MD16G1063800.v1.1

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004486
Physical Location & Seq
Forward (+)
4520523 .. 4523002
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1063800.v1.1.491

Sequence Viewer

Length: 1527 bp
ATGGAGGTTAGAGGAGGAGCCATATTTTTTATTTGTGTATCGTGGTTTTCATTAGGTTTAGCTGAGTACGTTGAAGTGAGAGTCAAGGGAGTGACTTCCATAGCCACCACAGATGATAACTTCATCTGTGCAACTTTGGACTGGTGGCCTGCTGACAAATGTGACTACGATCAGTGCCCATGGGGGAAGGCTGGCATTTTTAATCTGGATTTGAGTAACAAGATCTTGCACAATGCTGTCAAGGCTTTCAATCCAATGAGAATCAGAGTTGGAGGCTCATTGCAAGATCAGGTCAGTTATGGTGTAGGGTTTGGAGACAAATCATGCAACCGTTTCAAGAAAAATGATTCGGGATTGTTCGGATTCAGCGAAGCTTGCCTTCACCATAAAAGATGGGATGAGCTCAATCAGTTCTTTAACAAAACAAAAGCTAAGGTCACATTTGGTTTGAATGCCTTGCATGGGAAGAAAGTGTCTTCTGAAGACAAAAGTCTTCGAATCGGTGATTGGAACGCTCAAAATTCTCGTGATCTGATGGAGTACACAATTGCAAAGGGATACGAGATTGATTCATATGAGCTTGGGAATGAGCTATCCGGGAGTGGAGTATCTGCAAGAATAGAAAGTGAGCAATATGCAAAAGACATGACAAAGCTCAAAGAAGTTGTGACAGAATTGTACCCCGATGCCTCAAATCGACCTAAGGTTTTAGGCCCTGCTGGATTTTACGACAAGAAATGGTTCGAAAACTTCCTGCAGGCCACGGGGCCAGGCGTTGTTGATGGTCTTATCGACAAGGTTCAAGATCCATTTTTTCTGTCAGAGATAGCTCAAACATTGTACGACTTGTCGAATACAATCCAGCAGTTTGGGCCATGGTCAGAAGCTTGGGTTGGGGAAGCAGGTGGAGCTTATAACAGTGGTGGCAAAGATGTCTCACATACTTTTGCTGATGGCTTTTGGTATTTGGACCAGCTTGGCATGACATCAACCTACAACCACAAGGTTTACTGCCGACAAGCCTTGATTGGAGGGAACTATGCTCTGCTTAACACCACCACATTCATCCCTAATCCCGATTATTACGGTGCACTCTTGTGGCATAGACTAATGGGAACTAGGGTTCTTTCCACCATGAACTTTGGCTCTCCTTATCTGAGAGCATATTGCCATTGCACAAAGAATCAGAAAGGGGTCACACTACTTCTGATCAACATGTCCAACGCCACCACCTTCGACGTCGCTGTTCTCCCCGACTATAATTTGTACCCGAGGCAAATACTTACGACCAGAAAGTACGGTGCTAATGATGAACCAGAAAGAGAAGAGTATCATTTGACACCAGAAGGTGGCAACATTCAAAGTGATGTGGTATTGCTCAATGGAACTCCTTTGAAACTCACAAACTCATCAGACATTCCGGAAATGAAACCAAAACTCGTTGATCCCACTTCGACGATCAGCGTCGCAGCGGATTCAATCGTCTTTGTATCTATAAAAAATTTCCATGCCCCGGCCTGCGCTTAA

Protein Analysis

509

Amino Acids

56.61

Weight (kDa)

5.75

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 24 - 327 4.4e-133 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AarI CACCTGC 1 cut(s) 893
AatII GACGTC 1 cut(s) 1242
Acc36I ACCTGC 1 cut(s) 893
AccB7I CCANNNNNTGG 1 cut(s) 1349
AccIII TCCGGA 1 cut(s) 1420
AciI CCGC 1 cut(s) 1472
AclWI GGATC 2 cut(s) 800, 1439
AcsI RAATTY 2 cut(s) 520, 1501
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 1239
AfaI GTAC 6 cut(s) 68, 542, 680, 842, 1268, 1298
AfiI CCNNNNNNNGG 3 cut(s) 462, 1349, 1513
AflIII ACRYGT 1 cut(s) 1215
AgsI TTSAA 8 cut(s) 74, 250, 337, 451, 803, 1361, 1396, 1479
AjnI CCWGG 1 cut(s) 769
AjuI GAANNNNNNNTTGG 4 cut(s) 490, 522, 876, 908
AleI CACNNNNGTG 1 cut(s) 1096
AloI GAACNNNNNNTCC 2 cut(s) 1107, 1139
Alw21I GWGCWC 2 cut(s) 405, 1093
Alw26I GTCTC 2 cut(s) 309, 940
Alw44I GTGCAC 1 cut(s) 1089
AlwI GGATC 2 cut(s) 800, 1439
Ama87I CYCGRG 1 cut(s) 1270
Aor13HI TCCGGA 1 cut(s) 1420
AoxI GGCC 6 cut(s) 146, 712, 759, 767, 872, 1515
ApaLI GTGCAC 1 cut(s) 1089
ApeKI GCWGC 1 cut(s) 1469
ApoI RAATTY 2 cut(s) 520, 1501
Asp700I GAANNNNTTC 1 cut(s) 740
AspLEI GCGC 1 cut(s) 1523
AspS9I GGNCC 4 cut(s) 713, 767, 872, 970
AsuC2I CCSGG 2 cut(s) 598, 1514
AsuHPI GGTGA 2 cut(s) 374, 515
AsuII TTCGAA 2 cut(s) 496, 744
AvaI CYCGRG 1 cut(s) 1270
AvaII GGWCC 1 cut(s) 970
AxyI CCTNAGG 1 cut(s) 702
BaeGI GKGCMC 2 cut(s) 179, 1093
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 1 cut(s) 525
BbsI GAAGAC 3 cut(s) 468, 485, 489
Bbv12I GWGCWC 2 cut(s) 405, 1093
BbvI GCAGC 1 cut(s) 1481
BccI CCATC 4 cut(s) 387, 529, 776, 947
BciT130I CCWGG 1 cut(s) 771
BciVI GTATCC 1 cut(s) 551
BclI TGATCA 1 cut(s) 1209
BcnI CCSGG 2 cut(s) 598, 1514
BcoDI GTCTC 2 cut(s) 309, 940
BfaI CTAG 1 cut(s) 1119
BfmI CTRYAG 1 cut(s) 755
BfuAI ACCTGC 1 cut(s) 893
BfuI GTATCC 1 cut(s) 551
BglII AGATCT 1 cut(s) 222
BisI GCNGC 1 cut(s) 1470
BlsI GCNGC 1 cut(s) 1471
Bme1390I CCNGG 3 cut(s) 598, 771, 1514
Bme18I GGWCC 1 cut(s) 970
BmeT110I CYCGRG 1 cut(s) 1270
BmgT120I GGNCC 4 cut(s) 713, 767, 872, 970
BmiI GGNNCC 2 cut(s) 19, 768
BmrFI CCNGG 3 cut(s) 598, 771, 1514
BmsI GCATC 1 cut(s) 676
BoxI GACNNNNGTC 1 cut(s) 489
BpiI GAAGAC 3 cut(s) 468, 485, 489
Bpu10I CCTNAGC 1 cut(s) 432
Bpu14I TTCGAA 2 cut(s) 496, 744
BpuMI CCSGG 2 cut(s) 598, 1514
BsaHI GRCGYC 1 cut(s) 1239
BsaJI CCNNGG 5 cut(s) 179, 762, 875, 1271, 1512
BsaWI WCCGGW 1 cut(s) 1420
Bsc4I CCNNNNNNNGG 3 cut(s) 462, 1349, 1513
Bse1I ACTGG 1 cut(s) 146
Bse21I CCTNAGG 1 cut(s) 702
Bse3DI GCAATG 2 cut(s) 278, 1171
BseAI TCCGGA 1 cut(s) 1420
BseBI CCWGG 1 cut(s) 771
BseDI CCNNGG 5 cut(s) 179, 762, 875, 1271, 1512
BseGI GGATG 2 cut(s) 403, 1065
BseLI CCNNNNNNNGG 3 cut(s) 462, 1349, 1513
BseMI GCAATG 2 cut(s) 278, 1171
BseMII CTCAG 2 cut(s) 54, 1148
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 2 cut(s) 27, 30
BseSI GKGCMC 2 cut(s) 179, 1093
BseXI GCAGC 1 cut(s) 1481
BshFI GGCC 6 cut(s) 148, 714, 761, 769, 874, 1517
BsiHKAI GWGCWC 2 cut(s) 405, 1093
BsiHKCI CYCGRG 1 cut(s) 1270
BsiSI CCGG 3 cut(s) 597, 1421, 1514
BslI CCNNNNNNNGG 3 cut(s) 462, 1349, 1513
BsmAI GTCTC 2 cut(s) 309, 940
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 6 cut(s) 148, 714, 761, 769, 874, 1517
BsoBI CYCGRG 1 cut(s) 1270
Bsp119I TTCGAA 2 cut(s) 496, 744
Bsp1286I GDGCHC 3 cut(s) 179, 405, 1093
Bsp13I TCCGGA 1 cut(s) 1420
Bsp143I GATC 8 cut(s) 169, 222, 286, 529, 805, 1209, 1444, 1458
Bsp19I CCATGG 2 cut(s) 179, 875
BspACI CCGC 1 cut(s) 1472
BspANI GGCC 6 cut(s) 148, 714, 761, 769, 874, 1517
BspCNI CTCAG 2 cut(s) 55, 1149
BspEI TCCGGA 1 cut(s) 1420
BspLI GGNNCC 2 cut(s) 19, 768
BspMAI CTGCAG 1 cut(s) 759
BspMI ACCTGC 1 cut(s) 893
BspPI GGATC 2 cut(s) 800, 1439
BspT104I TTCGAA 2 cut(s) 496, 744
BsrDI GCAATG 2 cut(s) 278, 1171
BsrI ACTGG 1 cut(s) 146
BssECI CCNNGG 5 cut(s) 179, 762, 875, 1271, 1512
BssMI GATC 8 cut(s) 169, 222, 286, 529, 805, 1209, 1444, 1458
BssNI GRCGYC 1 cut(s) 1239
BssSI CACGAG 1 cut(s) 525
BssT1I CCWWGG 2 cut(s) 179, 875
Bst2BI CACGAG 1 cut(s) 525
Bst2UI CCWGG 1 cut(s) 771
Bst4CI ACNGT 4 cut(s) 332, 920, 1088, 1301
Bst6I CTCTTC 1 cut(s) 1320
BstACI GRCGYC 1 cut(s) 1239
BstBI TTCGAA 2 cut(s) 496, 744
BstC8I GCNNGC 5 cut(s) 150, 193, 376, 759, 1519
BstDEI CTNAG 4 cut(s) 63, 432, 702, 1157
BstDSI CCRYGG 3 cut(s) 179, 762, 875
BstF5I GGATG 2 cut(s) 403, 1065
BstHHI GCGC 1 cut(s) 1523
BstKTI GATC 8 cut(s) 172, 225, 289, 532, 808, 1212, 1447, 1461
BstMAI GTCTC 2 cut(s) 309, 940
BstMBI GATC 8 cut(s) 169, 222, 286, 529, 805, 1209, 1444, 1458
BstMWI GCNNNNNNNGC 4 cut(s) 242, 375, 871, 908
BstNI CCWGG 1 cut(s) 771
BstNSI RCATGY 1 cut(s) 1219
BstPAI GACNNNNGTC 1 cut(s) 489
BstSCI CCNGG 3 cut(s) 596, 769, 1512
BstSFI CTRYAG 1 cut(s) 755
BstSLI GKGCMC 2 cut(s) 179, 1093
BstV1I GCAGC 1 cut(s) 1481
BstV2I GAAGAC 3 cut(s) 468, 485, 489
BstX2I RGATCY 2 cut(s) 222, 805
BstXI CCANNNNNNTGG 1 cut(s) 869
BstYI RGATCY 2 cut(s) 222, 805
Bsu36I CCTNAGG 1 cut(s) 702
BsuI GTATCC 1 cut(s) 551
BsuRI GGCC 6 cut(s) 148, 714, 761, 769, 874, 1517
BtgI CCRYGG 3 cut(s) 179, 762, 875
BtsCI GGATG 2 cut(s) 403, 1065
BtsIMutI CAGTG 2 cut(s) 179, 925
BveI ACCTGC 1 cut(s) 893
Cac8I GCNNGC 5 cut(s) 150, 193, 376, 759, 1519
CfoI GCGC 1 cut(s) 1523
Cfr13I GGNCC 4 cut(s) 713, 767, 872, 970
CseI GACGC 1 cut(s) 1453
Csp6I GTAC 6 cut(s) 67, 541, 679, 841, 1267, 1297
CviAII CATG 9 cut(s) 180, 324, 461, 646, 876, 982, 1135, 1216, 1508
CviQI GTAC 6 cut(s) 67, 541, 679, 841, 1267, 1297
DdeI CTNAG 4 cut(s) 63, 432, 702, 1157
DpnI GATC 8 cut(s) 171, 224, 288, 531, 807, 1211, 1446, 1460
DpnII GATC 8 cut(s) 169, 222, 286, 529, 805, 1209, 1444, 1458
Eam1104I CTCTTC 1 cut(s) 1320
EarI CTCTTC 1 cut(s) 1320
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 2 cut(s) 179, 875
Eco24I GRGCYC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 970
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 501
Eco81I CCTNAGG 1 cut(s) 702
Eco88I CYCGRG 1 cut(s) 1270
EcoICRI GAGCTC 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 769
EcoT14I CCWWGG 2 cut(s) 179, 875
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 2 cut(s) 179, 875
FaeI CATG 9 cut(s) 183, 327, 464, 649, 879, 985, 1138, 1219, 1511
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FatI CATG 9 cut(s) 179, 323, 460, 645, 875, 981, 1134, 1215, 1507
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 1 cut(s) 1209
Fnu4HI GCNGC 1 cut(s) 1470
FokI GGATG 2 cut(s) 410, 1052
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 1 cut(s) 1470
FspBI CTAG 1 cut(s) 1119
GlaI GCGC 1 cut(s) 1522
GluI GCNGC 1 cut(s) 1470
HaeIII GGCC 6 cut(s) 148, 714, 761, 769, 874, 1517
HapII CCGG 3 cut(s) 597, 1421, 1514
HgaI GACGC 1 cut(s) 1453
HhaI GCGC 1 cut(s) 1523
Hin1I GRCGYC 1 cut(s) 1239
Hin1II CATG 9 cut(s) 183, 327, 464, 649, 879, 985, 1138, 1219, 1511
Hin6I GCGC 1 cut(s) 1521
HinP1I GCGC 1 cut(s) 1521
HindIII AAGCTT 2 cut(s) 372, 885
HinfI GANTC 8 cut(s) 81, 261, 347, 363, 498, 569, 1183, 1475
HpaII CCGG 3 cut(s) 597, 1421, 1514
HphI GGTGA 2 cut(s) 374, 515
Hpy166II GTNNAC 3 cut(s) 543, 1009, 1091
Hpy188III TCNNGA 7 cut(s) 206, 337, 351, 527, 803, 1076, 1421
Hpy8I GTNNAC 3 cut(s) 543, 1009, 1091
Hpy99I CGWCG 4 cut(s) 1241, 1244, 1459, 1469
HpyAV CCTTC 4 cut(s) 181, 389, 1243, 1340
HpyCH4III ACNGT 4 cut(s) 332, 920, 1088, 1301
HpyCH4IV ACGT 2 cut(s) 69, 1239
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 375, 871, 908
HpyF3I CTNAG 4 cut(s) 63, 432, 702, 1157
HpySE526I ACGT 2 cut(s) 69, 1239
Hsp92I GRCGYC 1 cut(s) 1239
Hsp92II CATG 9 cut(s) 183, 327, 464, 649, 879, 985, 1138, 1219, 1511
HspAI GCGC 1 cut(s) 1521
Kpn2I TCCGGA 1 cut(s) 1420
Ksp22I TGATCA 1 cut(s) 1209
Kzo9I GATC 8 cut(s) 169, 222, 286, 529, 805, 1209, 1444, 1458
LmnI GCTCC 2 cut(s) 17, 908
Lsp1109I GCAGC 1 cut(s) 1481
LweI GCATC 1 cut(s) 676
MaeI CTAG 1 cut(s) 1119
MaeII ACGT 2 cut(s) 69, 1239
MaeIII GTNAC 6 cut(s) 91, 161, 215, 436, 667, 1195
MalI GATC 8 cut(s) 171, 224, 288, 531, 807, 1211, 1446, 1460
MboI GATC 8 cut(s) 169, 222, 286, 529, 805, 1209, 1444, 1458
MboII GAAGA 5 cut(s) 468, 478, 485, 494, 1337
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 2 cut(s) 222, 805
MhlI GDGCHC 3 cut(s) 179, 405, 1093
MluCI AATT 5 cut(s) 520, 546, 674, 1261, 1501
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 250, 1245
MnlI CCTC 6 cut(s) 5, 8, 266, 700, 1025, 1266
MroI TCCGGA 1 cut(s) 1420
MroXI GAANNNNTTC 1 cut(s) 740
MseI TTAA 4 cut(s) 201, 417, 1050, 1525
MslI CAYNNNNRTG 1 cut(s) 1096
MspA1I CMGCKG 1 cut(s) 1472
MspI CCGG 3 cut(s) 597, 1421, 1514
MspR9I CCNGG 3 cut(s) 598, 771, 1514
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 1 cut(s) 771
MwoI GCNNNNNNNGC 4 cut(s) 242, 375, 871, 908
NciI CCSGG 2 cut(s) 598, 1514
NcoI CCATGG 2 cut(s) 179, 875
NdeI CATATG 1 cut(s) 574
NdeII GATC 8 cut(s) 169, 222, 286, 529, 805, 1209, 1444, 1458
NlaIII CATG 9 cut(s) 183, 327, 464, 649, 879, 985, 1138, 1219, 1511
NlaIV GGNNCC 2 cut(s) 19, 768
NmuCI GTSAC 5 cut(s) 91, 161, 436, 667, 1195
NspI RCATGY 1 cut(s) 1219
NspV TTCGAA 2 cut(s) 496, 744
OliI CACNNNNGTG 1 cut(s) 1096
PaqCI CACCTGC 1 cut(s) 893
PciI ACATGT 1 cut(s) 1215
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PctI GAATGC 1 cut(s) 457
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 7 cut(s) 261, 347, 363, 498, 569, 1183, 1475
PflMI CCANNNNNTGG 1 cut(s) 1349
PfoI TCCNGGA 1 cut(s) 596
PkrI GCNGC 1 cut(s) 1471
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PscI ACATGT 1 cut(s) 1215
PshAI GACNNNNGTC 1 cut(s) 489
PsiI TTATAA 1 cut(s) 915
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 769
PspGI CCWGG 1 cut(s) 769
PspN4I GGNNCC 2 cut(s) 19, 768
PspPI GGNCC 4 cut(s) 713, 767, 872, 970
PstI CTGCAG 1 cut(s) 759
PsuI RGATCY 2 cut(s) 222, 805
RsaI GTAC 6 cut(s) 68, 542, 680, 842, 1268, 1298
RsaNI GTAC 6 cut(s) 67, 541, 679, 841, 1267, 1297
RseI CAYNNNNRTG 1 cut(s) 1096
SacI GAGCTC 1 cut(s) 405
SaqAI TTAA 4 cut(s) 201, 417, 1050, 1525
SatI GCNGC 1 cut(s) 1470
Sau3AI GATC 8 cut(s) 169, 222, 286, 529, 805, 1209, 1444, 1458
Sau96I GGNCC 4 cut(s) 713, 767, 872, 970
SbfI CCTGCAGG 1 cut(s) 759
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 3 cut(s) 598, 771, 1514
SdaI CCTGCAGG 1 cut(s) 759
SduI GDGCHC 3 cut(s) 179, 405, 1093
SfaNI GCATC 1 cut(s) 676
SfcI CTRYAG 1 cut(s) 755
SfuI TTCGAA 2 cut(s) 496, 744
SinI GGWCC 1 cut(s) 970
SmiMI CAYNNNNRTG 1 cut(s) 1096
Sse8387I CCTGCAGG 1 cut(s) 759
Sse9I AATT 5 cut(s) 520, 546, 674, 1261, 1501
SsiI CCGC 1 cut(s) 1472
SspMI CTAG 1 cut(s) 1119
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 3 cut(s) 596, 769, 1512
StyI CCWWGG 2 cut(s) 179, 875
TaaI ACNGT 4 cut(s) 332, 920, 1088, 1301
TaiI ACGT 2 cut(s) 72, 1242
TaqI TCGA 7 cut(s) 496, 697, 744, 792, 851, 1236, 1454
TasI AATT 5 cut(s) 520, 546, 674, 1261, 1501
TatI WGTACW 1 cut(s) 540
TfiI GAWTC 7 cut(s) 261, 347, 363, 498, 569, 1183, 1475
Tru1I TTAA 4 cut(s) 201, 417, 1050, 1525
Tru9I TTAA 4 cut(s) 201, 417, 1050, 1525
TscAI CASTG 2 cut(s) 179, 925
TseFI GTSAC 5 cut(s) 91, 161, 436, 667, 1195
TseI GCWGC 1 cut(s) 1469
Tsp45I GTSAC 5 cut(s) 91, 161, 436, 667, 1195
TspDTI ATGAA 7 cut(s) 39, 112, 561, 1054, 1151, 1326, 1442
TspRI CASTG 2 cut(s) 179, 925
Van91I CCANNNNNTGG 1 cut(s) 1349
VneI GTGCAC 1 cut(s) 1089
VpaK11BI GGWCC 1 cut(s) 970
XapI RAATTY 2 cut(s) 520, 1501
XceI RCATGY 1 cut(s) 1219
XmnI GAANNNNTTC 1 cut(s) 740
XspI CTAG 1 cut(s) 1119
ZraI GACGTC 1 cut(s) 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.