Rh4BG375000

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004486
Physical Location & Seq
Forward (+)
53106967 .. 53108062
1096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG375000.1

Sequence Viewer

Length: 768 bp
ATGCGCGCAGGTGTTGATCCAACCCTTATAACCAAGGTTCAAGACCCATTTTACCTGGACCATTCATCTCAGACATATAACGATGTGGCAAACTCAGTTAAACAGTTTGCCCCATGGACAGACCCTTGGGTTGGTGAGGCTGGTGGAGCTTACAACAGTGGTGGCAAAGATGTTTCACATACCTTTGCTAATGGCTTTTGGTATTTGGACCAACTTGGTATGACAGCAAGCTACAGTCACAAGGTTTACTGCAGACAAGCCTTGATTGGAGGGAACTATGCCCTACTTAATACCACGTCTTTCATCCCTAATCCTGACTACTATGGTGCACTGCTATGGCATAGACTGATGGGAAATAAAGTGCTCTCTACCGTTCATTATGGCTCTCCCTTCTTGCGTGCATATACTCATTGTTCAAAGAAAAAGCCTGGGGTCACTCTTCTTTTGATCAACATGTCCAACTCTACTACCTTCGATGTTGAAGTTTTCCCCGAGTTCAATCTGCATCCTTGGCAAAGATATGAGGTACAATCGGACACAAAATCAGCTGGTGCACCAATGAGAGAAGAGTATCATTTGACACCAGAAGGTGGCAATATCCAAAGTGATGTGCTGCTACTGAATGGGACTCCATTGAAGCTCACAGAGTCATCTGACATTCCTGAACTGCAACCAAAACTAGTGGATCCTACCTCTGCAGTCAGTGTTGCACCACAGTCGTTCGTCTTTGTATCTATCAAGGACTTCCATGCCCCTGCATGCGCTTAG

Protein Analysis

255

Amino Acids

28.23

Weight (kDa)

5.95

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 3 - 73 2.6e-27 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 29
AccB7I CCANNNNNTGG 1 cut(s) 590
AccII CGCG 1 cut(s) 6
AclWI GGATC 3 cut(s) 11, 680, 693
AfaI GTAC 1 cut(s) 528
AfiI CCNNNNNNNGG 2 cut(s) 131, 590
AflIII ACRYGT 1 cut(s) 453
AgsI TTSAA 5 cut(s) 41, 417, 482, 499, 637
AhlI ACTAGT 1 cut(s) 679
AjiI CACGTC 1 cut(s) 297
AjnI CCWGG 2 cut(s) 54, 427
AluBI AGCT 4 cut(s) 149, 231, 548, 640
AluI AGCT 4 cut(s) 149, 231, 548, 640
Alw21I GWGCWC 3 cut(s) 331, 366, 556
Alw44I GTGCAC 2 cut(s) 327, 552
AlwI GGATC 3 cut(s) 11, 680, 693
Ama87I CYCGRG 1 cut(s) 491
ApaLI GTGCAC 2 cut(s) 327, 552
ApeKI GCWGC 1 cut(s) 613
AspLEI GCGC 3 cut(s) 6, 8, 764
AspS9I GGNCC 2 cut(s) 58, 208
AsuHPI GGTGA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 491
AvaII GGWCC 2 cut(s) 58, 208
BaeGI GKGCMC 2 cut(s) 331, 556
BamHI GGATCC 1 cut(s) 685
Bbv12I GWGCWC 3 cut(s) 331, 366, 556
BbvI GCAGC 1 cut(s) 600
BccI CCATC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 699, 733
BciT130I CCWGG 2 cut(s) 56, 429
BclI TGATCA 1 cut(s) 447
BcuI ACTAGT 1 cut(s) 679
BfaI CTAG 1 cut(s) 680
BfmI CTRYAG 3 cut(s) 232, 250, 696
BisI GCNGC 1 cut(s) 614
BlsI GCNGC 1 cut(s) 615
Bme1390I CCNGG 2 cut(s) 56, 429
Bme18I GGWCC 2 cut(s) 58, 208
BmeT110I CYCGRG 1 cut(s) 491
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 2 cut(s) 58, 208
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 2 cut(s) 56, 429
BmsI GCATC 1 cut(s) 514
BsaJI CCNNGG 5 cut(s) 33, 113, 125, 428, 509
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 590
BseBI CCWGG 2 cut(s) 56, 429
BseDI CCNNGG 5 cut(s) 33, 113, 125, 428, 509
BseGI GGATG 2 cut(s) 303, 505
BseLI CCNNNNNNNGG 2 cut(s) 131, 590
BseMII CTCAG 2 cut(s) 83, 108
BsePI GCGCGC 1 cut(s) 4
BseSI GKGCMC 2 cut(s) 331, 556
BseXI GCAGC 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 6
BsiHKAI GWGCWC 3 cut(s) 331, 366, 556
BsiHKCI CYCGRG 1 cut(s) 491
BslFI GGGAC 1 cut(s) 640
BslI CCNNNNNNNGG 2 cut(s) 131, 590
BsmFI GGGAC 1 cut(s) 640
BsoBI CYCGRG 1 cut(s) 491
Bsp1286I GDGCHC 3 cut(s) 331, 366, 556
Bsp143I GATC 3 cut(s) 16, 447, 685
Bsp19I CCATGG 1 cut(s) 113
BspCNI CTCAG 2 cut(s) 82, 107
BspFNI CGCG 1 cut(s) 6
BspLI GGNNCC 1 cut(s) 687
BspMAI CTGCAG 2 cut(s) 254, 700
BspPI GGATC 3 cut(s) 11, 680, 693
BssECI CCNNGG 5 cut(s) 33, 113, 125, 428, 509
BssHII GCGCGC 1 cut(s) 4
BssMI GATC 3 cut(s) 16, 447, 685
BssT1I CCWWGG 4 cut(s) 33, 113, 125, 509
Bst2UI CCWGG 2 cut(s) 56, 429
Bst4CI ACNGT 5 cut(s) 105, 158, 236, 373, 717
Bst6I CTCTTC 2 cut(s) 444, 561
BstC8I GCNNGC 4 cut(s) 6, 229, 399, 760
BstDEI CTNAG 3 cut(s) 69, 94, 765
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 2 cut(s) 303, 505
BstFNI CGCG 1 cut(s) 6
BstHHI GCGC 3 cut(s) 6, 8, 764
BstKTI GATC 3 cut(s) 19, 450, 688
BstMBI GATC 3 cut(s) 16, 447, 685
BstMWI GCNNNNNNNGC 2 cut(s) 146, 511
BstNI CCWGG 2 cut(s) 56, 429
BstNSI RCATGY 2 cut(s) 457, 762
BstSCI CCNGG 2 cut(s) 54, 427
BstSFI CTRYAG 3 cut(s) 232, 250, 696
BstSLI GKGCMC 2 cut(s) 331, 556
BstUI CGCG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 600
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BtgI CCRYGG 1 cut(s) 113
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 2 cut(s) 303, 505
BtsI GCAGTG 1 cut(s) 329
BtsIMutI CAGTG 3 cut(s) 163, 329, 709
Cac8I GCNNGC 4 cut(s) 6, 229, 399, 760
CfoI GCGC 3 cut(s) 6, 8, 764
Cfr13I GGNCC 2 cut(s) 58, 208
Csp6I GTAC 1 cut(s) 527
CspCI CAANNNNNGTGG 4 cut(s) 142, 177, 663, 698
CviAII CATG 4 cut(s) 114, 454, 749, 759
CviJI RGCY 9 cut(s) 140, 149, 195, 231, 260, 384, 427, 548, 640
CviKI_1 RGCY 9 cut(s) 140, 149, 195, 231, 260, 384, 427, 548, 640
CviQI GTAC 1 cut(s) 527
DdeI CTNAG 3 cut(s) 69, 94, 765
DpnI GATC 3 cut(s) 18, 449, 687
DpnII GATC 3 cut(s) 16, 447, 685
Eam1104I CTCTTC 2 cut(s) 444, 561
EarI CTCTTC 2 cut(s) 444, 561
Eco130I CCWWGG 4 cut(s) 33, 113, 125, 509
Eco47I GGWCC 2 cut(s) 58, 208
Eco88I CYCGRG 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 54, 427
EcoT14I CCWWGG 4 cut(s) 33, 113, 125, 509
ErhI CCWWGG 4 cut(s) 33, 113, 125, 509
FaeI CATG 4 cut(s) 117, 457, 752, 762
FaqI GGGAC 1 cut(s) 640
FatI CATG 4 cut(s) 113, 453, 748, 758
FbaI TGATCA 1 cut(s) 447
Fnu4HI GCNGC 1 cut(s) 614
FokI GGATG 2 cut(s) 290, 492
Fsp4HI GCNGC 1 cut(s) 614
FspBI CTAG 1 cut(s) 680
GlaI GCGC 3 cut(s) 5, 7, 763
GluI GCNGC 1 cut(s) 614
HhaI GCGC 3 cut(s) 6, 8, 764
Hin1II CATG 4 cut(s) 117, 457, 752, 762
Hin6I GCGC 3 cut(s) 4, 6, 762
HinP1I GCGC 3 cut(s) 4, 6, 762
HinfI GANTC 2 cut(s) 628, 647
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 3 cut(s) 247, 329, 554
Hpy188I TCNGA 3 cut(s) 72, 535, 655
Hpy188III TCNNGA 3 cut(s) 41, 314, 662
Hpy8I GTNNAC 3 cut(s) 247, 329, 554
HpyAV CCTTC 3 cut(s) 400, 481, 581
HpyCH4III ACNGT 5 cut(s) 105, 158, 236, 373, 717
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 9 cut(s) 252, 329, 401, 505, 554, 670, 698, 710, 758
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 511
HpyF3I CTNAG 3 cut(s) 69, 94, 765
HpySE526I ACGT 1 cut(s) 296
Hsp92II CATG 4 cut(s) 117, 457, 752, 762
HspAI GCGC 3 cut(s) 4, 6, 762
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 3 cut(s) 16, 447, 685
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 9 cut(s) 41, 68, 126, 327, 414, 441, 534, 597, 675
Lsp1109I GCAGC 1 cut(s) 600
LweI GCATC 1 cut(s) 514
MaeI CTAG 1 cut(s) 680
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 2 cut(s) 236, 433
MalI GATC 3 cut(s) 18, 449, 687
MboI GATC 3 cut(s) 16, 447, 685
MboII GAAGA 2 cut(s) 431, 578
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 3 cut(s) 331, 366, 556
MlyI GAGTC 2 cut(s) 622, 656
MmeI TCCRAC 2 cut(s) 44, 483
MnlI CCTC 4 cut(s) 130, 263, 517, 703
MseI TTAA 2 cut(s) 99, 288
MslI CAYNNNNRTG 1 cut(s) 334
MspA1I CMGCKG 1 cut(s) 548
MspR9I CCNGG 2 cut(s) 56, 429
MvaI CCWGG 2 cut(s) 56, 429
MvnI CGCG 1 cut(s) 6
MwoI GCNNNNNNNGC 2 cut(s) 146, 511
NcoI CCATGG 1 cut(s) 113
NdeII GATC 3 cut(s) 16, 447, 685
NlaIII CATG 4 cut(s) 117, 457, 752, 762
NlaIV GGNNCC 1 cut(s) 687
NmuCI GTSAC 2 cut(s) 236, 433
NspI RCATGY 2 cut(s) 457, 762
PaeI GCATGC 1 cut(s) 762
PauI GCGCGC 1 cut(s) 4
PciI ACATGT 1 cut(s) 453
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 1 cut(s) 615
PleI GAGTC 2 cut(s) 622, 655
PpsI GAGTC 2 cut(s) 622, 655
PscI ACATGT 1 cut(s) 453
PsiI TTATAA 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 54, 427
PspGI CCWGG 2 cut(s) 54, 427
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 2 cut(s) 58, 208
PstI CTGCAG 2 cut(s) 254, 700
PsuI RGATCY 1 cut(s) 685
PteI GCGCGC 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 1 cut(s) 528
RsaNI GTAC 1 cut(s) 527
RseI CAYNNNNRTG 1 cut(s) 334
SaqAI TTAA 2 cut(s) 99, 288
SatI GCNGC 1 cut(s) 614
Sau3AI GATC 3 cut(s) 16, 447, 685
Sau96I GGNCC 2 cut(s) 58, 208
SchI GAGTC 2 cut(s) 622, 656
ScrFI CCNGG 2 cut(s) 56, 429
SduI GDGCHC 3 cut(s) 331, 366, 556
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 3 cut(s) 232, 250, 696
SinI GGWCC 2 cut(s) 58, 208
SmiMI CAYNNNNRTG 1 cut(s) 334
SpeI ACTAGT 1 cut(s) 679
SphI GCATGC 1 cut(s) 762
SspMI CTAG 1 cut(s) 680
StyD4I CCNGG 2 cut(s) 54, 427
StyI CCWWGG 4 cut(s) 33, 113, 125, 509
TaaI ACNGT 5 cut(s) 105, 158, 236, 373, 717
TaiI ACGT 1 cut(s) 299
TaqI TCGA 1 cut(s) 474
Tru1I TTAA 2 cut(s) 99, 288
Tru9I TTAA 2 cut(s) 99, 288
TscAI CASTG 3 cut(s) 163, 336, 709
TseFI GTSAC 2 cut(s) 236, 433
TseI GCWGC 1 cut(s) 613
Tsp45I GTSAC 2 cut(s) 236, 433
TspDTI ATGAA 3 cut(s) 54, 292, 365
TspRI CASTG 3 cut(s) 163, 336, 709
Van91I CCANNNNNTGG 1 cut(s) 590
VneI GTGCAC 2 cut(s) 327, 552
VpaK11BI GGWCC 2 cut(s) 58, 208
XceI RCATGY 2 cut(s) 457, 762
XspI CTAG 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.