Rh6BG392500

Vacuolar membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002838
Physical Location & Seq
Reverse (-)
62087733 .. 62090311
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG392500.1

Sequence Viewer

Length: 1047 bp
ATGGTAGTGTACCTCCCAATAGCTTTCCTTAAGGACTGGTTGTGTAATTTACTTAAACGTCGCTCTTCTAAAAGTGGCAAAAATGCAGAAAGCATGAATGAGTATTCTGCTGGATTTAGTTCACCACTAAAACACAATGGAGTGCAGAAAGATTTTGAATTGGAAATTCATACCTTGTCCAGAAAAGACAGTGATGCAGACCTTTCACCTTGTGCAGAAGAAAGGCCTTTGGTTTCTAAACTCAAAGACAATTTAAATGTGCTGAAACATGACAGAGAGCTCACACCAAGGGAAATTGCTACTTATGGATTTTACCTTGCCCCTCTTTGGTTTTTTACAGAGTATTTTGCAAATGCTGCTCTCGCGCGTACGAGTGTTGCAAGTACAACAGTCTTATCCTCAACTTCAGGACTCTTTACACTCTTTATTGGTGCATTCTTGGGCCAAGATTCTTTAAATATAGCAAAAGTGGTGGCTGTCTTTGTCAGCATGGCGGGTGTAGCCATGACAACTATGGGAAAAACGTGGGCTACAGATGAGTCACAATCGAGTGCCTCCAATGGGAAACACTCTCTCGTTGGAGATGTTTTCGGCCTTTCCTCAGCTATGTCATACGGTCTATTTACAGTCCTTCTTAAGAAATTTGGTGGTGAAGGAGGAGAGAAGGCTGACGTGCAAAAATTGTTTGGGTATATTGGATTGTTTACGCTCGTAGCATTATGGTGGCTGGTTTTGCCATTGACAGCAATGGGCATTGAACCAAAGTTCATGATTCCTCACTCTGCTAAATTAGATGAACTTGTTCTTGCCAATGGCTTTGTCGGAAGTGTACTTTCTGATTACTTTTGGGCACTATGTGTTGTGTGGACAACTCCTCTGGTGGCCACTCTGGGCATGTCGCTCACCATCCCCCTAGCAATGGTGGCTGACATGTTTATCCATGGTCGTCATTATTCGGCAGTTTACATTCTTGGCTCTGTACAGGTATTTGCAGGATTTGTAATAGCTAATCTTTCAGATTGGTTCTCAAAGAAGTTGGGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

348

Amino Acids

38.02

Weight (kDa)

7.05

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 94 - 171 1.5e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 365, 367
AciI CCGC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 882
AcsI RAATTY 2 cut(s) 165, 641
AcuI CTGAAG 1 cut(s) 390
AdeI CACNNNGTG 2 cut(s) 212, 857
AfaI GTAC 5 cut(s) 11, 370, 385, 831, 981
AfiI CCNNNNNNNGG 3 cut(s) 327, 561, 919
AflII CTTAAG 2 cut(s) 29, 635
AflIII ACRYGT 1 cut(s) 930
AgsI TTSAA 2 cut(s) 158, 758
AjiI CACGTC 1 cut(s) 673
AluBI AGCT 4 cut(s) 23, 280, 605, 1007
AluI AGCT 4 cut(s) 23, 280, 605, 1007
Alw21I GWGCWC 1 cut(s) 282
AoxI GGCC 4 cut(s) 224, 442, 592, 882
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 165, 641
Asp700I GAANNNNTTC 1 cut(s) 801
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 442
AsuHPI GGTGA 4 cut(s) 114, 198, 662, 895
BaeGI GKGCMC 1 cut(s) 853
BalI TGGCCA 1 cut(s) 884
BanII GRGCYC 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 601
BbvI GCAGC 1 cut(s) 343
BccI CCATC 1 cut(s) 914
BfaI CTAG 1 cut(s) 914
BfmI CTRYAG 1 cut(s) 531
BfrI CTTAAG 2 cut(s) 29, 635
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 1 cut(s) 442
BmsI GCATC 1 cut(s) 184
Bpu10I CCTNAGC 1 cut(s) 601
BsaJI CCNNGG 2 cut(s) 287, 940
Bsc4I CCNNNNNNNGG 3 cut(s) 327, 561, 919
Bse1I ACTGG 1 cut(s) 41
Bse3DI GCAATG 2 cut(s) 753, 924
BseDI CCNNGG 2 cut(s) 287, 940
BseGI GGATG 1 cut(s) 906
BseLI CCNNNNNNNGG 3 cut(s) 327, 561, 919
BseMI GCAATG 2 cut(s) 753, 924
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 1 cut(s) 41
BseRI GAGGAG 2 cut(s) 672, 864
BseSI GKGCMC 1 cut(s) 853
BseXI GCAGC 1 cut(s) 343
BsgI GTGCAG 2 cut(s) 164, 234
Bsh1236I CGCG 2 cut(s) 365, 367
BshFI GGCC 4 cut(s) 226, 444, 594, 884
BsiHKAI GWGCWC 1 cut(s) 282
BsiWI CGTACG 1 cut(s) 368
BslI CCNNNNNNNGG 3 cut(s) 327, 561, 919
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 4 cut(s) 226, 444, 594, 884
Bsp1286I GDGCHC 2 cut(s) 282, 853
Bsp1407I TGTACA 1 cut(s) 979
Bsp19I CCATGG 1 cut(s) 940
BspACI CCGC 1 cut(s) 494
BspANI GGCC 4 cut(s) 226, 444, 594, 884
BspCNI CTCAG 1 cut(s) 614
BspFNI CGCG 2 cut(s) 365, 367
BspHI TCATGA 1 cut(s) 768
BspQI GCTCTTC 1 cut(s) 70
BspTI CTTAAG 2 cut(s) 29, 635
BsrDI GCAATG 2 cut(s) 753, 924
BsrGI TGTACA 1 cut(s) 979
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 287, 940
BssT1I CCWWGG 2 cut(s) 287, 940
Bst4CI ACNGT 4 cut(s) 191, 391, 617, 628
Bst6I CTCTTC 1 cut(s) 70
BstAFI CTTAAG 2 cut(s) 29, 635
BstAPI GCANNNNNTGC 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 979
BstDEI CTNAG 1 cut(s) 601
BstDSI CCRYGG 1 cut(s) 940
BstF5I GGATG 1 cut(s) 906
BstFNI CGCG 2 cut(s) 365, 367
BstHHI GCGC 1 cut(s) 367
BstMWI GCNNNNNNNGC 5 cut(s) 356, 362, 500, 733, 923
BstNSI RCATGY 2 cut(s) 898, 934
BstSFI CTRYAG 1 cut(s) 531
BstSLI GKGCMC 1 cut(s) 853
BstUI CGCG 2 cut(s) 365, 367
BstV1I GCAGC 1 cut(s) 343
BsuRI GGCC 4 cut(s) 226, 444, 594, 884
BtgI CCRYGG 1 cut(s) 940
BtrI CACGTC 1 cut(s) 673
BtsCI GGATG 1 cut(s) 906
BtsIMutI CAGTG 1 cut(s) 196
CciI TCATGA 1 cut(s) 768
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 442
Csp6I GTAC 5 cut(s) 10, 369, 384, 830, 980
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 8 cut(s) 94, 269, 490, 505, 769, 895, 931, 941
CviQI GTAC 5 cut(s) 10, 369, 384, 830, 980
DdeI CTNAG 1 cut(s) 601
DraI TTTAAA 2 cut(s) 255, 456
DraIII CACNNNGTG 2 cut(s) 212, 857
EaeI YGGCCR 1 cut(s) 882
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 287, 940
Eco147I AGGCCT 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 280
EcoT14I CCWWGG 2 cut(s) 287, 940
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 287, 940
FaeI CATG 8 cut(s) 97, 272, 493, 508, 772, 898, 934, 944
FatI CATG 8 cut(s) 93, 268, 489, 504, 768, 894, 930, 940
FauI CCCGC 1 cut(s) 487
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 1 cut(s) 893
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 1 cut(s) 914
GlaI GCGC 1 cut(s) 366
GluI GCNGC 1 cut(s) 357
HaeIII GGCC 4 cut(s) 226, 444, 594, 884
HhaI GCGC 1 cut(s) 367
Hin1II CATG 8 cut(s) 97, 272, 493, 508, 772, 898, 934, 944
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HinfI GANTC 4 cut(s) 411, 449, 539, 772
HphI GGTGA 4 cut(s) 114, 198, 662, 895
Hpy166II GTNNAC 6 cut(s) 10, 122, 705, 830, 867, 964
Hpy188I TCNGA 3 cut(s) 824, 838, 1018
Hpy188III TCNNGA 3 cut(s) 180, 408, 769
Hpy8I GTNNAC 6 cut(s) 10, 122, 705, 830, 867, 964
Hpy99I CGWCG 1 cut(s) 63
HpyAV CCTTC 3 cut(s) 641, 647, 658
HpyCH4III ACNGT 4 cut(s) 191, 391, 617, 628
HpyCH4IV ACGT 3 cut(s) 58, 524, 672
HpyCH4V TGCA 9 cut(s) 86, 145, 197, 215, 350, 380, 434, 676, 992
HpyF10VI GCNNNNNNNGC 5 cut(s) 356, 362, 500, 733, 923
HpyF3I CTNAG 1 cut(s) 601
HpySE526I ACGT 3 cut(s) 58, 524, 672
Hsp92II CATG 8 cut(s) 97, 272, 493, 508, 772, 898, 934, 944
HspAI GCGC 1 cut(s) 365
LguI GCTCTTC 1 cut(s) 70
LpnPI CCDG 9 cut(s) 22, 96, 193, 393, 713, 863, 875, 968, 978
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 1 cut(s) 184
MaeI CTAG 1 cut(s) 914
MaeII ACGT 3 cut(s) 58, 524, 672
MaeIII GTNAC 1 cut(s) 540
MboII GAAGA 2 cut(s) 57, 230
MhlI GDGCHC 2 cut(s) 282, 853
MlsI TGGCCA 1 cut(s) 884
MluCI AATT 8 cut(s) 46, 158, 165, 250, 294, 641, 680, 788
MluNI TGGCCA 1 cut(s) 884
MlyI GAGTC 2 cut(s) 405, 548
MmeI TCCRAC 2 cut(s) 559, 802
MnlI CCTC 8 cut(s) 23, 333, 409, 565, 610, 650, 786, 885
Mox20I TGGCCA 1 cut(s) 884
MroXI GAANNNNTTC 1 cut(s) 801
MscI TGGCCA 1 cut(s) 884
MseI TTAA 5 cut(s) 30, 54, 254, 455, 636
MslI CAYNNNNRTG 1 cut(s) 721
Msp20I TGGCCA 1 cut(s) 884
MspCI CTTAAG 2 cut(s) 29, 635
Mva1269I GAATGC 1 cut(s) 434
MvnI CGCG 2 cut(s) 365, 367
MwoI GCNNNNNNNGC 5 cut(s) 356, 362, 500, 733, 923
NcoI CCATGG 1 cut(s) 940
NlaIII CATG 8 cut(s) 97, 272, 493, 508, 772, 898, 934, 944
NmuCI GTSAC 1 cut(s) 540
NspI RCATGY 2 cut(s) 898, 934
PagI TCATGA 1 cut(s) 768
PceI AGGCCT 1 cut(s) 226
PciI ACATGT 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 70
PctI GAATGC 1 cut(s) 434
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 2 cut(s) 449, 772
Pfl23II CGTACG 1 cut(s) 368
PkrI GCNGC 1 cut(s) 358
PleI GAGTC 2 cut(s) 405, 547
PpsI GAGTC 2 cut(s) 405, 547
PscI ACATGT 1 cut(s) 930
Psp124BI GAGCTC 1 cut(s) 282
PspLI CGTACG 1 cut(s) 368
PspPI GGNCC 1 cut(s) 442
RsaI GTAC 5 cut(s) 11, 370, 385, 831, 981
RsaNI GTAC 5 cut(s) 10, 369, 384, 830, 980
RseI CAYNNNNRTG 1 cut(s) 721
SacI GAGCTC 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 5 cut(s) 30, 54, 254, 455, 636
SatI GCNGC 1 cut(s) 357
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 2 cut(s) 405, 548
SduI GDGCHC 2 cut(s) 282, 853
SfaNI GCATC 1 cut(s) 184
SfcI CTRYAG 1 cut(s) 531
SmiI ATTTAAAT 1 cut(s) 255
SmiMI CAYNNNNRTG 1 cut(s) 721
SmlI CTYRAG 2 cut(s) 29, 635
SmoI CTYRAG 2 cut(s) 29, 635
Sse9I AATT 8 cut(s) 46, 158, 165, 250, 294, 641, 680, 788
SseBI AGGCCT 1 cut(s) 226
SsiI CCGC 1 cut(s) 494
SspMI CTAG 1 cut(s) 914
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 1 cut(s) 226
StyI CCWWGG 2 cut(s) 287, 940
SwaI ATTTAAAT 1 cut(s) 255
TaaI ACNGT 4 cut(s) 191, 391, 617, 628
TaiI ACGT 3 cut(s) 61, 527, 675
TaqI TCGA 1 cut(s) 548
TasI AATT 8 cut(s) 46, 158, 165, 250, 294, 641, 680, 788
TatI WGTACW 3 cut(s) 383, 829, 979
TfiI GAWTC 2 cut(s) 449, 772
Tru1I TTAA 5 cut(s) 30, 54, 254, 455, 636
Tru9I TTAA 5 cut(s) 30, 54, 254, 455, 636
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 540
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 1 cut(s) 540
TspDTI ATGAA 4 cut(s) 110, 158, 757, 810
TspRI CASTG 1 cut(s) 196
Vha464I CTTAAG 2 cut(s) 29, 635
XapI RAATTY 2 cut(s) 165, 641
XceI RCATGY 2 cut(s) 898, 934
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.