) 341, 909
HpyCH4III ACNGT 6 cut(s) 43, 404, 523, 646, 760, 847
HpyCH4IV ACGT 2 cut(s) 285, 1149
HpyF10VI GCNNNNNNNGC 5 cut(s) 112, 632, 871, 1061, 1180
HpyF3I CTNAG 8 cut(s) 78, 241, 263, 332, 413, 677, 687, 733
HpySE526I ACGT 2 cut(s) 285, 1149
Hsp92II CATG 6 cut(s) 394, 423, 625, 640, 1036, 1072
Kzo9I GATC 2 cut(s) 328, 782
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 1 cut(s) 563
Lsp1109I GCAGC 2 cut(s) 296, 724
LweI GCATC 1 cut(s) 583
MaeII ACGT 2 cut(s) 285, 1149
MaeIII GTNAC 4 cut(s) 73, 335, 616, 762
MalI GATC 2 cut(s) 330, 784
MbiI CCGCTC 1 cut(s) 877
MboI GATC 2 cut(s) 328, 782
MboII GAAGA 6 cut(s) 356, 386, 389, 560, 719, 832
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 6 cut(s) 232, 239, 546, 568, 664, 991
MluCI AATT 6 cut(s) 84, 199, 426, 434, 773, 1044
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 6 cut(s) 64, 250, 270, 366, 396, 781
MroXI GAANNNNTTC 1 cut(s) 821
MseI TTAA 2 cut(s) 207, 314
MslI CAYNNNNRTG 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 1 cut(s) 21
MwoI GCNNNNNNNGC 5 cut(s) 112, 632, 871, 1061, 1180
NcoI CCATGG 1 cut(s) 621
NdeI CATATG 1 cut(s) 745
NdeII GATC 2 cut(s) 328, 782
NlaIII CATG 6 cut(s) 394, 423, 625, 640, 1036, 1072
NlaIV GGNNCC 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 616
NspI RCATGY 2 cut(s) 394, 1036
OliI CACNNNNGTG 1 cut(s) 44
PaeI GCATGC 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 1165
PciSI GCTCTTC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 3 cut(s) 445, 581, 1075
PkrI GCNGC 4 cut(s) 311, 739, 876, 1085
Psp124BI GAGCTC 1 cut(s) 568
Psp1406I AACGTT 1 cut(s) 1149
PspFI CCCAGC 1 cut(s) 1027
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 1 cut(s) 193
PstI CTGCAG 1 cut(s) 70
PsuI RGATCY 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 3 cut(s) 14, 98, 517
RsaNI GTAC 3 cut(s) 13, 97, 516
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 1 cut(s) 568
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 207, 314
SatI GCNGC 4 cut(s) 310, 738, 875, 1084
Sau3AI GATC 2 cut(s) 328, 782
Sau96I GGNCC 1 cut(s) 193
SduI GDGCHC 6 cut(s) 232, 239, 546, 568, 664, 991
SfaNI GCATC 1 cut(s) 583
SfcI CTRYAG 1 cut(s) 66
Sfr274I CTCGAG 1 cut(s) 1165
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 1165
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 767, 1165
SmoI CTYRAG 2 cut(s) 767, 1165
SphI GCATGC 1 cut(s) 394
Sse9I AATT 6 cut(s) 84, 199, 426, 434, 773, 1044
SsiI CCGC 2 cut(s) 875, 1084
SstI GAGCTC 1 cut(s) 568
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 6 cut(s) 43, 404, 523, 646, 760, 847
TaiI ACGT 2 cut(s) 288, 1152
TaqI TCGA 2 cut(s) 482, 1166
TaqII GACCGA 1 cut(s) 210
TasI AATT 6 cut(s) 84, 199, 426, 434, 773, 1044
TatI WGTACW 3 cut(s) 12, 96, 515
TauI GCSGC 2 cut(s) 877, 1086
TfiI GAWTC 3 cut(s) 445, 581, 1075
Tru1I TTAA 2 cut(s) 207, 314
Tru9I TTAA 2 cut(s) 207, 314
TscAI CASTG 6 cut(s) 232, 498, 511, 649, 765, 850
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 2 cut(s) 309, 737
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 5 cut(s) 40, 288, 396, 684, 891
TspGWI ACGGA 3 cut(s) 356, 444, 985
TspRI CASTG 6 cut(s) 232, 498, 511, 649, 765, 850
VneI GTGCAC 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 4 cut(s) 84, 199, 434, 773
XceI RCATGY 2 cut(s) 394, 1036
XhoI CTCGAG 1 cut(s) 1165
XmnI GAANNNNTTC 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.