MD14G1006600.v1.1

2-aminoethanethiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002785
Physical Location & Seq
Forward (+)
730544 .. 733643
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1006600.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGAGACTGCGAGGGTGGTGGAGCAGGGAAGACATCAGATGCCGCCGTCGAAGGTTCCAACTCCGCTGAAACAGCTCTTTCGTGCGTGCAGACAAGTTTTCAAAGGCCCCGGAAACGTTCCTTTGCCTCACCATGTGCACAAGCTGTGTCGCATCCTCGATAATATGAGACCAGAAGATGTTGGGCTAAGTAGGGATCTGCAGTTCTTCAAGCGTAAAGCTGTTGTGAAAGGGACTCCTAGGGTCACGTATACAACCATATATGAGTGCAACAAATTTTCGTTGTGCTGCTTTTTTATTCCGGCAAATGGTGTTATCCCACTCCATAACCATCCAGATATGACTGTTTTCAGTAAGCTTCTATTAGGCGAGATGCATATTAAATCATACGATTGGGTTGATCCCGTAAATTCAGATGGCTCCACGCCAGCGCCTCAATTGAGACTGGCCGAGTTGAAAGCTAATGATGTCTTCACAGCCTCATGCAAAGCATCAGTACTTTATCCAACAGAAGGTGGCAACATCCATGCCTTCACAGCCAAAACACCATGTGCAGTGCTCGATGTGATGGGACCACCCTATTCTAAAGAAGATGGCCGGGATTGCACGTATTACAGAGACCATCCCTATGCTGCTTGTTCAAATGGAGGAGCAGCGGAAACTGAAGAGAAACACGATAGTTACTGGTGGTTGGAAGAGATTGAAATGCCTGAGTACGCAAAAATGGATAGAATCGAGTACTTGGGTCCACAAGTTACCAAGAGGAGGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

258

Amino Acids

29.1

Weight (kDa)

8.03

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 52 - 252 4.3e-72 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 251
AciI CCGC 3 cut(s) 44, 65, 656
AclI AACGTT 1 cut(s) 117
AclWI GGATC 2 cut(s) 204, 395
AcoI YGGCCR 2 cut(s) 447, 595
AcsI RAATTY 2 cut(s) 275, 409
AcuI CTGAAG 1 cut(s) 684
AdeI CACNNNGTG 2 cut(s) 136, 551
AfaI GTAC 3 cut(s) 498, 716, 740
AfiI CCNNNNNNNGG 3 cut(s) 308, 512, 765
AgsI TTSAA 5 cut(s) 103, 211, 457, 642, 704
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
AluBI AGCT 5 cut(s) 76, 145, 221, 358, 461
AluI AGCT 5 cut(s) 76, 145, 221, 358, 461
Alw21I GWGCWC 2 cut(s) 141, 561
Alw26I GTCTC 3 cut(s) 163, 436, 612
Alw44I GTGCAC 1 cut(s) 137
AlwI GGATC 2 cut(s) 204, 395
AoxI GGCC 3 cut(s) 106, 447, 595
ApaLI GTGCAC 1 cut(s) 137
ApeKI GCWGC 3 cut(s) 288, 632, 653
ApoI RAATTY 2 cut(s) 275, 409
AspA2I CCTAGG 1 cut(s) 239
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 3 cut(s) 107, 572, 746
AsuC2I CCSGG 2 cut(s) 111, 599
AsuHPI GGTGA 1 cut(s) 122
AvaII GGWCC 2 cut(s) 572, 746
AvrII CCTAGG 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 141
BbsI GAAGAC 2 cut(s) 37, 463
Bbv12I GWGCWC 2 cut(s) 141, 561
BbvI GCAGC 3 cut(s) 275, 619, 665
BccI CCATC 5 cut(s) 339, 410, 562, 587, 630
BceAI ACGGC 1 cut(s) 31
BcnI CCSGG 2 cut(s) 111, 599
BcoDI GTCTC 3 cut(s) 163, 436, 612
BfaI CTAG 1 cut(s) 240
BfmI CTRYAG 1 cut(s) 200
BfoI RGCGCY 1 cut(s) 434
BisI GCNGC 4 cut(s) 44, 289, 633, 654
BlnI CCTAGG 1 cut(s) 239
BlsI GCNGC 4 cut(s) 45, 290, 634, 655
BmcAI AGTACT 2 cut(s) 498, 740
Bme1390I CCNGG 2 cut(s) 111, 599
Bme18I GGWCC 2 cut(s) 572, 746
BmgT120I GGNCC 3 cut(s) 107, 572, 746
BmiI GGNNCC 5 cut(s) 57, 109, 421, 573, 747
BmrFI CCNGG 2 cut(s) 111, 599
BmsI GCATC 4 cut(s) 30, 162, 363, 500
BpiI GAAGAC 2 cut(s) 37, 463
BpuMI CCSGG 2 cut(s) 111, 599
BsaAI YACGTR 2 cut(s) 249, 609
BsaI GGTCTC 2 cut(s) 163, 612
BsaJI CCNNGG 2 cut(s) 109, 239
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 512, 765
Bse1I ACTGG 2 cut(s) 450, 689
BseDI CCNNGG 2 cut(s) 109, 239
BseGI GGATG 4 cut(s) 153, 331, 522, 622
BseLI CCNNNNNNNGG 3 cut(s) 308, 512, 765
BseMII CTCAG 1 cut(s) 702
BseNI ACTGG 2 cut(s) 450, 689
BseRI GAGGAG 1 cut(s) 663
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 3 cut(s) 275, 619, 665
BsgI GTGCAG 2 cut(s) 109, 573
BshFI GGCC 3 cut(s) 108, 449, 597
BsiHKAI GWGCWC 2 cut(s) 141, 561
BsiSI CCGG 3 cut(s) 111, 302, 598
BslFI GGGAC 2 cut(s) 247, 585
BslI CCNNNNNNNGG 3 cut(s) 308, 512, 765
BsmAI GTCTC 3 cut(s) 163, 436, 612
BsmFI GGGAC 2 cut(s) 247, 585
BsnI GGCC 3 cut(s) 108, 449, 597
Bso31I GGTCTC 2 cut(s) 163, 612
Bsp1286I GDGCHC 2 cut(s) 141, 561
Bsp143I GATC 2 cut(s) 196, 400
BspACI CCGC 3 cut(s) 44, 65, 656
BspANI GGCC 3 cut(s) 108, 449, 597
BspCNI CTCAG 1 cut(s) 703
BspLI GGNNCC 5 cut(s) 57, 109, 421, 573, 747
BspMAI CTGCAG 1 cut(s) 204
BspPI GGATC 2 cut(s) 204, 395
BspTNI GGTCTC 2 cut(s) 163, 612
BsrI ACTGG 2 cut(s) 450, 689
BssECI CCNNGG 2 cut(s) 109, 239
BssMI GATC 2 cut(s) 196, 400
BssNAI GTATAC 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 239
Bst1107I GTATAC 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 2 cut(s) 660, 690
BstBAI YACGTR 2 cut(s) 249, 609
BstC8I GCNNGC 2 cut(s) 88, 429
BstDEI CTNAG 2 cut(s) 188, 711
BstF5I GGATG 4 cut(s) 153, 331, 522, 622
BstH2I RGCGCY 1 cut(s) 434
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 2 cut(s) 199, 403
BstMAI GTCTC 3 cut(s) 163, 436, 612
BstMBI GATC 2 cut(s) 196, 400
BstMWI GCNNNNNNNGC 3 cut(s) 73, 536, 603
BstSCI CCNGG 2 cut(s) 109, 597
BstSFI CTRYAG 1 cut(s) 200
BstSLI GKGCMC 1 cut(s) 141
BstV1I GCAGC 3 cut(s) 275, 619, 665
BstV2I GAAGAC 2 cut(s) 37, 463
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BstZ17I GTATAC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 108, 449, 597
BtsCI GGATG 4 cut(s) 153, 331, 522, 622
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 2 cut(s) 88, 429
CfoI GCGC 1 cut(s) 433
Cfr13I GGNCC 3 cut(s) 107, 572, 746
Csp6I GTAC 3 cut(s) 497, 715, 739
CviAII CATG 4 cut(s) 134, 483, 527, 549
CviQI GTAC 3 cut(s) 497, 715, 739
DdeI CTNAG 2 cut(s) 188, 711
DpnI GATC 2 cut(s) 198, 402
DpnII GATC 2 cut(s) 196, 400
DraIII CACNNNGTG 2 cut(s) 136, 551
EaeI YGGCCR 2 cut(s) 447, 595
Eam1104I CTCTTC 2 cut(s) 660, 690
EarI CTCTTC 2 cut(s) 660, 690
Eco130I CCWWGG 1 cut(s) 239
Eco31I GGTCTC 2 cut(s) 163, 612
Eco47I GGWCC 2 cut(s) 572, 746
Eco57I CTGAAG 1 cut(s) 684
EcoO109I RGGNCCY 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 239
EcoT22I ATGCAT 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 4 cut(s) 137, 486, 530, 552
FaqI GGGAC 2 cut(s) 247, 585
FatI CATG 4 cut(s) 133, 482, 526, 548
FblI GTMKAC 1 cut(s) 251
Fnu4HI GCNGC 4 cut(s) 44, 289, 633, 654
FokI GGATG 4 cut(s) 140, 318, 509, 609
Fsp4HI GCNGC 4 cut(s) 44, 289, 633, 654
FspBI CTAG 1 cut(s) 240
GlaI GCGC 1 cut(s) 432
GluI GCNGC 4 cut(s) 44, 289, 633, 654
HaeII RGCGCY 1 cut(s) 434
HaeIII GGCC 3 cut(s) 108, 449, 597
HapII CCGG 3 cut(s) 111, 302, 598
HhaI GCGC 1 cut(s) 433
Hin1II CATG 4 cut(s) 137, 486, 530, 552
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 2 cut(s) 235, 732
HpaII CCGG 3 cut(s) 111, 302, 598
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 3 cut(s) 139, 252, 749
Hpy188I TCNGA 2 cut(s) 39, 415
Hpy188III TCNNGA 1 cut(s) 335
Hpy8I GTNNAC 3 cut(s) 139, 252, 749
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 3 cut(s) 46, 506, 541
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 3 cut(s) 117, 248, 608
HpyCH4V TGCA 8 cut(s) 90, 139, 202, 270, 376, 486, 554, 606
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 536, 603
HpyF3I CTNAG 2 cut(s) 188, 711
HpySE526I ACGT 3 cut(s) 117, 248, 608
Hsp92II CATG 4 cut(s) 137, 486, 530, 552
HspAI GCGC 1 cut(s) 431
Kzo9I GATC 2 cut(s) 196, 400
LmnI GCTCC 3 cut(s) 22, 425, 650
Lsp1109I GCAGC 3 cut(s) 275, 619, 665
LweI GCATC 4 cut(s) 30, 162, 363, 500
MaeI CTAG 1 cut(s) 240
MaeII ACGT 3 cut(s) 117, 248, 608
MaeIII GTNAC 3 cut(s) 244, 680, 754
MalI GATC 2 cut(s) 198, 402
MboI GATC 2 cut(s) 196, 400
MboII GAAGA 7 cut(s) 42, 188, 199, 463, 602, 677, 707
MfeI CAATTG 1 cut(s) 437
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 2 cut(s) 141, 561
MluCI AATT 3 cut(s) 275, 409, 437
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 3 cut(s) 83, 530, 672
MnlI CCTC 8 cut(s) 6, 138, 167, 444, 490, 641, 756, 759
Mph1103I ATGCAT 1 cut(s) 378
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 627
MspA1I CMGCKG 2 cut(s) 67, 656
MspI CCGG 3 cut(s) 111, 302, 598
MspR9I CCNGG 2 cut(s) 111, 599
MunI CAATTG 1 cut(s) 437
MwoI GCNNNNNNNGC 3 cut(s) 73, 536, 603
NciI CCSGG 2 cut(s) 111, 599
NdeII GATC 2 cut(s) 196, 400
NlaIII CATG 4 cut(s) 137, 486, 530, 552
NlaIV GGNNCC 5 cut(s) 57, 109, 421, 573, 747
NmeAIII GCCGAG 1 cut(s) 475
NmuCI GTSAC 1 cut(s) 244
NsiI ATGCAT 1 cut(s) 378
PfeI GAWTC 1 cut(s) 732
PkrI GCNGC 4 cut(s) 45, 290, 634, 655
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
Ppu21I YACGTR 2 cut(s) 249, 609
Psp1406I AACGTT 1 cut(s) 117
PspN4I GGNNCC 5 cut(s) 57, 109, 421, 573, 747
PspPI GGNCC 3 cut(s) 107, 572, 746
PstI CTGCAG 1 cut(s) 204
PsuI RGATCY 1 cut(s) 196
RsaI GTAC 3 cut(s) 498, 716, 740
RsaNI GTAC 3 cut(s) 497, 715, 739
RseI CAYNNNNRTG 1 cut(s) 627
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 4 cut(s) 44, 289, 633, 654
Sau3AI GATC 2 cut(s) 196, 400
Sau96I GGNCC 3 cut(s) 107, 572, 746
ScaI AGTACT 2 cut(s) 498, 740
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 2 cut(s) 111, 599
SduI GDGCHC 2 cut(s) 141, 561
SfaNI GCATC 4 cut(s) 30, 162, 363, 500
SfcI CTRYAG 1 cut(s) 200
SinI GGWCC 2 cut(s) 572, 746
SmiMI CAYNNNNRTG 1 cut(s) 627
Sse9I AATT 3 cut(s) 275, 409, 437
SsiI CCGC 3 cut(s) 44, 65, 656
SspMI CTAG 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 109, 597
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 3 cut(s) 120, 251, 611
TaqI TCGA 4 cut(s) 50, 159, 561, 735
TasI AATT 3 cut(s) 275, 409, 437
TatI WGTACW 2 cut(s) 496, 738
TauI GCSGC 1 cut(s) 46
TfiI GAWTC 1 cut(s) 732
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 561
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 3 cut(s) 288, 632, 653
Tsp45I GTSAC 1 cut(s) 244
TspRI CASTG 1 cut(s) 561
VneI GTGCAC 1 cut(s) 137
VpaK11BI GGWCC 2 cut(s) 572, 746
XapI RAATTY 2 cut(s) 275, 409
XmaJI CCTAGG 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 251
XspI CTAG 1 cut(s) 240
ZrmI AGTACT 2 cut(s) 498, 740
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.