MD12G1009200.v1.1

2-aminoethanethiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002785
Physical Location & Seq
Forward (+)
966983 .. 971554
4572 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1009200.v1.1.491

Sequence Viewer

Length: 732 bp
ATGAGACCAGAAGACATTGGGCTGAGTAGGAATCTGCAGTTCTTCAAGCCTAAAACTGTTGTGAAGGGGACTCCTAGGGTCACGTATACAACCATATATGAGTGCAATAAATTTTCGTTGTGCTGCTTTTTTATTCCGGCAAATGGCGTTATCCCACTCCATAACCATCCATTTATGACTGTTTTCAGCAAGCTTCTACTAGGCAAGATGCATATTAAATCATACGATTGGGTTGATCCCGTTAATTCAGATGGCTCCACGCCAGCCCCACAATTGAGACTGGCCGAGTTGAAAGCTGATAGTGTCTTCACAGCCCCTTGCAATGCATCAGTACTATATCCAACAAAAGGTGGCAACATCCATGCATTCACGGCCATAACACCATGTGCAGTGCTCGATGTGATTGGACCTCCGTATTCTAAAGAAGATGGCCGGGATTGCTCATATGACAAAGATCATCCCTATGCTGCATCTTCAAATGGAGGAGCAGCAGTAACTGAAGAGAAACACGATTGTTATGGATGGTTGGAAGAGATTAAAATGCCTGAGAACGCAGAAATGGATATAATTGAGTACTTGGGTCCACAAGTTAGTGAGACGAATGGAGGAGCAGCAGTAACTGAAGAGAAACGCGATTGTTATGGATGGTTGGAAGAGATTGAAATGCCTGAGAACGCAGAAATGGATATGATTGAGTACTTGGGTCCACAAGTTAGTGAGACGAGTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

244

Amino Acids

26.92

Weight (kDa)

4.75

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 1 - 197 3e-67 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 86
AccII CGCG 1 cut(s) 633
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 3 cut(s) 282, 372, 430
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 2 cut(s) 519, 642
AdeI CACNNNGTG 1 cut(s) 386
AfaI GTAC 3 cut(s) 333, 575, 698
AfiI CCNNNNNNNGG 2 cut(s) 143, 347
AgsI TTSAA 4 cut(s) 46, 292, 477, 662
AluBI AGCT 2 cut(s) 193, 296
AluI AGCT 2 cut(s) 193, 296
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 3 cut(s) 271, 590, 713
AlwI GGATC 1 cut(s) 230
AlwNI CAGNNNCTG 2 cut(s) 497, 620
AoxI GGCC 3 cut(s) 282, 372, 430
ApeKI GCWGC 4 cut(s) 123, 467, 488, 611
ApoI RAATTY 1 cut(s) 110
AspA2I CCTAGG 1 cut(s) 74
AspS9I GGNCC 3 cut(s) 407, 581, 704
AsuC2I CCSGG 1 cut(s) 434
AvaII GGWCC 3 cut(s) 407, 581, 704
AvrII CCTAGG 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 18, 298
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 4 cut(s) 110, 454, 500, 623
BccI CCATC 5 cut(s) 174, 245, 422, 516, 639
BceAI ACGGC 1 cut(s) 387
BcnI CCSGG 1 cut(s) 434
BcoDI GTCTC 3 cut(s) 271, 590, 713
BfaI CTAG 2 cut(s) 75, 200
BfmI CTRYAG 1 cut(s) 35
BisI GCNGC 4 cut(s) 124, 468, 489, 612
BlnI CCTAGG 1 cut(s) 74
BlsI GCNGC 4 cut(s) 125, 469, 490, 613
BmcAI AGTACT 3 cut(s) 333, 575, 698
Bme1390I CCNGG 1 cut(s) 434
Bme18I GGWCC 3 cut(s) 407, 581, 704
BmgT120I GGNCC 3 cut(s) 407, 581, 704
BmiI GGNNCC 3 cut(s) 256, 582, 705
BmrFI CCNGG 1 cut(s) 434
BmsI GCATC 3 cut(s) 198, 335, 479
BpiI GAAGAC 2 cut(s) 18, 298
BpuMI CCSGG 1 cut(s) 434
BsaAI YACGTR 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 74
BsaXI ACNNNNNCTCC 4 cut(s) 477, 507, 600, 630
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 347
Bse1I ACTGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 328
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 5 cut(s) 166, 357, 457, 527, 650
BseLI CCNNNNNNNGG 2 cut(s) 143, 347
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 3 cut(s) 14, 537, 660
BseNI ACTGG 1 cut(s) 285
BseRI GAGGAG 2 cut(s) 498, 621
BseXI GCAGC 4 cut(s) 110, 454, 500, 623
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 1 cut(s) 633
BshFI GGCC 3 cut(s) 284, 374, 432
BsiHKAI GWGCWC 1 cut(s) 396
BsiSI CCGG 2 cut(s) 137, 433
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 2 cut(s) 143, 347
BsmAI GTCTC 3 cut(s) 271, 590, 713
BsmBI CGTCTC 2 cut(s) 590, 713
BsmFI GGGAC 1 cut(s) 82
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 3 cut(s) 284, 374, 432
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 2 cut(s) 235, 454
BspANI GGCC 3 cut(s) 284, 374, 432
BspCNI CTCAG 3 cut(s) 15, 538, 661
BspFNI CGCG 1 cut(s) 633
BspLI GGNNCC 3 cut(s) 256, 582, 705
BspMAI CTGCAG 1 cut(s) 39
BspPI GGATC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 328
BsrI ACTGG 1 cut(s) 285
BssECI CCNNGG 1 cut(s) 74
BssMI GATC 2 cut(s) 235, 454
BssNAI GTATAC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 74
Bst1107I GTATAC 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 58, 181
Bst6I CTCTTC 4 cut(s) 495, 525, 618, 648
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 191, 264
BstDEI CTNAG 3 cut(s) 23, 546, 669
BstF5I GGATG 5 cut(s) 166, 357, 457, 527, 650
BstFNI CGCG 1 cut(s) 633
BstKTI GATC 2 cut(s) 238, 457
BstMAI GTCTC 3 cut(s) 271, 590, 713
BstMBI GATC 2 cut(s) 235, 454
BstMWI GCNNNNNNNGC 2 cut(s) 371, 438
BstSCI CCNGG 1 cut(s) 432
BstSFI CTRYAG 1 cut(s) 35
BstUI CGCG 1 cut(s) 633
BstV1I GCAGC 4 cut(s) 110, 454, 500, 623
BstV2I GAAGAC 2 cut(s) 18, 298
BstZ17I GTATAC 1 cut(s) 87
BsuRI GGCC 3 cut(s) 284, 374, 432
BtsCI GGATG 5 cut(s) 166, 357, 457, 527, 650
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 191, 264
CaiI CAGNNNCTG 2 cut(s) 497, 620
Cfr13I GGNCC 3 cut(s) 407, 581, 704
Csp6I GTAC 3 cut(s) 332, 574, 697
CviAII CATG 2 cut(s) 362, 384
CviQI GTAC 3 cut(s) 332, 574, 697
DdeI CTNAG 3 cut(s) 23, 546, 669
DpnI GATC 2 cut(s) 237, 456
DpnII GATC 2 cut(s) 235, 454
DraIII CACNNNGTG 1 cut(s) 386
EaeI YGGCCR 3 cut(s) 282, 372, 430
Eam1104I CTCTTC 4 cut(s) 495, 525, 618, 648
EarI CTCTTC 4 cut(s) 495, 525, 618, 648
Eco130I CCWWGG 1 cut(s) 74
Eco47I GGWCC 3 cut(s) 407, 581, 704
Eco57I CTGAAG 2 cut(s) 519, 642
EcoT14I CCWWGG 1 cut(s) 74
EcoT22I ATGCAT 3 cut(s) 213, 328, 367
ErhI CCWWGG 1 cut(s) 74
Esp3I CGTCTC 2 cut(s) 590, 713
FaeI CATG 2 cut(s) 365, 387
FaqI GGGAC 1 cut(s) 82
FatI CATG 2 cut(s) 361, 383
FauNDI CATATG 1 cut(s) 445
FblI GTMKAC 1 cut(s) 86
Fnu4HI GCNGC 4 cut(s) 124, 468, 489, 612
FokI GGATG 5 cut(s) 153, 344, 444, 534, 657
Fsp4HI GCNGC 4 cut(s) 124, 468, 489, 612
FspBI CTAG 2 cut(s) 75, 200
GluI GCNGC 4 cut(s) 124, 468, 489, 612
HaeIII GGCC 3 cut(s) 284, 374, 432
HapII CCGG 2 cut(s) 137, 433
Hin1II CATG 2 cut(s) 365, 387
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 2 cut(s) 31, 70
HpaII CCGG 2 cut(s) 137, 433
Hpy166II GTNNAC 3 cut(s) 87, 584, 707
Hpy188I TCNGA 1 cut(s) 250
Hpy8I GTNNAC 3 cut(s) 87, 584, 707
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 2 cut(s) 58, 181
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 8 cut(s) 37, 105, 211, 321, 326, 365, 389, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 438
HpyF3I CTNAG 3 cut(s) 23, 546, 669
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 2 cut(s) 365, 387
Kzo9I GATC 2 cut(s) 235, 454
LmnI GCTCC 3 cut(s) 260, 485, 608
LpnPI CCDG 7 cut(s) 21, 150, 266, 276, 446, 558, 681
Lsp1109I GCAGC 4 cut(s) 110, 454, 500, 623
LweI GCATC 3 cut(s) 198, 335, 479
MaeI CTAG 2 cut(s) 75, 200
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 3 cut(s) 79, 493, 616
MalI GATC 2 cut(s) 237, 456
MboI GATC 2 cut(s) 235, 454
MboII GAAGA 9 cut(s) 23, 34, 298, 437, 465, 512, 542, 635, 665
MfeI CAATTG 1 cut(s) 272
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 4 cut(s) 110, 244, 272, 567
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 3 cut(s) 365, 507, 630
MnlI CCTC 3 cut(s) 420, 476, 599
Mph1103I ATGCAT 3 cut(s) 213, 328, 367
MseI TTAA 3 cut(s) 216, 243, 537
MslI CAYNNNNRTG 1 cut(s) 462
MspI CCGG 2 cut(s) 137, 433
MspR9I CCNGG 1 cut(s) 434
MunI CAATTG 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 365
MvnI CGCG 1 cut(s) 633
MwoI GCNNNNNNNGC 2 cut(s) 371, 438
NciI CCSGG 1 cut(s) 434
NdeI CATATG 1 cut(s) 445
NdeII GATC 2 cut(s) 235, 454
NlaIII CATG 2 cut(s) 365, 387
NlaIV GGNNCC 3 cut(s) 256, 582, 705
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 79
NsiI ATGCAT 3 cut(s) 213, 328, 367
PctI GAATGC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 4 cut(s) 125, 469, 490, 613
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
Ppu21I YACGTR 1 cut(s) 84
PspN4I GGNNCC 3 cut(s) 256, 582, 705
PspPI GGNCC 3 cut(s) 407, 581, 704
PstI CTGCAG 1 cut(s) 39
PstNI CAGNNNCTG 2 cut(s) 497, 620
RsaI GTAC 3 cut(s) 333, 575, 698
RsaNI GTAC 3 cut(s) 332, 574, 697
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 3 cut(s) 216, 243, 537
SatI GCNGC 4 cut(s) 124, 468, 489, 612
Sau3AI GATC 2 cut(s) 235, 454
Sau96I GGNCC 3 cut(s) 407, 581, 704
ScaI AGTACT 3 cut(s) 333, 575, 698
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 434
SduI GDGCHC 1 cut(s) 396
SetI ASST 5 cut(s) 86, 195, 298, 352, 412
SfaNI GCATC 3 cut(s) 198, 335, 479
SfcI CTRYAG 1 cut(s) 35
SinI GGWCC 3 cut(s) 407, 581, 704
SmiMI CAYNNNNRTG 1 cut(s) 462
Sse9I AATT 4 cut(s) 110, 244, 272, 567
SspMI CTAG 2 cut(s) 75, 200
StyD4I CCNGG 1 cut(s) 432
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 2 cut(s) 58, 181
TaiI ACGT 1 cut(s) 86
TaqI TCGA 1 cut(s) 396
TasI AATT 4 cut(s) 110, 244, 272, 567
TatI WGTACW 3 cut(s) 331, 573, 696
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 3 cut(s) 216, 243, 537
Tru9I TTAA 3 cut(s) 216, 243, 537
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 4 cut(s) 123, 467, 488, 611
Tsp45I GTSAC 1 cut(s) 79
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 1 cut(s) 396
VpaK11BI GGWCC 3 cut(s) 407, 581, 704
XapI RAATTY 1 cut(s) 110
XmaJI CCTAGG 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 86
XspI CTAG 2 cut(s) 75, 200
ZrmI AGTACT 3 cut(s) 333, 575, 698
Zsp2I ATGCAT 3 cut(s) 213, 328, 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.