Rw0G007200

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00270
OG0004486
Physical Location & Seq
Forward (+)
78309 .. 80559
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007200.1

Sequence Viewer

Length: 1533 bp
ATGGGGTCGAGAGCTATTGCATGTTGCATTCTGTTGTCATTGTTCTCAGTAGCTTTAGCTGAATATGTCGATGTCAGGGTTCGAGGAGTGGTTTCGATAGCTAGCACAGATGACAATTTCATATGTGCAACCCTGGATTGGTGGCCAACTGATAAATGTGATTACAATCAATGTCCTTGGGGAAAAGCTGGAATTTTTAATCTGGATTTGAAGAACAAGACCTTGATCAATGCAGTAAAGGCATTCAATACAATAAGGATCAGAATTGGAGGTTCATTGCAAGACCAAATTATTTATAATTTTGGTTATGGAGTGAAAGACTGCAACCAAATCGTGAAAGATGGTTCTGGGCCTTTCTTGTTCAGTGGCGGTTGTCTTCAGCCGAAAAGATGGGATGAGATCCATGAGTTCTTCAACCAAACACAAGCCAAACTCTTGTTTGGATTGAATGCCCTTCATGGGAAGAAAACGGACCCGAAAGACAAGATTCTTTGGGTTGGTGATTGGGACGCAAAAAACGCTCGTGATCTCATGGAGTACACCATCTCAAAGGGTTACCCTGTTGAGTCATATGAGCTTGGAAACGAGTTATGTGGGAGTGGGGTAGGTGCAAGAATAGAAGCTGAACAATATGCCAAAGACATGAAAAAGCTCAAACAACTTGTGACAGAGTTGTTCCCAGATGGTAAACAGCCAAAGGTTTTAGGCCCCGGCGGCTTTTATGAGAAAAAGTGGTTCAATGACTTCCTGCAGGCCACCGGACCCGGTGTCCTAGACGGTGTGACTCATCACACTTACGACCTTGGTGCTGGTGTTGATCCAACCCTTATAACCAAGGTTCAAGACCCATTTTACCTGGACCATTCATCTCAGACATATAATGATGTGGCAAACTCAGTTAAACAGTTTGCCCCATGGACAGACCCTTGGGTTGGTGAGGCTGGTGGAGCTTACAACAGTGGTGGCAAAGATGTTTCACATACCTTTGCTAACGGCTTTTGGTATTTGGACCAACTTGGTATGACAGCAAGCTACAGTCACAAGGTTTACTGCAGACAAGCCTTGATTGGAGGGAACTATGCCCTACTTAATACCACATCTTTCATCCCTAATCCTGACTACTATGGTGCACTCCTATGGCATAGACTGATGGGAAATAAAGTGCTCTCTACCGTTCATTATGGCTCTCCCTTCTTGCGTGCATATACTCATTGTTCAAAGAAAAAGCCTGGGGTCACTCTGCTTTTGATCAACATGTCCAACTCTACTACCTTCGATGTTGAAGTTTTCCCCGAGTTCAATCTGCATCCTTGGCAAAGATATGAGGACCAATCGGACACAAAATCAGCTGGTGCACCAATGAGAGAAGAGTATCATTTGACACCAGAAGGTGGCAATATCCAAAGTGATGTGTTGCTACTGAATGGGACTCCATTGAAGCTCACGATGGATCCTACCTCTGCAGTCAGTGTTGCACCACAGTCATTCGTCTTTGTTTCTATCAAGGACTTCCATGCCCCTGCATGCGCTTAG

Protein Analysis

510

Amino Acids

56.63

Weight (kDa)

6.12

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 24 - 340 2.4e-142 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 297, 830
AccB7I CCANNNNNTGG 1 cut(s) 1391
AciI CCGC 2 cut(s) 369, 714
AclWI GGATC 5 cut(s) 266, 394, 812, 1445, 1458
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 4 cut(s) 138, 459, 932, 1391
AflIII ACRYGT 1 cut(s) 1254
AhdI GACNNNNNGTC 1 cut(s) 767
AjnI CCWGG 3 cut(s) 132, 855, 1228
Alw21I GWGCWC 3 cut(s) 1132, 1167, 1357
Alw44I GTGCAC 2 cut(s) 1128, 1353
AlwI GGATC 5 cut(s) 266, 394, 812, 1445, 1458
Ama87I CYCGRG 1 cut(s) 1292
AoxI GGCC 4 cut(s) 143, 350, 706, 753
ApaLI GTGCAC 2 cut(s) 1128, 1353
ApoI RAATTY 1 cut(s) 192
AspLEI GCGC 1 cut(s) 1529
AspS9I GGNCC 7 cut(s) 350, 472, 707, 761, 859, 1009, 1327
AsuC2I CCSGG 2 cut(s) 711, 765
AsuHPI GGTGA 2 cut(s) 512, 947
AsuNHI GCTAGC 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 1292
AvaII GGWCC 5 cut(s) 472, 761, 859, 1009, 1327
BaeGI GKGCMC 2 cut(s) 1132, 1357
BalI TGGCCA 1 cut(s) 145
BamHI GGATCC 1 cut(s) 1450
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 368
Bbv12I GWGCWC 3 cut(s) 1132, 1167, 1357
BccI CCATC 6 cut(s) 335, 384, 551, 677, 1144, 1441
BceAI ACGGC 1 cut(s) 1009
BcgI CGANNNNNNTGC 4 cut(s) 313, 347, 788, 822
BciT130I CCWGG 3 cut(s) 134, 857, 1230
BclI TGATCA 2 cut(s) 225, 1248
BcnI CCSGG 2 cut(s) 711, 765
BfaI CTAG 2 cut(s) 102, 773
BfmI CTRYAG 4 cut(s) 749, 1033, 1051, 1461
BglI GCCNNNNNGGC 1 cut(s) 714
BisI GCNGC 1 cut(s) 715
BlsI GCNGC 1 cut(s) 716
Bme1390I CCNGG 5 cut(s) 134, 711, 765, 857, 1230
Bme18I GGWCC 5 cut(s) 472, 761, 859, 1009, 1327
BmeRI GACNNNNNGTC 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 1292
BmgT120I GGNCC 7 cut(s) 350, 472, 707, 761, 859, 1009, 1327
BmiI GGNNCC 4 cut(s) 474, 709, 763, 1452
BmrFI CCNGG 5 cut(s) 134, 711, 765, 857, 1230
BmsI GCATC 1 cut(s) 1315
BmtI GCTAGC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 368
BpuMI CCSGG 2 cut(s) 711, 765
BsaBI GATNNNNATC 1 cut(s) 165
BsaJI CCNNGG 9 cut(s) 132, 176, 709, 802, 834, 914, 926, 1229, 1310
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 459, 932, 1391
Bse3DI GCAATG 1 cut(s) 275
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 3 cut(s) 134, 857, 1230
BseDI CCNNGG 9 cut(s) 132, 176, 709, 802, 834, 914, 926, 1229, 1310
BseGI GGATG 3 cut(s) 400, 1104, 1306
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 4 cut(s) 138, 459, 932, 1391
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 3 cut(s) 60, 884, 909
BseRI GAGGAG 1 cut(s) 99
BseSI GKGCMC 2 cut(s) 1132, 1357
BshFI GGCC 4 cut(s) 145, 352, 708, 755
BsiHKAI GWGCWC 3 cut(s) 1132, 1167, 1357
BsiHKCI CYCGRG 1 cut(s) 1292
BsiSI CCGG 3 cut(s) 711, 759, 765
BslFI GGGAC 2 cut(s) 521, 1441
BslI CCNNNNNNNGG 4 cut(s) 138, 459, 932, 1391
BsmFI GGGAC 2 cut(s) 521, 1441
BsmI GAATGC 3 cut(s) 27, 242, 454
BsnI GGCC 4 cut(s) 145, 352, 708, 755
BsoBI CYCGRG 1 cut(s) 1292
Bsp1286I GDGCHC 3 cut(s) 1132, 1167, 1357
Bsp143I GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1450
Bsp19I CCATGG 1 cut(s) 914
BspACI CCGC 2 cut(s) 369, 714
BspANI GGCC 4 cut(s) 145, 352, 708, 755
BspCNI CTCAG 3 cut(s) 59, 883, 908
BspLI GGNNCC 4 cut(s) 474, 709, 763, 1452
BspMAI CTGCAG 3 cut(s) 753, 1055, 1465
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 5 cut(s) 266, 394, 812, 1445, 1458
BsrDI GCAATG 1 cut(s) 275
BssECI CCNNGG 9 cut(s) 132, 176, 709, 802, 834, 914, 926, 1229, 1310
BssMI GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1450
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 134, 857, 1230
Bst4CI ACNGT 6 cut(s) 779, 906, 959, 1037, 1174, 1482
Bst6I CTCTTC 1 cut(s) 1362
BstC8I GCNNGC 5 cut(s) 103, 753, 1030, 1200, 1525
BstDEI CTNAG 4 cut(s) 46, 870, 895, 1530
BstDSI CCRYGG 1 cut(s) 914
BstEII GGTNACC 1 cut(s) 554
BstF5I GGATG 3 cut(s) 400, 1104, 1306
BstHHI GCGC 1 cut(s) 1529
BstKTI GATC 7 cut(s) 228, 261, 402, 529, 820, 1251, 1453
BstMBI GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1450
BstMWI GCNNNNNNNGC 5 cut(s) 239, 518, 714, 947, 1312
BstNI CCWGG 3 cut(s) 134, 857, 1230
BstNSI RCATGY 3 cut(s) 24, 1258, 1527
BstPI GGTNACC 1 cut(s) 554
BstSCI CCNGG 5 cut(s) 132, 709, 763, 855, 1228
BstSFI CTRYAG 4 cut(s) 749, 1033, 1051, 1461
BstSLI GKGCMC 2 cut(s) 1132, 1357
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 399, 1450
BstYI RGATCY 2 cut(s) 399, 1450
BsuRI GGCC 4 cut(s) 145, 352, 708, 755
BtgI CCRYGG 1 cut(s) 914
BtsCI GGATG 3 cut(s) 400, 1104, 1306
BtsIMutI CAGTG 3 cut(s) 370, 964, 1474
Cac8I GCNNGC 5 cut(s) 103, 753, 1030, 1200, 1525
CfoI GCGC 1 cut(s) 1529
Cfr13I GGNCC 7 cut(s) 350, 472, 707, 761, 859, 1009, 1327
CseI GACGC 1 cut(s) 518
Csp6I GTAC 1 cut(s) 538
CspCI CAANNNNNGTGG 2 cut(s) 943, 978
CviAII CATG 9 cut(s) 21, 404, 458, 532, 643, 915, 1255, 1514, 1524
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 4 cut(s) 46, 870, 895, 1530
DpnI GATC 7 cut(s) 227, 260, 401, 528, 819, 1250, 1452
DpnII GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1450
DriI GACNNNNNGTC 1 cut(s) 767
EaeI YGGCCR 1 cut(s) 143
Eam1104I CTCTTC 1 cut(s) 1362
Eam1105I GACNNNNNGTC 1 cut(s) 767
EarI CTCTTC 1 cut(s) 1362
Eco130I CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
Eco47I GGWCC 5 cut(s) 472, 761, 859, 1009, 1327
Eco57I CTGAAG 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 1292
Eco91I GGTNACC 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 707
EcoO65I GGTNACC 1 cut(s) 554
EcoRII CCWGG 3 cut(s) 132, 855, 1228
EcoT14I CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
ErhI CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
FaeI CATG 9 cut(s) 24, 407, 461, 535, 646, 918, 1258, 1517, 1527
FaqI GGGAC 2 cut(s) 521, 1441
FatI CATG 9 cut(s) 20, 403, 457, 531, 642, 914, 1254, 1513, 1523
FauNDI CATATG 2 cut(s) 122, 571
FbaI TGATCA 2 cut(s) 225, 1248
Fnu4HI GCNGC 1 cut(s) 715
FokI GGATG 3 cut(s) 407, 1091, 1293
Fsp4HI GCNGC 1 cut(s) 715
FspBI CTAG 2 cut(s) 102, 773
GlaI GCGC 1 cut(s) 1528
GluI GCNGC 1 cut(s) 715
HaeIII GGCC 4 cut(s) 145, 352, 708, 755
HapII CCGG 3 cut(s) 711, 759, 765
HgaI GACGC 1 cut(s) 518
HhaI GCGC 1 cut(s) 1529
Hin1II CATG 9 cut(s) 24, 407, 461, 535, 646, 918, 1258, 1517, 1527
Hin6I GCGC 1 cut(s) 1527
HinP1I GCGC 1 cut(s) 1527
HinfI GANTC 4 cut(s) 487, 566, 784, 1429
HpaII CCGG 3 cut(s) 711, 759, 765
HphI GGTGA 2 cut(s) 512, 947
Hpy166II GTNNAC 5 cut(s) 540, 689, 1048, 1130, 1355
Hpy188I TCNGA 3 cut(s) 263, 873, 1336
Hpy188III TCNNGA 7 cut(s) 9, 203, 334, 524, 842, 1115, 1444
Hpy8I GTNNAC 5 cut(s) 540, 689, 1048, 1130, 1355
HpyAV CCTTC 4 cut(s) 464, 1201, 1282, 1382
HpyCH4III ACNGT 6 cut(s) 779, 906, 959, 1037, 1174, 1482
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 518, 714, 947, 1312
HpyF3I CTNAG 4 cut(s) 46, 870, 895, 1530
Hsp92II CATG 9 cut(s) 24, 407, 461, 535, 646, 918, 1258, 1517, 1527
HspAI GCGC 1 cut(s) 1527
Ksp22I TGATCA 2 cut(s) 225, 1248
Kzo9I GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1450
LmnI GCTCC 1 cut(s) 947
LweI GCATC 1 cut(s) 1315
MaeI CTAG 2 cut(s) 102, 773
MaeIII GTNAC 5 cut(s) 554, 664, 781, 1037, 1234
MalI GATC 7 cut(s) 227, 260, 401, 528, 819, 1250, 1452
MboI GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1450
MboII GAAGA 5 cut(s) 223, 368, 403, 475, 1379
MflI RGATCY 2 cut(s) 399, 1450
MhlI GDGCHC 3 cut(s) 1132, 1167, 1357
MlsI TGGCCA 1 cut(s) 145
MluCI AATT 5 cut(s) 115, 192, 264, 288, 298
MluNI TGGCCA 1 cut(s) 145
MlyI GAGTC 3 cut(s) 575, 778, 1423
MmeI TCCRAC 2 cut(s) 845, 1284
MnlI CCTC 6 cut(s) 77, 263, 931, 1064, 1318, 1468
Mox20I TGGCCA 1 cut(s) 145
MscI TGGCCA 1 cut(s) 145
MseI TTAA 3 cut(s) 198, 900, 1089
MslI CAYNNNNRTG 1 cut(s) 1135
Msp20I TGGCCA 1 cut(s) 145
MspA1I CMGCKG 1 cut(s) 1349
MspI CCGG 3 cut(s) 711, 759, 765
MspR9I CCNGG 5 cut(s) 134, 711, 765, 857, 1230
Mva1269I GAATGC 3 cut(s) 27, 242, 454
MvaI CCWGG 3 cut(s) 134, 857, 1230
MwoI GCNNNNNNNGC 5 cut(s) 239, 518, 714, 947, 1312
NciI CCSGG 2 cut(s) 711, 765
NcoI CCATGG 1 cut(s) 914
NdeI CATATG 2 cut(s) 122, 571
NdeII GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1450
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 9 cut(s) 24, 407, 461, 535, 646, 918, 1258, 1517, 1527
NlaIV GGNNCC 4 cut(s) 474, 709, 763, 1452
NmuCI GTSAC 4 cut(s) 664, 781, 1037, 1234
NspI RCATGY 3 cut(s) 24, 1258, 1527
PaeI GCATGC 1 cut(s) 1527
PciI ACATGT 1 cut(s) 1254
PctI GAATGC 3 cut(s) 27, 242, 454
PfeI GAWTC 1 cut(s) 487
PflMI CCANNNNNTGG 1 cut(s) 1391
PkrI GCNGC 1 cut(s) 716
PleI GAGTC 3 cut(s) 574, 778, 1423
PpsI GAGTC 3 cut(s) 574, 778, 1423
PscI ACATGT 1 cut(s) 1254
PsiI TTATAA 2 cut(s) 297, 830
Psp6I CCWGG 3 cut(s) 132, 855, 1228
PspEI GGTNACC 1 cut(s) 554
PspGI CCWGG 3 cut(s) 132, 855, 1228
PspN4I GGNNCC 4 cut(s) 474, 709, 763, 1452
PspPI GGNCC 7 cut(s) 350, 472, 707, 761, 859, 1009, 1327
PstI CTGCAG 3 cut(s) 753, 1055, 1465
PsuI RGATCY 2 cut(s) 399, 1450
PvuII CAGCTG 1 cut(s) 1349
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 1 cut(s) 1135
SaqAI TTAA 3 cut(s) 198, 900, 1089
SatI GCNGC 1 cut(s) 715
Sau3AI GATC 7 cut(s) 225, 258, 399, 526, 817, 1248, 1450
Sau96I GGNCC 7 cut(s) 350, 472, 707, 761, 859, 1009, 1327
SbfI CCTGCAGG 1 cut(s) 753
SchI GAGTC 3 cut(s) 575, 778, 1423
ScrFI CCNGG 5 cut(s) 134, 711, 765, 857, 1230
SdaI CCTGCAGG 1 cut(s) 753
SduI GDGCHC 3 cut(s) 1132, 1167, 1357
SfaNI GCATC 1 cut(s) 1315
SfcI CTRYAG 4 cut(s) 749, 1033, 1051, 1461
SinI GGWCC 5 cut(s) 472, 761, 859, 1009, 1327
SmiMI CAYNNNNRTG 1 cut(s) 1135
SphI GCATGC 1 cut(s) 1527
Sse8387I CCTGCAGG 1 cut(s) 753
Sse9I AATT 5 cut(s) 115, 192, 264, 288, 298
SsiI CCGC 2 cut(s) 369, 714
SspMI CTAG 2 cut(s) 102, 773
StyD4I CCNGG 5 cut(s) 132, 709, 763, 855, 1228
StyI CCWWGG 6 cut(s) 176, 802, 834, 914, 926, 1310
TaaI ACNGT 6 cut(s) 779, 906, 959, 1037, 1174, 1482
TaqI TCGA 5 cut(s) 8, 69, 82, 95, 1275
TasI AATT 5 cut(s) 115, 192, 264, 288, 298
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 717
TfiI GAWTC 1 cut(s) 487
Tru1I TTAA 3 cut(s) 198, 900, 1089
Tru9I TTAA 3 cut(s) 198, 900, 1089
TscAI CASTG 3 cut(s) 370, 964, 1474
TseFI GTSAC 4 cut(s) 664, 781, 1037, 1234
Tsp45I GTSAC 4 cut(s) 664, 781, 1037, 1234
TspDTI ATGAA 7 cut(s) 109, 264, 446, 659, 855, 1093, 1166
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 3 cut(s) 370, 964, 1474
Van91I CCANNNNNTGG 1 cut(s) 1391
VneI GTGCAC 2 cut(s) 1128, 1353
VpaK11BI GGWCC 5 cut(s) 472, 761, 859, 1009, 1327
XapI RAATTY 1 cut(s) 192
XceI RCATGY 3 cut(s) 24, 1258, 1527
XspI CTAG 2 cut(s) 102, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.