Rh4AG363500

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004486
Physical Location & Seq
Forward (+)
67212039 .. 67214882
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG363500.1

Sequence Viewer

Length: 927 bp
ATGAAAAAGCTCAAACAACTTGTGACAGAGTTGTTCCCAGATGGTAAACAGCCAAAGGTTTTAGGCCCCGGCGGCTTTTATGAGAAAAAGTGGTTCAATGACTTCCTGCAGGCCACCGGACCCGGTGTCCTAGACGGTGTGACTCATCACACTTACGACCTTGGTGCTGGTGTTGATCCAACCCTTATAACCAAGGTTCAAGACCCATTTTACCTGGACCATTCATCTCAGACATATAATGATGTGGCAAACTCAGTTAAACAGTTTGCCCCATGGACAGACCCTTGGGTTGGTGAGGCTGGTGGAGCTTACAACAGTGGTGGCAAAGATGTTTCACATACCTTTGCTAATGGCTTTTGGTATTTGGACCAACTTGGTATGACAGCAAGCTACAGTCACAAGGTTTACTGCAGACAAGCCTTGATTGGAGGGAACTATGCCCTACTTAATACCACATCTTTCATCCCTAATCCTGACTACTATGGTGCACTCCTATGGCATAGACTGATGGGAAATAAAGTGCTCTCTACCGTTCATTATGGCTCTCCCTTCTTGCGTGCATATACTCATTGTTCAAAGAAAAAGCCTGGGGTCACTCTGCTTTTGATCAACATGTCCAACTCTACTACCTTCGATGTTGAAGTTTTCCCCGAGTTCAATCTGCATCCTTGGCAAAGATATGAGGTACAATCGGACACAAAATCAGCTGGTGCACCAATGAGAGAAGAGTATCATTTGACACCAGAAGGTGGCAATATCCAAAGTGATGTGTTGCTACTGAATGGGACTCCATTGAAGCTCACAGAGTCATCCGACATTCCTGAACTGCAACCAAAACTAGTGGATCCTACCTCTGCAGTCAGTGTTGCACCACAGTCGTTCGTCTTTGTATCTATCAAGGACTTCCATGCCCCTGCATGCGCTTAG

Protein Analysis

308

Amino Acids

34.07

Weight (kDa)

6.1

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 2 - 126 7.8e-50 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AccB7I CCANNNNNTGG 1 cut(s) 749
AciI CCGC 1 cut(s) 72
AclWI GGATC 3 cut(s) 170, 839, 852
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 2 cut(s) 290, 749
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 6 cut(s) 97, 200, 576, 641, 658, 796
AhdI GACNNNNNGTC 1 cut(s) 125
AhlI ACTAGT 1 cut(s) 838
AjnI CCWGG 2 cut(s) 213, 586
AluBI AGCT 5 cut(s) 10, 308, 390, 707, 799
AluI AGCT 5 cut(s) 10, 308, 390, 707, 799
Alw21I GWGCWC 3 cut(s) 490, 525, 715
Alw44I GTGCAC 2 cut(s) 486, 711
AlwI GGATC 3 cut(s) 170, 839, 852
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 2 cut(s) 64, 111
ApaLI GTGCAC 2 cut(s) 486, 711
AspLEI GCGC 1 cut(s) 923
AspS9I GGNCC 4 cut(s) 65, 119, 217, 367
AsuC2I CCSGG 2 cut(s) 69, 123
AsuHPI GGTGA 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 3 cut(s) 119, 217, 367
BaeGI GKGCMC 2 cut(s) 490, 715
BamHI GGATCC 1 cut(s) 844
Bbv12I GWGCWC 3 cut(s) 490, 525, 715
BccI CCATC 2 cut(s) 35, 502
BcgI CGANNNNNNTGC 4 cut(s) 146, 180, 858, 892
BciT130I CCWGG 2 cut(s) 215, 588
BclI TGATCA 1 cut(s) 606
BcnI CCSGG 2 cut(s) 69, 123
BcuI ACTAGT 1 cut(s) 838
BfaI CTAG 2 cut(s) 131, 839
BfmI CTRYAG 4 cut(s) 107, 391, 409, 855
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 1 cut(s) 73
BlsI GCNGC 1 cut(s) 74
Bme1390I CCNGG 4 cut(s) 69, 123, 215, 588
Bme18I GGWCC 3 cut(s) 119, 217, 367
BmeRI GACNNNNNGTC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 4 cut(s) 65, 119, 217, 367
BmiI GGNNCC 3 cut(s) 67, 121, 846
BmrFI CCNGG 4 cut(s) 69, 123, 215, 588
BmsI GCATC 1 cut(s) 673
BpuMI CCSGG 2 cut(s) 69, 123
BsaJI CCNNGG 7 cut(s) 67, 160, 192, 272, 284, 587, 668
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 749
BseBI CCWGG 2 cut(s) 215, 588
BseDI CCNNGG 7 cut(s) 67, 160, 192, 272, 284, 587, 668
BseGI GGATG 3 cut(s) 462, 664, 809
BseLI CCNNNNNNNGG 2 cut(s) 290, 749
BseMII CTCAG 2 cut(s) 242, 267
BseSI GKGCMC 2 cut(s) 490, 715
BshFI GGCC 2 cut(s) 66, 113
BsiHKAI GWGCWC 3 cut(s) 490, 525, 715
BsiHKCI CYCGRG 1 cut(s) 650
BsiSI CCGG 3 cut(s) 69, 117, 123
BslFI GGGAC 1 cut(s) 799
BslI CCNNNNNNNGG 2 cut(s) 290, 749
BsmFI GGGAC 1 cut(s) 799
BsnI GGCC 2 cut(s) 66, 113
BsoBI CYCGRG 1 cut(s) 650
Bsp1286I GDGCHC 3 cut(s) 490, 525, 715
Bsp143I GATC 3 cut(s) 175, 606, 844
Bsp19I CCATGG 1 cut(s) 272
BspACI CCGC 1 cut(s) 72
BspANI GGCC 2 cut(s) 66, 113
BspCNI CTCAG 2 cut(s) 241, 266
BspLI GGNNCC 3 cut(s) 67, 121, 846
BspMAI CTGCAG 3 cut(s) 111, 413, 859
BspPI GGATC 3 cut(s) 170, 839, 852
BssECI CCNNGG 7 cut(s) 67, 160, 192, 272, 284, 587, 668
BssMI GATC 3 cut(s) 175, 606, 844
BssT1I CCWWGG 5 cut(s) 160, 192, 272, 284, 668
Bst2UI CCWGG 2 cut(s) 215, 588
Bst4CI ACNGT 6 cut(s) 137, 264, 317, 395, 532, 876
Bst6I CTCTTC 1 cut(s) 720
BstC8I GCNNGC 4 cut(s) 111, 388, 558, 919
BstDEI CTNAG 3 cut(s) 228, 253, 924
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 3 cut(s) 462, 664, 809
BstHHI GCGC 1 cut(s) 923
BstKTI GATC 3 cut(s) 178, 609, 847
BstMBI GATC 3 cut(s) 175, 606, 844
BstMWI GCNNNNNNNGC 3 cut(s) 72, 305, 670
BstNI CCWGG 2 cut(s) 215, 588
BstNSI RCATGY 2 cut(s) 616, 921
BstSCI CCNGG 4 cut(s) 67, 121, 213, 586
BstSFI CTRYAG 4 cut(s) 107, 391, 409, 855
BstSLI GKGCMC 2 cut(s) 490, 715
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
BsuRI GGCC 2 cut(s) 66, 113
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 3 cut(s) 462, 664, 809
BtsIMutI CAGTG 2 cut(s) 322, 868
Cac8I GCNNGC 4 cut(s) 111, 388, 558, 919
CfoI GCGC 1 cut(s) 923
Cfr13I GGNCC 4 cut(s) 65, 119, 217, 367
Csp6I GTAC 1 cut(s) 686
CspCI CAANNNNNGTGG 4 cut(s) 301, 336, 822, 857
CviAII CATG 4 cut(s) 273, 613, 908, 918
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 3 cut(s) 228, 253, 924
DpnI GATC 3 cut(s) 177, 608, 846
DpnII GATC 3 cut(s) 175, 606, 844
DriI GACNNNNNGTC 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 720
Eam1105I GACNNNNNGTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 720
Eco130I CCWWGG 5 cut(s) 160, 192, 272, 284, 668
Eco47I GGWCC 3 cut(s) 119, 217, 367
Eco88I CYCGRG 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 213, 586
EcoT14I CCWWGG 5 cut(s) 160, 192, 272, 284, 668
ErhI CCWWGG 5 cut(s) 160, 192, 272, 284, 668
FaeI CATG 4 cut(s) 276, 616, 911, 921
FaqI GGGAC 1 cut(s) 799
FatI CATG 4 cut(s) 272, 612, 907, 917
FbaI TGATCA 1 cut(s) 606
Fnu4HI GCNGC 1 cut(s) 73
FokI GGATG 3 cut(s) 449, 651, 796
Fsp4HI GCNGC 1 cut(s) 73
FspBI CTAG 2 cut(s) 131, 839
GlaI GCGC 1 cut(s) 922
GluI GCNGC 1 cut(s) 73
HaeIII GGCC 2 cut(s) 66, 113
HapII CCGG 3 cut(s) 69, 117, 123
HhaI GCGC 1 cut(s) 923
Hin1II CATG 4 cut(s) 276, 616, 911, 921
Hin6I GCGC 1 cut(s) 921
HinP1I GCGC 1 cut(s) 921
HinfI GANTC 3 cut(s) 142, 787, 806
HpaII CCGG 3 cut(s) 69, 117, 123
HphI GGTGA 1 cut(s) 305
Hpy166II GTNNAC 4 cut(s) 47, 406, 488, 713
Hpy188I TCNGA 3 cut(s) 231, 694, 814
Hpy188III TCNNGA 3 cut(s) 200, 473, 821
Hpy8I GTNNAC 4 cut(s) 47, 406, 488, 713
HpyAV CCTTC 3 cut(s) 559, 640, 740
HpyCH4III ACNGT 6 cut(s) 137, 264, 317, 395, 532, 876
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 305, 670
HpyF3I CTNAG 3 cut(s) 228, 253, 924
Hsp92II CATG 4 cut(s) 276, 616, 911, 921
HspAI GCGC 1 cut(s) 921
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 3 cut(s) 175, 606, 844
LmnI GCTCC 1 cut(s) 305
LweI GCATC 1 cut(s) 673
MaeI CTAG 2 cut(s) 131, 839
MaeIII GTNAC 4 cut(s) 22, 139, 395, 592
MalI GATC 3 cut(s) 177, 608, 846
MboI GATC 3 cut(s) 175, 606, 844
MboII GAAGA 1 cut(s) 737
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 3 cut(s) 490, 525, 715
MlyI GAGTC 3 cut(s) 136, 781, 815
MmeI TCCRAC 3 cut(s) 203, 642, 837
MnlI CCTC 4 cut(s) 289, 422, 676, 862
MseI TTAA 2 cut(s) 258, 447
MslI CAYNNNNRTG 1 cut(s) 493
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 3 cut(s) 69, 117, 123
MspR9I CCNGG 4 cut(s) 69, 123, 215, 588
MvaI CCWGG 2 cut(s) 215, 588
MwoI GCNNNNNNNGC 3 cut(s) 72, 305, 670
NciI CCSGG 2 cut(s) 69, 123
NcoI CCATGG 1 cut(s) 272
NdeII GATC 3 cut(s) 175, 606, 844
NlaIII CATG 4 cut(s) 276, 616, 911, 921
NlaIV GGNNCC 3 cut(s) 67, 121, 846
NmuCI GTSAC 4 cut(s) 22, 139, 395, 592
NspI RCATGY 2 cut(s) 616, 921
PaeI GCATGC 1 cut(s) 921
PciI ACATGT 1 cut(s) 612
PflMI CCANNNNNTGG 1 cut(s) 749
PkrI GCNGC 1 cut(s) 74
PleI GAGTC 3 cut(s) 136, 781, 814
PpsI GAGTC 3 cut(s) 136, 781, 814
PscI ACATGT 1 cut(s) 612
PsiI TTATAA 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 213, 586
PspGI CCWGG 2 cut(s) 213, 586
PspN4I GGNNCC 3 cut(s) 67, 121, 846
PspPI GGNCC 4 cut(s) 65, 119, 217, 367
PstI CTGCAG 3 cut(s) 111, 413, 859
PsuI RGATCY 1 cut(s) 844
PvuII CAGCTG 1 cut(s) 707
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
RseI CAYNNNNRTG 1 cut(s) 493
SaqAI TTAA 2 cut(s) 258, 447
SatI GCNGC 1 cut(s) 73
Sau3AI GATC 3 cut(s) 175, 606, 844
Sau96I GGNCC 4 cut(s) 65, 119, 217, 367
SbfI CCTGCAGG 1 cut(s) 111
SchI GAGTC 3 cut(s) 136, 781, 815
ScrFI CCNGG 4 cut(s) 69, 123, 215, 588
SdaI CCTGCAGG 1 cut(s) 111
SduI GDGCHC 3 cut(s) 490, 525, 715
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 4 cut(s) 107, 391, 409, 855
SinI GGWCC 3 cut(s) 119, 217, 367
SmiMI CAYNNNNRTG 1 cut(s) 493
SpeI ACTAGT 1 cut(s) 838
SphI GCATGC 1 cut(s) 921
Sse8387I CCTGCAGG 1 cut(s) 111
SsiI CCGC 1 cut(s) 72
SspMI CTAG 2 cut(s) 131, 839
StyD4I CCNGG 4 cut(s) 67, 121, 213, 586
StyI CCWWGG 5 cut(s) 160, 192, 272, 284, 668
TaaI ACNGT 6 cut(s) 137, 264, 317, 395, 532, 876
TaqI TCGA 1 cut(s) 633
TauI GCSGC 1 cut(s) 75
Tru1I TTAA 2 cut(s) 258, 447
Tru9I TTAA 2 cut(s) 258, 447
TscAI CASTG 2 cut(s) 322, 868
TseFI GTSAC 4 cut(s) 22, 139, 395, 592
Tsp45I GTSAC 4 cut(s) 22, 139, 395, 592
TspDTI ATGAA 4 cut(s) 17, 213, 451, 524
TspRI CASTG 2 cut(s) 322, 868
Van91I CCANNNNNTGG 1 cut(s) 749
VneI GTGCAC 2 cut(s) 486, 711
VpaK11BI GGWCC 3 cut(s) 119, 217, 367
XceI RCATGY 2 cut(s) 616, 921
XspI CTAG 2 cut(s) 131, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.