Rh5DG336800

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004486
Physical Location & Seq
Forward (+)
45236946 .. 45238018
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG336800.1

Sequence Viewer

Length: 786 bp
ATGGGGTCGAGTGCTATAGCATGTTGCATTCTGTTGTCATTGTTCTCAGTAGCTTTAGCTGAATATGTCGATGTCAGGGTTCGAGGAGTGGTTTCGATAGCTAGCACAGATGACAATTTCATATGTGCAACCCTGGATTGGTGGCCAACTGATAAATGTGATTACAATCAATGTCCTTGGGGAAAAGCTGGAATTTTTAATCTGGATTTGAAGAACAAGACCTTGATCAATGCAGTAAAGGCATTCAATACAATAAGGATCAGAATTGGACGTTCATTGCAAGACCAAATTATTTATAATTTTGGTTATGGAGTGAAAGACTGCAACCAAATCGTGAAAGATGGTTCTGGGCCTTTCTTGTTCCGTGGCGGTTGTCCTCAGCCGAAAAGATGGGATGAGATCCATGAGTTCTTCAACCAAACACAAGCCAAACTCTTGTTTGGATTGAATGCCCTTCATGGGAAGCAAGCGGACCTTAAAGACAAGATTCTTTGGGTTGGTGATTGGGACGCAAAAAACGCTCGTGATCTCATGGAGTACACCATCTCAAAGGGTTACCCTGCTGAGTCATATGAGCTTGGAAACGAGTTATGTGGGAGTGGGGTAGGTGCAAGAATAGAAGCTGAACAATATGCCAAAGACATGAAAAAGCTCAAACAACTTGTGACAGAGTTGTTCCCAGATGGTAAACAGCCAAAGGTTTTAGGCCCCGGCGGCTCTGAACCCTTATACTCTTTTTTAGTTGTCTTAATCACCTTGTTTAAGTTGCACTCGTGTGGTTCCTAA

Protein Analysis

261

Amino Acids

29.03

Weight (kDa)

7.48

Isoelectric Point (pI)

26.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 24 - 239 3.4e-88 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
AciI CCGC 3 cut(s) 369, 470, 714
AclWI GGATC 2 cut(s) 266, 394
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 1 cut(s) 192
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 2 cut(s) 138, 459
AgsI TTSAA 4 cut(s) 211, 247, 415, 448
AjnI CCWGG 1 cut(s) 132
AleI CACNNNNGTG 1 cut(s) 774
AluBI AGCT 7 cut(s) 53, 59, 101, 188, 577, 623, 652
AluI AGCT 7 cut(s) 53, 59, 101, 188, 577, 623, 652
AlwI GGATC 2 cut(s) 266, 394
AoxI GGCC 3 cut(s) 143, 350, 706
ApoI RAATTY 1 cut(s) 192
AspS9I GGNCC 3 cut(s) 350, 472, 707
AsuC2I CCSGG 1 cut(s) 711
AsuHPI GGTGA 2 cut(s) 512, 745
AsuNHI GCTAGC 1 cut(s) 101
AvaII GGWCC 1 cut(s) 472
BalI TGGCCA 1 cut(s) 145
BauI CACGAG 2 cut(s) 522, 772
BbvCI CCTCAGC 1 cut(s) 378
BccI CCATC 4 cut(s) 335, 384, 551, 677
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 1 cut(s) 134
BclI TGATCA 1 cut(s) 225
BcnI CCSGG 1 cut(s) 711
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 15
BglI GCCNNNNNGGC 1 cut(s) 714
BisI GCNGC 1 cut(s) 715
BlsI GCNGC 1 cut(s) 716
Bme1390I CCNGG 2 cut(s) 134, 711
Bme18I GGWCC 1 cut(s) 472
BmgT120I GGNCC 3 cut(s) 350, 472, 707
BmiI GGNNCC 2 cut(s) 709, 781
BmrFI CCNGG 2 cut(s) 134, 711
BmtI GCTAGC 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 165
BsaJI CCNNGG 4 cut(s) 132, 176, 364, 709
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 459
Bse3DI GCAATG 1 cut(s) 275
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 4 cut(s) 132, 176, 364, 709
BseGI GGATG 1 cut(s) 400
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 138, 459
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 3 cut(s) 60, 392, 555
BseRI GAGGAG 1 cut(s) 99
BshFI GGCC 3 cut(s) 145, 352, 708
BsiSI CCGG 1 cut(s) 711
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 2 cut(s) 138, 459
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 3 cut(s) 27, 242, 454
BsnI GGCC 3 cut(s) 145, 352, 708
Bsp143I GATC 4 cut(s) 225, 258, 399, 526
BspACI CCGC 3 cut(s) 369, 470, 714
BspANI GGCC 3 cut(s) 145, 352, 708
BspCNI CTCAG 3 cut(s) 59, 391, 556
BspLI GGNNCC 2 cut(s) 709, 781
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 2 cut(s) 266, 394
BsrDI GCAATG 1 cut(s) 275
BssECI CCNNGG 4 cut(s) 132, 176, 364, 709
BssMI GATC 4 cut(s) 225, 258, 399, 526
BssSI CACGAG 2 cut(s) 522, 772
BssT1I CCWWGG 1 cut(s) 176
Bst2BI CACGAG 2 cut(s) 522, 772
Bst2UI CCWGG 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 103, 468
BstDEI CTNAG 3 cut(s) 46, 378, 564
BstDSI CCRYGG 1 cut(s) 364
BstEII GGTNACC 1 cut(s) 554
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 4 cut(s) 228, 261, 402, 529
BstMBI GATC 4 cut(s) 225, 258, 399, 526
BstMWI GCNNNNNNNGC 3 cut(s) 239, 518, 714
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 24
BstPI GGTNACC 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 132, 709
BstSFI CTRYAG 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 399
BstYI RGATCY 1 cut(s) 399
BsuRI GGCC 3 cut(s) 145, 352, 708
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 103, 468
Cfr13I GGNCC 3 cut(s) 350, 472, 707
CseI GACGC 1 cut(s) 518
Csp6I GTAC 1 cut(s) 538
CviAII CATG 5 cut(s) 21, 404, 458, 532, 643
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 3 cut(s) 46, 378, 564
DpnI GATC 4 cut(s) 227, 260, 401, 528
DpnII GATC 4 cut(s) 225, 258, 399, 526
EaeI YGGCCR 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 472
Eco91I GGTNACC 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 707
EcoO65I GGTNACC 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 132
EcoT14I CCWWGG 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 5 cut(s) 24, 407, 461, 535, 646
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 1 cut(s) 521
FatI CATG 5 cut(s) 20, 403, 457, 531, 642
FauNDI CATATG 2 cut(s) 122, 571
FbaI TGATCA 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 715
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 1 cut(s) 715
FspBI CTAG 1 cut(s) 102
GluI GCNGC 1 cut(s) 715
HaeIII GGCC 3 cut(s) 145, 352, 708
HapII CCGG 1 cut(s) 711
HgaI GACGC 1 cut(s) 518
Hin1II CATG 5 cut(s) 24, 407, 461, 535, 646
HinfI GANTC 2 cut(s) 487, 566
HpaII CCGG 1 cut(s) 711
HphI GGTGA 2 cut(s) 512, 745
Hpy166II GTNNAC 2 cut(s) 540, 689
Hpy188I TCNGA 2 cut(s) 263, 721
Hpy188III TCNNGA 3 cut(s) 203, 334, 524
Hpy8I GTNNAC 2 cut(s) 540, 689
HpyAV CCTTC 1 cut(s) 464
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 7 cut(s) 27, 128, 233, 280, 324, 611, 769
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 518, 714
HpyF3I CTNAG 3 cut(s) 46, 378, 564
HpySE526I ACGT 1 cut(s) 271
Hsp92II CATG 5 cut(s) 24, 407, 461, 535, 646
Ksp22I TGATCA 1 cut(s) 225
Kzo9I GATC 4 cut(s) 225, 258, 399, 526
LpnPI CCDG 9 cut(s) 61, 119, 146, 174, 188, 333, 573, 693, 724
MaeI CTAG 1 cut(s) 102
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 554, 664
MalI GATC 4 cut(s) 227, 260, 401, 528
MboI GATC 4 cut(s) 225, 258, 399, 526
MboII GAAGA 2 cut(s) 223, 403
MflI RGATCY 1 cut(s) 399
MlsI TGGCCA 1 cut(s) 145
MluCI AATT 5 cut(s) 115, 192, 264, 288, 298
MluNI TGGCCA 1 cut(s) 145
MlyI GAGTC 1 cut(s) 575
MnlI CCTC 2 cut(s) 77, 387
Mox20I TGGCCA 1 cut(s) 145
MscI TGGCCA 1 cut(s) 145
MseI TTAA 4 cut(s) 198, 477, 749, 762
MslI CAYNNNNRTG 1 cut(s) 774
Msp20I TGGCCA 1 cut(s) 145
MspI CCGG 1 cut(s) 711
MspR9I CCNGG 2 cut(s) 134, 711
Mva1269I GAATGC 3 cut(s) 27, 242, 454
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 3 cut(s) 239, 518, 714
NciI CCSGG 1 cut(s) 711
NdeI CATATG 2 cut(s) 122, 571
NdeII GATC 4 cut(s) 225, 258, 399, 526
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 5 cut(s) 24, 407, 461, 535, 646
NlaIV GGNNCC 2 cut(s) 709, 781
NmuCI GTSAC 1 cut(s) 664
NspI RCATGY 1 cut(s) 24
OliI CACNNNNGTG 1 cut(s) 774
PctI GAATGC 3 cut(s) 27, 242, 454
PfeI GAWTC 1 cut(s) 487
PkrI GCNGC 1 cut(s) 716
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PsiI TTATAA 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 132
PspEI GGTNACC 1 cut(s) 554
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 2 cut(s) 709, 781
PspPI GGNCC 3 cut(s) 350, 472, 707
PsuI RGATCY 1 cut(s) 399
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 1 cut(s) 774
SaqAI TTAA 4 cut(s) 198, 477, 749, 762
SatI GCNGC 1 cut(s) 715
Sau3AI GATC 4 cut(s) 225, 258, 399, 526
Sau96I GGNCC 3 cut(s) 350, 472, 707
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 2 cut(s) 134, 711
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 1 cut(s) 472
SmiMI CAYNNNNRTG 1 cut(s) 774
Sse9I AATT 5 cut(s) 115, 192, 264, 288, 298
SsiI CCGC 3 cut(s) 369, 470, 714
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 2 cut(s) 132, 709
StyI CCWWGG 1 cut(s) 176
TaiI ACGT 1 cut(s) 274
TaqI TCGA 4 cut(s) 8, 69, 82, 95
TasI AATT 5 cut(s) 115, 192, 264, 288, 298
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 717
TfiI GAWTC 1 cut(s) 487
Tru1I TTAA 4 cut(s) 198, 477, 749, 762
Tru9I TTAA 4 cut(s) 198, 477, 749, 762
TseFI GTSAC 1 cut(s) 664
Tsp45I GTSAC 1 cut(s) 664
TspDTI ATGAA 4 cut(s) 109, 264, 446, 659
TspGWI ACGGA 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 472
XapI RAATTY 1 cut(s) 192
XceI RCATGY 1 cut(s) 24
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.