Rh1AG210500

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004486
Physical Location & Seq
Forward (+)
40023785 .. 40025215
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG210500.1

Sequence Viewer

Length: 906 bp
ATGGGGTCGAGAGCTATTGCATGTTGCATTCTGTTGTCATTGTTCTCAGTAGCTTTAGCTGAATATGTCGATGTCAGGGTTCGAGGAGTGGTTTCGATAGCTAGCACAGATGACAATTTCATATGTGCAACCCTGGATTGGTGGCCAACTGATAAATGTGATTACAATCAATGTCCTTGGGGAAAAGCTGGAATTTTTAATCTGGATTTGAAGAACAAGACCTTGATCAATGCAGTAAAGGCATTCAATACAATAAGGATCAGAATTGGAGGTTCATTGCAAGACCAAATTATTTATAATTTTGGTTATGGAGTGAAAGACTGCAACCAAATCGTGAAAGATGGTTCTGGGCCTTTCTTGTTCCGTGGCGGTTGTCTTCAGCCGAAAAGATGGGATGAGATCCATGAGTTCTTCAACCAAACACAAGCCAAACTCTTGTTTGGATTGAATGCCCTTCATGGGAAGAAAGCGGACCCTAAAGACAAGATTTTTTGGGTTGGTGATTGGGACGCAAAAAACGCTCGTGATCTCATGGAGTACACCATCTCAAAGGGTTACCCTGTTGAGTCATATGAGCTTGGAAACGAGTTATGTGGGAGTGGGGTAGGTGCAAGAATAGAAGCTGAACAATATGCCAAAGACATGAAAAAGCTCAAACAACTTGTGACAGAGTTGTTCCCAGATGGTAAACAGCCAAAGGTTTTAGGCCCCGGCGGCTTTTATGAGAAAAAATGGTTCAATGACTTCCTGCAGGCCACCGGACCCGGTGTCCTAGATGGTGTGACTCATCACACTTACGACCTTGGTGCTGGTGTGGATCCAACCCTTATAACCAAGGTTCAAGACCCATTTTACCTGGACCATTCATCTCAGACATATAATGATGTGGCAAACTCAGTTAAATAG

Protein Analysis

301

Amino Acids

33.57

Weight (kDa)

6.22

Isoelectric Point (pI)

24.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 24 - 301 3.4e-118 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 297, 830
AciI CCGC 3 cut(s) 369, 470, 714
AclWI GGATC 4 cut(s) 266, 394, 812, 825
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 2 cut(s) 138, 459
AgsI TTSAA 6 cut(s) 211, 247, 415, 448, 739, 842
AhdI GACNNNNNGTC 1 cut(s) 767
AjnI CCWGG 2 cut(s) 132, 855
AluBI AGCT 8 cut(s) 14, 53, 59, 101, 188, 577, 623, 652
AluI AGCT 8 cut(s) 14, 53, 59, 101, 188, 577, 623, 652
AlwI GGATC 4 cut(s) 266, 394, 812, 825
AoxI GGCC 4 cut(s) 143, 350, 706, 753
ApoI RAATTY 1 cut(s) 192
AspS9I GGNCC 5 cut(s) 350, 472, 707, 761, 859
AsuC2I CCSGG 2 cut(s) 711, 765
AsuHPI GGTGA 1 cut(s) 512
AsuNHI GCTAGC 1 cut(s) 101
AvaII GGWCC 3 cut(s) 472, 761, 859
BalI TGGCCA 1 cut(s) 145
BamHI GGATCC 1 cut(s) 817
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 368
BccI CCATC 5 cut(s) 335, 384, 551, 677, 770
BcgI CGANNNNNNTGC 4 cut(s) 313, 347, 788, 822
BciT130I CCWGG 2 cut(s) 134, 857
BclI TGATCA 1 cut(s) 225
BcnI CCSGG 2 cut(s) 711, 765
BfaI CTAG 2 cut(s) 102, 773
BfmI CTRYAG 1 cut(s) 749
BglI GCCNNNNNGGC 1 cut(s) 714
BisI GCNGC 1 cut(s) 715
BlsI GCNGC 1 cut(s) 716
Bme1390I CCNGG 4 cut(s) 134, 711, 765, 857
Bme18I GGWCC 3 cut(s) 472, 761, 859
BmeRI GACNNNNNGTC 1 cut(s) 767
BmgT120I GGNCC 5 cut(s) 350, 472, 707, 761, 859
BmiI GGNNCC 4 cut(s) 474, 709, 763, 819
BmrFI CCNGG 4 cut(s) 134, 711, 765, 857
BmtI GCTAGC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 368
BpuMI CCSGG 2 cut(s) 711, 765
BsaBI GATNNNNATC 1 cut(s) 165
BsaJI CCNNGG 6 cut(s) 132, 176, 364, 709, 802, 834
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 459
Bse3DI GCAATG 1 cut(s) 275
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 2 cut(s) 134, 857
BseDI CCNNGG 6 cut(s) 132, 176, 364, 709, 802, 834
BseGI GGATG 1 cut(s) 400
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 138, 459
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 2 cut(s) 60, 884
BseRI GAGGAG 1 cut(s) 99
BshFI GGCC 4 cut(s) 145, 352, 708, 755
BsiSI CCGG 3 cut(s) 711, 759, 765
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 2 cut(s) 138, 459
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 3 cut(s) 27, 242, 454
BsnI GGCC 4 cut(s) 145, 352, 708, 755
Bsp143I GATC 5 cut(s) 225, 258, 399, 526, 817
BspACI CCGC 3 cut(s) 369, 470, 714
BspANI GGCC 4 cut(s) 145, 352, 708, 755
BspCNI CTCAG 2 cut(s) 59, 883
BspLI GGNNCC 4 cut(s) 474, 709, 763, 819
BspMAI CTGCAG 1 cut(s) 753
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 4 cut(s) 266, 394, 812, 825
BsrDI GCAATG 1 cut(s) 275
BssECI CCNNGG 6 cut(s) 132, 176, 364, 709, 802, 834
BssMI GATC 5 cut(s) 225, 258, 399, 526, 817
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 3 cut(s) 176, 802, 834
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 134, 857
BstC8I GCNNGC 2 cut(s) 103, 753
BstDEI CTNAG 3 cut(s) 46, 870, 895
BstDSI CCRYGG 1 cut(s) 364
BstEII GGTNACC 1 cut(s) 554
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 5 cut(s) 228, 261, 402, 529, 820
BstMBI GATC 5 cut(s) 225, 258, 399, 526, 817
BstMWI GCNNNNNNNGC 3 cut(s) 239, 518, 714
BstNI CCWGG 2 cut(s) 134, 857
BstNSI RCATGY 1 cut(s) 24
BstPI GGTNACC 1 cut(s) 554
BstSCI CCNGG 4 cut(s) 132, 709, 763, 855
BstSFI CTRYAG 1 cut(s) 749
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 399, 817
BstYI RGATCY 2 cut(s) 399, 817
BsuRI GGCC 4 cut(s) 145, 352, 708, 755
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 103, 753
Cfr13I GGNCC 5 cut(s) 350, 472, 707, 761, 859
CseI GACGC 1 cut(s) 518
Csp6I GTAC 1 cut(s) 538
CviAII CATG 5 cut(s) 21, 404, 458, 532, 643
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 3 cut(s) 46, 870, 895
DpnI GATC 5 cut(s) 227, 260, 401, 528, 819
DpnII GATC 5 cut(s) 225, 258, 399, 526, 817
DriI GACNNNNNGTC 1 cut(s) 767
EaeI YGGCCR 1 cut(s) 143
Eam1105I GACNNNNNGTC 1 cut(s) 767
Eco130I CCWWGG 3 cut(s) 176, 802, 834
Eco47I GGWCC 3 cut(s) 472, 761, 859
Eco57I CTGAAG 1 cut(s) 362
Eco91I GGTNACC 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 707
EcoO65I GGTNACC 1 cut(s) 554
EcoRII CCWGG 2 cut(s) 132, 855
EcoT14I CCWWGG 3 cut(s) 176, 802, 834
ErhI CCWWGG 3 cut(s) 176, 802, 834
FaeI CATG 5 cut(s) 24, 407, 461, 535, 646
FaqI GGGAC 1 cut(s) 521
FatI CATG 5 cut(s) 20, 403, 457, 531, 642
FauNDI CATATG 2 cut(s) 122, 571
FbaI TGATCA 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 715
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 1 cut(s) 715
FspBI CTAG 2 cut(s) 102, 773
GluI GCNGC 1 cut(s) 715
HaeIII GGCC 4 cut(s) 145, 352, 708, 755
HapII CCGG 3 cut(s) 711, 759, 765
HgaI GACGC 1 cut(s) 518
Hin1II CATG 5 cut(s) 24, 407, 461, 535, 646
HinfI GANTC 2 cut(s) 566, 784
HpaII CCGG 3 cut(s) 711, 759, 765
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 2 cut(s) 540, 689
Hpy188I TCNGA 2 cut(s) 263, 873
Hpy188III TCNNGA 5 cut(s) 9, 203, 334, 524, 842
Hpy8I GTNNAC 2 cut(s) 540, 689
HpyAV CCTTC 1 cut(s) 464
HpyCH4V TGCA 8 cut(s) 20, 27, 128, 233, 280, 324, 611, 751
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 518, 714
HpyF3I CTNAG 3 cut(s) 46, 870, 895
Hsp92II CATG 5 cut(s) 24, 407, 461, 535, 646
Ksp22I TGATCA 1 cut(s) 225
Kzo9I GATC 5 cut(s) 225, 258, 399, 526, 817
MaeI CTAG 2 cut(s) 102, 773
MaeIII GTNAC 3 cut(s) 554, 664, 781
MalI GATC 5 cut(s) 227, 260, 401, 528, 819
MboI GATC 5 cut(s) 225, 258, 399, 526, 817
MboII GAAGA 4 cut(s) 223, 368, 403, 475
MflI RGATCY 2 cut(s) 399, 817
MlsI TGGCCA 1 cut(s) 145
MluCI AATT 5 cut(s) 115, 192, 264, 288, 298
MluNI TGGCCA 1 cut(s) 145
MlyI GAGTC 2 cut(s) 575, 778
MmeI TCCRAC 1 cut(s) 845
MnlI CCTC 2 cut(s) 77, 263
Mox20I TGGCCA 1 cut(s) 145
MscI TGGCCA 1 cut(s) 145
MseI TTAA 2 cut(s) 198, 900
Msp20I TGGCCA 1 cut(s) 145
MspI CCGG 3 cut(s) 711, 759, 765
MspR9I CCNGG 4 cut(s) 134, 711, 765, 857
Mva1269I GAATGC 3 cut(s) 27, 242, 454
MvaI CCWGG 2 cut(s) 134, 857
MwoI GCNNNNNNNGC 3 cut(s) 239, 518, 714
NciI CCSGG 2 cut(s) 711, 765
NdeI CATATG 2 cut(s) 122, 571
NdeII GATC 5 cut(s) 225, 258, 399, 526, 817
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 5 cut(s) 24, 407, 461, 535, 646
NlaIV GGNNCC 4 cut(s) 474, 709, 763, 819
NmuCI GTSAC 2 cut(s) 664, 781
NspI RCATGY 1 cut(s) 24
PctI GAATGC 3 cut(s) 27, 242, 454
PkrI GCNGC 1 cut(s) 716
PleI GAGTC 2 cut(s) 574, 778
PpsI GAGTC 2 cut(s) 574, 778
PsiI TTATAA 2 cut(s) 297, 830
Psp6I CCWGG 2 cut(s) 132, 855
PspEI GGTNACC 1 cut(s) 554
PspGI CCWGG 2 cut(s) 132, 855
PspN4I GGNNCC 4 cut(s) 474, 709, 763, 819
PspPI GGNCC 5 cut(s) 350, 472, 707, 761, 859
PstI CTGCAG 1 cut(s) 753
PsuI RGATCY 2 cut(s) 399, 817
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
SaqAI TTAA 2 cut(s) 198, 900
SatI GCNGC 1 cut(s) 715
Sau3AI GATC 5 cut(s) 225, 258, 399, 526, 817
Sau96I GGNCC 5 cut(s) 350, 472, 707, 761, 859
SbfI CCTGCAGG 1 cut(s) 753
SchI GAGTC 2 cut(s) 575, 778
ScrFI CCNGG 4 cut(s) 134, 711, 765, 857
SdaI CCTGCAGG 1 cut(s) 753
SfcI CTRYAG 1 cut(s) 749
SinI GGWCC 3 cut(s) 472, 761, 859
Sse8387I CCTGCAGG 1 cut(s) 753
Sse9I AATT 5 cut(s) 115, 192, 264, 288, 298
SsiI CCGC 3 cut(s) 369, 470, 714
SspMI CTAG 2 cut(s) 102, 773
StyD4I CCNGG 4 cut(s) 132, 709, 763, 855
StyI CCWWGG 3 cut(s) 176, 802, 834
TaqI TCGA 4 cut(s) 8, 69, 82, 95
TasI AATT 5 cut(s) 115, 192, 264, 288, 298
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 717
Tru1I TTAA 2 cut(s) 198, 900
Tru9I TTAA 2 cut(s) 198, 900
TseFI GTSAC 2 cut(s) 664, 781
Tsp45I GTSAC 2 cut(s) 664, 781
TspDTI ATGAA 5 cut(s) 109, 264, 446, 659, 855
TspGWI ACGGA 1 cut(s) 353
VpaK11BI GGWCC 3 cut(s) 472, 761, 859
XapI RAATTY 1 cut(s) 192
XceI RCATGY 1 cut(s) 24
XspI CTAG 2 cut(s) 102, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.