FvH4_6g18200

2-aminoethanethiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002785
Physical Location & Seq
Forward (+)
12011765 .. 12013089
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18200.t1

Sequence Viewer

Length: 861 bp
ATGGGAATGGAGGCTGCTGGTTCGATAGAGCAGGCTAGAGATCATTTTCATCATCATCATCATCATCATCATGTTGGTTATATGAAAAACAGGGTTATAAGCAAAAAGAAGTACTGCAGCAAGAACAGGAAGGTTAAGCATTCTTCTTCTGATTCTGCTCTGCAACGACTCTTTGTTTCGTGTAAAGACGTTTTCAAAGGCCTTGGAAATGGAACTCTTCCTTTGCCTCATCAAGTGCAGGAACTGCGCAGCGTTCTCGATAAAATTAGGCCACAAGATGTTGGGCTAAGCAACGATTTGCAGTTCTTCAAGCCTAACACTAGGGTCAAAGGGACTCCGAGGGTAACATATACAACCATATACAAGTGCAGTAACTTTTCCTTGTGCTGTTTCTTCATTCCAGCAACCGGTGTTATCCCCCTTCATAACCATCCCGGAATGACTGTCTTCAGTAAGCTTCTACTGGGCAAGATGCACATCAAATCATACGATTTGGTTGATGATCCCACCAAGAAGAATTCAGATGGTTCACAATTGAGATTGGCAAAGTTGAAAGCTGATAGTGTATTCACTGCCCCATGCAATACATCAGTACTATATCCAACAACAGGTGGCAATATCCACGCCTTCACCGCCATAACTCCTTGTGCAGTGCTTGACGTGCTCGGCCCTCCCTATTCTAAACAAGATGGTCGGGACTGCTCATATTACAGAGATCATCCCTATGCTGCTTACCCAAATGCAACAGTAACCCAAGAGGAAGGCCATTATTATGGGTGGTTGGAAGAGATTGAGGTGCCTCCTAACTCAGAAATGGATGGAATCGAGTACTTGGGTCCGCAGATTATTGACACTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

287

Amino Acids

32.06

Weight (kDa)

8.66

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 85 - 282 1.3e-73 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
Acc16I TGCGCA 1 cut(s) 248
AccB1I GGYRCC 1 cut(s) 796
AciI CCGC 2 cut(s) 633, 839
AclWI GGATC 1 cut(s) 497
AcsI RAATTY 1 cut(s) 517
AcuI CTGAAG 1 cut(s) 433
AfaI GTAC 3 cut(s) 113, 594, 830
AfiI CCNNNNNNNGG 2 cut(s) 407, 608
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 3 cut(s) 196, 310, 553
AjiI CACGTC 1 cut(s) 661
AluBI AGCT 2 cut(s) 457, 557
AluI AGCT 2 cut(s) 457, 557
Alw21I GWGCWC 1 cut(s) 666
AlwI GGATC 1 cut(s) 497
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 4 cut(s) 199, 269, 667, 763
ApeKI GCWGC 4 cut(s) 14, 117, 249, 728
ApoI RAATTY 1 cut(s) 517
AsiGI ACCGGT 1 cut(s) 407
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 2 cut(s) 668, 836
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 1 cut(s) 622
AvaII GGWCC 1 cut(s) 836
BanI GGYRCC 1 cut(s) 796
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 3 cut(s) 129, 261, 715
BccI CCATC 4 cut(s) 438, 518, 683, 812
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BcnI CCSGG 1 cut(s) 435
BfaI CTAG 3 cut(s) 36, 321, 859
BfmI CTRYAG 1 cut(s) 115
BisI GCNGC 4 cut(s) 15, 118, 250, 729
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 4 cut(s) 16, 119, 251, 730
BmcAI AGTACT 3 cut(s) 113, 594, 830
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 1 cut(s) 836
BmgBI CACGTC 1 cut(s) 661
BmgT120I GGNCC 2 cut(s) 668, 836
BmiI GGNNCC 2 cut(s) 798, 837
BmrFI CCNGG 1 cut(s) 435
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 1 cut(s) 462
BmuI ACTGGG 1 cut(s) 473
BpiI GAAGAC 1 cut(s) 439
Bpu1102I GCTNAGC 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 435
BsaBI GATNNNNATC 1 cut(s) 476
BsaJI CCNNGG 2 cut(s) 202, 338
BsaWI WCCGGW 1 cut(s) 407
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 608
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 476
BseDI CCNNGG 2 cut(s) 202, 338
BseGI GGATG 3 cut(s) 430, 718, 823
BseJI GATNNNNATC 1 cut(s) 476
BseLI CCNNNNNNNGG 2 cut(s) 407, 608
BseMII CTCAG 1 cut(s) 822
BseNI ACTGG 1 cut(s) 468
BseXI GCAGC 3 cut(s) 129, 261, 715
BsgI GTGCAG 3 cut(s) 257, 388, 669
BshFI GGCC 4 cut(s) 201, 271, 669, 765
BshNI GGYRCC 1 cut(s) 796
BshTI ACCGGT 1 cut(s) 407
BsiHKAI GWGCWC 1 cut(s) 666
BsiSI CCGG 2 cut(s) 408, 435
BslFI GGGAC 2 cut(s) 346, 710
BslI CCNNNNNNNGG 2 cut(s) 407, 608
BsmFI GGGAC 2 cut(s) 346, 710
BsmI GAATGC 1 cut(s) 139
BsnI GGCC 4 cut(s) 201, 271, 669, 765
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 3 cut(s) 40, 502, 715
Bsp1720I GCTNAGC 1 cut(s) 287
BspACI CCGC 2 cut(s) 633, 839
BspANI GGCC 4 cut(s) 201, 271, 669, 765
BspCNI CTCAG 1 cut(s) 821
BspLI GGNNCC 2 cut(s) 798, 837
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 1 cut(s) 497
BspT107I GGYRCC 1 cut(s) 796
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 1 cut(s) 468
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 2 cut(s) 202, 338
BssMI GATC 3 cut(s) 40, 502, 715
BssT1I CCWWGG 1 cut(s) 202
Bst4CI ACNGT 2 cut(s) 445, 748
Bst6I CTCTTC 2 cut(s) 222, 780
BstAPI GCANNNNNTGC 1 cut(s) 244
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 2 cut(s) 287, 808
BstF5I GGATG 3 cut(s) 430, 718, 823
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 3 cut(s) 43, 505, 718
BstMBI GATC 3 cut(s) 40, 502, 715
BstMWI GCNNNNNNNGC 3 cut(s) 244, 632, 661
BstSCI CCNGG 1 cut(s) 433
BstSFI CTRYAG 1 cut(s) 115
BstV1I GCAGC 3 cut(s) 129, 261, 715
BstV2I GAAGAC 1 cut(s) 439
BstXI CCANNNNNNTGG 1 cut(s) 773
BsuRI GGCC 4 cut(s) 201, 271, 669, 765
BtrI CACGTC 1 cut(s) 661
BtsCI GGATG 3 cut(s) 430, 718, 823
BtsI GCAGTG 2 cut(s) 570, 657
BtsIMutI CAGTG 2 cut(s) 570, 657
Cac8I GCNNGC 1 cut(s) 33
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 1 cut(s) 249
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 2 cut(s) 668, 836
Csp6I GTAC 3 cut(s) 112, 593, 829
CspAI ACCGGT 1 cut(s) 407
CviAII CATG 2 cut(s) 71, 579
CviQI GTAC 3 cut(s) 112, 593, 829
DdeI CTNAG 2 cut(s) 287, 808
DpnI GATC 3 cut(s) 42, 504, 717
DpnII GATC 3 cut(s) 40, 502, 715
Eam1104I CTCTTC 2 cut(s) 222, 780
EarI CTCTTC 2 cut(s) 222, 780
Eco130I CCWWGG 1 cut(s) 202
Eco147I AGGCCT 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 836
Eco57I CTGAAG 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 517
EcoT14I CCWWGG 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 2 cut(s) 74, 582
FaqI GGGAC 2 cut(s) 346, 710
FatI CATG 2 cut(s) 70, 578
Fnu4HI GCNGC 4 cut(s) 15, 118, 250, 729
FokI GGATG 3 cut(s) 417, 705, 830
Fsp4HI GCNGC 4 cut(s) 15, 118, 250, 729
FspBI CTAG 3 cut(s) 36, 321, 859
FspI TGCGCA 1 cut(s) 248
GlaI GCGC 1 cut(s) 248
GluI GCNGC 4 cut(s) 15, 118, 250, 729
HaeIII GGCC 4 cut(s) 201, 271, 669, 765
HapII CCGG 2 cut(s) 408, 435
HhaI GCGC 1 cut(s) 249
Hin1II CATG 2 cut(s) 74, 582
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 4 cut(s) 152, 168, 334, 822
HpaII CCGG 2 cut(s) 408, 435
HphI GGTGA 1 cut(s) 622
Hpy166II GTNNAC 1 cut(s) 530
Hpy188I TCNGA 4 cut(s) 151, 339, 523, 811
Hpy188III TCNNGA 2 cut(s) 257, 695
Hpy8I GTNNAC 1 cut(s) 530
HpyAV CCTTC 4 cut(s) 124, 431, 637, 755
HpyCH4III ACNGT 2 cut(s) 445, 748
HpyCH4IV ACGT 2 cut(s) 189, 660
HpyCH4V TGCA 9 cut(s) 117, 163, 238, 301, 369, 475, 582, 650, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 244, 632, 661
HpyF3I CTNAG 2 cut(s) 287, 808
HpySE526I ACGT 2 cut(s) 189, 660
Hsp92II CATG 2 cut(s) 74, 582
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 3 cut(s) 40, 502, 715
Lsp1109I GCAGC 3 cut(s) 129, 261, 715
LweI GCATC 1 cut(s) 462
MaeI CTAG 3 cut(s) 36, 321, 859
MaeII ACGT 2 cut(s) 189, 660
MaeIII GTNAC 3 cut(s) 343, 371, 748
MalI GATC 3 cut(s) 42, 504, 717
MboI GATC 3 cut(s) 40, 502, 715
MboII GAAGA 8 cut(s) 135, 138, 209, 298, 385, 439, 526, 797
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 666
MluCI AATT 3 cut(s) 264, 517, 533
MlyI GAGTC 2 cut(s) 162, 328
MmeI TCCRAC 2 cut(s) 626, 762
MnlI CCTC 7 cut(s) 4, 237, 333, 681, 751, 787, 810
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 3 cut(s) 69, 723, 771
MspI CCGG 2 cut(s) 408, 435
MspR9I CCNGG 1 cut(s) 435
MunI CAATTG 1 cut(s) 533
Mva1269I GAATGC 1 cut(s) 139
MwoI GCNNNNNNNGC 3 cut(s) 244, 632, 661
NciI CCSGG 1 cut(s) 435
NdeII GATC 3 cut(s) 40, 502, 715
NlaIII CATG 2 cut(s) 74, 582
NlaIV GGNNCC 2 cut(s) 798, 837
NmeAIII GCCGAG 1 cut(s) 645
NsbI TGCGCA 1 cut(s) 248
PceI AGGCCT 1 cut(s) 201
PctI GAATGC 1 cut(s) 139
PfeI GAWTC 2 cut(s) 152, 822
PfoI TCCNGGA 1 cut(s) 433
PinAI ACCGGT 1 cut(s) 407
PkrI GCNGC 4 cut(s) 16, 119, 251, 730
PleI GAGTC 2 cut(s) 162, 328
PpsI GAGTC 2 cut(s) 162, 328
PsiI TTATAA 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 798, 837
PspPI GGNCC 2 cut(s) 668, 836
PstI CTGCAG 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 244
RsaI GTAC 3 cut(s) 113, 594, 830
RsaNI GTAC 3 cut(s) 112, 593, 829
RseI CAYNNNNRTG 3 cut(s) 69, 723, 771
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 4 cut(s) 15, 118, 250, 729
Sau3AI GATC 3 cut(s) 40, 502, 715
Sau96I GGNCC 2 cut(s) 668, 836
ScaI AGTACT 3 cut(s) 113, 594, 830
SchI GAGTC 2 cut(s) 162, 328
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 666
SetI ASST 7 cut(s) 135, 192, 459, 559, 613, 663, 798
SfaNI GCATC 1 cut(s) 462
SfcI CTRYAG 1 cut(s) 115
SinI GGWCC 1 cut(s) 836
SmiMI CAYNNNNRTG 3 cut(s) 69, 723, 771
Sse9I AATT 3 cut(s) 264, 517, 533
SseBI AGGCCT 1 cut(s) 201
SsiI CCGC 2 cut(s) 633, 839
SspMI CTAG 3 cut(s) 36, 321, 859
StuI AGGCCT 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 2 cut(s) 445, 748
TaiI ACGT 2 cut(s) 192, 663
TaqI TCGA 3 cut(s) 23, 258, 825
TasI AATT 3 cut(s) 264, 517, 533
TatI WGTACW 3 cut(s) 111, 592, 828
TfiI GAWTC 2 cut(s) 152, 822
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 2 cut(s) 577, 657
TseI GCWGC 4 cut(s) 14, 117, 249, 728
TspDTI ATGAA 4 cut(s) 38, 98, 385, 413
TspRI CASTG 2 cut(s) 577, 657
VpaK11BI GGWCC 1 cut(s) 836
XapI RAATTY 1 cut(s) 517
XspI CTAG 3 cut(s) 36, 321, 859
ZrmI AGTACT 3 cut(s) 113, 594, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.