RLG00000006333

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004486
Physical Location & Seq
Reverse (-)
6313193 .. 6315462
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006333

Sequence Viewer

Length: 1569 bp
ATGGGGTCGAGAGCTATTGCATGTTGCATTCTGTTGTCATTGTTCTCAGTAGCTTTAGCTGAATATGTCGATGTCAGGGTTCGAGGAGTGGTTTCGATAGCTAGCACAGATGACAATTTCATATGCGCAACCCTGGATTGGTGGCCAACTAATAAATGTGATTACAATCAATGTCCTTGGGGAAAAGCTGGAATTTTCAATCTGGATTTGAAGAACAAGACCTTGATCAATGCAGTAAAGGCATTCAATACAGTAAGGATCAGAATTGGAGGTTCATTGCAAGACCAAATTATTTATAATTTTGGTTATGGAGTGAAAGACTGCAACCAAATCGTGAAAGATGGTTCTGGGCCTTTCTTGTTCAGTGGCGGTTGTCTTCAGCCGAAAAGATGGGATGAGATCCATGAGTTCTTCAACGAAACACAAGCCAAACTCTGGTTTGGATTGAATGCCCTTCATGGGAAGAAAACGGACCCGAAAGACAAGATTCTTTGGGTTGGTGATTGGGACGCAAAAAACGCTCGTGATCTCATGGAGTACACCATCTCAAAGGGTTACCCTGTTGAGTCATATGAGCTTGGAAACGAGTTATGTGGGAGTGGGGTAGGTGCAAGAATAGAAGCTGAACAATATGCCAAAGACATGAAAAAGCTCAAACAACTTGTGACAGAGTTGTTCCCAGATGGTAAACAGCCAAAGGTATTAGGCCCCGGCGGCTTTTATGAGAAAAAGTGGTTCAATGACTTCCTGCAGGCCACCGGACCCGGTGTCCTAGACGGTGTGACTCATCACACTTACGACCTTGGTGCTGGTGTTGATCCAACCCTTATAACCAAGGTTCAAGACCCATTTTACCTGGACCATTCAGCTCAGACATATAATGATGTGGCAAACTCAGTTAAACAGTTTGCCCCATGGACAGACCCTTGGGTTGGTGAGGCTGGTGGAGCTTACAACAGTGGTGGCAAAGATGTTTCACATACCTTTGCTAATGGCTTTTGGTATTTGGACCAACTTGGTATGACAGCAAGCTACAGTCACAAGGTTTACTGCAGACAAGCCTTGATTGGAGGGAACTACGCCCTACTTAATACCACATCTTTCATCCCTAATCCTGACTACTATGGTGCACTCCTATGGCATAGACTGATGGGAAATAAAGTGCTCTCTACCGTCCATTATGGCTCTCCCTTCTTGCGTGCATATACTCATTGTTCAAAGAAAAAGCCTGGGGTTACTCTGCTTTTGATCAACATGTCCAACTCTACTACCTTCGATGTTGAAGTTTTCCCTGAGTTCAATCTGCATCCTTGGCAAAGATATGAGGTACAATCGGACACAAAATCAGCTGGTGCACCAATGAGAGAAGAGTATCATTTGACACCAGAAGGTGGCAATATCCAAAGTGATGTGCTGCTACTGAATGGGACTCCATTGAAGCTCACAGAGTCATCCGACATTCCTGAACTGCAACCGAAACTAGTGGATCCTACCTCTGCAGTCAGTGTGGCACCACAGTCGTTCGTCTTTGTATCTATCAAGGACTTCCATGCCCCTGCATGCGCTTAG

Protein Analysis

523

Amino Acids

57.96

Weight (kDa)

6.02

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 24 - 340 1.2e-141 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 297, 830
Acc16I TGCGCA 1 cut(s) 127
AccB1I GGYRCC 1 cut(s) 1510
AccB7I CCANNNNNTGG 2 cut(s) 435, 1391
AciI CCGC 2 cut(s) 369, 714
AclWI GGATC 5 cut(s) 266, 394, 812, 1481, 1494
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 2 cut(s) 539, 1329
AfiI CCNNNNNNNGG 5 cut(s) 138, 435, 459, 932, 1391
AflIII ACRYGT 1 cut(s) 1254
AhdI GACNNNNNGTC 1 cut(s) 767
AhlI ACTAGT 1 cut(s) 1480
AjnI CCWGG 3 cut(s) 132, 855, 1228
Alw21I GWGCWC 3 cut(s) 1132, 1167, 1357
Alw44I GTGCAC 2 cut(s) 1128, 1353
AlwI GGATC 5 cut(s) 266, 394, 812, 1481, 1494
AoxI GGCC 4 cut(s) 143, 350, 706, 753
ApaLI GTGCAC 2 cut(s) 1128, 1353
ApeKI GCWGC 1 cut(s) 1414
ApoI RAATTY 1 cut(s) 192
AspLEI GCGC 2 cut(s) 128, 1565
AspS9I GGNCC 6 cut(s) 350, 472, 707, 761, 859, 1009
AsuC2I CCSGG 2 cut(s) 711, 765
AsuHPI GGTGA 2 cut(s) 512, 947
AsuNHI GCTAGC 1 cut(s) 101
AvaII GGWCC 4 cut(s) 472, 761, 859, 1009
BaeGI GKGCMC 2 cut(s) 1132, 1357
BalI TGGCCA 1 cut(s) 145
BamHI GGATCC 1 cut(s) 1486
BanI GGYRCC 1 cut(s) 1510
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 368
Bbv12I GWGCWC 3 cut(s) 1132, 1167, 1357
BbvI GCAGC 1 cut(s) 1401
BccI CCATC 5 cut(s) 335, 384, 551, 677, 1144
BcgI CGANNNNNNTGC 6 cut(s) 313, 347, 788, 822, 1500, 1534
BciT130I CCWGG 3 cut(s) 134, 857, 1230
BclI TGATCA 2 cut(s) 225, 1248
BcnI CCSGG 2 cut(s) 711, 765
BcuI ACTAGT 1 cut(s) 1480
BfaI CTAG 3 cut(s) 102, 773, 1481
BfmI CTRYAG 4 cut(s) 749, 1033, 1051, 1497
BglI GCCNNNNNGGC 1 cut(s) 714
BisI GCNGC 2 cut(s) 715, 1415
BlsI GCNGC 2 cut(s) 716, 1416
Bme1390I CCNGG 5 cut(s) 134, 711, 765, 857, 1230
Bme18I GGWCC 4 cut(s) 472, 761, 859, 1009
BmeRI GACNNNNNGTC 1 cut(s) 767
BmgT120I GGNCC 6 cut(s) 350, 472, 707, 761, 859, 1009
BmiI GGNNCC 5 cut(s) 474, 709, 763, 1488, 1512
BmrFI CCNGG 5 cut(s) 134, 711, 765, 857, 1230
BmsI GCATC 1 cut(s) 1315
BmtI GCTAGC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 368
BpuMI CCSGG 2 cut(s) 711, 765
BsaBI GATNNNNATC 1 cut(s) 165
BsaJI CCNNGG 9 cut(s) 132, 176, 709, 802, 834, 914, 926, 1229, 1310
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 435, 459, 932, 1391
Bse3DI GCAATG 1 cut(s) 275
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 3 cut(s) 134, 857, 1230
BseDI CCNNGG 9 cut(s) 132, 176, 709, 802, 834, 914, 926, 1229, 1310
BseGI GGATG 4 cut(s) 400, 1104, 1306, 1451
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 5 cut(s) 138, 435, 459, 932, 1391
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 4 cut(s) 60, 884, 909, 1284
BseRI GAGGAG 1 cut(s) 99
BseSI GKGCMC 2 cut(s) 1132, 1357
BseXI GCAGC 1 cut(s) 1401
BshFI GGCC 4 cut(s) 145, 352, 708, 755
BshNI GGYRCC 1 cut(s) 1510
BsiHKAI GWGCWC 3 cut(s) 1132, 1167, 1357
BsiSI CCGG 3 cut(s) 711, 759, 765
BslFI GGGAC 2 cut(s) 521, 1441
BslI CCNNNNNNNGG 5 cut(s) 138, 435, 459, 932, 1391
BsmFI GGGAC 2 cut(s) 521, 1441
BsmI GAATGC 3 cut(s) 27, 242, 454
BsnI GGCC 4 cut(s) 145, 352, 708, 755
Bsp1286I GDGCHC 3 cut(s) 1132, 1167, 1357
Bsp143I GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1486
Bsp19I CCATGG 1 cut(s) 914
BspACI CCGC 2 cut(s) 369, 714
BspANI GGCC 4 cut(s) 145, 352, 708, 755
BspCNI CTCAG 4 cut(s) 59, 883, 908, 1285
BspLI GGNNCC 5 cut(s) 474, 709, 763, 1488, 1512
BspMAI CTGCAG 3 cut(s) 753, 1055, 1501
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 5 cut(s) 266, 394, 812, 1481, 1494
BspT107I GGYRCC 1 cut(s) 1510
BsrDI GCAATG 1 cut(s) 275
BssECI CCNNGG 9 cut(s) 132, 176, 709, 802, 834, 914, 926, 1229, 1310
BssMI GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1486
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 134, 857, 1230
Bst4CI ACNGT 7 cut(s) 253, 779, 906, 959, 1037, 1174, 1518
Bst6I CTCTTC 1 cut(s) 1362
BstC8I GCNNGC 5 cut(s) 103, 753, 1030, 1200, 1561
BstDEI CTNAG 5 cut(s) 46, 870, 895, 1293, 1566
BstDSI CCRYGG 1 cut(s) 914
BstEII GGTNACC 1 cut(s) 554
BstF5I GGATG 4 cut(s) 400, 1104, 1306, 1451
BstHHI GCGC 2 cut(s) 128, 1565
BstKTI GATC 7 cut(s) 228, 261, 402, 529, 820, 1251, 1489
BstMBI GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1486
BstMWI GCNNNNNNNGC 5 cut(s) 239, 518, 714, 947, 1312
BstNI CCWGG 3 cut(s) 134, 857, 1230
BstNSI RCATGY 3 cut(s) 24, 1258, 1563
BstPI GGTNACC 1 cut(s) 554
BstSCI CCNGG 5 cut(s) 132, 709, 763, 855, 1228
BstSFI CTRYAG 4 cut(s) 749, 1033, 1051, 1497
BstSLI GKGCMC 2 cut(s) 1132, 1357
BstV1I GCAGC 1 cut(s) 1401
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 399, 1486
BstYI RGATCY 2 cut(s) 399, 1486
BsuRI GGCC 4 cut(s) 145, 352, 708, 755
BtgI CCRYGG 1 cut(s) 914
BtsCI GGATG 4 cut(s) 400, 1104, 1306, 1451
BtsIMutI CAGTG 3 cut(s) 370, 964, 1510
Cac8I GCNNGC 5 cut(s) 103, 753, 1030, 1200, 1561
CfoI GCGC 2 cut(s) 128, 1565
Cfr13I GGNCC 6 cut(s) 350, 472, 707, 761, 859, 1009
CseI GACGC 1 cut(s) 518
Csp6I GTAC 2 cut(s) 538, 1328
CspCI CAANNNNNGTGG 2 cut(s) 943, 978
CviAII CATG 9 cut(s) 21, 404, 458, 532, 643, 915, 1255, 1550, 1560
CviQI GTAC 2 cut(s) 538, 1328
DdeI CTNAG 5 cut(s) 46, 870, 895, 1293, 1566
DpnI GATC 7 cut(s) 227, 260, 401, 528, 819, 1250, 1488
DpnII GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1486
DriI GACNNNNNGTC 1 cut(s) 767
EaeI YGGCCR 1 cut(s) 143
Eam1104I CTCTTC 1 cut(s) 1362
Eam1105I GACNNNNNGTC 1 cut(s) 767
EarI CTCTTC 1 cut(s) 1362
Eco130I CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
Eco47I GGWCC 4 cut(s) 472, 761, 859, 1009
Eco57I CTGAAG 1 cut(s) 362
Eco91I GGTNACC 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 707
EcoO65I GGTNACC 1 cut(s) 554
EcoRII CCWGG 3 cut(s) 132, 855, 1228
EcoT14I CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
ErhI CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
FaeI CATG 9 cut(s) 24, 407, 461, 535, 646, 918, 1258, 1553, 1563
FaqI GGGAC 2 cut(s) 521, 1441
FatI CATG 9 cut(s) 20, 403, 457, 531, 642, 914, 1254, 1549, 1559
FauNDI CATATG 2 cut(s) 122, 571
FbaI TGATCA 2 cut(s) 225, 1248
Fnu4HI GCNGC 2 cut(s) 715, 1415
FokI GGATG 4 cut(s) 407, 1091, 1293, 1438
Fsp4HI GCNGC 2 cut(s) 715, 1415
FspBI CTAG 3 cut(s) 102, 773, 1481
FspI TGCGCA 1 cut(s) 127
GlaI GCGC 2 cut(s) 127, 1564
GluI GCNGC 2 cut(s) 715, 1415
HaeIII GGCC 4 cut(s) 145, 352, 708, 755
HapII CCGG 3 cut(s) 711, 759, 765
HgaI GACGC 1 cut(s) 518
HhaI GCGC 2 cut(s) 128, 1565
Hin1II CATG 9 cut(s) 24, 407, 461, 535, 646, 918, 1258, 1553, 1563
Hin6I GCGC 2 cut(s) 126, 1563
HinP1I GCGC 2 cut(s) 126, 1563
HinfI GANTC 5 cut(s) 487, 566, 784, 1429, 1448
HpaII CCGG 3 cut(s) 711, 759, 765
HphI GGTGA 2 cut(s) 512, 947
Hpy166II GTNNAC 5 cut(s) 540, 689, 1048, 1130, 1355
Hpy188I TCNGA 4 cut(s) 263, 873, 1336, 1456
Hpy188III TCNNGA 7 cut(s) 9, 203, 334, 524, 842, 1115, 1463
Hpy8I GTNNAC 5 cut(s) 540, 689, 1048, 1130, 1355
HpyAV CCTTC 4 cut(s) 464, 1201, 1282, 1382
HpyCH4III ACNGT 7 cut(s) 253, 779, 906, 959, 1037, 1174, 1518
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 518, 714, 947, 1312
HpyF3I CTNAG 5 cut(s) 46, 870, 895, 1293, 1566
Hsp92II CATG 9 cut(s) 24, 407, 461, 535, 646, 918, 1258, 1553, 1563
HspAI GCGC 2 cut(s) 126, 1563
Ksp22I TGATCA 2 cut(s) 225, 1248
Kzo9I GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1486
LmnI GCTCC 1 cut(s) 947
Lsp1109I GCAGC 1 cut(s) 1401
LweI GCATC 1 cut(s) 1315
MaeI CTAG 3 cut(s) 102, 773, 1481
MaeIII GTNAC 5 cut(s) 554, 664, 781, 1037, 1234
MalI GATC 7 cut(s) 227, 260, 401, 528, 819, 1250, 1488
MboI GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1486
MboII GAAGA 5 cut(s) 223, 368, 403, 475, 1379
MflI RGATCY 2 cut(s) 399, 1486
MhlI GDGCHC 3 cut(s) 1132, 1167, 1357
MlsI TGGCCA 1 cut(s) 145
MluCI AATT 5 cut(s) 115, 192, 264, 288, 298
MluNI TGGCCA 1 cut(s) 145
MlyI GAGTC 4 cut(s) 575, 778, 1423, 1457
MmeI TCCRAC 3 cut(s) 845, 1284, 1479
MnlI CCTC 6 cut(s) 77, 263, 931, 1064, 1318, 1504
Mox20I TGGCCA 1 cut(s) 145
MscI TGGCCA 1 cut(s) 145
MseI TTAA 2 cut(s) 900, 1089
MslI CAYNNNNRTG 1 cut(s) 1135
Msp20I TGGCCA 1 cut(s) 145
MspA1I CMGCKG 1 cut(s) 1349
MspI CCGG 3 cut(s) 711, 759, 765
MspR9I CCNGG 5 cut(s) 134, 711, 765, 857, 1230
Mva1269I GAATGC 3 cut(s) 27, 242, 454
MvaI CCWGG 3 cut(s) 134, 857, 1230
MwoI GCNNNNNNNGC 5 cut(s) 239, 518, 714, 947, 1312
NciI CCSGG 2 cut(s) 711, 765
NcoI CCATGG 1 cut(s) 914
NdeI CATATG 2 cut(s) 122, 571
NdeII GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1486
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 9 cut(s) 24, 407, 461, 535, 646, 918, 1258, 1553, 1563
NlaIV GGNNCC 5 cut(s) 474, 709, 763, 1488, 1512
NmuCI GTSAC 3 cut(s) 664, 781, 1037
NsbI TGCGCA 1 cut(s) 127
NspI RCATGY 3 cut(s) 24, 1258, 1563
PaeI GCATGC 1 cut(s) 1563
PciI ACATGT 1 cut(s) 1254
PctI GAATGC 3 cut(s) 27, 242, 454
PfeI GAWTC 1 cut(s) 487
PflMI CCANNNNNTGG 2 cut(s) 435, 1391
PkrI GCNGC 2 cut(s) 716, 1416
PleI GAGTC 4 cut(s) 574, 778, 1423, 1456
PpsI GAGTC 4 cut(s) 574, 778, 1423, 1456
PscI ACATGT 1 cut(s) 1254
PsiI TTATAA 2 cut(s) 297, 830
Psp6I CCWGG 3 cut(s) 132, 855, 1228
PspEI GGTNACC 1 cut(s) 554
PspGI CCWGG 3 cut(s) 132, 855, 1228
PspN4I GGNNCC 5 cut(s) 474, 709, 763, 1488, 1512
PspPI GGNCC 6 cut(s) 350, 472, 707, 761, 859, 1009
PstI CTGCAG 3 cut(s) 753, 1055, 1501
PsuI RGATCY 2 cut(s) 399, 1486
PvuII CAGCTG 1 cut(s) 1349
RsaI GTAC 2 cut(s) 539, 1329
RsaNI GTAC 2 cut(s) 538, 1328
RseI CAYNNNNRTG 1 cut(s) 1135
SaqAI TTAA 2 cut(s) 900, 1089
SatI GCNGC 2 cut(s) 715, 1415
Sau3AI GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1486
Sau96I GGNCC 6 cut(s) 350, 472, 707, 761, 859, 1009
SbfI CCTGCAGG 1 cut(s) 753
SchI GAGTC 4 cut(s) 575, 778, 1423, 1457
ScrFI CCNGG 5 cut(s) 134, 711, 765, 857, 1230
SdaI CCTGCAGG 1 cut(s) 753
SduI GDGCHC 3 cut(s) 1132, 1167, 1357
SfaNI GCATC 1 cut(s) 1315
SfcI CTRYAG 4 cut(s) 749, 1033, 1051, 1497
SinI GGWCC 4 cut(s) 472, 761, 859, 1009
SmiMI CAYNNNNRTG 1 cut(s) 1135
SpeI ACTAGT 1 cut(s) 1480
SphI GCATGC 1 cut(s) 1563
Sse8387I CCTGCAGG 1 cut(s) 753
Sse9I AATT 5 cut(s) 115, 192, 264, 288, 298
SsiI CCGC 2 cut(s) 369, 714
SspMI CTAG 3 cut(s) 102, 773, 1481
StyD4I CCNGG 5 cut(s) 132, 709, 763, 855, 1228
StyI CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
TaaI ACNGT 7 cut(s) 253, 779, 906, 959, 1037, 1174, 1518
TaqI TCGA 5 cut(s) 8, 69, 82, 95, 1275
TasI AATT 5 cut(s) 115, 192, 264, 288, 298
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 717
TfiI GAWTC 1 cut(s) 487
Tru1I TTAA 2 cut(s) 900, 1089
Tru9I TTAA 2 cut(s) 900, 1089
TscAI CASTG 3 cut(s) 370, 964, 1510
TseFI GTSAC 3 cut(s) 664, 781, 1037
TseI GCWGC 1 cut(s) 1414
Tsp45I GTSAC 3 cut(s) 664, 781, 1037
TspDTI ATGAA 5 cut(s) 109, 264, 446, 659, 1093
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 3 cut(s) 370, 964, 1510
Van91I CCANNNNNTGG 2 cut(s) 435, 1391
VneI GTGCAC 2 cut(s) 1128, 1353
VpaK11BI GGWCC 4 cut(s) 472, 761, 859, 1009
XapI RAATTY 1 cut(s) 192
XceI RCATGY 3 cut(s) 24, 1258, 1563
XspI CTAG 3 cut(s) 102, 773, 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.