FvH4_2g28780

Vacuolar membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002838
Physical Location & Seq
Reverse (-)
22469807 .. 22473789
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28780.t2

Sequence Viewer

Length: 1188 bp
ATGGGTGGTTGGAGGTACAGAGCTGGGTTGCTTCTGATTGGTGCTGTGGTTATCATTTGGGTTACCTCTGCGGAAGTCACGCAGGATATTTATGCAAAATATAAGCAGCCATTTGCAGTAACATATCTTGGAGCTTCTCTTATGGTAGTGTACCTCCCAATAGCTTTCCTTAAGGACTGGTTGTGTAATTTACTTAAACGTCGCTCTTCTAAAAGTGGCAAAAATGCAGAAAGCATGAATGAGTATTCTTCTGCGTTTGGTTCACCACTGAAACACAATGGAGTTCAGAAAGATTTTGAATTGGAAATTCATACCTTGTCCAGAAAAGACAGTGATGCAGACCTTTCACCTTGTGCAGAAGAAAGGCCTTTGGTTTCTAAACTCAAAGACAATATAAATGTGCTGAAGCATGATCGAGAACTGACACCAAGGGAAATTGCTACTTATGGATTTTACCTTGCCCCTCTTTGGTTTTTTACAGAGTATTTTTCAAATGCCGCTCTCGCACGTACGAGTGTTGCAAGTACAACAGTCTTATCCTCAACTTCAGGACTCTTTACACTCTTCATTGGTGCATTCTTAGGCCAAGATTCTTTGAATCTAGCAAAAGTGGTGGCCGTCTTTGTCAGCATGGCGGGTGTAGCCATGACAACATTGGGAAAAACGTGGGCTACAGATGAGTCGCAATCGAGTGCCTCCAATGGGAAACACTCTATCGCTGGAGATTTCCTTGGCCTTGCCTCAGCCATGTCATATGGTCTATTTACAGTTCTTCTTAAGAAATTTGGTGGTGAAGGAGGAGAGAAGGCTGACGTGCAAAAGTTGTTTGGATATATTGGACTGTTTACGCTTGTAGCATTATGGTGGCTGGTTTTACCATTGACAGCAATGGGCATTGAACCGAAGTTCATGATTCCTCACTCTGCTAGACTGGATGAACTTGTTATTGCAAATGGCTTTGTTGGAAGTGTACTTTCTGACTACTTTTGGGCACTATGTGTTGTGTGGACAACTCCACTGGTGGCCACTTTGGGCATGTCACTCACCATCCCCCTAGCTATGGTGGCTGACATGTTCATCCATGGTCGTCATTATTCAGCAGTTTACATCCTTGGCTCTGTACAGGTATTTGCAGGATTTGTAATAGCTAATCTTTCAGACTGGTTCTCAAAGAAGTTGGGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

396

Amino Acids

43.19

Weight (kDa)

8.25

Isoelectric Point (pI)

29.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 141 - 218 1.6e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 500
AciI CCGC 3 cut(s) 71, 498, 635
AcoI YGGCCR 2 cut(s) 615, 1023
AcsI RAATTY 2 cut(s) 306, 782
AcuI CTGAAG 2 cut(s) 425, 531
AdeI CACNNNGTG 2 cut(s) 353, 998
AfaI GTAC 6 cut(s) 17, 152, 511, 526, 972, 1122
AfiI CCNNNNNNNGG 3 cut(s) 468, 702, 1060
AflII CTTAAG 2 cut(s) 170, 776
AflIII ACRYGT 1 cut(s) 1071
AgsI TTSAA 4 cut(s) 299, 492, 598, 899
AjiI CACGTC 1 cut(s) 814
AluBI AGCT 5 cut(s) 23, 134, 164, 1058, 1148
AluI AGCT 5 cut(s) 23, 134, 164, 1058, 1148
AoxI GGCC 5 cut(s) 365, 583, 615, 733, 1023
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 2 cut(s) 306, 782
AsuHPI GGTGA 4 cut(s) 255, 339, 803, 1036
BaeGI GKGCMC 1 cut(s) 994
BalI TGGCCA 1 cut(s) 1025
BbvCI CCTCAGC 1 cut(s) 742
BbvI GCAGC 1 cut(s) 118
BccI CCATC 1 cut(s) 1055
BceAI ACGGC 1 cut(s) 602
BfaI CTAG 3 cut(s) 602, 927, 1055
BfmI CTRYAG 1 cut(s) 672
BfrI CTTAAG 2 cut(s) 170, 776
BisI GCNGC 2 cut(s) 107, 498
BlsI GCNGC 2 cut(s) 108, 499
BmgBI CACGTC 1 cut(s) 814
BmsI GCATC 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 741
Bpu10I CCTNAGC 1 cut(s) 742
BsaAI YACGTR 1 cut(s) 509
BsaJI CCNNGG 4 cut(s) 428, 730, 1081, 1111
Bsc4I CCNNNNNNNGG 3 cut(s) 468, 702, 1060
Bse1I ACTGG 4 cut(s) 182, 936, 1023, 1166
Bse3DI GCAATG 1 cut(s) 894
BseDI CCNNGG 4 cut(s) 428, 730, 1081, 1111
BseGI GGATG 4 cut(s) 940, 1047, 1077, 1107
BseLI CCNNNNNNNGG 3 cut(s) 468, 702, 1060
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 1 cut(s) 756
BseNI ACTGG 4 cut(s) 182, 936, 1023, 1166
BseRI GAGGAG 1 cut(s) 813
BseSI GKGCMC 1 cut(s) 994
BseXI GCAGC 1 cut(s) 118
BseYI CCCAGC 1 cut(s) 23
BsgI GTGCAG 1 cut(s) 375
BshFI GGCC 5 cut(s) 367, 585, 617, 735, 1025
BsiWI CGTACG 1 cut(s) 509
BslI CCNNNNNNNGG 3 cut(s) 468, 702, 1060
BsmI GAATGC 1 cut(s) 575
BsnI GGCC 5 cut(s) 367, 585, 617, 735, 1025
Bsp1286I GDGCHC 1 cut(s) 994
Bsp1407I TGTACA 1 cut(s) 1120
Bsp143I GATC 1 cut(s) 412
Bsp19I CCATGG 1 cut(s) 1081
BspACI CCGC 3 cut(s) 71, 498, 635
BspANI GGCC 5 cut(s) 367, 585, 617, 735, 1025
BspCNI CTCAG 1 cut(s) 755
BspHI TCATGA 1 cut(s) 909
BspQI GCTCTTC 1 cut(s) 211
BspTI CTTAAG 2 cut(s) 170, 776
BsrBI CCGCTC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 894
BsrGI TGTACA 1 cut(s) 1120
BsrI ACTGG 4 cut(s) 182, 936, 1023, 1166
BssECI CCNNGG 4 cut(s) 428, 730, 1081, 1111
BssMI GATC 1 cut(s) 412
BssT1I CCWWGG 4 cut(s) 428, 730, 1081, 1111
Bst4CI ACNGT 4 cut(s) 332, 532, 769, 843
Bst6I CTCTTC 2 cut(s) 211, 569
BstAFI CTTAAG 2 cut(s) 170, 776
BstAUI TGTACA 1 cut(s) 1120
BstBAI YACGTR 1 cut(s) 509
BstDEI CTNAG 2 cut(s) 580, 742
BstDSI CCRYGG 1 cut(s) 1081
BstEII GGTNACC 1 cut(s) 61
BstF5I GGATG 4 cut(s) 940, 1047, 1077, 1107
BstKTI GATC 1 cut(s) 415
BstMBI GATC 1 cut(s) 412
BstMWI GCNNNNNNNGC 3 cut(s) 503, 641, 1064
BstNSI RCATGY 2 cut(s) 1039, 1075
BstPI GGTNACC 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 672
BstSLI GKGCMC 1 cut(s) 994
BstV1I GCAGC 1 cut(s) 118
BsuRI GGCC 5 cut(s) 367, 585, 617, 735, 1025
BtgI CCRYGG 1 cut(s) 1081
BtrI CACGTC 1 cut(s) 814
BtsCI GGATG 4 cut(s) 940, 1047, 1077, 1107
BtsIMutI CAGTG 3 cut(s) 266, 337, 1016
CciI TCATGA 1 cut(s) 909
Csp6I GTAC 6 cut(s) 16, 151, 510, 525, 971, 1121
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 9 cut(s) 235, 410, 631, 646, 748, 910, 1036, 1072, 1082
CviQI GTAC 6 cut(s) 16, 151, 510, 525, 971, 1121
DdeI CTNAG 2 cut(s) 580, 742
DpnI GATC 1 cut(s) 414
DpnII GATC 1 cut(s) 412
DraIII CACNNNGTG 2 cut(s) 353, 998
EaeI YGGCCR 2 cut(s) 615, 1023
Eam1104I CTCTTC 2 cut(s) 211, 569
EarI CTCTTC 2 cut(s) 211, 569
Eco130I CCWWGG 4 cut(s) 428, 730, 1081, 1111
Eco147I AGGCCT 1 cut(s) 367
Eco57I CTGAAG 2 cut(s) 425, 531
Eco91I GGTNACC 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 61
EcoT14I CCWWGG 4 cut(s) 428, 730, 1081, 1111
ErhI CCWWGG 4 cut(s) 428, 730, 1081, 1111
FaeI CATG 9 cut(s) 238, 413, 634, 649, 751, 913, 1039, 1075, 1085
FatI CATG 9 cut(s) 234, 409, 630, 645, 747, 909, 1035, 1071, 1081
FauI CCCGC 1 cut(s) 628
FauNDI CATATG 1 cut(s) 754
Fnu4HI GCNGC 2 cut(s) 107, 498
FokI GGATG 4 cut(s) 947, 1034, 1064, 1094
Fsp4HI GCNGC 2 cut(s) 107, 498
FspBI CTAG 3 cut(s) 602, 927, 1055
GluI GCNGC 2 cut(s) 107, 498
GsaI CCCAGC 1 cut(s) 27
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 5 cut(s) 367, 585, 617, 735, 1025
Hin1II CATG 9 cut(s) 238, 413, 634, 649, 751, 913, 1039, 1075, 1085
HinfI GANTC 5 cut(s) 552, 590, 598, 680, 913
HphI GGTGA 4 cut(s) 255, 339, 803, 1036
Hpy166II GTNNAC 6 cut(s) 151, 263, 846, 971, 1008, 1105
Hpy188I TCNGA 4 cut(s) 36, 288, 979, 1159
Hpy188III TCNNGA 4 cut(s) 321, 416, 549, 910
Hpy8I GTNNAC 6 cut(s) 151, 263, 846, 971, 1008, 1105
Hpy99I CGWCG 1 cut(s) 204
HpyAV CCTTC 2 cut(s) 788, 799
HpyCH4III ACNGT 4 cut(s) 332, 532, 769, 843
HpyCH4IV ACGT 4 cut(s) 199, 508, 665, 813
HpyF10VI GCNNNNNNNGC 3 cut(s) 503, 641, 1064
HpyF3I CTNAG 2 cut(s) 580, 742
HpySE526I ACGT 4 cut(s) 199, 508, 665, 813
Hsp92II CATG 9 cut(s) 238, 413, 634, 649, 751, 913, 1039, 1075, 1085
Kzo9I GATC 1 cut(s) 412
LguI GCTCTTC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 1 cut(s) 118
LweI GCATC 1 cut(s) 325
MaeI CTAG 3 cut(s) 602, 927, 1055
MaeII ACGT 4 cut(s) 199, 508, 665, 813
MaeIII GTNAC 4 cut(s) 61, 76, 118, 1038
MalI GATC 1 cut(s) 414
MbiI CCGCTC 1 cut(s) 500
MboI GATC 1 cut(s) 412
MboII GAAGA 5 cut(s) 198, 240, 371, 556, 764
MhlI GDGCHC 1 cut(s) 994
MlsI TGGCCA 1 cut(s) 1025
MluCI AATT 5 cut(s) 187, 299, 306, 435, 782
MluNI TGGCCA 1 cut(s) 1025
MlyI GAGTC 2 cut(s) 546, 689
MmeI TCCRAC 1 cut(s) 943
MnlI CCTC 9 cut(s) 6, 76, 164, 474, 550, 706, 751, 791, 927
Mox20I TGGCCA 1 cut(s) 1025
MscI TGGCCA 1 cut(s) 1025
MseI TTAA 3 cut(s) 171, 195, 777
MslI CAYNNNNRTG 1 cut(s) 862
Msp20I TGGCCA 1 cut(s) 1025
MspCI CTTAAG 2 cut(s) 170, 776
Mva1269I GAATGC 1 cut(s) 575
MwoI GCNNNNNNNGC 3 cut(s) 503, 641, 1064
NcoI CCATGG 1 cut(s) 1081
NdeI CATATG 1 cut(s) 754
NdeII GATC 1 cut(s) 412
NlaIII CATG 9 cut(s) 238, 413, 634, 649, 751, 913, 1039, 1075, 1085
NmuCI GTSAC 2 cut(s) 76, 1038
NspI RCATGY 2 cut(s) 1039, 1075
PagI TCATGA 1 cut(s) 909
PceI AGGCCT 1 cut(s) 367
PciI ACATGT 1 cut(s) 1071
PciSI GCTCTTC 1 cut(s) 211
PctI GAATGC 1 cut(s) 575
PfeI GAWTC 3 cut(s) 590, 598, 913
Pfl23II CGTACG 1 cut(s) 509
PkrI GCNGC 2 cut(s) 108, 499
PleI GAGTC 2 cut(s) 546, 688
PpsI GAGTC 2 cut(s) 546, 688
Ppu21I YACGTR 1 cut(s) 509
PscI ACATGT 1 cut(s) 1071
PspEI GGTNACC 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 23
PspLI CGTACG 1 cut(s) 509
RsaI GTAC 6 cut(s) 17, 152, 511, 526, 972, 1122
RsaNI GTAC 6 cut(s) 16, 151, 510, 525, 971, 1121
RseI CAYNNNNRTG 1 cut(s) 862
SapI GCTCTTC 1 cut(s) 211
SaqAI TTAA 3 cut(s) 171, 195, 777
SatI GCNGC 2 cut(s) 107, 498
Sau3AI GATC 1 cut(s) 412
SchI GAGTC 2 cut(s) 546, 689
SduI GDGCHC 1 cut(s) 994
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 672
SmiMI CAYNNNNRTG 1 cut(s) 862
SmlI CTYRAG 2 cut(s) 170, 776
SmoI CTYRAG 2 cut(s) 170, 776
Sse9I AATT 5 cut(s) 187, 299, 306, 435, 782
SseBI AGGCCT 1 cut(s) 367
SsiI CCGC 3 cut(s) 71, 498, 635
SspMI CTAG 3 cut(s) 602, 927, 1055
StuI AGGCCT 1 cut(s) 367
StyI CCWWGG 4 cut(s) 428, 730, 1081, 1111
TaaI ACNGT 4 cut(s) 332, 532, 769, 843
TaiI ACGT 4 cut(s) 202, 511, 668, 816
TaqI TCGA 2 cut(s) 415, 689
TasI AATT 5 cut(s) 187, 299, 306, 435, 782
TatI WGTACW 3 cut(s) 524, 970, 1120
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 3 cut(s) 590, 598, 913
Tru1I TTAA 3 cut(s) 171, 195, 777
Tru9I TTAA 3 cut(s) 171, 195, 777
TscAI CASTG 3 cut(s) 273, 337, 1023
TseFI GTSAC 2 cut(s) 76, 1038
TseI GCWGC 1 cut(s) 106
Tsp45I GTSAC 2 cut(s) 76, 1038
TspDTI ATGAA 6 cut(s) 251, 299, 556, 898, 951, 1066
TspRI CASTG 3 cut(s) 273, 337, 1023
Vha464I CTTAAG 2 cut(s) 170, 776
XapI RAATTY 2 cut(s) 306, 782
XceI RCATGY 2 cut(s) 1039, 1075
XcmI CCANNNNNNNNNTGG 1 cut(s) 652
XspI CTAG 3 cut(s) 602, 927, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.