FvH4_4g30431

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004486
Physical Location & Seq
Forward (+)
30060050 .. 30062546
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30431.t1

Sequence Viewer

Length: 1578 bp
ATGGGGTCTAGTATAGCATTTGCATGTTTCATTTTTTTGTCATTGTTCTCAGTAGCTTCAGCTGAATATGTCGATGTCAGGATTCGAGGTGTGGTTTCGATAGCTAACACAGATGACAATTTCATATGTGCAACCCTGGATTGGTGGCCGACTGATAAATGTGATTATGGTCAATGTCCTTGGGGAAAGGCTGGAATTTTCAATCTGGATTTGGAGAACAAGACCTTGATCAATGCAGTTAAGGCATTCGGTTCAATAAGGCTCAGAATTGGAGGTTCATTGCAAGATAGTGTTACTTATGGATTTGGATATGGAGTGAAAGAATGCAACCGATGGAAGAAAGATAGTTCAGGGCTTTTCGGGTTCAGTGGCGCTTGCCTTGACCCGAATAGATGGGATGAGATCCATGACTTCTTCAACAAAACACAAGCCAAACTCTGGTTTGGATTGAATGCCCTTCAAGGGAAGAAACCGGACTCGAAAGACAAGGTTCTTTGGGTTGGTGATTGGGATGCAAGAAACGCTCGTGACCTCATGGGGTACACCATCTCAAAGGGTTACCCTGTTGAGTCATATGAGCTAGGAAATGAGCTATGCGGGGGTGGCGTTGCTGCAAGAATAGAAGCTCAACAATATGCTAAAGACATGAAAAAGCTCAAACAACTTGTGACAGAGATGTTCCCAGATGGTAAACAGCCAAAGGTTTTAGGCCCCGGCGGCTTTTATGATAAACAGTGGTTCAATGACTTCCTGCTGGCCACCGGACCTGGTGTCCTAGACGGCGTGACTCATCACACTTACAATCTTGGTCCTGGTGTTGATCCAACCCTCGTTAAGAAGGTTCAAGACCCATATTACCTGGACAATTCAGCTCAGACATATAAGGATGCTGCAGACTCAGTTAAACAGTTTGCACCACGGACAGACCCTTGGGTTGGTGAGGCTGGTGGAGCTTACAACAGTGGTGGCAAAGATGTTTCACATACCTTTGCTAATGGCTTTTGGTATTTGGACCAACTTGGTATGACAGCAAGCTACAGTCACAAGGTTTTCTGCAGACAAGCCTTGATTGGAGGAAACTATGCCCTACTTAATACCACATCTTTCATCCCTAATCCTGACTACTATGGTGCACTCCTATGGCATAGACTGATGGGAAATAGCGTGCTCTCTACCGTTCATTATGGCTCTCCTTTCTTGCGTGCCTACTCTCATTGTTCAAAGAAAAAGCCTGGAATCACTCTGCTTTTGATCAACATGTCCAACTCTACTACCTTCGATGTTGATGTTCTCCCCGAGTTCAATTTGCATCCTTGGCAAAGATATGAAGAACAATCTGACACAAAATCAACTGGTGCATCAATGAGAGAAGAGTATCATTTGACACCAGAAGGTGGCAATATCCAAAGTGATGTGCTGCTACTCAATGGGACTCCATTGAAGCTCACAGAATCATTCGACATTCCTGAAATGCAACCAAAACTAGTGGATCCTACCTCTGCAGTCAGTGTTGCACCAGATTCGTTCGTCTTTGTATCTATCAAGGACTTCCATGCCCCTGCATGCGCGCTTAGTTAG

Protein Analysis

526

Amino Acids

58.09

Weight (kDa)

5.8

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 25 - 341 5.3e-139 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 438, 1394
AccII CGCG 1 cut(s) 1568
AciI CCGC 2 cut(s) 597, 717
AclWI GGATC 4 cut(s) 397, 815, 1484, 1497
AcoI YGGCCR 2 cut(s) 146, 756
AcsI RAATTY 1 cut(s) 195
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 5 cut(s) 141, 438, 462, 935, 1394
AflIII ACRYGT 1 cut(s) 1257
AhdI GACNNNNNGTC 1 cut(s) 770
AhlI ACTAGT 1 cut(s) 1483
AjnI CCWGG 5 cut(s) 135, 766, 811, 858, 1231
Alw21I GWGCWC 2 cut(s) 1135, 1170
Alw44I GTGCAC 1 cut(s) 1131
AlwI GGATC 4 cut(s) 397, 815, 1484, 1497
Ama87I CYCGRG 1 cut(s) 1295
AoxI GGCC 3 cut(s) 146, 709, 756
ApaLI GTGCAC 1 cut(s) 1131
ApeKI GCWGC 3 cut(s) 611, 890, 1417
ApoI RAATTY 1 cut(s) 195
AspLEI GCGC 3 cut(s) 374, 1568, 1570
AspS9I GGNCC 4 cut(s) 710, 764, 809, 1012
AsuC2I CCSGG 1 cut(s) 714
AsuHPI GGTGA 2 cut(s) 515, 950
AvaI CYCGRG 1 cut(s) 1295
AvaII GGWCC 3 cut(s) 764, 809, 1012
BaeGI GKGCMC 1 cut(s) 1135
BalI TGGCCA 1 cut(s) 758
BamHI GGATCC 1 cut(s) 1489
BauI CACGAG 1 cut(s) 525
Bbv12I GWGCWC 2 cut(s) 1135, 1170
BbvI GCAGC 3 cut(s) 598, 877, 1404
BccI CCATC 5 cut(s) 327, 387, 554, 680, 1147
BceAI ACGGC 1 cut(s) 796
BcgI CGANNNNNNTGC 2 cut(s) 1503, 1537
BciT130I CCWGG 5 cut(s) 137, 768, 813, 860, 1233
BclI TGATCA 2 cut(s) 228, 1251
BcnI CCSGG 1 cut(s) 714
BcuI ACTAGT 1 cut(s) 1483
BfaI CTAG 4 cut(s) 9, 581, 776, 1484
BfmI CTRYAG 4 cut(s) 891, 1036, 1054, 1500
BfoI RGCGCY 1 cut(s) 375
BglI GCCNNNNNGGC 1 cut(s) 717
BisI GCNGC 4 cut(s) 612, 718, 891, 1418
BlsI GCNGC 4 cut(s) 613, 719, 892, 1419
Bme1390I CCNGG 6 cut(s) 137, 714, 768, 813, 860, 1233
Bme18I GGWCC 3 cut(s) 764, 809, 1012
BmeRI GACNNNNNGTC 1 cut(s) 770
BmeT110I CYCGRG 1 cut(s) 1295
BmgT120I GGNCC 4 cut(s) 710, 764, 809, 1012
BmiI GGNNCC 2 cut(s) 712, 1491
BmrFI CCNGG 6 cut(s) 137, 714, 768, 813, 860, 1233
BmsI GCATC 4 cut(s) 502, 877, 1318, 1367
BpuMI CCSGG 1 cut(s) 714
BsaJI CCNNGG 6 cut(s) 135, 179, 712, 917, 929, 1313
BsaWI WCCGGW 2 cut(s) 472, 761
Bsc4I CCNNNNNNNGG 5 cut(s) 141, 438, 462, 935, 1394
Bse1I ACTGG 1 cut(s) 1357
Bse3DI GCAATG 1 cut(s) 278
BseBI CCWGG 5 cut(s) 137, 768, 813, 860, 1233
BseDI CCNNGG 6 cut(s) 135, 179, 712, 917, 929, 1313
BseGI GGATG 5 cut(s) 403, 517, 892, 1107, 1309
BseLI CCNNNNNNNGG 5 cut(s) 141, 438, 462, 935, 1394
BseMI GCAATG 1 cut(s) 278
BseMII CTCAG 4 cut(s) 63, 277, 887, 912
BseNI ACTGG 1 cut(s) 1357
BsePI GCGCGC 1 cut(s) 1566
BseSI GKGCMC 1 cut(s) 1135
BseXI GCAGC 3 cut(s) 598, 877, 1404
Bsh1236I CGCG 1 cut(s) 1568
BshFI GGCC 3 cut(s) 148, 711, 758
BsiHKAI GWGCWC 2 cut(s) 1135, 1170
BsiHKCI CYCGRG 1 cut(s) 1295
BsiSI CCGG 3 cut(s) 473, 714, 762
BslFI GGGAC 1 cut(s) 1444
BslI CCNNNNNNNGG 5 cut(s) 141, 438, 462, 935, 1394
BsmFI GGGAC 1 cut(s) 1444
BsmI GAATGC 3 cut(s) 245, 329, 457
BsnI GGCC 3 cut(s) 148, 711, 758
BsoBI CYCGRG 1 cut(s) 1295
Bsp1286I GDGCHC 2 cut(s) 1135, 1170
Bsp143I GATC 5 cut(s) 228, 402, 820, 1251, 1489
BspACI CCGC 2 cut(s) 597, 717
BspANI GGCC 3 cut(s) 148, 711, 758
BspCNI CTCAG 4 cut(s) 62, 276, 886, 911
BspFNI CGCG 1 cut(s) 1568
BspLI GGNNCC 2 cut(s) 712, 1491
BspMAI CTGCAG 3 cut(s) 895, 1058, 1504
BspPI GGATC 4 cut(s) 397, 815, 1484, 1497
BsrDI GCAATG 1 cut(s) 278
BsrI ACTGG 1 cut(s) 1357
BssECI CCNNGG 6 cut(s) 135, 179, 712, 917, 929, 1313
BssHII GCGCGC 1 cut(s) 1566
BssMI GATC 5 cut(s) 228, 402, 820, 1251, 1489
BssSI CACGAG 1 cut(s) 525
BssT1I CCWWGG 3 cut(s) 179, 929, 1313
Bst2BI CACGAG 1 cut(s) 525
Bst2UI CCWGG 5 cut(s) 137, 768, 813, 860, 1233
Bst4CI ACNGT 5 cut(s) 735, 909, 962, 1040, 1177
Bst6I CTCTTC 1 cut(s) 1365
BstC8I GCNNGC 7 cut(s) 376, 756, 1033, 1166, 1203, 1564, 1568
BstDEI CTNAG 5 cut(s) 49, 263, 873, 898, 1571
BstDSI CCRYGG 1 cut(s) 917
BstEII GGTNACC 1 cut(s) 557
BstF5I GGATG 5 cut(s) 403, 517, 892, 1107, 1309
BstFNI CGCG 1 cut(s) 1568
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 3 cut(s) 374, 1568, 1570
BstKTI GATC 5 cut(s) 231, 405, 823, 1254, 1492
BstMBI GATC 5 cut(s) 228, 402, 820, 1251, 1489
BstMWI GCNNNNNNNGC 6 cut(s) 242, 521, 603, 717, 950, 1315
BstNI CCWGG 5 cut(s) 137, 768, 813, 860, 1233
BstNSI RCATGY 3 cut(s) 27, 1261, 1566
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 6 cut(s) 135, 712, 766, 811, 858, 1231
BstSFI CTRYAG 4 cut(s) 891, 1036, 1054, 1500
BstSLI GKGCMC 1 cut(s) 1135
BstUI CGCG 1 cut(s) 1568
BstV1I GCAGC 3 cut(s) 598, 877, 1404
BstX2I RGATCY 2 cut(s) 402, 1489
BstYI RGATCY 2 cut(s) 402, 1489
BsuRI GGCC 3 cut(s) 148, 711, 758
BtgI CCRYGG 1 cut(s) 917
BtsCI GGATG 5 cut(s) 403, 517, 892, 1107, 1309
BtsIMutI CAGTG 4 cut(s) 373, 740, 967, 1513
Cac8I GCNNGC 7 cut(s) 376, 756, 1033, 1166, 1203, 1564, 1568
CfoI GCGC 3 cut(s) 374, 1568, 1570
Cfr13I GGNCC 4 cut(s) 710, 764, 809, 1012
CsiI ACCWGGT 1 cut(s) 766
Csp6I GTAC 1 cut(s) 541
CspCI CAANNNNNGTGG 4 cut(s) 946, 981, 1467, 1502
CviAII CATG 7 cut(s) 24, 407, 535, 646, 1258, 1553, 1563
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 5 cut(s) 49, 263, 873, 898, 1571
DpnI GATC 5 cut(s) 230, 404, 822, 1253, 1491
DpnII GATC 5 cut(s) 228, 402, 820, 1251, 1489
DriI GACNNNNNGTC 1 cut(s) 770
EaeI YGGCCR 2 cut(s) 146, 756
Eam1104I CTCTTC 1 cut(s) 1365
Eam1105I GACNNNNNGTC 1 cut(s) 770
EarI CTCTTC 1 cut(s) 1365
Eco130I CCWWGG 3 cut(s) 179, 929, 1313
Eco47I GGWCC 3 cut(s) 764, 809, 1012
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 1295
Eco91I GGTNACC 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 710
EcoO65I GGTNACC 1 cut(s) 557
EcoRII CCWGG 5 cut(s) 135, 766, 811, 858, 1231
EcoT14I CCWWGG 3 cut(s) 179, 929, 1313
ErhI CCWWGG 3 cut(s) 179, 929, 1313
FaeI CATG 7 cut(s) 27, 410, 538, 649, 1261, 1556, 1566
FaqI GGGAC 1 cut(s) 1444
FatI CATG 7 cut(s) 23, 406, 534, 645, 1257, 1552, 1562
FauI CCCGC 1 cut(s) 590
FauNDI CATATG 2 cut(s) 125, 574
FbaI TGATCA 2 cut(s) 228, 1251
Fnu4HI GCNGC 4 cut(s) 612, 718, 891, 1418
FokI GGATG 5 cut(s) 410, 524, 899, 1094, 1296
Fsp4HI GCNGC 4 cut(s) 612, 718, 891, 1418
FspBI CTAG 4 cut(s) 9, 581, 776, 1484
GlaI GCGC 3 cut(s) 373, 1567, 1569
GluI GCNGC 4 cut(s) 612, 718, 891, 1418
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 3 cut(s) 148, 711, 758
HapII CCGG 3 cut(s) 473, 714, 762
HhaI GCGC 3 cut(s) 374, 1568, 1570
Hin1II CATG 7 cut(s) 27, 410, 538, 649, 1261, 1556, 1566
Hin6I GCGC 3 cut(s) 372, 1566, 1568
HinP1I GCGC 3 cut(s) 372, 1566, 1568
HinfI GANTC 9 cut(s) 82, 476, 569, 787, 896, 1236, 1432, 1451, 1520
HpaII CCGG 3 cut(s) 473, 714, 762
HphI GGTGA 2 cut(s) 515, 950
Hpy166II GTNNAC 3 cut(s) 543, 692, 1133
Hpy188I TCNGA 3 cut(s) 266, 876, 1339
Hpy188III TCNNGA 6 cut(s) 79, 206, 527, 845, 1118, 1466
Hpy8I GTNNAC 3 cut(s) 543, 692, 1133
HpyAV CCTTC 4 cut(s) 467, 832, 1285, 1385
HpyCH4III ACNGT 5 cut(s) 735, 909, 962, 1040, 1177
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 521, 603, 717, 950, 1315
HpyF3I CTNAG 5 cut(s) 49, 263, 873, 898, 1571
Hsp92II CATG 7 cut(s) 27, 410, 538, 649, 1261, 1556, 1566
HspAI GCGC 3 cut(s) 372, 1566, 1568
Ksp22I TGATCA 2 cut(s) 228, 1251
Kzo9I GATC 5 cut(s) 228, 402, 820, 1251, 1489
LmnI GCTCC 1 cut(s) 950
Lsp1109I GCAGC 3 cut(s) 598, 877, 1404
LweI GCATC 4 cut(s) 502, 877, 1318, 1367
MabI ACCWGGT 1 cut(s) 766
MaeI CTAG 4 cut(s) 9, 581, 776, 1484
MaeIII GTNAC 6 cut(s) 292, 527, 557, 667, 784, 1040
MalI GATC 5 cut(s) 230, 404, 822, 1253, 1491
MboI GATC 5 cut(s) 228, 402, 820, 1251, 1489
MboII GAAGA 5 cut(s) 349, 406, 478, 1340, 1382
MflI RGATCY 2 cut(s) 402, 1489
MhlI GDGCHC 2 cut(s) 1135, 1170
MlsI TGGCCA 1 cut(s) 758
MluCI AATT 5 cut(s) 118, 195, 267, 865, 1303
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 5 cut(s) 470, 578, 781, 890, 1426
MmeI TCCRAC 2 cut(s) 848, 1287
MnlI CCTC 7 cut(s) 80, 266, 542, 839, 934, 1067, 1507
Mox20I TGGCCA 1 cut(s) 758
MscI TGGCCA 1 cut(s) 758
MseI TTAA 4 cut(s) 240, 834, 903, 1092
MslI CAYNNNNRTG 2 cut(s) 22, 1138
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 3 cut(s) 473, 714, 762
MspR9I CCNGG 6 cut(s) 137, 714, 768, 813, 860, 1233
Mva1269I GAATGC 3 cut(s) 245, 329, 457
MvaI CCWGG 5 cut(s) 137, 768, 813, 860, 1233
MvnI CGCG 1 cut(s) 1568
MwoI GCNNNNNNNGC 6 cut(s) 242, 521, 603, 717, 950, 1315
NciI CCSGG 1 cut(s) 714
NdeI CATATG 2 cut(s) 125, 574
NdeII GATC 5 cut(s) 228, 402, 820, 1251, 1489
NlaIII CATG 7 cut(s) 27, 410, 538, 649, 1261, 1556, 1566
NlaIV GGNNCC 2 cut(s) 712, 1491
NmuCI GTSAC 4 cut(s) 527, 667, 784, 1040
NspI RCATGY 3 cut(s) 27, 1261, 1566
PaeI GCATGC 1 cut(s) 1566
PauI GCGCGC 1 cut(s) 1566
PciI ACATGT 1 cut(s) 1257
PctI GAATGC 3 cut(s) 245, 329, 457
PfeI GAWTC 4 cut(s) 82, 1236, 1451, 1520
PflMI CCANNNNNTGG 2 cut(s) 438, 1394
PkrI GCNGC 4 cut(s) 613, 719, 892, 1419
PleI GAGTC 5 cut(s) 470, 577, 781, 890, 1426
PpsI GAGTC 5 cut(s) 470, 577, 781, 890, 1426
PscI ACATGT 1 cut(s) 1257
Psp6I CCWGG 5 cut(s) 135, 766, 811, 858, 1231
PspEI GGTNACC 1 cut(s) 557
PspGI CCWGG 5 cut(s) 135, 766, 811, 858, 1231
PspN4I GGNNCC 2 cut(s) 712, 1491
PspPI GGNCC 4 cut(s) 710, 764, 809, 1012
PstI CTGCAG 3 cut(s) 895, 1058, 1504
PsuI RGATCY 2 cut(s) 402, 1489
PteI GCGCGC 1 cut(s) 1566
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
RseI CAYNNNNRTG 2 cut(s) 22, 1138
SaqAI TTAA 4 cut(s) 240, 834, 903, 1092
SatI GCNGC 4 cut(s) 612, 718, 891, 1418
Sau3AI GATC 5 cut(s) 228, 402, 820, 1251, 1489
Sau96I GGNCC 4 cut(s) 710, 764, 809, 1012
SchI GAGTC 5 cut(s) 470, 578, 781, 890, 1426
ScrFI CCNGG 6 cut(s) 137, 714, 768, 813, 860, 1233
SduI GDGCHC 2 cut(s) 1135, 1170
SexAI ACCWGGT 1 cut(s) 766
SfaNI GCATC 4 cut(s) 502, 877, 1318, 1367
SfcI CTRYAG 4 cut(s) 891, 1036, 1054, 1500
SinI GGWCC 3 cut(s) 764, 809, 1012
SmiMI CAYNNNNRTG 2 cut(s) 22, 1138
SpeI ACTAGT 1 cut(s) 1483
SphI GCATGC 1 cut(s) 1566
Sse9I AATT 5 cut(s) 118, 195, 267, 865, 1303
SsiI CCGC 2 cut(s) 597, 717
SspMI CTAG 4 cut(s) 9, 581, 776, 1484
StyD4I CCNGG 6 cut(s) 135, 712, 766, 811, 858, 1231
StyI CCWWGG 3 cut(s) 179, 929, 1313
TaaI ACNGT 5 cut(s) 735, 909, 962, 1040, 1177
TaqI TCGA 6 cut(s) 72, 85, 98, 479, 1278, 1458
TasI AATT 5 cut(s) 118, 195, 267, 865, 1303
TauI GCSGC 1 cut(s) 720
TfiI GAWTC 4 cut(s) 82, 1236, 1451, 1520
Tru1I TTAA 4 cut(s) 240, 834, 903, 1092
Tru9I TTAA 4 cut(s) 240, 834, 903, 1092
TscAI CASTG 4 cut(s) 373, 740, 967, 1513
TseFI GTSAC 4 cut(s) 527, 667, 784, 1040
TseI GCWGC 3 cut(s) 611, 890, 1417
Tsp45I GTSAC 4 cut(s) 527, 667, 784, 1040
TspDTI ATGAA 7 cut(s) 19, 112, 267, 662, 1096, 1169, 1341
TspGWI ACGGA 1 cut(s) 934
TspRI CASTG 4 cut(s) 373, 740, 967, 1513
Van91I CCANNNNNTGG 2 cut(s) 438, 1394
VneI GTGCAC 1 cut(s) 1131
VpaK11BI GGWCC 3 cut(s) 764, 809, 1012
XapI RAATTY 1 cut(s) 195
XceI RCATGY 3 cut(s) 27, 1261, 1566
XspI CTAG 4 cut(s) 9, 581, 776, 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.