pycom15g11540

Vacuolar membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002838
Physical Location & Seq
Forward (+)
7805703 .. 7810366
4664 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g11540.3

Sequence Viewer

Length: 1191 bp
ATGATGGGGTGGAGGTACAGAGCTGGGTTGTTCCTAATTGCTGCTGTTGTTGTCATTTGGGTCACCTCTGCAGAAGTTACCCAGGGTATATTTACGGACTATAAGCAGCCATTTGCAGTAACATATCTTGGAGCTTCTCTTATGGTAGTTTACATCCCAATAGCATTCATTAAGGATTGGTTGTGTAATTTTCTAAAACGCCGCTCCTCTAAAAGTGGTAAAAATGCAGAAAGCAGCATGAATGAGTTTTCTGCTGGATTTAGTTCTACTCTAAAACTCAATGGAGTGAAAGACTTCGAATTGGAAATTCATGGGTCCTTGACCAGAAAAGACAGTGATGCAGACCTTTCACCTCATGCGGAAGAACAGGCTTTGGTTTCTAAATACAAAGATGATTTAAATGTGCTGAAACATGATAAAGAGGTTACTACGAGGCAAATTGCTACTTATGGGTTTTACATTGCCCCTCTCTGGTTTATTACGGAGTATTTTTCAAATGCTGCTCTCGCGCGTACAAGTGTTGCAAGTACAACAGTATTATCCTCGACGTCAGGACTCTTTACTCTTTTTGTAGGTGCATTCTTGGGCGAAGATTCTTTAAATGTGGCAAAGGTGGTCGCTGTCTTTGTTAGCATGGCTGGTGTAGCTATGACAACTCTGGGCAAAACATGGGCTACGGATGAGTCACAACTGAGTGCCGCCAACGGGAAACGCTCTCTTGTTGGAGATATGTTTGGTCTTCTCTCAGCCATGTCATATGGTCTATTCACCGTTCTTCTTAAAAAGTTTGCGGGCGAAGGAGGAGAAAGGGTTGACGTGCAAAAGTTGTTTGGGTATATTGGATTGTTTACACTAGTATCGTTATGGTGGCTTATTTGGCCGTTGACAGCATTGGGCATTGAGTCCAAGTTTATGATTCCTCACTCTACTAAATTGGAAGAAGTTGTTATTGCCAACGGCTTTATTGGAAGTGTACTCTCCGACTACTTCTGGGCACTAAGTGTTGTGTGGACAACTCCACTCGTTGCCACCTTGGGCATGTCGCTCACCATCCCCCTTGCTATGGTTGCTGACATGTTCATCCATGGCCGTCATTATTCAGTAGTTTACATACTTGGTTCTGCTCAGGTATTTGCAGGATTTGTAATAGCTAACCTTTCGGATTGGTGCTCGAAAAAGTTGGGATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

397

Amino Acids

43.41

Weight (kDa)

7.02

Isoelectric Point (pI)

24.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 551
AccBSI CCGCTC 1 cut(s) 204
AccII CGCG 2 cut(s) 509, 511
AciI CCGC 4 cut(s) 202, 359, 699, 791
AcoI YGGCCR 2 cut(s) 878, 1087
AcsI RAATTY 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 548
AdeI CACNNNGTG 1 cut(s) 1001
AfaI GTAC 4 cut(s) 17, 514, 529, 975
AfiI CCNNNNNNNGG 3 cut(s) 471, 705, 1063
AflIII ACRYGT 1 cut(s) 1074
AgsI TTSAA 1 cut(s) 495
AhlI ACTAGT 1 cut(s) 853
AjiI CACGTC 1 cut(s) 817
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 4 cut(s) 23, 134, 647, 1151
AluI AGCT 4 cut(s) 23, 134, 647, 1151
Alw21I GWGCWC 1 cut(s) 1172
AoxI GGCC 2 cut(s) 878, 1087
ApeKI GCWGC 4 cut(s) 41, 106, 234, 500
ApoI RAATTY 1 cut(s) 306
Asp700I GAANNNNTTC 1 cut(s) 293
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 1 cut(s) 315
AsuHPI GGTGA 4 cut(s) 55, 342, 760, 1039
AsuII TTCGAA 1 cut(s) 297
AvaII GGWCC 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 997
BaeI ACNNNNGTAYC 2 cut(s) 840, 873
BbsI GAAGAC 1 cut(s) 731
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 4 cut(s) 28, 118, 246, 487
BccI CCATC 1 cut(s) 1058
BceAI ACGGC 3 cut(s) 865, 973, 1074
BciT130I CCWGG 1 cut(s) 83
BcuI ACTAGT 1 cut(s) 853
BfaI CTAG 1 cut(s) 854
BfmI CTRYAG 1 cut(s) 69
BisI GCNGC 6 cut(s) 42, 107, 202, 235, 501, 699
BlsI GCNGC 6 cut(s) 43, 108, 203, 236, 502, 700
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 315
BmgBI CACGTC 1 cut(s) 817
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 731
Bpu10I CCTNAGC 1 cut(s) 1125
Bpu14I TTCGAA 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 548
BsaJI CCNNGG 4 cut(s) 81, 82, 1032, 1084
BsaXI ACNNNNNCTCC 2 cut(s) 795, 825
Bsc4I CCNNNNNNNGG 3 cut(s) 471, 705, 1063
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 4 cut(s) 81, 82, 1032, 1084
BseGI GGATG 4 cut(s) 153, 685, 1050, 1080
BseLI CCNNNNNNNGG 3 cut(s) 471, 705, 1063
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 3 cut(s) 683, 759, 1139
BseRI GAGGAG 2 cut(s) 196, 816
BseSI GKGCMC 1 cut(s) 997
BseXI GCAGC 4 cut(s) 28, 118, 246, 487
BseYI CCCAGC 1 cut(s) 23
Bsh1236I CGCG 2 cut(s) 509, 511
BshFI GGCC 2 cut(s) 880, 1089
BsiHKAI GWGCWC 1 cut(s) 1172
BslI CCNNNNNNNGG 3 cut(s) 471, 705, 1063
BsmI GAATGC 2 cut(s) 164, 578
BsnI GGCC 2 cut(s) 880, 1089
Bsp119I TTCGAA 1 cut(s) 297
Bsp1286I GDGCHC 2 cut(s) 997, 1172
Bsp19I CCATGG 1 cut(s) 1084
BspACI CCGC 4 cut(s) 202, 359, 699, 791
BspANI GGCC 2 cut(s) 880, 1089
BspCNI CTCAG 3 cut(s) 684, 758, 1138
BspFNI CGCG 2 cut(s) 509, 511
BspLI GGNNCC 1 cut(s) 316
BspMAI CTGCAG 1 cut(s) 73
BspT104I TTCGAA 1 cut(s) 297
BsrBI CCGCTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 459
BssECI CCNNGG 4 cut(s) 81, 82, 1032, 1084
BssNI GRCGYC 1 cut(s) 548
BssT1I CCWWGG 2 cut(s) 1032, 1084
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 3 cut(s) 335, 535, 772
BstACI GRCGYC 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 297
BstC8I GCNNGC 1 cut(s) 793
BstDEI CTNAG 4 cut(s) 692, 745, 998, 1125
BstDSI CCRYGG 1 cut(s) 1084
BstEII GGTNACC 1 cut(s) 61
BstF5I GGATG 4 cut(s) 153, 685, 1050, 1080
BstFNI CGCG 2 cut(s) 509, 511
BstHHI GCGC 1 cut(s) 511
BstMWI GCNNNNNNNGC 4 cut(s) 506, 644, 877, 1067
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 2 cut(s) 1042, 1078
BstPI GGTNACC 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 1 cut(s) 69
BstSLI GKGCMC 1 cut(s) 997
BstUI CGCG 2 cut(s) 509, 511
BstV1I GCAGC 4 cut(s) 28, 118, 246, 487
BstV2I GAAGAC 1 cut(s) 731
BsuRI GGCC 2 cut(s) 880, 1089
BtgI CCRYGG 1 cut(s) 1084
BtrI CACGTC 1 cut(s) 817
BtsCI GGATG 4 cut(s) 153, 685, 1050, 1080
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 793
CfoI GCGC 1 cut(s) 511
Cfr13I GGNCC 1 cut(s) 315
Csp6I GTAC 4 cut(s) 16, 513, 528, 974
CviQI GTAC 4 cut(s) 16, 513, 528, 974
DdeI CTNAG 4 cut(s) 692, 745, 998, 1125
DraI TTTAAA 2 cut(s) 399, 600
DraIII CACNNNGTG 1 cut(s) 1001
EaeI YGGCCR 2 cut(s) 878, 1087
Eco130I CCWWGG 2 cut(s) 1032, 1084
Eco47I GGWCC 1 cut(s) 315
Eco91I GGTNACC 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 315
EcoO65I GGTNACC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 2 cut(s) 1032, 1084
ErhI CCWWGG 2 cut(s) 1032, 1084
FauI CCCGC 1 cut(s) 784
FauNDI CATATG 1 cut(s) 757
Fnu4HI GCNGC 6 cut(s) 42, 107, 202, 235, 501, 699
FokI GGATG 4 cut(s) 140, 692, 1037, 1067
Fsp4HI GCNGC 6 cut(s) 42, 107, 202, 235, 501, 699
FspBI CTAG 1 cut(s) 854
GlaI GCGC 1 cut(s) 510
GluI GCNGC 6 cut(s) 42, 107, 202, 235, 501, 699
GsaI CCCAGC 1 cut(s) 27
HaeIII GGCC 2 cut(s) 880, 1089
HhaI GCGC 1 cut(s) 511
Hin1I GRCGYC 1 cut(s) 548
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HincII GTYRAC 2 cut(s) 814, 885
HindII GTYRAC 2 cut(s) 814, 885
HinfI GANTC 5 cut(s) 555, 593, 683, 902, 916
HphI GGTGA 4 cut(s) 55, 342, 760, 1039
Hpy166II GTNNAC 7 cut(s) 151, 814, 849, 885, 974, 1011, 1108
Hpy188I TCNGA 2 cut(s) 982, 1162
Hpy188III TCNNGA 1 cut(s) 552
Hpy8I GTNNAC 7 cut(s) 151, 814, 849, 885, 974, 1011, 1108
Hpy99I CGWCG 1 cut(s) 550
HpyAV CCTTC 1 cut(s) 791
HpyCH4III ACNGT 3 cut(s) 335, 535, 772
HpyCH4IV ACGT 2 cut(s) 548, 816
HpyCH4V TGCA 8 cut(s) 71, 116, 227, 341, 524, 578, 820, 1136
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 644, 877, 1067
HpyF3I CTNAG 4 cut(s) 692, 745, 998, 1125
HpySE526I ACGT 2 cut(s) 548, 816
Hsp92I GRCGYC 1 cut(s) 548
HspAI GCGC 1 cut(s) 509
LmnI GCTCC 2 cut(s) 131, 209
Lsp1109I GCAGC 4 cut(s) 28, 118, 246, 487
LweI GCATC 1 cut(s) 328
MaeI CTAG 1 cut(s) 854
MaeII ACGT 2 cut(s) 548, 816
MaeIII GTNAC 5 cut(s) 61, 76, 118, 424, 684
MbiI CCGCTC 1 cut(s) 204
MboII GAAGA 5 cut(s) 374, 602, 731, 767, 950
MhlI GDGCHC 2 cut(s) 997, 1172
MluCI AATT 6 cut(s) 36, 187, 299, 306, 438, 932
MlyI GAGTC 3 cut(s) 549, 692, 911
MmeI TCCRAC 2 cut(s) 703, 1005
MroXI GAANNNNTTC 1 cut(s) 293
MseI TTAA 4 cut(s) 171, 398, 599, 780
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 2 cut(s) 164, 578
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 2 cut(s) 509, 511
MwoI GCNNNNNNNGC 4 cut(s) 506, 644, 877, 1067
NcoI CCATGG 1 cut(s) 1084
NdeI CATATG 1 cut(s) 757
NlaIV GGNNCC 1 cut(s) 316
NmuCI GTSAC 2 cut(s) 61, 684
NspI RCATGY 2 cut(s) 1042, 1078
NspV TTCGAA 1 cut(s) 297
PasI CCCWGGG 1 cut(s) 82
PciI ACATGT 1 cut(s) 1074
PctI GAATGC 2 cut(s) 164, 578
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 593, 916
PkrI GCNGC 6 cut(s) 43, 108, 203, 236, 502, 700
PleI GAGTC 3 cut(s) 549, 691, 910
PpsI GAGTC 3 cut(s) 549, 691, 910
PpuMI RGGWCCY 1 cut(s) 315
PscI ACATGT 1 cut(s) 1074
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 81
PspEI GGTNACC 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 23
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 1 cut(s) 315
PspPPI RGGWCCY 1 cut(s) 315
PstI CTGCAG 1 cut(s) 73
RsaI GTAC 4 cut(s) 17, 514, 529, 975
RsaNI GTAC 4 cut(s) 16, 513, 528, 974
SaqAI TTAA 4 cut(s) 171, 398, 599, 780
SatI GCNGC 6 cut(s) 42, 107, 202, 235, 501, 699
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 3 cut(s) 549, 692, 911
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 2 cut(s) 997, 1172
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 69
SfuI TTCGAA 1 cut(s) 297
SinI GGWCC 1 cut(s) 315
SmiI ATTTAAAT 1 cut(s) 399
SpeI ACTAGT 1 cut(s) 853
Sse9I AATT 6 cut(s) 36, 187, 299, 306, 438, 932
SsiI CCGC 4 cut(s) 202, 359, 699, 791
SspMI CTAG 1 cut(s) 854
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 2 cut(s) 1032, 1084
SwaI ATTTAAAT 1 cut(s) 399
TaaI ACNGT 3 cut(s) 335, 535, 772
TaiI ACGT 2 cut(s) 551, 819
TaqI TCGA 3 cut(s) 297, 545, 1172
TasI AATT 6 cut(s) 36, 187, 299, 306, 438, 932
TatI WGTACW 2 cut(s) 527, 973
TauI GCSGC 2 cut(s) 204, 701
TfiI GAWTC 2 cut(s) 593, 916
Tru1I TTAA 4 cut(s) 171, 398, 599, 780
Tru9I TTAA 4 cut(s) 171, 398, 599, 780
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 2 cut(s) 61, 684
TseI GCWGC 4 cut(s) 41, 106, 234, 500
Tsp45I GTSAC 2 cut(s) 61, 684
TspDTI ATGAA 4 cut(s) 157, 254, 299, 1069
TspGWI ACGGA 3 cut(s) 110, 497, 692
TspRI CASTG 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 315
XapI RAATTY 1 cut(s) 306
XceI RCATGY 2 cut(s) 1042, 1078
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 1 cut(s) 854
ZraI GACGTC 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.