RLG00000011585

Vacuolar membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002838
Physical Location & Seq
Forward (+)
10713761 .. 10716550
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011585

Sequence Viewer

Length: 1143 bp
ATGCAGGGGCAGCAAAACGAAGAAGTATCCAATAATTTGGATATCTATGAAAAGTATAAGCAGCCATTTGCAGTAACATATCTTGGAGCTTCTCTTATGGTAGTGTACCTCCCAATAGCTTTCCTTAAGGACTGGTTGTGTAATTTACTTAAACGTCGCTCTTCTAAAAGTAGCAAAAATGCAGAAAGCGTGAATGAGTATTCTGCTGGATTTAGTTCACCACTAAAACACAATGGAGTGCAGAAAGATTTTGAATTGGAAATTCATACCTTGTCCAGAAAAGACAGTGATGCAGACCTTTCACCCTGTGCAGAAGAAAGGCCTTTGGTTTCTAAACTCAAAGACAATTTAAATGTGCTGAAACATGACAGAGAGCTCACACCAAGGGAAATTGCTACTTATGGATTTTACCTTGCCCCTCTTTGGTTTTTTACAGAGTATTTTGCAAATGCTGCTCTCGCGCGTACGAGTGTTGCAAGTACAACAGTCTTATCCTCAACTTCAGGACTCTTTACACTCTTTATTGGTGCATTCTTGGGCCAAGATTCTTTAAATATAGCAAAAGTGGTGGCTGTCTTTGTCAGCATGGCGGGTGTAGCCATGACAACTATGGGAAAAACGTGGGCTACAGATGAGTCACAATCGAGTGCCTCCAATGGGAAACACTCTCTCGTTGGAGATCTTTTCGGCCTTTCCTCAGCTATGTCATACGGTCTATTTACAGTCCTTCTTAAGAAATTTGGTGGTGAAGGAGGAGAGAAGGCTGACGTGCAAAAATTGTTTGGGTATATTGGATTGTTTACACTTGTAGCATTATGGTGGCTGGTTTTGCCATTGACAGCAATGGGCATTGAACCAAAGTTCATGATTCCTCACTCTGCTAAATTAGATGAACTTGTTCTTGCCAATGGCTTTGTTGGAAGTGTACTTTCTGACTACTTTTGGGCACTATGTGTTGTGTGGACAACTCCACTGGTGGCCACTCTGGGCATGTCACTCACCATCCCCCTAGCTATGGTGGCTGACATGTTTATCCATGGTCGTCATTATTCGGCAGTTTACATTCTTGGCTCTGTACAGGTATTTGCAGGATTTGTAATAGCTAATCTTTCAGATTGGTTCTCAAAGAAGTTGGGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

381

Amino Acids

41.7

Weight (kDa)

6.39

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 126 - 203 1.8e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 461, 463
AciI CCGC 1 cut(s) 590
AcoI YGGCCR 1 cut(s) 978
AcsI RAATTY 2 cut(s) 261, 737
AcuI CTGAAG 1 cut(s) 486
AdeI CACNNNGTG 2 cut(s) 308, 953
AfaI GTAC 5 cut(s) 107, 466, 481, 927, 1077
AfiI CCNNNNNNNGG 3 cut(s) 423, 657, 1015
AflII CTTAAG 2 cut(s) 125, 731
AflIII ACRYGT 1 cut(s) 1026
AgsI TTSAA 2 cut(s) 254, 854
AjiI CACGTC 1 cut(s) 769
AluBI AGCT 6 cut(s) 89, 119, 376, 701, 1013, 1103
AluI AGCT 6 cut(s) 89, 119, 376, 701, 1013, 1103
Alw21I GWGCWC 1 cut(s) 378
AoxI GGCC 4 cut(s) 320, 538, 688, 978
ApeKI GCWGC 3 cut(s) 10, 61, 452
ApoI RAATTY 2 cut(s) 261, 737
Asp700I GAANNNNTTC 1 cut(s) 897
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 538
AsuHPI GGTGA 4 cut(s) 210, 294, 758, 991
BaeGI GKGCMC 1 cut(s) 949
BalI TGGCCA 1 cut(s) 980
BanII GRGCYC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 378
BbvCI CCTCAGC 1 cut(s) 697
BbvI GCAGC 3 cut(s) 22, 73, 439
BccI CCATC 1 cut(s) 1010
BciVI GTATCC 1 cut(s) 37
BfaI CTAG 1 cut(s) 1010
BfmI CTRYAG 1 cut(s) 627
BfrI CTTAAG 2 cut(s) 125, 731
BfuI GTATCC 1 cut(s) 37
BglII AGATCT 1 cut(s) 679
BisI GCNGC 3 cut(s) 11, 62, 453
BlsI GCNGC 3 cut(s) 12, 63, 454
BmgBI CACGTC 1 cut(s) 769
BmgT120I GGNCC 1 cut(s) 538
BmsI GCATC 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 697
BsaJI CCNNGG 2 cut(s) 383, 1036
Bsc4I CCNNNNNNNGG 3 cut(s) 423, 657, 1015
Bse1I ACTGG 2 cut(s) 137, 978
Bse3DI GCAATG 1 cut(s) 849
BseDI CCNNGG 2 cut(s) 383, 1036
BseGI GGATG 1 cut(s) 1002
BseLI CCNNNNNNNGG 3 cut(s) 423, 657, 1015
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 1 cut(s) 711
BseNI ACTGG 2 cut(s) 137, 978
BseRI GAGGAG 1 cut(s) 768
BseSI GKGCMC 1 cut(s) 949
BseXI GCAGC 3 cut(s) 22, 73, 439
BsgI GTGCAG 2 cut(s) 260, 330
Bsh1236I CGCG 2 cut(s) 461, 463
BshFI GGCC 4 cut(s) 322, 540, 690, 980
BsiHKAI GWGCWC 1 cut(s) 378
BsiWI CGTACG 1 cut(s) 464
BslI CCNNNNNNNGG 3 cut(s) 423, 657, 1015
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 4 cut(s) 322, 540, 690, 980
Bsp1286I GDGCHC 2 cut(s) 378, 949
Bsp1407I TGTACA 1 cut(s) 1075
Bsp143I GATC 1 cut(s) 679
Bsp19I CCATGG 1 cut(s) 1036
BspACI CCGC 1 cut(s) 590
BspANI GGCC 4 cut(s) 322, 540, 690, 980
BspCNI CTCAG 1 cut(s) 710
BspFNI CGCG 2 cut(s) 461, 463
BspHI TCATGA 1 cut(s) 864
BspQI GCTCTTC 1 cut(s) 166
BspTI CTTAAG 2 cut(s) 125, 731
BsrDI GCAATG 1 cut(s) 849
BsrGI TGTACA 1 cut(s) 1075
BsrI ACTGG 2 cut(s) 137, 978
BssECI CCNNGG 2 cut(s) 383, 1036
BssMI GATC 1 cut(s) 679
BssT1I CCWWGG 2 cut(s) 383, 1036
Bst4CI ACNGT 4 cut(s) 287, 487, 713, 724
Bst6I CTCTTC 1 cut(s) 166
BstAFI CTTAAG 2 cut(s) 125, 731
BstAPI GCANNNNNTGC 1 cut(s) 452
BstAUI TGTACA 1 cut(s) 1075
BstDEI CTNAG 1 cut(s) 697
BstDSI CCRYGG 1 cut(s) 1036
BstF5I GGATG 1 cut(s) 1002
BstFNI CGCG 2 cut(s) 461, 463
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 1 cut(s) 682
BstMBI GATC 1 cut(s) 679
BstMWI GCNNNNNNNGC 6 cut(s) 10, 452, 458, 596, 829, 1019
BstNSI RCATGY 2 cut(s) 994, 1030
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 1 cut(s) 949
BstUI CGCG 2 cut(s) 461, 463
BstV1I GCAGC 3 cut(s) 22, 73, 439
BstX2I RGATCY 1 cut(s) 679
BstXI CCANNNNNNTGG 1 cut(s) 37
BstYI RGATCY 1 cut(s) 679
BsuI GTATCC 1 cut(s) 37
BsuRI GGCC 4 cut(s) 322, 540, 690, 980
BtgI CCRYGG 1 cut(s) 1036
BtrI CACGTC 1 cut(s) 769
BtsCI GGATG 1 cut(s) 1002
BtsIMutI CAGTG 2 cut(s) 292, 971
CciI TCATGA 1 cut(s) 864
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 538
Csp6I GTAC 5 cut(s) 106, 465, 480, 926, 1076
CspCI CAANNNNNGTGG 2 cut(s) 549, 584
CviAII CATG 7 cut(s) 365, 586, 601, 865, 991, 1027, 1037
CviQI GTAC 5 cut(s) 106, 465, 480, 926, 1076
DdeI CTNAG 1 cut(s) 697
DpnI GATC 1 cut(s) 681
DpnII GATC 1 cut(s) 679
DraI TTTAAA 2 cut(s) 351, 552
DraIII CACNNNGTG 2 cut(s) 308, 953
EaeI YGGCCR 1 cut(s) 978
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 2 cut(s) 383, 1036
Eco147I AGGCCT 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 378
Eco32I GATATC 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 486
EcoICRI GAGCTC 1 cut(s) 376
EcoRV GATATC 1 cut(s) 43
EcoT14I CCWWGG 2 cut(s) 383, 1036
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 2 cut(s) 383, 1036
FaeI CATG 7 cut(s) 368, 589, 604, 868, 994, 1030, 1040
FatI CATG 7 cut(s) 364, 585, 600, 864, 990, 1026, 1036
FauI CCCGC 1 cut(s) 583
Fnu4HI GCNGC 3 cut(s) 11, 62, 453
FokI GGATG 1 cut(s) 989
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 3 cut(s) 11, 62, 453
FspBI CTAG 1 cut(s) 1010
GlaI GCGC 1 cut(s) 462
GluI GCNGC 3 cut(s) 11, 62, 453
HaeIII GGCC 4 cut(s) 322, 540, 690, 980
HhaI GCGC 1 cut(s) 463
Hin1II CATG 7 cut(s) 368, 589, 604, 868, 994, 1030, 1040
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HinfI GANTC 4 cut(s) 507, 545, 635, 868
HphI GGTGA 4 cut(s) 210, 294, 758, 991
Hpy166II GTNNAC 6 cut(s) 106, 218, 801, 926, 963, 1060
Hpy188I TCNGA 2 cut(s) 934, 1114
Hpy188III TCNNGA 3 cut(s) 276, 504, 865
Hpy8I GTNNAC 6 cut(s) 106, 218, 801, 926, 963, 1060
Hpy99I CGWCG 1 cut(s) 159
HpyAV CCTTC 3 cut(s) 737, 743, 754
HpyCH4III ACNGT 4 cut(s) 287, 487, 713, 724
HpyCH4IV ACGT 3 cut(s) 154, 620, 768
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 452, 458, 596, 829, 1019
HpyF3I CTNAG 1 cut(s) 697
HpySE526I ACGT 3 cut(s) 154, 620, 768
Hsp92II CATG 7 cut(s) 368, 589, 604, 868, 994, 1030, 1040
HspAI GCGC 1 cut(s) 461
Kzo9I GATC 1 cut(s) 679
LguI GCTCTTC 1 cut(s) 166
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 3 cut(s) 22, 73, 439
LweI GCATC 1 cut(s) 280
MaeI CTAG 1 cut(s) 1010
MaeII ACGT 3 cut(s) 154, 620, 768
MaeIII GTNAC 3 cut(s) 73, 636, 993
MalI GATC 1 cut(s) 681
MboI GATC 1 cut(s) 679
MboII GAAGA 3 cut(s) 32, 153, 326
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 2 cut(s) 378, 949
MlsI TGGCCA 1 cut(s) 980
MluCI AATT 9 cut(s) 34, 142, 254, 261, 346, 390, 737, 776, 884
MluNI TGGCCA 1 cut(s) 980
MlyI GAGTC 2 cut(s) 501, 644
MmeI TCCRAC 2 cut(s) 655, 898
MnlI CCTC 7 cut(s) 119, 429, 505, 661, 706, 746, 882
Mox20I TGGCCA 1 cut(s) 980
MroXI GAANNNNTTC 1 cut(s) 897
MscI TGGCCA 1 cut(s) 980
MseI TTAA 5 cut(s) 126, 150, 350, 551, 732
MslI CAYNNNNRTG 1 cut(s) 817
Msp20I TGGCCA 1 cut(s) 980
MspCI CTTAAG 2 cut(s) 125, 731
Mva1269I GAATGC 1 cut(s) 530
MvnI CGCG 2 cut(s) 461, 463
MwoI GCNNNNNNNGC 6 cut(s) 10, 452, 458, 596, 829, 1019
NcoI CCATGG 1 cut(s) 1036
NdeII GATC 1 cut(s) 679
NlaIII CATG 7 cut(s) 368, 589, 604, 868, 994, 1030, 1040
NmuCI GTSAC 2 cut(s) 636, 993
NspI RCATGY 2 cut(s) 994, 1030
PagI TCATGA 1 cut(s) 864
PceI AGGCCT 1 cut(s) 322
PciI ACATGT 1 cut(s) 1026
PciSI GCTCTTC 1 cut(s) 166
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 897
PfeI GAWTC 2 cut(s) 545, 868
Pfl23II CGTACG 1 cut(s) 464
PkrI GCNGC 3 cut(s) 12, 63, 454
PleI GAGTC 2 cut(s) 501, 643
PpsI GAGTC 2 cut(s) 501, 643
PscI ACATGT 1 cut(s) 1026
Psp124BI GAGCTC 1 cut(s) 378
PspLI CGTACG 1 cut(s) 464
PspPI GGNCC 1 cut(s) 538
PsuI RGATCY 1 cut(s) 679
RsaI GTAC 5 cut(s) 107, 466, 481, 927, 1077
RsaNI GTAC 5 cut(s) 106, 465, 480, 926, 1076
RseI CAYNNNNRTG 1 cut(s) 817
SacI GAGCTC 1 cut(s) 378
SapI GCTCTTC 1 cut(s) 166
SaqAI TTAA 5 cut(s) 126, 150, 350, 551, 732
SatI GCNGC 3 cut(s) 11, 62, 453
Sau3AI GATC 1 cut(s) 679
Sau96I GGNCC 1 cut(s) 538
SchI GAGTC 2 cut(s) 501, 644
SduI GDGCHC 2 cut(s) 378, 949
SfaNI GCATC 1 cut(s) 280
SfcI CTRYAG 1 cut(s) 627
SmiI ATTTAAAT 1 cut(s) 351
SmiMI CAYNNNNRTG 1 cut(s) 817
SmlI CTYRAG 2 cut(s) 125, 731
SmoI CTYRAG 2 cut(s) 125, 731
Sse9I AATT 9 cut(s) 34, 142, 254, 261, 346, 390, 737, 776, 884
SseBI AGGCCT 1 cut(s) 322
SsiI CCGC 1 cut(s) 590
SspMI CTAG 1 cut(s) 1010
SstI GAGCTC 1 cut(s) 378
StuI AGGCCT 1 cut(s) 322
StyI CCWWGG 2 cut(s) 383, 1036
SwaI ATTTAAAT 1 cut(s) 351
TaaI ACNGT 4 cut(s) 287, 487, 713, 724
TaiI ACGT 3 cut(s) 157, 623, 771
TaqI TCGA 1 cut(s) 644
TasI AATT 9 cut(s) 34, 142, 254, 261, 346, 390, 737, 776, 884
TatI WGTACW 3 cut(s) 479, 925, 1075
TfiI GAWTC 2 cut(s) 545, 868
Tru1I TTAA 5 cut(s) 126, 150, 350, 551, 732
Tru9I TTAA 5 cut(s) 126, 150, 350, 551, 732
TscAI CASTG 2 cut(s) 292, 978
TseFI GTSAC 2 cut(s) 636, 993
TseI GCWGC 3 cut(s) 10, 61, 452
Tsp45I GTSAC 2 cut(s) 636, 993
TspDTI ATGAA 4 cut(s) 63, 254, 853, 906
TspRI CASTG 2 cut(s) 292, 978
Vha464I CTTAAG 2 cut(s) 125, 731
XapI RAATTY 2 cut(s) 261, 737
XceI RCATGY 2 cut(s) 994, 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmnI GAANNNNTTC 1 cut(s) 897
XspI CTAG 1 cut(s) 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.