RchiOBHm_Chr4g0438741

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004486
Physical Location & Seq
Forward (+)
61339083 .. 61341828
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40685

Sequence Viewer

Length: 1497 bp
ATGGGGGTTCGAGGAGTGGTTTCGATAGCTAGCACAGATGACAATTTCATATGTGCAACCCTGGATTGGTGGCCAACTGATAAATGTGATTACAATCAATGTCCTTGGGGAAAAGCTGGAATTTTTAATCTGGATTTGAAGAACAAGACCTTGATCAATGCAGTAAAGGCATTCAATACAATAAGGATCAGAATCGGAGGTTCATTGCAAGACCAAATTATTTATAATTTTGGTTATGGAGTGAAAGACTGCAACCAAATCGTGAAAGATGGTTCTGGGCCTTTCTTGTTCAGTGGCGGTTGTCTTCAGCCGAAAAGATGGGATGAGATCCATGAGTTCTTCAACCAAACACAAGCCAAACTCTTGTTTGGATTGAATGCCCTTCATGGGAAGAAAACGGACCCGAAAGACAAGATTCTTTGGGTTGGTGATTGGGACGCAAAAAACGCTCGTGATCTCATGGAGTACACCATCTCAAAGGGTTACCCTGTTGATTCATATGAGCTTGGAAACGAGTTATGTGGGAGTGGGGTAGGTGCAAGAATAGAAGCTGAACAATATGCCAAAGACATGAAAAAGCTCAAACAACTTGTGACAGAGTTGTTCCCAGATGGTAAACAGCCAAAGGTTTTAGGCCCCGGCGGCTTTTATGAGAAAAAGTGGTTCAATGACTTCCTGCAGGCCACCGGACCCGGTGTCCTAGACGGTGTGACTCATCACACTTACGACCTTGGTGCTGGTGTTGATCCAACCCTTATAACCAAGGTTCAAGACCCATTTTACCTGGACCATTCATCTCAGACATATAATGATGTGGCAAACTCAGTTAAACAGTTTGCCCCATGGACAGACCCTTGGGTTGGTGAGGCTGGTGGAGCTTACAACAGTGGTGGCAAAGATGTTTCACATACCTTTGCTAATGGCTTTTGGTATTTGGACCAACTTGGTATGACAGCAAGCTACAGTCACAAGGTTTACTGCAGACAAGCCTTGATTGGAGGGAACTATGCCCTACTTAATACCACATCTTTCATCCCTAATCCTGACTACTATGGTGCACTCCTATGGCATAGACTGATGGGAAATAAAGTGCTCTCTACCGTTCATTATGGCTCTCCCTTCTTGCGTGCATATACTCATTGTTCAAAGAAAAAGCCTGGGGTCACTCTGCTTTTGATCAACATGTCCAACTCTACTACCTTCGATGTTGAAGTTTTCCCCGAGTTCAATCTGCATCCTTGGCAAAGATATGAGGTACAATCGGACACAAAATCAGCTGGTGCACCAATGAGAGAAGAGTATCATTTGACACCAGAAGGTGGCAATATCCAAAGTGATGTGTTGCTACTGAATGGGACTCCATTGAAGCTCACAGAGTCATCCGACATTCCTGAACTGCAACCAAAACTAGTGGATCCTACCTCTGCAGTCAGTGTTGCACCACAGTCGTTCGTCTTTGTATCTATCAAGGACTTCCATGCCCCTGCATGCGCTTAG

Protein Analysis

498

Amino Acids

55.31

Weight (kDa)

6.01

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 3 - 316 1.1e-141 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 225, 758
AccB7I CCANNNNNTGG 1 cut(s) 1319
AciI CCGC 2 cut(s) 297, 642
AclWI GGATC 5 cut(s) 194, 322, 740, 1409, 1422
AcoI YGGCCR 1 cut(s) 71
AcsI RAATTY 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 290
AfaI GTAC 2 cut(s) 467, 1257
AfiI CCNNNNNNNGG 4 cut(s) 66, 387, 860, 1319
AflIII ACRYGT 1 cut(s) 1182
AhdI GACNNNNNGTC 1 cut(s) 695
AhlI ACTAGT 1 cut(s) 1408
AjnI CCWGG 3 cut(s) 60, 783, 1156
AluBI AGCT 9 cut(s) 29, 116, 505, 551, 580, 878, 960, 1277, 1369
AluI AGCT 9 cut(s) 29, 116, 505, 551, 580, 878, 960, 1277, 1369
Alw21I GWGCWC 3 cut(s) 1060, 1095, 1285
Alw44I GTGCAC 2 cut(s) 1056, 1281
AlwI GGATC 5 cut(s) 194, 322, 740, 1409, 1422
Ama87I CYCGRG 1 cut(s) 1220
AoxI GGCC 4 cut(s) 71, 278, 634, 681
ApaLI GTGCAC 2 cut(s) 1056, 1281
ApoI RAATTY 1 cut(s) 120
AspLEI GCGC 1 cut(s) 1493
AspS9I GGNCC 6 cut(s) 278, 400, 635, 689, 787, 937
AsuC2I CCSGG 2 cut(s) 639, 693
AsuHPI GGTGA 2 cut(s) 440, 875
AsuNHI GCTAGC 1 cut(s) 29
AvaI CYCGRG 1 cut(s) 1220
AvaII GGWCC 4 cut(s) 400, 689, 787, 937
BaeGI GKGCMC 2 cut(s) 1060, 1285
BalI TGGCCA 1 cut(s) 73
BamHI GGATCC 1 cut(s) 1414
BauI CACGAG 1 cut(s) 450
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 3 cut(s) 1060, 1095, 1285
BccI CCATC 5 cut(s) 263, 312, 479, 605, 1072
BcgI CGANNNNNNTGC 6 cut(s) 241, 275, 716, 750, 1428, 1462
BciT130I CCWGG 3 cut(s) 62, 785, 1158
BclI TGATCA 2 cut(s) 153, 1176
BcnI CCSGG 2 cut(s) 639, 693
BcuI ACTAGT 1 cut(s) 1408
BfaI CTAG 3 cut(s) 30, 701, 1409
BfmI CTRYAG 4 cut(s) 677, 961, 979, 1425
BglI GCCNNNNNGGC 1 cut(s) 642
BisI GCNGC 1 cut(s) 643
BlsI GCNGC 1 cut(s) 644
Bme1390I CCNGG 5 cut(s) 62, 639, 693, 785, 1158
Bme18I GGWCC 4 cut(s) 400, 689, 787, 937
BmeRI GACNNNNNGTC 1 cut(s) 695
BmeT110I CYCGRG 1 cut(s) 1220
BmgT120I GGNCC 6 cut(s) 278, 400, 635, 689, 787, 937
BmiI GGNNCC 4 cut(s) 402, 637, 691, 1416
BmrFI CCNGG 5 cut(s) 62, 639, 693, 785, 1158
BmsI GCATC 1 cut(s) 1243
BmtI GCTAGC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 296
BpuMI CCSGG 2 cut(s) 639, 693
BsaBI GATNNNNATC 2 cut(s) 93, 191
BsaJI CCNNGG 9 cut(s) 60, 104, 637, 730, 762, 842, 854, 1157, 1238
BsaWI WCCGGW 1 cut(s) 686
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 387, 860, 1319
Bse3DI GCAATG 1 cut(s) 203
Bse8I GATNNNNATC 2 cut(s) 93, 191
BseBI CCWGG 3 cut(s) 62, 785, 1158
BseDI CCNNGG 9 cut(s) 60, 104, 637, 730, 762, 842, 854, 1157, 1238
BseGI GGATG 4 cut(s) 328, 1032, 1234, 1379
BseJI GATNNNNATC 2 cut(s) 93, 191
BseLI CCNNNNNNNGG 4 cut(s) 66, 387, 860, 1319
BseMI GCAATG 1 cut(s) 203
BseMII CTCAG 2 cut(s) 812, 837
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 2 cut(s) 1060, 1285
BshFI GGCC 4 cut(s) 73, 280, 636, 683
BsiHKAI GWGCWC 3 cut(s) 1060, 1095, 1285
BsiHKCI CYCGRG 1 cut(s) 1220
BsiSI CCGG 3 cut(s) 639, 687, 693
BslFI GGGAC 2 cut(s) 449, 1369
BslI CCNNNNNNNGG 4 cut(s) 66, 387, 860, 1319
BsmFI GGGAC 2 cut(s) 449, 1369
BsmI GAATGC 2 cut(s) 170, 382
BsnI GGCC 4 cut(s) 73, 280, 636, 683
BsoBI CYCGRG 1 cut(s) 1220
Bsp1286I GDGCHC 3 cut(s) 1060, 1095, 1285
Bsp143I GATC 7 cut(s) 153, 186, 327, 454, 745, 1176, 1414
Bsp19I CCATGG 1 cut(s) 842
BspACI CCGC 2 cut(s) 297, 642
BspANI GGCC 4 cut(s) 73, 280, 636, 683
BspCNI CTCAG 2 cut(s) 811, 836
BspLI GGNNCC 4 cut(s) 402, 637, 691, 1416
BspMAI CTGCAG 3 cut(s) 681, 983, 1429
BspOI GCTAGC 1 cut(s) 33
BspPI GGATC 5 cut(s) 194, 322, 740, 1409, 1422
BsrDI GCAATG 1 cut(s) 203
BssECI CCNNGG 9 cut(s) 60, 104, 637, 730, 762, 842, 854, 1157, 1238
BssMI GATC 7 cut(s) 153, 186, 327, 454, 745, 1176, 1414
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 6 cut(s) 104, 730, 762, 842, 854, 1238
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 3 cut(s) 62, 785, 1158
Bst4CI ACNGT 6 cut(s) 707, 834, 887, 965, 1102, 1446
Bst6I CTCTTC 1 cut(s) 1290
BstC8I GCNNGC 5 cut(s) 31, 681, 958, 1128, 1489
BstDEI CTNAG 3 cut(s) 798, 823, 1494
BstDSI CCRYGG 1 cut(s) 842
BstEII GGTNACC 1 cut(s) 482
BstF5I GGATG 4 cut(s) 328, 1032, 1234, 1379
BstHHI GCGC 1 cut(s) 1493
BstKTI GATC 7 cut(s) 156, 189, 330, 457, 748, 1179, 1417
BstMBI GATC 7 cut(s) 153, 186, 327, 454, 745, 1176, 1414
BstMWI GCNNNNNNNGC 5 cut(s) 167, 446, 642, 875, 1240
BstNI CCWGG 3 cut(s) 62, 785, 1158
BstNSI RCATGY 2 cut(s) 1186, 1491
BstPI GGTNACC 1 cut(s) 482
BstSCI CCNGG 5 cut(s) 60, 637, 691, 783, 1156
BstSFI CTRYAG 4 cut(s) 677, 961, 979, 1425
BstSLI GKGCMC 2 cut(s) 1060, 1285
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 2 cut(s) 327, 1414
BstYI RGATCY 2 cut(s) 327, 1414
BsuRI GGCC 4 cut(s) 73, 280, 636, 683
BtgI CCRYGG 1 cut(s) 842
BtsCI GGATG 4 cut(s) 328, 1032, 1234, 1379
BtsIMutI CAGTG 3 cut(s) 298, 892, 1438
Cac8I GCNNGC 5 cut(s) 31, 681, 958, 1128, 1489
CfoI GCGC 1 cut(s) 1493
Cfr13I GGNCC 6 cut(s) 278, 400, 635, 689, 787, 937
CseI GACGC 1 cut(s) 446
Csp6I GTAC 2 cut(s) 466, 1256
CspCI CAANNNNNGTGG 4 cut(s) 871, 906, 1392, 1427
CviAII CATG 8 cut(s) 332, 386, 460, 571, 843, 1183, 1478, 1488
CviQI GTAC 2 cut(s) 466, 1256
DdeI CTNAG 3 cut(s) 798, 823, 1494
DpnI GATC 7 cut(s) 155, 188, 329, 456, 747, 1178, 1416
DpnII GATC 7 cut(s) 153, 186, 327, 454, 745, 1176, 1414
DriI GACNNNNNGTC 1 cut(s) 695
EaeI YGGCCR 1 cut(s) 71
Eam1104I CTCTTC 1 cut(s) 1290
Eam1105I GACNNNNNGTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 1290
Eco130I CCWWGG 6 cut(s) 104, 730, 762, 842, 854, 1238
Eco47I GGWCC 4 cut(s) 400, 689, 787, 937
Eco57I CTGAAG 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 1220
Eco91I GGTNACC 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 635
EcoO65I GGTNACC 1 cut(s) 482
EcoRII CCWGG 3 cut(s) 60, 783, 1156
EcoT14I CCWWGG 6 cut(s) 104, 730, 762, 842, 854, 1238
ErhI CCWWGG 6 cut(s) 104, 730, 762, 842, 854, 1238
FaeI CATG 8 cut(s) 335, 389, 463, 574, 846, 1186, 1481, 1491
FaqI GGGAC 2 cut(s) 449, 1369
FatI CATG 8 cut(s) 331, 385, 459, 570, 842, 1182, 1477, 1487
FauNDI CATATG 2 cut(s) 50, 499
FbaI TGATCA 2 cut(s) 153, 1176
Fnu4HI GCNGC 1 cut(s) 643
FokI GGATG 4 cut(s) 335, 1019, 1221, 1366
Fsp4HI GCNGC 1 cut(s) 643
FspBI CTAG 3 cut(s) 30, 701, 1409
GlaI GCGC 1 cut(s) 1492
GluI GCNGC 1 cut(s) 643
HaeIII GGCC 4 cut(s) 73, 280, 636, 683
HapII CCGG 3 cut(s) 639, 687, 693
HgaI GACGC 1 cut(s) 446
HhaI GCGC 1 cut(s) 1493
Hin1II CATG 8 cut(s) 335, 389, 463, 574, 846, 1186, 1481, 1491
Hin6I GCGC 1 cut(s) 1491
HinP1I GCGC 1 cut(s) 1491
HinfI GANTC 6 cut(s) 192, 415, 494, 712, 1357, 1376
HpaII CCGG 3 cut(s) 639, 687, 693
HphI GGTGA 2 cut(s) 440, 875
Hpy166II GTNNAC 5 cut(s) 468, 617, 976, 1058, 1283
Hpy188I TCNGA 5 cut(s) 191, 197, 801, 1264, 1384
Hpy188III TCNNGA 6 cut(s) 131, 262, 452, 770, 1043, 1391
Hpy8I GTNNAC 5 cut(s) 468, 617, 976, 1058, 1283
HpyAV CCTTC 4 cut(s) 392, 1129, 1210, 1310
HpyCH4III ACNGT 6 cut(s) 707, 834, 887, 965, 1102, 1446
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 446, 642, 875, 1240
HpyF3I CTNAG 3 cut(s) 798, 823, 1494
Hsp92II CATG 8 cut(s) 335, 389, 463, 574, 846, 1186, 1481, 1491
HspAI GCGC 1 cut(s) 1491
Ksp22I TGATCA 2 cut(s) 153, 1176
Kzo9I GATC 7 cut(s) 153, 186, 327, 454, 745, 1176, 1414
LmnI GCTCC 1 cut(s) 875
LweI GCATC 1 cut(s) 1243
MaeI CTAG 3 cut(s) 30, 701, 1409
MaeIII GTNAC 5 cut(s) 482, 592, 709, 965, 1162
MalI GATC 7 cut(s) 155, 188, 329, 456, 747, 1178, 1416
MboI GATC 7 cut(s) 153, 186, 327, 454, 745, 1176, 1414
MboII GAAGA 5 cut(s) 151, 296, 331, 403, 1307
MflI RGATCY 2 cut(s) 327, 1414
MhlI GDGCHC 3 cut(s) 1060, 1095, 1285
MlsI TGGCCA 1 cut(s) 73
MluCI AATT 4 cut(s) 43, 120, 216, 226
MluNI TGGCCA 1 cut(s) 73
MlyI GAGTC 3 cut(s) 706, 1351, 1385
MmeI TCCRAC 3 cut(s) 773, 1212, 1407
MnlI CCTC 6 cut(s) 5, 191, 859, 992, 1246, 1432
Mox20I TGGCCA 1 cut(s) 73
MscI TGGCCA 1 cut(s) 73
MseI TTAA 3 cut(s) 126, 828, 1017
MslI CAYNNNNRTG 1 cut(s) 1063
Msp20I TGGCCA 1 cut(s) 73
MspA1I CMGCKG 1 cut(s) 1277
MspI CCGG 3 cut(s) 639, 687, 693
MspR9I CCNGG 5 cut(s) 62, 639, 693, 785, 1158
Mva1269I GAATGC 2 cut(s) 170, 382
MvaI CCWGG 3 cut(s) 62, 785, 1158
MwoI GCNNNNNNNGC 5 cut(s) 167, 446, 642, 875, 1240
NciI CCSGG 2 cut(s) 639, 693
NcoI CCATGG 1 cut(s) 842
NdeI CATATG 2 cut(s) 50, 499
NdeII GATC 7 cut(s) 153, 186, 327, 454, 745, 1176, 1414
NheI GCTAGC 1 cut(s) 29
NlaIII CATG 8 cut(s) 335, 389, 463, 574, 846, 1186, 1481, 1491
NlaIV GGNNCC 4 cut(s) 402, 637, 691, 1416
NmuCI GTSAC 4 cut(s) 592, 709, 965, 1162
NspI RCATGY 2 cut(s) 1186, 1491
PaeI GCATGC 1 cut(s) 1491
PciI ACATGT 1 cut(s) 1182
PctI GAATGC 2 cut(s) 170, 382
PfeI GAWTC 3 cut(s) 192, 415, 494
PflMI CCANNNNNTGG 1 cut(s) 1319
PkrI GCNGC 1 cut(s) 644
PleI GAGTC 3 cut(s) 706, 1351, 1384
PpsI GAGTC 3 cut(s) 706, 1351, 1384
PscI ACATGT 1 cut(s) 1182
PsiI TTATAA 2 cut(s) 225, 758
Psp6I CCWGG 3 cut(s) 60, 783, 1156
PspEI GGTNACC 1 cut(s) 482
PspGI CCWGG 3 cut(s) 60, 783, 1156
PspN4I GGNNCC 4 cut(s) 402, 637, 691, 1416
PspPI GGNCC 6 cut(s) 278, 400, 635, 689, 787, 937
PstI CTGCAG 3 cut(s) 681, 983, 1429
PsuI RGATCY 2 cut(s) 327, 1414
PvuII CAGCTG 1 cut(s) 1277
RsaI GTAC 2 cut(s) 467, 1257
RsaNI GTAC 2 cut(s) 466, 1256
RseI CAYNNNNRTG 1 cut(s) 1063
SaqAI TTAA 3 cut(s) 126, 828, 1017
SatI GCNGC 1 cut(s) 643
Sau3AI GATC 7 cut(s) 153, 186, 327, 454, 745, 1176, 1414
Sau96I GGNCC 6 cut(s) 278, 400, 635, 689, 787, 937
SbfI CCTGCAGG 1 cut(s) 681
SchI GAGTC 3 cut(s) 706, 1351, 1385
ScrFI CCNGG 5 cut(s) 62, 639, 693, 785, 1158
SdaI CCTGCAGG 1 cut(s) 681
SduI GDGCHC 3 cut(s) 1060, 1095, 1285
SfaNI GCATC 1 cut(s) 1243
SfcI CTRYAG 4 cut(s) 677, 961, 979, 1425
SinI GGWCC 4 cut(s) 400, 689, 787, 937
SmiMI CAYNNNNRTG 1 cut(s) 1063
SpeI ACTAGT 1 cut(s) 1408
SphI GCATGC 1 cut(s) 1491
Sse8387I CCTGCAGG 1 cut(s) 681
Sse9I AATT 4 cut(s) 43, 120, 216, 226
SsiI CCGC 2 cut(s) 297, 642
SspMI CTAG 3 cut(s) 30, 701, 1409
StyD4I CCNGG 5 cut(s) 60, 637, 691, 783, 1156
StyI CCWWGG 6 cut(s) 104, 730, 762, 842, 854, 1238
TaaI ACNGT 6 cut(s) 707, 834, 887, 965, 1102, 1446
TaqI TCGA 3 cut(s) 10, 23, 1203
TasI AATT 4 cut(s) 43, 120, 216, 226
TatI WGTACW 1 cut(s) 465
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 3 cut(s) 192, 415, 494
Tru1I TTAA 3 cut(s) 126, 828, 1017
Tru9I TTAA 3 cut(s) 126, 828, 1017
TscAI CASTG 3 cut(s) 298, 892, 1438
TseFI GTSAC 4 cut(s) 592, 709, 965, 1162
Tsp45I GTSAC 4 cut(s) 592, 709, 965, 1162
TspDTI ATGAA 8 cut(s) 37, 192, 374, 486, 587, 783, 1021, 1094
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 3 cut(s) 298, 892, 1438
Van91I CCANNNNNTGG 1 cut(s) 1319
VneI GTGCAC 2 cut(s) 1056, 1281
VpaK11BI GGWCC 4 cut(s) 400, 689, 787, 937
XapI RAATTY 1 cut(s) 120
XceI RCATGY 2 cut(s) 1186, 1491
XspI CTAG 3 cut(s) 30, 701, 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.