AT5G15120

2-aminoethanethiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002785
Physical Location & Seq
Forward (+)
4898641 .. 4900734
2094 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G15120.1

Sequence Viewer

Length: 882 bp
ATGGGGTTTGAGATGAAACCAGAGAAAGAGGTTTTGGAATTGATTTCTAGTAAGAATCAATGCAAGTCTAATCCCAATTCTGTGAAGAAGAAGAACAAAAACAAGAATAAGAAGATGATGATGACGTGGCGACGGAAGAAGATTGATTCCCCGGCGGATGGGATCACGGCGGTGCGGCGGTTGTTTAATACTTGCAAGGAAGTGTTCTCTAATGGTGGTCCTGGTGTTATTCCTTCTGAAGATAAAATCCAACAGCTACGAGAGATTCTTGACGATATGAAACCAGAGGATGTCGGTTTAACTCCGACCATGCCGTATTTCCGACCAAACTCCGGAGTCGAAGCTCGGTCTTCGCCACCAATAACGTATCTGCATCTACACCAGTGTGATCAATTCTCGATTGGGATTTTCTGTTTGCCACCTTCTGGTGTTATTCCTCTTCATAACCATCCAGGGATGACAGTTTTTAGTAAGCTTCTCTTTGGTACAATGCACATCAAGTCATATGATTGGGTGGTTGATGCTCCAATGAGAGATTCAAAAACCAGGCTAGCGAAATTGAAGGTGGATTCAACATTCACCGCGCCATGTAACGCCTCGATTTTGTACCCGGAAGATGGCGGGAACATGCATCGGTTCACAGCTATAACAGCATGTGCGGTGCTAGACGTGCTAGGACCTCCTTACTGCAATCCAGAAGGCAGACATTGCACTTATTTCCTAGAATTCCCTTTAGACAAACTCTCATCAGAGGACGACGACGTTTTGAGCAGTGAAGAGGAGAAGGAAGGTTATGCATGGCTGCAAGAAAGGGATGATAATCCAGAAGATCATACTAACGTAGTTGGAGCGTTATACAGAGGCCCAAAGGTTGAAGATTGA

Protein Analysis

293

Amino Acids

33.01

Weight (kDa)

6.1

Isoelectric Point (pI)

52.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 89 - 291 2.2e-75 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 425
AccII CGCG 1 cut(s) 584
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 7 cut(s) 155, 170, 175, 178, 582, 621, 659
AclWI GGATC 1 cut(s) 170
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 2 cut(s) 487, 608
AfiI CCNNNNNNNGG 4 cut(s) 158, 332, 425, 617
AgsI TTSAA 4 cut(s) 540, 562, 573, 875
AjiI CACGTC 2 cut(s) 126, 670
AjnI CCWGG 3 cut(s) 220, 451, 545
AjuI GAANNNNNNNTTGG 4 cut(s) 17, 49, 520, 552
AleI CACNNNNGTG 2 cut(s) 170, 384
AluBI AGCT 4 cut(s) 256, 344, 475, 644
AluI AGCT 4 cut(s) 256, 344, 475, 644
AlwI GGATC 1 cut(s) 170
Aor13HI TCCGGA 1 cut(s) 332
AoxI GGCC 1 cut(s) 862
ApeKI GCWGC 1 cut(s) 802
ApoI RAATTY 1 cut(s) 725
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 3 cut(s) 218, 677, 863
AsuC2I CCSGG 2 cut(s) 152, 611
AsuHPI GGTGA 1 cut(s) 571
AsuNHI GCTAGC 1 cut(s) 550
AvaII GGWCC 2 cut(s) 218, 677
BbsI GAAGAC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 789
BccI CCATC 3 cut(s) 152, 456, 611
BceAI ACGGC 2 cut(s) 183, 298
BciT130I CCWGG 3 cut(s) 222, 453, 547
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 2 cut(s) 152, 611
BfaI CTAG 5 cut(s) 48, 551, 665, 674, 722
BisI GCNGC 2 cut(s) 176, 803
BlsI GCNGC 2 cut(s) 177, 804
Bme1390I CCNGG 5 cut(s) 152, 222, 453, 547, 611
Bme18I GGWCC 2 cut(s) 218, 677
BmgBI CACGTC 2 cut(s) 126, 670
BmgT120I GGNCC 3 cut(s) 218, 677, 863
BmrFI CCNGG 5 cut(s) 152, 222, 453, 547, 611
BmsI GCATC 3 cut(s) 382, 511, 640
BmtI GCTAGC 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 342
BpuMI CCSGG 2 cut(s) 152, 611
BsaBI GATNNNNATC 1 cut(s) 819
BsaJI CCNNGG 2 cut(s) 150, 452
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 332, 425, 617
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 706
Bse8I GATNNNNATC 1 cut(s) 819
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 3 cut(s) 222, 453, 547
BseDI CCNNGG 2 cut(s) 150, 452
BseGI GGATG 5 cut(s) 163, 295, 448, 462, 820
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 4 cut(s) 158, 332, 425, 617
BseMI GCAATG 1 cut(s) 706
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 1 cut(s) 794
BseXI GCAGC 1 cut(s) 789
Bsh1236I CGCG 1 cut(s) 584
BshFI GGCC 1 cut(s) 864
BsiSI CCGG 3 cut(s) 152, 333, 611
BslI CCNNNNNNNGG 4 cut(s) 158, 332, 425, 617
BsnI GGCC 1 cut(s) 864
Bsp13I TCCGGA 1 cut(s) 332
Bsp143I GATC 3 cut(s) 162, 388, 829
BspACI CCGC 7 cut(s) 155, 170, 175, 178, 582, 621, 659
BspANI GGCC 1 cut(s) 864
BspEI TCCGGA 1 cut(s) 332
BspFNI CGCG 1 cut(s) 584
BspOI GCTAGC 1 cut(s) 554
BspPI GGATC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 706
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 2 cut(s) 150, 452
BssMI GATC 3 cut(s) 162, 388, 829
Bst2UI CCWGG 3 cut(s) 222, 453, 547
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 2 cut(s) 444, 771
BstAPI GCANNNNNTGC 1 cut(s) 708
BstC8I GCNNGC 1 cut(s) 552
BstF5I GGATG 5 cut(s) 163, 295, 448, 462, 820
BstFNI CGCG 1 cut(s) 584
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 3 cut(s) 165, 391, 832
BstMBI GATC 3 cut(s) 162, 388, 829
BstMWI GCNNNNNNNGC 3 cut(s) 650, 670, 708
BstNI CCWGG 3 cut(s) 222, 453, 547
BstNSI RCATGY 2 cut(s) 631, 657
BstSCI CCNGG 5 cut(s) 150, 220, 451, 545, 609
BstUI CGCG 1 cut(s) 584
BstV1I GCAGC 1 cut(s) 789
BstV2I GAAGAC 1 cut(s) 342
BsuRI GGCC 1 cut(s) 864
BtrI CACGTC 2 cut(s) 126, 670
BtsCI GGATG 5 cut(s) 163, 295, 448, 462, 820
BtsI GCAGTG 1 cut(s) 778
BtsIMutI CAGTG 2 cut(s) 389, 778
Cac8I GCNNGC 1 cut(s) 552
CfoI GCGC 1 cut(s) 586
Cfr13I GGNCC 3 cut(s) 218, 677, 863
Csp6I GTAC 2 cut(s) 486, 607
CviAII CATG 5 cut(s) 310, 588, 628, 654, 798
CviJI RGCY 7 cut(s) 256, 344, 475, 550, 644, 802, 864
CviKI_1 RGCY 7 cut(s) 256, 344, 475, 550, 644, 802, 864
CviQI GTAC 2 cut(s) 486, 607
DpnI GATC 3 cut(s) 164, 390, 831
DpnII GATC 3 cut(s) 162, 388, 829
Eam1104I CTCTTC 2 cut(s) 444, 771
EarI CTCTTC 2 cut(s) 444, 771
EciI GGCGGA 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 218, 677
Eco57I CTGAAG 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 677
EcoRI GAATTC 1 cut(s) 725
EcoRII CCWGG 3 cut(s) 220, 451, 545
EcoT22I ATGCAT 2 cut(s) 633, 799
FaeI CATG 5 cut(s) 313, 591, 631, 657, 801
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FatI CATG 5 cut(s) 309, 587, 627, 653, 797
FauI CCCGC 1 cut(s) 614
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 176, 803
FokI GGATG 5 cut(s) 170, 302, 435, 469, 827
Fsp4HI GCNGC 2 cut(s) 176, 803
FspBI CTAG 5 cut(s) 48, 551, 665, 674, 722
GlaI GCGC 1 cut(s) 585
GluI GCNGC 2 cut(s) 176, 803
HaeIII GGCC 1 cut(s) 864
HapII CCGG 3 cut(s) 152, 333, 611
HhaI GCGC 1 cut(s) 586
Hin1II CATG 5 cut(s) 313, 591, 631, 657, 801
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 6 cut(s) 55, 146, 265, 336, 536, 569
HpaII CCGG 3 cut(s) 152, 333, 611
HphI GGTGA 1 cut(s) 571
Hpy166II GTNNAC 1 cut(s) 639
Hpy188I TCNGA 4 cut(s) 238, 306, 323, 751
Hpy188III TCNNGA 5 cut(s) 269, 333, 397, 695, 824
Hpy8I GTNNAC 1 cut(s) 639
Hpy99I CGWCG 3 cut(s) 135, 761, 764
HpyAV CCTTC 6 cut(s) 243, 432, 556, 692, 778, 782
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4IV ACGT 5 cut(s) 125, 365, 669, 762, 840
HpyCH4V TGCA 9 cut(s) 63, 195, 373, 493, 631, 690, 711, 797, 805
HpyF10VI GCNNNNNNNGC 3 cut(s) 650, 670, 708
HpySE526I ACGT 5 cut(s) 125, 365, 669, 762, 840
Hsp92II CATG 5 cut(s) 313, 591, 631, 657, 801
HspAI GCGC 1 cut(s) 584
Kpn2I TCCGGA 1 cut(s) 332
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 3 cut(s) 162, 388, 829
LmnI GCTCC 2 cut(s) 529, 848
Lsp1109I GCAGC 1 cut(s) 789
LweI GCATC 3 cut(s) 382, 511, 640
MaeI CTAG 5 cut(s) 48, 551, 665, 674, 722
MaeII ACGT 5 cut(s) 125, 365, 669, 762, 840
MaeIII GTNAC 1 cut(s) 590
MalI GATC 3 cut(s) 164, 390, 831
MboI GATC 3 cut(s) 162, 388, 829
MluCI AATT 5 cut(s) 38, 76, 392, 557, 725
MlyI GAGTC 1 cut(s) 345
MmeI TCCRAC 4 cut(s) 274, 329, 346, 826
MnlI CCTC 8 cut(s) 22, 280, 447, 607, 690, 745, 772, 854
Mph1103I ATGCAT 2 cut(s) 633, 799
MroI TCCGGA 1 cut(s) 332
MseI TTAA 2 cut(s) 186, 299
MslI CAYNNNNRTG 2 cut(s) 170, 384
MspI CCGG 3 cut(s) 152, 333, 611
MspR9I CCNGG 5 cut(s) 152, 222, 453, 547, 611
MvaI CCWGG 3 cut(s) 222, 453, 547
MvnI CGCG 1 cut(s) 584
MwoI GCNNNNNNNGC 3 cut(s) 650, 670, 708
NciI CCSGG 2 cut(s) 152, 611
NdeI CATATG 1 cut(s) 505
NdeII GATC 3 cut(s) 162, 388, 829
NheI GCTAGC 1 cut(s) 550
NlaIII CATG 5 cut(s) 313, 591, 631, 657, 801
NsiI ATGCAT 2 cut(s) 633, 799
NspI RCATGY 2 cut(s) 631, 657
OliI CACNNNNGTG 2 cut(s) 170, 384
PfeI GAWTC 5 cut(s) 55, 146, 265, 536, 569
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 2 cut(s) 177, 804
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
PpuMI RGGWCCY 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 677
Psp6I CCWGG 3 cut(s) 220, 451, 545
PspGI CCWGG 3 cut(s) 220, 451, 545
PspPI GGNCC 3 cut(s) 218, 677, 863
PspPPI RGGWCCY 1 cut(s) 677
RsaI GTAC 2 cut(s) 487, 608
RsaNI GTAC 2 cut(s) 486, 607
RseI CAYNNNNRTG 2 cut(s) 170, 384
SaqAI TTAA 2 cut(s) 186, 299
SatI GCNGC 2 cut(s) 176, 803
Sau3AI GATC 3 cut(s) 162, 388, 829
Sau96I GGNCC 3 cut(s) 218, 677, 863
SchI GAGTC 1 cut(s) 345
ScrFI CCNGG 5 cut(s) 152, 222, 453, 547, 611
SfaNI GCATC 3 cut(s) 382, 511, 640
SinI GGWCC 2 cut(s) 218, 677
SmiMI CAYNNNNRTG 2 cut(s) 170, 384
Sse9I AATT 5 cut(s) 38, 76, 392, 557, 725
SsiI CCGC 7 cut(s) 155, 170, 175, 178, 582, 621, 659
SspMI CTAG 5 cut(s) 48, 551, 665, 674, 722
StyD4I CCNGG 5 cut(s) 150, 220, 451, 545, 609
TaaI ACNGT 1 cut(s) 463
TaiI ACGT 5 cut(s) 128, 368, 672, 765, 843
TaqI TCGA 3 cut(s) 339, 398, 599
TaqII GACCGA 1 cut(s) 336
TasI AATT 5 cut(s) 38, 76, 392, 557, 725
TauI GCSGC 1 cut(s) 178
TfiI GAWTC 5 cut(s) 55, 146, 265, 536, 569
Tru1I TTAA 2 cut(s) 186, 299
Tru9I TTAA 2 cut(s) 186, 299
TscAI CASTG 2 cut(s) 389, 778
TseI GCWGC 1 cut(s) 802
TspDTI ATGAA 3 cut(s) 29, 293, 431
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 2 cut(s) 389, 778
Van91I CCANNNNNTGG 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 218, 677
XapI RAATTY 1 cut(s) 725
XceI RCATGY 2 cut(s) 631, 657
XspI CTAG 5 cut(s) 48, 551, 665, 674, 722
Zsp2I ATGCAT 2 cut(s) 633, 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.