MD08G1052500.v1.1

Solute carrier family 35 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002838
Physical Location & Seq
Forward (+)
4119454 .. 4122896
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1052500.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGGTTGGAAGTACAACGCCGGTTTGGGGTTGATTGGCACAGTTGTGTTCATCTGGGTCGCTTCTGCAGAGGTTACTCAGAGAATTTTCAGTGAGTACAAACAGCCGTTCTCGCTCACGTACCTCGGTGTGTCTCTGATGGCGGTCTATCTACCCGTATCGGCTTTGAAAGATTGGATCTACAACTTGCTGCACCCAAATTTGGTTAAGAACTTTTACAATGGCATTATCGCGGGAACTTCTGGAGGGCTTGGTATTCCTCTCAGAATAAATGAAATGCCCCACAGTCCAGAAACACATGTTAGAAGCTGCCTAATTACTGACAACGATCTTAGTAAGACGGAAGAAGGGCGACCTCTAACGGATAAGAGCGAAGAAGATGAGCCGCGCTTGCTTGAACATAGTTGTCAACTGAGTCCATGGGAAGTTGCAAAGTTTGGCTTAATTCTTACTCCAGTGTGGTTTATCACAGAGTATCTGTCAAACTCTGCACTGGCGAATACCAGTGTTGCAAGTACAACTGTCCTAACTTCTACGTCGGGGCTCTTTACACTTTTCTTTGGAGCTCTTCTTCGTCAGGATTCCATAACTGTTACAAAGATGGTTGCTGTTTTTGTGAGCATGGCTGGTGTTGTCATGACCGAAGTTGGAAAAACTTGGGCCCCTGATGAACTGCGGAGCATCTCTGAGATGAGAAAGCACGCTATAATGGGACATATTTTTGGTCTTCTCTCAGCTGCGTCATATGGATTGTTTACAGTGTTACTCAAGAAATTTGCTGGATCAGAAAAGAAGGGAGATAAGGCTGATGTGCAGAAGCTTTTCGGATACATTGGTCTCTTCGCTCTGCTTGGTCTGTGGTGGCTCGTGTGGCCGCTCAATGCCATCGGGATTGAACCTTCATTTGAGTTTCCACATTCAAAATCTGTTGCCGAAGCTGTGCTGATAAATGGCTTTGTCGGGAGTGTTCTTTCGGATTATTTTTGGGCTCTTTCCGTGGTTTGGACATCTCCATTAGTTGCAACGCTGGGCATGTCCTTGACAATTCCACTCGCAATGGTAGCTGACATGGTGATTCACGGCCGCCATTTTTCTGCAGTCTACATCCTTGGTTGCATTCAGGTTTTTGCAGGATTCGTGATGGCAAACCTTTCGGACAAGTTCTCGAGCAAGCAAGAGTTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

396

Amino Acids

43.4

Weight (kDa)

6.15

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 154 - 214 1.8e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 877
AccI GTMKAC 1 cut(s) 1101
AccII CGCG 2 cut(s) 233, 388
AciI CCGC 6 cut(s) 143, 233, 386, 676, 875, 1084
AclWI GGATC 2 cut(s) 185, 790
AcoI YGGCCR 2 cut(s) 872, 1081
AcsI RAATTY 3 cut(s) 84, 199, 773
AfaI GTAC 4 cut(s) 14, 98, 122, 517
AfiI CCNNNNNNNGG 3 cut(s) 27, 202, 1002
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 4 cut(s) 169, 398, 896, 921
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 6 cut(s) 309, 566, 737, 820, 938, 1064
AluI AGCT 6 cut(s) 309, 566, 737, 820, 938, 1064
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 138, 842
AlwI GGATC 2 cut(s) 185, 790
Ama87I CYCGRG 1 cut(s) 1165
AoxI GGCC 3 cut(s) 660, 872, 1081
ApaI GGGCCC 1 cut(s) 664
ApeKI GCWGC 3 cut(s) 190, 309, 737
ApoI RAATTY 3 cut(s) 84, 199, 773
Asp700I GAANNNNTTC 1 cut(s) 821
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 2 cut(s) 660, 661
AsuHPI GGTGA 1 cut(s) 1084
AvaI CYCGRG 1 cut(s) 1165
BaeGI GKGCMC 1 cut(s) 664
BaeI ACNNNNGTAYC 2 cut(s) 820, 853
BanII GRGCYC 4 cut(s) 546, 568, 664, 991
BauI CACGAG 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 719
Bbv12I GWGCWC 1 cut(s) 568
BbvI GCAGC 3 cut(s) 177, 296, 724
BccI CCATC 4 cut(s) 133, 595, 893, 1135
BceAI ACGGC 2 cut(s) 91, 1096
BcgI CGANNNNNNTGC 2 cut(s) 1134, 1168
BciVI GTATCC 1 cut(s) 821
BcoDI GTCTC 2 cut(s) 138, 842
BfmI CTRYAG 2 cut(s) 66, 1095
BfuI GTATCC 1 cut(s) 821
BisI GCNGC 6 cut(s) 191, 310, 386, 738, 875, 1084
BlsI GCNGC 6 cut(s) 192, 311, 387, 739, 876, 1085
BmeT110I CYCGRG 1 cut(s) 1165
BmgT120I GGNCC 2 cut(s) 660, 661
BmiI GGNNCC 2 cut(s) 662, 663
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 719
BpmI CTGGAG 2 cut(s) 264, 438
BpuEI CTTGAG 1 cut(s) 752
BsaAI YACGTR 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 842
BsaJI CCNNGG 4 cut(s) 124, 419, 996, 1108
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 202, 1002
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 3 cut(s) 455, 498, 504
Bse3DI GCAATG 1 cut(s) 1062
BseDI CCNNGG 4 cut(s) 124, 419, 996, 1108
BseGI GGATG 1 cut(s) 1104
BseLI CCNNNNNNNGG 3 cut(s) 27, 202, 1002
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 5 cut(s) 92, 277, 404, 678, 747
BseNI ACTGG 3 cut(s) 455, 498, 504
BseSI GKGCMC 1 cut(s) 664
BseX3I CGGCCG 1 cut(s) 1081
BseXI GCAGC 3 cut(s) 177, 296, 724
BseYI CCCAGC 1 cut(s) 1027
BsgI GTGCAG 3 cut(s) 176, 474, 833
Bsh1236I CGCG 2 cut(s) 233, 388
Bsh1285I CGRYCG 1 cut(s) 1084
BshFI GGCC 3 cut(s) 662, 874, 1083
BsiEI CGRYCG 1 cut(s) 1084
BsiHKAI GWGCWC 1 cut(s) 568
BsiHKCI CYCGRG 1 cut(s) 1165
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 3 cut(s) 27, 202, 1002
BsmAI GTCTC 2 cut(s) 138, 842
BsmFI GGGAC 1 cut(s) 726
BsmI GAATGC 1 cut(s) 1116
BsnI GGCC 3 cut(s) 662, 874, 1083
Bso31I GGTCTC 1 cut(s) 842
BsoBI CYCGRG 1 cut(s) 1165
Bsp120I GGGCCC 1 cut(s) 660
Bsp1286I GDGCHC 4 cut(s) 546, 568, 664, 991
Bsp143I GATC 3 cut(s) 177, 328, 782
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 6 cut(s) 143, 233, 386, 676, 875, 1084
BspANI GGCC 3 cut(s) 662, 874, 1083
BspCNI CTCAG 5 cut(s) 91, 276, 405, 679, 746
BspFNI CGCG 2 cut(s) 233, 388
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 2 cut(s) 662, 663
BspMAI CTGCAG 2 cut(s) 70, 1099
BspPI GGATC 2 cut(s) 185, 790
BspQI GCTCTTC 1 cut(s) 573
BspTNI GGTCTC 1 cut(s) 842
BsrBI CCGCTC 1 cut(s) 877
BsrDI GCAATG 1 cut(s) 1062
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 3 cut(s) 455, 498, 504
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 4 cut(s) 124, 419, 996, 1108
BssMI GATC 3 cut(s) 177, 328, 782
BssSI CACGAG 1 cut(s) 866
BssT1I CCWWGG 2 cut(s) 419, 1108
Bst2BI CACGAG 1 cut(s) 866
Bst4CI ACNGT 5 cut(s) 43, 287, 523, 592, 760
Bst6I CTCTTC 2 cut(s) 573, 845
BstAPI GCANNNNNTGC 1 cut(s) 1180
BstBAI YACGTR 1 cut(s) 120
BstC8I GCNNGC 3 cut(s) 392, 702, 1172
BstDEI CTNAG 6 cut(s) 78, 263, 332, 413, 687, 733
BstDSI CCRYGG 2 cut(s) 419, 996
BstF5I GGATG 1 cut(s) 1104
BstFNI CGCG 2 cut(s) 233, 388
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 3 cut(s) 180, 331, 785
BstMAI GTCTC 2 cut(s) 138, 842
BstMBI GATC 3 cut(s) 177, 328, 782
BstMCI CGRYCG 1 cut(s) 1084
BstMWI GCNNNNNNNGC 5 cut(s) 112, 391, 871, 1061, 1180
BstNSI RCATGY 2 cut(s) 302, 1036
BstSFI CTRYAG 2 cut(s) 66, 1095
BstSLI GKGCMC 1 cut(s) 664
BstUI CGCG 2 cut(s) 233, 388
BstV1I GCAGC 3 cut(s) 177, 296, 724
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BstZI CGGCCG 1 cut(s) 1081
BsuI GTATCC 1 cut(s) 821
BsuRI GGCC 3 cut(s) 662, 874, 1083
BtgI CCRYGG 2 cut(s) 419, 996
BtsCI GGATG 1 cut(s) 1104
BtsIMutI CAGTG 5 cut(s) 97, 462, 491, 511, 765
Cac8I GCNNGC 3 cut(s) 392, 702, 1172
CciI TCATGA 1 cut(s) 636
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 660, 661
CseI GACGC 1 cut(s) 729
Csp6I GTAC 4 cut(s) 13, 97, 121, 516
CviAII CATG 6 cut(s) 299, 420, 622, 637, 1033, 1069
CviQI GTAC 4 cut(s) 13, 97, 121, 516
DdeI CTNAG 6 cut(s) 78, 263, 332, 413, 687, 733
DpnI GATC 3 cut(s) 179, 330, 784
DpnII GATC 3 cut(s) 177, 328, 782
EaeI YGGCCR 2 cut(s) 872, 1081
EagI CGGCCG 1 cut(s) 1081
Eam1104I CTCTTC 2 cut(s) 573, 845
EarI CTCTTC 2 cut(s) 573, 845
Ecl136II GAGCTC 1 cut(s) 566
EclXI CGGCCG 1 cut(s) 1081
Eco130I CCWWGG 2 cut(s) 419, 1108
Eco24I GRGCYC 4 cut(s) 546, 568, 664, 991
Eco31I GGTCTC 1 cut(s) 842
Eco52I CGGCCG 1 cut(s) 1081
Eco53kI GAGCTC 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 1165
EcoICRI GAGCTC 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 661
EcoT14I CCWWGG 2 cut(s) 419, 1108
EcoT38I GRGCYC 4 cut(s) 546, 568, 664, 991
ErhI CCWWGG 2 cut(s) 419, 1108
FaeI CATG 6 cut(s) 302, 423, 625, 640, 1036, 1072
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 1 cut(s) 726
FatI CATG 6 cut(s) 298, 419, 621, 636, 1032, 1068
FauI CCCGC 1 cut(s) 226
FauNDI CATATG 1 cut(s) 745
FblI GTMKAC 1 cut(s) 1101
Fnu4HI GCNGC 6 cut(s) 191, 310, 386, 738, 875, 1084
FokI GGATG 1 cut(s) 1091
FriOI GRGCYC 4 cut(s) 546, 568, 664, 991
Fsp4HI GCNGC 6 cut(s) 191, 310, 386, 738, 875, 1084
GlaI GCGC 1 cut(s) 389
GluI GCNGC 6 cut(s) 191, 310, 386, 738, 875, 1084
GsaI CCCAGC 1 cut(s) 1031
GsuI CTGGAG 2 cut(s) 264, 438
HaeIII GGCC 3 cut(s) 662, 874, 1083
HapII CCGG 1 cut(s) 21
HgaI GACGC 1 cut(s) 729
HhaI GCGC 1 cut(s) 390
Hin1II CATG 6 cut(s) 302, 423, 625, 640, 1036, 1072
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HincII GTYRAC 1 cut(s) 410
HindII GTYRAC 1 cut(s) 410
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 4 cut(s) 415, 581, 1075, 1134
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 1084
Hpy166II GTNNAC 3 cut(s) 410, 756, 1102
Hpy188I TCNGA 8 cut(s) 81, 138, 266, 688, 787, 827, 976, 1156
Hpy188III TCNNGA 9 cut(s) 243, 290, 578, 637, 769, 889, 961, 1138, 1165
Hpy8I GTNNAC 3 cut(s) 410, 756, 1102
Hpy99I CGWCG 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 341, 787, 909
HpyCH4III ACNGT 5 cut(s) 43, 287, 523, 592, 760
HpyCH4IV ACGT 2 cut(s) 119, 536
HpyF10VI GCNNNNNNNGC 5 cut(s) 112, 391, 871, 1061, 1180
HpyF3I CTNAG 6 cut(s) 78, 263, 332, 413, 687, 733
HpySE526I ACGT 2 cut(s) 119, 536
Hsp92II CATG 6 cut(s) 302, 423, 625, 640, 1036, 1072
HspAI GCGC 1 cut(s) 388
Kzo9I GATC 3 cut(s) 177, 328, 782
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 563, 678
Lsp1109I GCAGC 3 cut(s) 177, 296, 724
LweI GCATC 1 cut(s) 690
MaeII ACGT 2 cut(s) 119, 536
MaeIII GTNAC 3 cut(s) 73, 592, 762
MalI GATC 3 cut(s) 179, 330, 784
MbiI CCGCTC 1 cut(s) 877
MboI GATC 3 cut(s) 177, 328, 782
MboII GAAGA 7 cut(s) 356, 386, 389, 560, 563, 719, 832
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 4 cut(s) 546, 568, 664, 991
MluCI AATT 6 cut(s) 84, 199, 315, 444, 773, 1044
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 5 cut(s) 64, 134, 239, 270, 366
MroXI GAANNNNTTC 1 cut(s) 821
MseI TTAA 2 cut(s) 207, 443
MslI CAYNNNNRTG 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 1116
MvnI CGCG 2 cut(s) 233, 388
MwoI GCNNNNNNNGC 5 cut(s) 112, 391, 871, 1061, 1180
NcoI CCATGG 1 cut(s) 419
NdeI CATATG 1 cut(s) 745
NdeII GATC 3 cut(s) 177, 328, 782
NlaIII CATG 6 cut(s) 302, 423, 625, 640, 1036, 1072
NlaIV GGNNCC 2 cut(s) 662, 663
NspI RCATGY 2 cut(s) 302, 1036
OliI CACNNNNGTG 1 cut(s) 44
PaeR7I CTCGAG 1 cut(s) 1165
PagI TCATGA 1 cut(s) 636
PciI ACATGT 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 573
PctI GAATGC 1 cut(s) 1116
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 3 cut(s) 581, 1075, 1134
PkrI GCNGC 6 cut(s) 192, 311, 387, 739, 876, 1085
PleI GAGTC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 423
Ppu21I YACGTR 1 cut(s) 120
PscI ACATGT 1 cut(s) 298
Psp124BI GAGCTC 1 cut(s) 568
PspFI CCCAGC 1 cut(s) 1027
PspN4I GGNNCC 2 cut(s) 662, 663
PspOMI GGGCCC 1 cut(s) 660
PspPI GGNCC 2 cut(s) 660, 661
PstI CTGCAG 2 cut(s) 70, 1099
PsuI RGATCY 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 4 cut(s) 14, 98, 122, 517
RsaNI GTAC 4 cut(s) 13, 97, 121, 516
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 1 cut(s) 568
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 207, 443
SatI GCNGC 6 cut(s) 191, 310, 386, 738, 875, 1084
Sau3AI GATC 3 cut(s) 177, 328, 782
Sau96I GGNCC 2 cut(s) 660, 661
SchI GAGTC 1 cut(s) 424
SduI GDGCHC 4 cut(s) 546, 568, 664, 991
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 2 cut(s) 66, 1095
Sfr274I CTCGAG 1 cut(s) 1165
SlaI CTCGAG 1 cut(s) 1165
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 767, 1165
SmoI CTYRAG 2 cut(s) 767, 1165
Sse9I AATT 6 cut(s) 84, 199, 315, 444, 773, 1044
SsiI CCGC 6 cut(s) 143, 233, 386, 676, 875, 1084
SstI GAGCTC 1 cut(s) 568
StyI CCWWGG 2 cut(s) 419, 1108
TaaI ACNGT 5 cut(s) 43, 287, 523, 592, 760
TaiI ACGT 2 cut(s) 122, 539
TaqI TCGA 1 cut(s) 1166
TaqII GACCGA 1 cut(s) 656
TasI AATT 6 cut(s) 84, 199, 315, 444, 773, 1044
TatI WGTACW 3 cut(s) 12, 96, 515
TauI GCSGC 3 cut(s) 388, 877, 1086
TfiI GAWTC 3 cut(s) 581, 1075, 1134
Tru1I TTAA 2 cut(s) 207, 443
Tru9I TTAA 2 cut(s) 207, 443
TscAI CASTG 5 cut(s) 97, 462, 498, 511, 765
TseI GCWGC 3 cut(s) 190, 309, 737
TspDTI ATGAA 4 cut(s) 40, 288, 684, 891
TspGWI ACGGA 3 cut(s) 356, 377, 985
TspRI CASTG 5 cut(s) 97, 462, 498, 511, 765
XapI RAATTY 3 cut(s) 84, 199, 773
XceI RCATGY 2 cut(s) 302, 1036
XhoI CTCGAG 1 cut(s) 1165
XmiI GTMKAC 1 cut(s) 1101
XmnI GAANNNNTTC 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.