MD12G1009100.v1.1

2-aminoethanethiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002785
Physical Location & Seq
Forward (+)
960422 .. 963044
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1009100.v1.1.491

Sequence Viewer

Length: 894 bp
ATGACTATGGAGACTGCGAGAGTGGTGGAGCAGGGAAGACATCAGAGGGTTGTTTCGCGTGTTGGGCACGCTAGCAGCAGCAGCAAGGTTAGGTATCAAGTGAAACAGGGTATAAGGAAGAGAAAATGGAGCCGGAAGATTCAGCATCCTCTTTCGTTGGCGCCGCCGCCGACTGTTCCAACCCCGCTGCAACAGCTGTTTGATGCGTGCAGACATGTTTTCAAAGGCCATGGAACCGTTCCTTTGCCTCATCATGTGCACAAGCTGTGTCGCATTCTCGATGATATGACACCAGAAGACATTGGGCTGAGTAGGAATCTGCAGTTCTTCAAGCCTAAGGCTGTTGTGAAAGGGACTCCTAGGGTCACGTATACGACCATATACGAGTGCAATAAATTTTCGTTGTGCTGCTTTTTTATTCCGGCAAATGGCGTTATCCCACTCCATAACCATCCAGATATGACTGTTTTCAGCAAGCTTCTACTAGGCAAGATGCATATTAAATCATACGATTGGGTTGATCCCGTTAATTCAGATGGCTCCACGCCAGCCTCTCAATTGAGACTGGCCGAGTTGAAAGCTGATAGCGTCTTCACAGCCCCTTGCAATGCATCGGTACTCTATCCAACAAAAGGTGGCAACATCCATGCCTTCACAGCCATAACACCATGTGCAGTGCTCGATGTGATTGGGCCTCCGTATTCTAAAGAAGATGGCCGGGATTGCTCCTATTACAAAGATCATCCCTATGCTGCATATTCAAATGGAGGAGCAGCAGTAACTGAAGAGAAACGCGATTGTTATGGATGGTTGGAAGAGATTGAAATGCCTGAGAACGCAGAAATGGATATAATTGAGTACTTGGGTCCACAAGTTAGTGAGACGAGTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

298

Amino Acids

33.17

Weight (kDa)

8.49

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 92 - 292 9.8e-72 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 160
AccI GTMKAC 1 cut(s) 371
AccII CGCG 2 cut(s) 58, 795
AciI CCGC 3 cut(s) 164, 167, 185
AclWI GGATC 1 cut(s) 515
AcoI YGGCCR 2 cut(s) 567, 715
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 804
AcyI GRCGYC 1 cut(s) 161
AdeI CACNNNGTG 1 cut(s) 671
AfaI GTAC 2 cut(s) 618, 860
AfiI CCNNNNNNNGG 2 cut(s) 428, 632
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 5 cut(s) 223, 331, 577, 762, 824
AluBI AGCT 4 cut(s) 196, 265, 478, 581
AluI AGCT 4 cut(s) 196, 265, 478, 581
Alw21I GWGCWC 2 cut(s) 261, 681
Alw26I GTCTC 3 cut(s) 5, 556, 875
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 1 cut(s) 515
AlwNI CAGNNNCTG 1 cut(s) 782
AoxI GGCC 4 cut(s) 226, 567, 692, 715
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 7 cut(s) 75, 78, 81, 187, 408, 752, 773
ApoI RAATTY 1 cut(s) 395
AspA2I CCTAGG 1 cut(s) 359
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 2 cut(s) 692, 866
AsuC2I CCSGG 1 cut(s) 719
AsuNHI GCTAGC 1 cut(s) 71
AvaII GGWCC 1 cut(s) 866
AvrII CCTAGG 1 cut(s) 359
AxyI CCTNAGG 1 cut(s) 336
BaeGI GKGCMC 2 cut(s) 69, 261
BanI GGYRCC 1 cut(s) 160
BbsI GAAGAC 3 cut(s) 43, 303, 583
Bbv12I GWGCWC 2 cut(s) 261, 681
BbvI GCAGC 7 cut(s) 87, 90, 93, 174, 395, 739, 785
BccI CCATC 4 cut(s) 459, 530, 707, 801
BcnI CCSGG 1 cut(s) 719
BcoDI GTCTC 3 cut(s) 5, 556, 875
BfaI CTAG 3 cut(s) 72, 360, 485
BfmI CTRYAG 1 cut(s) 320
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 9 cut(s) 76, 79, 82, 164, 167, 188, 409, 753, 774
BlnI CCTAGG 1 cut(s) 359
BlsI GCNGC 9 cut(s) 77, 80, 83, 165, 168, 189, 410, 754, 775
BmcAI AGTACT 1 cut(s) 860
Bme1390I CCNGG 1 cut(s) 719
Bme18I GGWCC 1 cut(s) 866
BmgT120I GGNCC 2 cut(s) 692, 866
BmiI GGNNCC 5 cut(s) 131, 162, 235, 541, 867
BmrFI CCNGG 1 cut(s) 719
BmsI GCATC 4 cut(s) 154, 193, 483, 620
BmtI GCTAGC 1 cut(s) 75
BpiI GAAGAC 3 cut(s) 43, 303, 583
BpuMI CCSGG 1 cut(s) 719
BsaAI YACGTR 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 161
BsaJI CCNNGG 2 cut(s) 229, 359
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 632
Bse1I ACTGG 1 cut(s) 570
Bse21I CCTNAGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 613
BseDI CCNNGG 2 cut(s) 229, 359
BseGI GGATG 5 cut(s) 145, 451, 642, 742, 812
BseLI CCNNNNNNNGG 2 cut(s) 428, 632
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 2 cut(s) 299, 822
BseNI ACTGG 1 cut(s) 570
BseRI GAGGAG 1 cut(s) 783
BseSI GKGCMC 2 cut(s) 69, 261
BseXI GCAGC 7 cut(s) 87, 90, 93, 174, 395, 739, 785
BsgI GTGCAG 2 cut(s) 229, 693
Bsh1236I CGCG 2 cut(s) 58, 795
BshFI GGCC 4 cut(s) 228, 569, 694, 717
BshNI GGYRCC 1 cut(s) 160
BsiHKAI GWGCWC 2 cut(s) 261, 681
BsiSI CCGG 3 cut(s) 133, 422, 718
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 2 cut(s) 428, 632
BsmAI GTCTC 3 cut(s) 5, 556, 875
BsmBI CGTCTC 1 cut(s) 875
BsmFI GGGAC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 4 cut(s) 228, 569, 694, 717
Bsp1286I GDGCHC 3 cut(s) 69, 261, 681
Bsp143I GATC 2 cut(s) 520, 739
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 3 cut(s) 164, 167, 185
BspANI GGCC 4 cut(s) 228, 569, 694, 717
BspCNI CTCAG 2 cut(s) 300, 823
BspFNI CGCG 2 cut(s) 58, 795
BspLI GGNNCC 5 cut(s) 131, 162, 235, 541, 867
BspMAI CTGCAG 1 cut(s) 324
BspOI GCTAGC 1 cut(s) 75
BspPI GGATC 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 613
BsrI ACTGG 1 cut(s) 570
BssECI CCNNGG 2 cut(s) 229, 359
BssMI GATC 2 cut(s) 520, 739
BssNAI GTATAC 1 cut(s) 372
BssNI GRCGYC 1 cut(s) 161
BssT1I CCWWGG 2 cut(s) 229, 359
Bst1107I GTATAC 1 cut(s) 372
Bst4CI ACNGT 3 cut(s) 175, 238, 466
Bst6I CTCTTC 3 cut(s) 113, 780, 810
BstACI GRCGYC 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 369
BstC8I GCNNGC 5 cut(s) 69, 73, 208, 476, 549
BstDEI CTNAG 3 cut(s) 308, 336, 831
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 5 cut(s) 145, 451, 642, 742, 812
BstFNI CGCG 2 cut(s) 58, 795
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 2 cut(s) 523, 742
BstMAI GTCTC 3 cut(s) 5, 556, 875
BstMBI GATC 2 cut(s) 520, 739
BstMWI GCNNNNNNNGC 5 cut(s) 64, 81, 193, 656, 723
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 717
BstSFI CTRYAG 1 cut(s) 320
BstSLI GKGCMC 2 cut(s) 69, 261
BstUI CGCG 2 cut(s) 58, 795
BstV1I GCAGC 7 cut(s) 87, 90, 93, 174, 395, 739, 785
BstV2I GAAGAC 3 cut(s) 43, 303, 583
BstZ17I GTATAC 1 cut(s) 372
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 4 cut(s) 228, 569, 694, 717
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 5 cut(s) 145, 451, 642, 742, 812
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 5 cut(s) 69, 73, 208, 476, 549
CaiI CAGNNNCTG 1 cut(s) 782
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 2 cut(s) 692, 866
CseI GACGC 1 cut(s) 577
Csp6I GTAC 2 cut(s) 617, 859
CviAII CATG 5 cut(s) 215, 230, 254, 647, 669
CviQI GTAC 2 cut(s) 617, 859
DdeI CTNAG 3 cut(s) 308, 336, 831
DinI GGCGCC 1 cut(s) 162
DpnI GATC 2 cut(s) 522, 741
DpnII GATC 2 cut(s) 520, 739
DraIII CACNNNGTG 1 cut(s) 671
EaeI YGGCCR 2 cut(s) 567, 715
Eam1104I CTCTTC 3 cut(s) 113, 780, 810
EarI CTCTTC 3 cut(s) 113, 780, 810
Eco130I CCWWGG 2 cut(s) 229, 359
Eco47I GGWCC 1 cut(s) 866
Eco57I CTGAAG 1 cut(s) 804
Eco81I CCTNAGG 1 cut(s) 336
EcoT14I CCWWGG 2 cut(s) 229, 359
EcoT22I ATGCAT 2 cut(s) 498, 613
EgeI GGCGCC 1 cut(s) 162
EheI GGCGCC 1 cut(s) 162
ErhI CCWWGG 2 cut(s) 229, 359
Esp3I CGTCTC 1 cut(s) 875
FaeI CATG 5 cut(s) 218, 233, 257, 650, 672
FaqI GGGAC 1 cut(s) 367
FatI CATG 5 cut(s) 214, 229, 253, 646, 668
FauI CCCGC 1 cut(s) 192
FblI GTMKAC 1 cut(s) 371
Fnu4HI GCNGC 9 cut(s) 76, 79, 82, 164, 167, 188, 409, 753, 774
FokI GGATG 5 cut(s) 132, 438, 629, 729, 819
Fsp4HI GCNGC 9 cut(s) 76, 79, 82, 164, 167, 188, 409, 753, 774
FspBI CTAG 3 cut(s) 72, 360, 485
GlaI GCGC 1 cut(s) 162
GluI GCNGC 9 cut(s) 76, 79, 82, 164, 167, 188, 409, 753, 774
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 4 cut(s) 228, 569, 694, 717
HapII CCGG 3 cut(s) 133, 422, 718
HgaI GACGC 1 cut(s) 577
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 161
Hin1II CATG 5 cut(s) 218, 233, 257, 650, 672
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 3 cut(s) 139, 316, 355
HpaII CCGG 3 cut(s) 133, 422, 718
Hpy166II GTNNAC 3 cut(s) 259, 372, 869
Hpy188I TCNGA 2 cut(s) 45, 535
Hpy188III TCNNGA 2 cut(s) 278, 455
Hpy8I GTNNAC 3 cut(s) 259, 372, 869
HpyAV CCTTC 1 cut(s) 661
HpyCH4III ACNGT 3 cut(s) 175, 238, 466
HpyCH4IV ACGT 1 cut(s) 368
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 81, 193, 656, 723
HpyF3I CTNAG 3 cut(s) 308, 336, 831
HpySE526I ACGT 1 cut(s) 368
Hsp92I GRCGYC 1 cut(s) 161
Hsp92II CATG 5 cut(s) 218, 233, 257, 650, 672
HspAI GCGC 1 cut(s) 161
KasI GGCGCC 1 cut(s) 160
Kzo9I GATC 2 cut(s) 520, 739
LmnI GCTCC 5 cut(s) 28, 129, 545, 731, 770
Lsp1109I GCAGC 7 cut(s) 87, 90, 93, 174, 395, 739, 785
LweI GCATC 4 cut(s) 154, 193, 483, 620
MaeI CTAG 3 cut(s) 72, 360, 485
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 2 cut(s) 364, 778
MalI GATC 2 cut(s) 522, 741
MboI GATC 2 cut(s) 520, 739
MboII GAAGA 9 cut(s) 48, 130, 148, 308, 319, 583, 722, 797, 827
MfeI CAATTG 1 cut(s) 557
MhlI GDGCHC 3 cut(s) 69, 261, 681
MluCI AATT 4 cut(s) 395, 529, 557, 852
Mly113I GGCGCC 1 cut(s) 161
MlyI GAGTC 1 cut(s) 349
MmeI TCCRAC 3 cut(s) 203, 650, 792
MnlI CCTC 6 cut(s) 39, 159, 258, 562, 705, 761
Mph1103I ATGCAT 2 cut(s) 498, 613
MseI TTAA 2 cut(s) 501, 528
MslI CAYNNNNRTG 1 cut(s) 747
MspA1I CMGCKG 2 cut(s) 187, 196
MspI CCGG 3 cut(s) 133, 422, 718
MspR9I CCNGG 1 cut(s) 719
MunI CAATTG 1 cut(s) 557
Mva1269I GAATGC 1 cut(s) 273
MvnI CGCG 2 cut(s) 58, 795
MwoI GCNNNNNNNGC 5 cut(s) 64, 81, 193, 656, 723
NarI GGCGCC 1 cut(s) 161
NciI CCSGG 1 cut(s) 719
NcoI CCATGG 1 cut(s) 229
NdeII GATC 2 cut(s) 520, 739
NheI GCTAGC 1 cut(s) 71
NlaIII CATG 5 cut(s) 218, 233, 257, 650, 672
NlaIV GGNNCC 5 cut(s) 131, 162, 235, 541, 867
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 1 cut(s) 364
NsiI ATGCAT 2 cut(s) 498, 613
NspI RCATGY 1 cut(s) 218
PciI ACATGT 1 cut(s) 214
PctI GAATGC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 139, 316
PkrI GCNGC 9 cut(s) 77, 80, 83, 165, 168, 189, 410, 754, 775
PleI GAGTC 1 cut(s) 349
PluTI GGCGCC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 349
Ppu21I YACGTR 1 cut(s) 369
PscI ACATGT 1 cut(s) 214
PspN4I GGNNCC 5 cut(s) 131, 162, 235, 541, 867
PspPI GGNCC 2 cut(s) 692, 866
PstI CTGCAG 1 cut(s) 324
PstNI CAGNNNCTG 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 2 cut(s) 618, 860
RsaNI GTAC 2 cut(s) 617, 859
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 2 cut(s) 501, 528
SatI GCNGC 9 cut(s) 76, 79, 82, 164, 167, 188, 409, 753, 774
Sau3AI GATC 2 cut(s) 520, 739
Sau96I GGNCC 2 cut(s) 692, 866
ScaI AGTACT 1 cut(s) 860
SchI GAGTC 1 cut(s) 349
ScrFI CCNGG 1 cut(s) 719
SduI GDGCHC 3 cut(s) 69, 261, 681
SetI ASST 8 cut(s) 90, 95, 198, 267, 371, 480, 583, 637
SfaNI GCATC 4 cut(s) 154, 193, 483, 620
SfcI CTRYAG 1 cut(s) 320
SfoI GGCGCC 1 cut(s) 162
SinI GGWCC 1 cut(s) 866
SmiMI CAYNNNNRTG 1 cut(s) 747
Sse9I AATT 4 cut(s) 395, 529, 557, 852
SsiI CCGC 3 cut(s) 164, 167, 185
SspDI GGCGCC 1 cut(s) 160
SspMI CTAG 3 cut(s) 72, 360, 485
StyD4I CCNGG 1 cut(s) 717
StyI CCWWGG 2 cut(s) 229, 359
TaaI ACNGT 3 cut(s) 175, 238, 466
TaiI ACGT 1 cut(s) 371
TaqI TCGA 2 cut(s) 279, 681
TasI AATT 4 cut(s) 395, 529, 557, 852
TatI WGTACW 1 cut(s) 858
TauI GCSGC 2 cut(s) 166, 169
TfiI GAWTC 2 cut(s) 139, 316
Tru1I TTAA 2 cut(s) 501, 528
Tru9I TTAA 2 cut(s) 501, 528
TscAI CASTG 1 cut(s) 681
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 7 cut(s) 75, 78, 81, 187, 408, 752, 773
Tsp45I GTSAC 1 cut(s) 364
TspGWI ACGGA 1 cut(s) 687
TspRI CASTG 1 cut(s) 681
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 866
XapI RAATTY 1 cut(s) 395
XceI RCATGY 1 cut(s) 218
XmaJI CCTAGG 1 cut(s) 359
XmiI GTMKAC 1 cut(s) 371
XspI CTAG 3 cut(s) 72, 360, 485
ZrmI AGTACT 1 cut(s) 860
Zsp2I ATGCAT 2 cut(s) 498, 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.