AT5G61250

Heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004486
Physical Location & Seq
Reverse (-)
24632154 .. 24635312
3159 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61250.3

Sequence Viewer

Length: 1620 bp
ATGGGCTTCAACGTTGTTGTGTTCTTGAGTTGTCTGCTTCTACTTCCTCCTGTTACTTTTGGTAGCAACATGGAACGAACCACCTTGGTTATAGACGGCTCACGTCGAATCGCTGAGACCGATGAGAACTTTATCTGCGCAACGCTTGATTGGTGGCCACCTGAAAAATGCAACTATGATCAATGTCCTTGGGGTTACGCATCTCTCATCAATTTGAACTTAGCTTCTCCTCTTCTTGCTAAAGCTATTCAAGCTTTTAGGACGTTGAGGATAAGAATAGGTGGTTCCTTGCAAGATCAAGTGATCTACGACGTTGGAGACTTGAAGACTCCTTGCACTCAGTTCAAGAAAACTGACGACGGATTGTTCGGATTCTCTGAAGGATGTTTGTATATGAAACGTTGGGACGAAGTTAACCATTTCTTCAACGCAACGGGAGCTATTGTGACTTTCGGTTTGAACGCGCTGCACGGGAGAAATAAACTCAACGGAACTGCGTGGGGAGGTGATTGGGATCATACCAACACTCAGGATTTCATGAACTACACAGTCTCAAAGGGTTATGCTATAGACTCGTGGGAGTTTGGTAATGAGCTTAGTGGAAGCGGGATTTGGGCAAGCGTGAGTGTAGAGCTTTATGGGAAAGACTTGATTGTACTGAAAAATGTAATCAAGAACGTTTACAAGAATTCTAGAACCAAGCCTTTGGTTGTAGCTCCAGGTGGATTCTTTGAAGAACAATGGTACTCGGAGCTTCTTCGGCTCTCTGGGCCTGGTGTTCTTGATGTCCTGACTCATCATATATACAATCTTGGTCCAGGGAATGATCCTAAGCTAGTGAATAAGATACTAGATCCAAATTACTTGAGCGGAATTTCGGAATTATTCGCGAATGTGAACCAAACAATTCAAGAACATGGACCTTGGGCTGCTGCTTGGGTTGGAGAAGCTGGTGGAGCCTTTAACAGTGGTGGCCGTCAGGTTTCCGAGACATTCATTAACAGTTTCTGGTACTTAGATCAGCTCGGTATTTCGTCGAAGCATAACACCAAAGTATACTGCAGACAAGCTTTGGTCGGAGGTTTCTACGGTCTGCTTGAAAAGGAAACGTTTGTTCCAAATCCAGATTACTACAGCGCGCTTCTTTGGCATCGTTTGATGGGTAAAGGCATTCTCGGCGTTCAGACGACTGCCTCGGAGTATCTACGAGCTTACGTTCATTGCTCCAAAAGAAGAGCGGGTATAACGATTCTTCTGATCAATCTGAGTAAGCACACGACGTTTACAGTCGCAGTCAGCAATGGCGTGAAGGTGGTTTTGCAAGCAGAATCGATGAAGAGGAAATCGTTCTTGGAAACAATCAAGAGCAAGGTTTCTTGGGTTGGAAACAAAGCTTCGGACGGATATTTGAACAGAGAAGAGTATCACTTGAGTCCCAAAGACGGTGACTTGCGTAGCAAGATAATGCTCTTGAATGGTAAACCTTTGGTACCGACAGCCACCGGAGATATCCCAAAGCTTGAGCCGGTCCGTCATGGTGTGAAATCTCCGGTTTATATCAATCCGCTGTCGATCTCTTTCATTGTGTTGCCTACCTTTGATGCTCCTGCTTGTTCTTGA

Protein Analysis

539

Amino Acids

59.86

Weight (kDa)

8.22

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 27 - 343 8.7e-162 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 139
Acc65I GGTACC 1 cut(s) 1489
AccB1I GGYRCC 1 cut(s) 1489
AccBSI CCGCTC 2 cut(s) 870, 1238
AccI GTMKAC 1 cut(s) 1056
AccII CGCG 3 cut(s) 464, 890, 1139
AciI CCGC 4 cut(s) 606, 870, 1238, 1565
AclI AACGTT 4 cut(s) 12, 400, 678, 1109
AclWI GGATC 3 cut(s) 522, 821, 848
AcoI YGGCCR 2 cut(s) 155, 973
AcsI RAATTY 2 cut(s) 688, 873
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 4 cut(s) 657, 746, 1013, 1491
AfiI CCNNNNNNNGG 1 cut(s) 1442
AjiI CACGTC 1 cut(s) 104
AjnI CCWGG 3 cut(s) 718, 772, 817
AjuI GAANNNNNNNTTGG 4 cut(s) 595, 627, 1334, 1366
Alw26I GTCTC 4 cut(s) 110, 312, 556, 983
AlwI GGATC 3 cut(s) 522, 821, 848
AlwNI CAGNNNCTG 1 cut(s) 1008
AoxI GGCC 3 cut(s) 155, 770, 973
ApeKI GCWGC 3 cut(s) 466, 929, 932
ApoI RAATTY 2 cut(s) 688, 873
ArsI GACNNNNNNTTYG 4 cut(s) 350, 382, 439, 471
Asp700I GAANNNNTTC 1 cut(s) 1346
Asp718I GGTACC 1 cut(s) 1489
AspLEI GCGC 4 cut(s) 140, 466, 1139, 1141
AspS9I GGNCC 4 cut(s) 770, 815, 920, 1528
AsuHPI GGTGA 2 cut(s) 518, 1457
AvaII GGWCC 3 cut(s) 815, 920, 1528
BalI TGGCCA 1 cut(s) 157
BanI GGYRCC 1 cut(s) 1489
BauI CACGAG 1 cut(s) 574
BbsI GAAGAC 1 cut(s) 332
BbvI GCAGC 3 cut(s) 453, 916, 919
BccI CCATC 1 cut(s) 1153
BceAI ACGGC 2 cut(s) 112, 960
BciT130I CCWGG 3 cut(s) 720, 774, 819
BclI TGATCA 2 cut(s) 178, 1257
BcoDI GTCTC 4 cut(s) 110, 312, 556, 983
BfaI CTAG 3 cut(s) 693, 836, 851
BfmI CTRYAG 3 cut(s) 567, 1060, 1132
BisI GCNGC 3 cut(s) 467, 930, 933
BlsI GCNGC 3 cut(s) 468, 931, 934
Bme1390I CCNGG 3 cut(s) 720, 774, 819
Bme18I GGWCC 3 cut(s) 815, 920, 1528
BmgBI CACGTC 1 cut(s) 104
BmgT120I GGNCC 4 cut(s) 770, 815, 920, 1528
BmiI GGNNCC 3 cut(s) 286, 958, 1491
BmrFI CCNGG 3 cut(s) 720, 774, 819
BmsI GCATC 3 cut(s) 209, 1159, 1591
BpiI GAAGAC 1 cut(s) 332
BpmI CTGGAG 1 cut(s) 702
Bpu10I CCTNAGC 1 cut(s) 831
BpuEI CTTGAG 4 cut(s) 46, 886, 1450, 1541
Bsa29I ATCGAT 1 cut(s) 1331
BsaBI GATNNNNATC 1 cut(s) 513
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 5 cut(s) 84, 188, 818, 923, 1194
BsaWI WCCGGW 2 cut(s) 1502, 1549
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 1442
Bse118I RCCGGY 1 cut(s) 1525
Bse3DI GCAATG 2 cut(s) 1219, 1306
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 3 cut(s) 720, 774, 819
BseCI ATCGAT 1 cut(s) 1331
BseDI CCNNGG 5 cut(s) 84, 188, 818, 923, 1194
BseGI GGATG 1 cut(s) 389
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 1 cut(s) 1442
BseMI GCAATG 2 cut(s) 1219, 1306
BseMII CTCAG 4 cut(s) 105, 353, 542, 1256
BsePI GCGCGC 1 cut(s) 1137
BseRI GAGGAG 1 cut(s) 219
BseXI GCAGC 3 cut(s) 453, 916, 919
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 3 cut(s) 464, 890, 1139
BshFI GGCC 3 cut(s) 157, 772, 975
BshNI GGYRCC 1 cut(s) 1489
BshVI ATCGAT 1 cut(s) 1331
BsiSI CCGG 3 cut(s) 1503, 1526, 1550
BslFI GGGAC 2 cut(s) 419, 1419
BslI CCNNNNNNNGG 1 cut(s) 1442
BsmAI GTCTC 4 cut(s) 110, 312, 556, 983
BsmFI GGGAC 2 cut(s) 419, 1419
BsmI GAATGC 1 cut(s) 1170
BsnI GGCC 3 cut(s) 157, 772, 975
Bso31I GGTCTC 1 cut(s) 110
Bsp143I GATC 9 cut(s) 178, 295, 303, 514, 826, 853, 1018, 1257, 1572
Bsp68I TCGCGA 1 cut(s) 890
BspACI CCGC 4 cut(s) 606, 870, 1238, 1565
BspANI GGCC 3 cut(s) 157, 772, 975
BspCNI CTCAG 4 cut(s) 106, 352, 541, 1257
BspDI ATCGAT 1 cut(s) 1331
BspFNI CGCG 3 cut(s) 464, 890, 1139
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 3 cut(s) 286, 958, 1491
BspMAI CTGCAG 1 cut(s) 1064
BspPI GGATC 3 cut(s) 522, 821, 848
BspQI GCTCTTC 1 cut(s) 1228
BspT107I GGYRCC 1 cut(s) 1489
BspTNI GGTCTC 1 cut(s) 110
BsrBI CCGCTC 2 cut(s) 870, 1238
BsrDI GCAATG 2 cut(s) 1219, 1306
BsrFI RCCGGY 1 cut(s) 1525
BssAI RCCGGY 1 cut(s) 1525
BssECI CCNNGG 5 cut(s) 84, 188, 818, 923, 1194
BssHII GCGCGC 1 cut(s) 1137
BssMI GATC 9 cut(s) 178, 295, 303, 514, 826, 853, 1018, 1257, 1572
BssNAI GTATAC 1 cut(s) 1057
BssSI CACGAG 1 cut(s) 574
BssT1I CCWWGG 3 cut(s) 84, 188, 923
Bst1107I GTATAC 1 cut(s) 1057
Bst2BI CACGAG 1 cut(s) 574
Bst2UI CCWGG 3 cut(s) 720, 774, 819
Bst4CI ACNGT 6 cut(s) 550, 968, 1004, 1091, 1288, 1445
Bst6I CTCTTC 4 cut(s) 237, 1228, 1331, 1413
BstC8I GCNNGC 3 cut(s) 619, 1139, 1323
BstDEI CTNAG 8 cut(s) 114, 220, 339, 528, 596, 831, 1015, 1265
BstF5I GGATG 1 cut(s) 389
BstFNI CGCG 3 cut(s) 464, 890, 1139
BstHHI GCGC 4 cut(s) 140, 466, 1139, 1141
BstKTI GATC 9 cut(s) 181, 298, 306, 517, 829, 856, 1021, 1260, 1575
BstMAI GTCTC 4 cut(s) 110, 312, 556, 983
BstMBI GATC 9 cut(s) 178, 295, 303, 514, 826, 853, 1018, 1257, 1572
BstMWI GCNNNNNNNGC 7 cut(s) 251, 437, 760, 769, 956, 1147, 1176
BstNI CCWGG 3 cut(s) 720, 774, 819
BstSCI CCNGG 3 cut(s) 718, 772, 817
BstSFI CTRYAG 3 cut(s) 567, 1060, 1132
BstUI CGCG 3 cut(s) 464, 890, 1139
BstV1I GCAGC 3 cut(s) 453, 916, 919
BstV2I GAAGAC 1 cut(s) 332
BstX2I RGATCY 1 cut(s) 853
BstXI CCANNNNNNTGG 1 cut(s) 706
BstYI RGATCY 1 cut(s) 853
BstZ17I GTATAC 1 cut(s) 1057
Bsu15I ATCGAT 1 cut(s) 1331
BsuRI GGCC 3 cut(s) 157, 772, 975
BsuTUI ATCGAT 1 cut(s) 1331
BtrI CACGTC 1 cut(s) 104
BtsCI GGATG 1 cut(s) 389
BtsIMutI CAGTG 1 cut(s) 973
BtuMI TCGCGA 1 cut(s) 890
Cac8I GCNNGC 3 cut(s) 619, 1139, 1323
CaiI CAGNNNCTG 1 cut(s) 1008
CciI TCATGA 1 cut(s) 537
CfoI GCGC 4 cut(s) 140, 466, 1139, 1141
Cfr10I RCCGGY 1 cut(s) 1525
Cfr13I GGNCC 4 cut(s) 770, 815, 920, 1528
ClaI ATCGAT 1 cut(s) 1331
CpoI CGGWCCG 1 cut(s) 1528
Csp6I GTAC 4 cut(s) 656, 745, 1012, 1490
CspI CGGWCCG 1 cut(s) 1528
CviAII CATG 4 cut(s) 70, 538, 917, 1535
CviQI GTAC 4 cut(s) 656, 745, 1012, 1490
DdeI CTNAG 8 cut(s) 114, 220, 339, 528, 596, 831, 1015, 1265
DpnI GATC 9 cut(s) 180, 297, 305, 516, 828, 855, 1020, 1259, 1574
DpnII GATC 9 cut(s) 178, 295, 303, 514, 826, 853, 1018, 1257, 1572
EaeI YGGCCR 2 cut(s) 155, 973
Eam1104I CTCTTC 4 cut(s) 237, 1228, 1331, 1413
EarI CTCTTC 4 cut(s) 237, 1228, 1331, 1413
Eco130I CCWWGG 3 cut(s) 84, 188, 923
Eco31I GGTCTC 1 cut(s) 110
Eco32I GATATC 1 cut(s) 1510
Eco47I GGWCC 3 cut(s) 815, 920, 1528
Eco57I CTGAAG 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 688
EcoRII CCWGG 3 cut(s) 718, 772, 817
EcoRV GATATC 1 cut(s) 1510
EcoT14I CCWWGG 3 cut(s) 84, 188, 923
ErhI CCWWGG 3 cut(s) 84, 188, 923
FaeI CATG 4 cut(s) 73, 541, 920, 1538
FaqI GGGAC 2 cut(s) 419, 1419
FatI CATG 4 cut(s) 69, 537, 916, 1534
FauI CCCGC 2 cut(s) 599, 1231
FbaI TGATCA 2 cut(s) 178, 1257
FblI GTMKAC 1 cut(s) 1056
Fnu4HI GCNGC 3 cut(s) 467, 930, 933
FokI GGATG 1 cut(s) 396
Fsp4HI GCNGC 3 cut(s) 467, 930, 933
FspBI CTAG 3 cut(s) 693, 836, 851
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 4 cut(s) 139, 465, 1138, 1140
GluI GCNGC 3 cut(s) 467, 930, 933
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 3 cut(s) 157, 772, 975
HapII CCGG 3 cut(s) 1503, 1526, 1550
HhaI GCGC 4 cut(s) 140, 466, 1139, 1141
Hin1II CATG 4 cut(s) 73, 541, 920, 1538
Hin6I GCGC 4 cut(s) 138, 464, 1137, 1139
HinP1I GCGC 4 cut(s) 138, 464, 1137, 1139
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HindIII AAGCTT 4 cut(s) 252, 1068, 1392, 1517
HinfI GANTC 9 cut(s) 108, 328, 372, 572, 726, 793, 1249, 1328, 1432
HpaI GTTAAC 1 cut(s) 415
HpaII CCGG 3 cut(s) 1503, 1526, 1550
HphI GGTGA 2 cut(s) 518, 1457
Hpy166II GTNNAC 6 cut(s) 415, 682, 898, 1057, 1284, 1481
Hpy8I GTNNAC 6 cut(s) 415, 682, 898, 1057, 1284, 1481
Hpy99I CGWCG 5 cut(s) 108, 314, 362, 1039, 1282
HpyAV CCTTC 2 cut(s) 374, 1303
HpyCH4III ACNGT 6 cut(s) 550, 968, 1004, 1091, 1288, 1445
HpyCH4IV ACGT 9 cut(s) 12, 103, 263, 312, 400, 678, 1109, 1215, 1280
HpyCH4V TGCA 6 cut(s) 171, 292, 336, 469, 1062, 1321
HpyF10VI GCNNNNNNNGC 7 cut(s) 251, 437, 760, 769, 956, 1147, 1176
HpyF3I CTNAG 8 cut(s) 114, 220, 339, 528, 596, 831, 1015, 1265
HpySE526I ACGT 9 cut(s) 12, 103, 263, 312, 400, 678, 1109, 1215, 1280
Hsp92II CATG 4 cut(s) 73, 541, 920, 1538
HspAI GCGC 4 cut(s) 138, 464, 1137, 1139
KpnI GGTACC 1 cut(s) 1493
Ksp22I TGATCA 2 cut(s) 178, 1257
KspAI GTTAAC 1 cut(s) 415
Kzo9I GATC 9 cut(s) 178, 295, 303, 514, 826, 853, 1018, 1257, 1572
LguI GCTCTTC 1 cut(s) 1228
LmnI GCTCC 6 cut(s) 437, 721, 751, 956, 1229, 1609
Lsp1109I GCAGC 3 cut(s) 453, 916, 919
LweI GCATC 3 cut(s) 209, 1159, 1591
MaeI CTAG 3 cut(s) 693, 836, 851
MaeII ACGT 9 cut(s) 12, 103, 263, 312, 400, 678, 1109, 1215, 1280
MaeIII GTNAC 4 cut(s) 52, 194, 445, 1445
MalI GATC 9 cut(s) 180, 297, 305, 516, 828, 855, 1020, 1259, 1574
MbiI CCGCTC 2 cut(s) 870, 1238
MboI GATC 9 cut(s) 178, 295, 303, 514, 826, 853, 1018, 1257, 1572
MboII GAAGA 9 cut(s) 224, 337, 415, 746, 749, 1244, 1245, 1348, 1430
MflI RGATCY 1 cut(s) 853
MlsI TGGCCA 1 cut(s) 157
MluCI AATT 6 cut(s) 211, 688, 859, 873, 881, 906
MluNI TGGCCA 1 cut(s) 157
MlyI GAGTC 4 cut(s) 322, 566, 787, 1441
MmeI TCCRAC 4 cut(s) 295, 922, 1057, 1363
MnlI CCTC 7 cut(s) 57, 240, 261, 497, 1073, 1204, 1332
Mox20I TGGCCA 1 cut(s) 157
MroXI GAANNNNTTC 1 cut(s) 1346
MscI TGGCCA 1 cut(s) 157
MseI TTAA 3 cut(s) 414, 963, 999
Msp20I TGGCCA 1 cut(s) 157
MspA1I CMGCKG 1 cut(s) 1567
MspI CCGG 3 cut(s) 1503, 1526, 1550
MspR9I CCNGG 3 cut(s) 720, 774, 819
Mva1269I GAATGC 1 cut(s) 1170
MvaI CCWGG 3 cut(s) 720, 774, 819
MvnI CGCG 3 cut(s) 464, 890, 1139
MwoI GCNNNNNNNGC 7 cut(s) 251, 437, 760, 769, 956, 1147, 1176
NdeII GATC 9 cut(s) 178, 295, 303, 514, 826, 853, 1018, 1257, 1572
NlaIII CATG 4 cut(s) 73, 541, 920, 1538
NlaIV GGNNCC 3 cut(s) 286, 958, 1491
NmeAIII GCCGAG 1 cut(s) 1155
NmuCI GTSAC 2 cut(s) 445, 1445
NruI TCGCGA 1 cut(s) 890
NsbI TGCGCA 1 cut(s) 139
PagI TCATGA 1 cut(s) 537
PauI GCGCGC 1 cut(s) 1137
PciSI GCTCTTC 1 cut(s) 1228
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 1170
PdmI GAANNNNTTC 1 cut(s) 1346
PfeI GAWTC 5 cut(s) 108, 372, 726, 1249, 1328
PkrI GCNGC 3 cut(s) 468, 931, 934
PleI GAGTC 4 cut(s) 322, 566, 787, 1440
PpsI GAGTC 4 cut(s) 322, 566, 787, 1440
Psp1406I AACGTT 4 cut(s) 12, 400, 678, 1109
Psp6I CCWGG 3 cut(s) 718, 772, 817
PspGI CCWGG 3 cut(s) 718, 772, 817
PspN4I GGNNCC 3 cut(s) 286, 958, 1491
PspPI GGNCC 4 cut(s) 770, 815, 920, 1528
PstI CTGCAG 1 cut(s) 1064
PstNI CAGNNNCTG 1 cut(s) 1008
PsuI RGATCY 1 cut(s) 853
PteI GCGCGC 1 cut(s) 1137
RruI TCGCGA 1 cut(s) 890
RsaI GTAC 4 cut(s) 657, 746, 1013, 1491
RsaNI GTAC 4 cut(s) 656, 745, 1012, 1490
Rsr2I CGGWCCG 1 cut(s) 1528
RsrII CGGWCCG 1 cut(s) 1528
SapI GCTCTTC 1 cut(s) 1228
SaqAI TTAA 3 cut(s) 414, 963, 999
SatI GCNGC 3 cut(s) 467, 930, 933
Sau3AI GATC 9 cut(s) 178, 295, 303, 514, 826, 853, 1018, 1257, 1572
Sau96I GGNCC 4 cut(s) 770, 815, 920, 1528
SchI GAGTC 4 cut(s) 322, 566, 787, 1441
ScrFI CCNGG 3 cut(s) 720, 774, 819
SfaNI GCATC 3 cut(s) 209, 1159, 1591
SfcI CTRYAG 3 cut(s) 567, 1060, 1132
SinI GGWCC 3 cut(s) 815, 920, 1528
SmlI CTYRAG 4 cut(s) 25, 865, 1429, 1520
SmoI CTYRAG 4 cut(s) 25, 865, 1429, 1520
Sse9I AATT 6 cut(s) 211, 688, 859, 873, 881, 906
SsiI CCGC 4 cut(s) 606, 870, 1238, 1565
SspMI CTAG 3 cut(s) 693, 836, 851
StyD4I CCNGG 3 cut(s) 718, 772, 817
StyI CCWWGG 3 cut(s) 84, 188, 923
TaaI ACNGT 6 cut(s) 550, 968, 1004, 1091, 1288, 1445
TaiI ACGT 9 cut(s) 15, 106, 266, 315, 403, 681, 1112, 1218, 1283
TaqI TCGA 4 cut(s) 106, 1037, 1331, 1571
TaqII GACCGA 1 cut(s) 134
TasI AATT 6 cut(s) 211, 688, 859, 873, 881, 906
TatI WGTACW 1 cut(s) 655
TfiI GAWTC 5 cut(s) 108, 372, 726, 1249, 1328
Tru1I TTAA 3 cut(s) 414, 963, 999
Tru9I TTAA 3 cut(s) 414, 963, 999
TscAI CASTG 1 cut(s) 973
TseFI GTSAC 2 cut(s) 445, 1445
TseI GCWGC 3 cut(s) 466, 929, 932
Tsp45I GTSAC 2 cut(s) 445, 1445
TspDTI ATGAA 7 cut(s) 410, 526, 554, 985, 1208, 1349, 1570
TspGWI ACGGA 4 cut(s) 375, 504, 1416, 1520
TspRI CASTG 1 cut(s) 973
VpaK11BI GGWCC 3 cut(s) 815, 920, 1528
XapI RAATTY 2 cut(s) 688, 873
XbaI TCTAGA 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 1056
XmnI GAANNNNTTC 1 cut(s) 1346
XspI CTAG 3 cut(s) 693, 836, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.