Rh6DG384900

Vacuolar membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002838
Physical Location & Seq
Reverse (-)
57789110 .. 57793709
4600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG384900.1

Sequence Viewer

Length: 1185 bp
ATGGGTTGGAGATACAGAGCTGGGTTGCTTCTAATTGGTGCTGTGGTTATCATTTGGGTTACCTCTGCAGAAGTCACACAAGATATCTATGAAAAGTATAAGCAGCCATTTGCAGTAACATATCTTGGAGCTTCTCTTATGGTAGTGTACCTCCCAATAGCTTTCCTTAAGGACTGGTTGTGTAATTTACTTAAACGTCGCTCTTCTAAAAGTGGCAAAAATGCAGAAAGCATGAATGAGTATTCTGCTGGATTTAGTTCACCACTAAAACACAATGGAGTGCAGAAAGATTTTGAATTGGAAATTCATACCTTGTCCAGAAAAGACAGTGATGCAGACCTTTCACCTTGTGCAGAAGAAAGACCTTTGGTTTCTAAACTCAAAGACAATTTAAATGTGCTGAAACATGACAGAGAGCTCACACCAAGGGAAATTGCTACTTATGGATTTTACCTTGCCCCTCTTTGGTTTTTTACAGAGTATTTTGCAAATGCTGCTCTCGCGCGTACGAGTGTTGCAAGTACAACAGTCTTATCCTCAACTTCAGGACTCTTTACACTCTTTATTGGTGCATTCTTGGGCCAAGATTCTTTAAATATAGCAAAAGTGGTGGCTGTCTTTGTCAGCATGGCGGGTGTAGCCATGACAACTATGGGAAAAACGTGGGCTACAGATGAGTCACAATCGAGTGCCTCCAATGGGAAACACTCTCTCGTTGGAGATGTTTTCGGCCTTTCCTCAGCTATGTCATACGGTCTATTTACAGTCCTTCTTAAGAAATTTGGTGGTGAAGGAGGAGAGAAGGCTGACGTGCAAAAATTGTTTGGGTATATTGGATTGTTTACGCTCGTAGCATTATGGTGGCTGGTTTTGCCATTGACAGCAATGGGCATTGAACCAAAGTTCATGATTCCTCACTCTGCTAAATTAGATGAACTTGTTCTTGCCAATGGCTTTGTCGGAAGTGTACTTTCTGATTACTTTTGGGCACTATGTGTTGTGTGGACAACTCCACTGGTGGCCACTCTGGGCATGTCGCTCACCATCCCCCTAGCTATGGTGGCTGACATGTTTATCCATGGTCGTCATTATTCGGCAGTTTACATTCTTGGCTCTGTACAGGTATTTGCAGGATTTGTAATAGCTAATCTTTCAGATTGGTTCTCAAAGAAGTTGGGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

394

Amino Acids

43.2

Weight (kDa)

7.69

Isoelectric Point (pI)

28.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 141 - 217 1.9e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 503, 505
AciI CCGC 1 cut(s) 632
AcoI YGGCCR 1 cut(s) 1020
AcsI RAATTY 2 cut(s) 303, 779
AcuI CTGAAG 1 cut(s) 528
AdeI CACNNNGTG 2 cut(s) 350, 995
AfaI GTAC 5 cut(s) 149, 508, 523, 969, 1119
AfiI CCNNNNNNNGG 3 cut(s) 465, 699, 1057
AflII CTTAAG 2 cut(s) 167, 773
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 2 cut(s) 296, 896
AjiI CACGTC 1 cut(s) 811
AluBI AGCT 7 cut(s) 20, 131, 161, 418, 743, 1055, 1145
AluI AGCT 7 cut(s) 20, 131, 161, 418, 743, 1055, 1145
Alw21I GWGCWC 1 cut(s) 420
AoxI GGCC 3 cut(s) 580, 730, 1020
ApeKI GCWGC 2 cut(s) 103, 494
ApoI RAATTY 2 cut(s) 303, 779
Asp700I GAANNNNTTC 1 cut(s) 939
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 580
AsuHPI GGTGA 4 cut(s) 252, 336, 800, 1033
BaeGI GKGCMC 1 cut(s) 991
BalI TGGCCA 1 cut(s) 1022
BanII GRGCYC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 420
BbvCI CCTCAGC 1 cut(s) 739
BbvI GCAGC 2 cut(s) 115, 481
BccI CCATC 1 cut(s) 1052
BfaI CTAG 1 cut(s) 1052
BfmI CTRYAG 2 cut(s) 66, 669
BfrI CTTAAG 2 cut(s) 167, 773
BisI GCNGC 2 cut(s) 104, 495
BlsI GCNGC 2 cut(s) 105, 496
BmgBI CACGTC 1 cut(s) 811
BmgT120I GGNCC 1 cut(s) 580
BmsI GCATC 1 cut(s) 322
Bpu10I CCTNAGC 1 cut(s) 739
BsaJI CCNNGG 2 cut(s) 425, 1078
Bsc4I CCNNNNNNNGG 3 cut(s) 465, 699, 1057
Bse1I ACTGG 2 cut(s) 179, 1020
Bse3DI GCAATG 1 cut(s) 891
BseDI CCNNGG 2 cut(s) 425, 1078
BseGI GGATG 1 cut(s) 1044
BseLI CCNNNNNNNGG 3 cut(s) 465, 699, 1057
BseMI GCAATG 1 cut(s) 891
BseMII CTCAG 1 cut(s) 753
BseNI ACTGG 2 cut(s) 179, 1020
BseRI GAGGAG 1 cut(s) 810
BseSI GKGCMC 1 cut(s) 991
BseXI GCAGC 2 cut(s) 115, 481
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 2 cut(s) 302, 372
Bsh1236I CGCG 2 cut(s) 503, 505
BshFI GGCC 3 cut(s) 582, 732, 1022
BsiHKAI GWGCWC 1 cut(s) 420
BsiWI CGTACG 1 cut(s) 506
BslI CCNNNNNNNGG 3 cut(s) 465, 699, 1057
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 3 cut(s) 582, 732, 1022
Bsp1286I GDGCHC 2 cut(s) 420, 991
Bsp1407I TGTACA 1 cut(s) 1117
Bsp19I CCATGG 1 cut(s) 1078
BspACI CCGC 1 cut(s) 632
BspANI GGCC 3 cut(s) 582, 732, 1022
BspCNI CTCAG 1 cut(s) 752
BspFNI CGCG 2 cut(s) 503, 505
BspHI TCATGA 1 cut(s) 906
BspMAI CTGCAG 1 cut(s) 70
BspQI GCTCTTC 1 cut(s) 208
BspTI CTTAAG 2 cut(s) 167, 773
BsrDI GCAATG 1 cut(s) 891
BsrGI TGTACA 1 cut(s) 1117
BsrI ACTGG 2 cut(s) 179, 1020
BssECI CCNNGG 2 cut(s) 425, 1078
BssT1I CCWWGG 2 cut(s) 425, 1078
Bst4CI ACNGT 4 cut(s) 329, 529, 755, 766
Bst6I CTCTTC 1 cut(s) 208
BstAFI CTTAAG 2 cut(s) 167, 773
BstAPI GCANNNNNTGC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 1117
BstDEI CTNAG 1 cut(s) 739
BstDSI CCRYGG 1 cut(s) 1078
BstEII GGTNACC 1 cut(s) 58
BstF5I GGATG 1 cut(s) 1044
BstFNI CGCG 2 cut(s) 503, 505
BstHHI GCGC 1 cut(s) 505
BstMWI GCNNNNNNNGC 5 cut(s) 494, 500, 638, 871, 1061
BstNSI RCATGY 2 cut(s) 1036, 1072
BstPI GGTNACC 1 cut(s) 58
BstSFI CTRYAG 2 cut(s) 66, 669
BstSLI GKGCMC 1 cut(s) 991
BstUI CGCG 2 cut(s) 503, 505
BstV1I GCAGC 2 cut(s) 115, 481
BsuRI GGCC 3 cut(s) 582, 732, 1022
BtgI CCRYGG 1 cut(s) 1078
BtrI CACGTC 1 cut(s) 811
BtsCI GGATG 1 cut(s) 1044
BtsIMutI CAGTG 2 cut(s) 334, 1013
CciI TCATGA 1 cut(s) 906
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 580
Csp6I GTAC 5 cut(s) 148, 507, 522, 968, 1118
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 8 cut(s) 232, 407, 628, 643, 907, 1033, 1069, 1079
CviQI GTAC 5 cut(s) 148, 507, 522, 968, 1118
DdeI CTNAG 1 cut(s) 739
DraI TTTAAA 2 cut(s) 393, 594
DraIII CACNNNGTG 2 cut(s) 350, 995
EaeI YGGCCR 1 cut(s) 1020
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 418
Eco130I CCWWGG 2 cut(s) 425, 1078
Eco24I GRGCYC 1 cut(s) 420
Eco32I GATATC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 418
EcoO65I GGTNACC 1 cut(s) 58
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 425, 1078
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 2 cut(s) 425, 1078
FaeI CATG 8 cut(s) 235, 410, 631, 646, 910, 1036, 1072, 1082
FatI CATG 8 cut(s) 231, 406, 627, 642, 906, 1032, 1068, 1078
FauI CCCGC 1 cut(s) 625
Fnu4HI GCNGC 2 cut(s) 104, 495
FokI GGATG 1 cut(s) 1031
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 2 cut(s) 104, 495
FspBI CTAG 1 cut(s) 1052
GlaI GCGC 1 cut(s) 504
GluI GCNGC 2 cut(s) 104, 495
GsaI CCCAGC 1 cut(s) 24
HaeIII GGCC 3 cut(s) 582, 732, 1022
HhaI GCGC 1 cut(s) 505
Hin1II CATG 8 cut(s) 235, 410, 631, 646, 910, 1036, 1072, 1082
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HinfI GANTC 4 cut(s) 549, 587, 677, 910
HphI GGTGA 4 cut(s) 252, 336, 800, 1033
Hpy166II GTNNAC 6 cut(s) 148, 260, 843, 968, 1005, 1102
Hpy188I TCNGA 3 cut(s) 962, 976, 1156
Hpy188III TCNNGA 3 cut(s) 318, 546, 907
Hpy8I GTNNAC 6 cut(s) 148, 260, 843, 968, 1005, 1102
Hpy99I CGWCG 1 cut(s) 201
HpyAV CCTTC 3 cut(s) 779, 785, 796
HpyCH4III ACNGT 4 cut(s) 329, 529, 755, 766
HpyCH4IV ACGT 3 cut(s) 196, 662, 810
HpyF10VI GCNNNNNNNGC 5 cut(s) 494, 500, 638, 871, 1061
HpyF3I CTNAG 1 cut(s) 739
HpySE526I ACGT 3 cut(s) 196, 662, 810
Hsp92II CATG 8 cut(s) 235, 410, 631, 646, 910, 1036, 1072, 1082
HspAI GCGC 1 cut(s) 503
LguI GCTCTTC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 2 cut(s) 115, 481
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 1052
MaeII ACGT 3 cut(s) 196, 662, 810
MaeIII GTNAC 4 cut(s) 58, 73, 115, 678
MboII GAAGA 2 cut(s) 195, 368
MhlI GDGCHC 2 cut(s) 420, 991
MlsI TGGCCA 1 cut(s) 1022
MluCI AATT 9 cut(s) 33, 184, 296, 303, 388, 432, 779, 818, 926
MluNI TGGCCA 1 cut(s) 1022
MlyI GAGTC 2 cut(s) 543, 686
MmeI TCCRAC 2 cut(s) 697, 940
MnlI CCTC 8 cut(s) 73, 161, 471, 547, 703, 748, 788, 924
Mox20I TGGCCA 1 cut(s) 1022
MroXI GAANNNNTTC 1 cut(s) 939
MscI TGGCCA 1 cut(s) 1022
MseI TTAA 5 cut(s) 168, 192, 392, 593, 774
MslI CAYNNNNRTG 1 cut(s) 859
Msp20I TGGCCA 1 cut(s) 1022
MspCI CTTAAG 2 cut(s) 167, 773
Mva1269I GAATGC 1 cut(s) 572
MvnI CGCG 2 cut(s) 503, 505
MwoI GCNNNNNNNGC 5 cut(s) 494, 500, 638, 871, 1061
NcoI CCATGG 1 cut(s) 1078
NlaIII CATG 8 cut(s) 235, 410, 631, 646, 910, 1036, 1072, 1082
NmuCI GTSAC 2 cut(s) 73, 678
NspI RCATGY 2 cut(s) 1036, 1072
PagI TCATGA 1 cut(s) 906
PciI ACATGT 1 cut(s) 1068
PciSI GCTCTTC 1 cut(s) 208
PctI GAATGC 1 cut(s) 572
PdmI GAANNNNTTC 1 cut(s) 939
PfeI GAWTC 2 cut(s) 587, 910
Pfl23II CGTACG 1 cut(s) 506
PkrI GCNGC 2 cut(s) 105, 496
PleI GAGTC 2 cut(s) 543, 685
PpsI GAGTC 2 cut(s) 543, 685
PscI ACATGT 1 cut(s) 1068
Psp124BI GAGCTC 1 cut(s) 420
PspEI GGTNACC 1 cut(s) 58
PspFI CCCAGC 1 cut(s) 20
PspLI CGTACG 1 cut(s) 506
PspPI GGNCC 1 cut(s) 580
PstI CTGCAG 1 cut(s) 70
RsaI GTAC 5 cut(s) 149, 508, 523, 969, 1119
RsaNI GTAC 5 cut(s) 148, 507, 522, 968, 1118
RseI CAYNNNNRTG 1 cut(s) 859
SacI GAGCTC 1 cut(s) 420
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 5 cut(s) 168, 192, 392, 593, 774
SatI GCNGC 2 cut(s) 104, 495
Sau96I GGNCC 1 cut(s) 580
SchI GAGTC 2 cut(s) 543, 686
SduI GDGCHC 2 cut(s) 420, 991
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 2 cut(s) 66, 669
SmiI ATTTAAAT 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 859
SmlI CTYRAG 2 cut(s) 167, 773
SmoI CTYRAG 2 cut(s) 167, 773
Sse9I AATT 9 cut(s) 33, 184, 296, 303, 388, 432, 779, 818, 926
SsiI CCGC 1 cut(s) 632
SspMI CTAG 1 cut(s) 1052
SstI GAGCTC 1 cut(s) 420
StyI CCWWGG 2 cut(s) 425, 1078
SwaI ATTTAAAT 1 cut(s) 393
TaaI ACNGT 4 cut(s) 329, 529, 755, 766
TaiI ACGT 3 cut(s) 199, 665, 813
TaqI TCGA 1 cut(s) 686
TasI AATT 9 cut(s) 33, 184, 296, 303, 388, 432, 779, 818, 926
TatI WGTACW 3 cut(s) 521, 967, 1117
TfiI GAWTC 2 cut(s) 587, 910
Tru1I TTAA 5 cut(s) 168, 192, 392, 593, 774
Tru9I TTAA 5 cut(s) 168, 192, 392, 593, 774
TscAI CASTG 2 cut(s) 334, 1020
TseFI GTSAC 2 cut(s) 73, 678
TseI GCWGC 2 cut(s) 103, 494
Tsp45I GTSAC 2 cut(s) 73, 678
TspDTI ATGAA 5 cut(s) 105, 248, 296, 895, 948
TspRI CASTG 2 cut(s) 334, 1020
Vha464I CTTAAG 2 cut(s) 167, 773
XapI RAATTY 2 cut(s) 303, 779
XceI RCATGY 2 cut(s) 1036, 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 1 cut(s) 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.