Rh5BG370100

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004486
Physical Location & Seq
Reverse (-)
58121827 .. 58122935
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG370100.1

Sequence Viewer

Length: 660 bp
ATGGGGTCGAGAGCTATTGCATGTTGCATTCTGTTGTCATTGTTCTCAGTAGCTTTAGTTGAATATGTCGATGTCAGGGATTTGAAGAACAAGACCTTGATCAATGCAGTAAAGGCATTCAATACAATAAGGATAAGAATTGGAGGTTCATTGCAAGACCAAATTATTTATAATTTTGGTTATGGAGTGAAAGACTGCAACCAAATCGTGAAAGATGGTTCTGGGCCTTTCTTGTTCCGTGGCGGTTGTCTTCAGCCGAAAAGATGGGATGAGATCCATGAGTTCTTCAACCAAACACAAGCCAAACTCTTGTTTGGATTGAATGCCCTTCATGGGAAGAAAGCGGACCCTAAAGACAAGATTCTTTGGGTTGGTGATTGGGACGCAAAAAACGCTCGTGATCTCATGGAGTACACCATCTCAAAGGGTTACCCTGCTGAGTCATATGAGCTTGGAAACGAGTTATGTGGGAGTGGGGTAGGTGCAAGAATAGAAGCTGAACAATATGCCAAAGACATGAAAAAGCTCAAACAACTTGTGACAGAGTTGTTCCCAGATGGTAAACAGCCAAAGGTTTTAGGCCCCGGCGGCTCTGAACCCTTATACTCTTTTTTAGTTGTCTTAATCACCTTGTTTAAGTTGCACTCGTGTGCTTCCTAA

Protein Analysis

219

Amino Acids

24.34

Weight (kDa)

8.65

Isoelectric Point (pI)

30.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 27 - 197 6.3e-63 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 171
AciI CCGC 3 cut(s) 243, 344, 588
AclWI GGATC 1 cut(s) 268
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 5 cut(s) 62, 85, 121, 289, 322
AleI CACNNNNGTG 1 cut(s) 648
AluBI AGCT 5 cut(s) 14, 53, 451, 497, 526
AluI AGCT 5 cut(s) 14, 53, 451, 497, 526
AlwI GGATC 1 cut(s) 268
AoxI GGCC 2 cut(s) 224, 580
AspS9I GGNCC 3 cut(s) 224, 346, 581
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 2 cut(s) 386, 619
AvaII GGWCC 1 cut(s) 346
BauI CACGAG 2 cut(s) 396, 646
BbsI GAAGAC 1 cut(s) 242
BccI CCATC 4 cut(s) 209, 258, 425, 551
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BclI TGATCA 1 cut(s) 99
BcnI CCSGG 1 cut(s) 585
BglI GCCNNNNNGGC 1 cut(s) 588
BisI GCNGC 1 cut(s) 589
BlsI GCNGC 1 cut(s) 590
Bme1390I CCNGG 1 cut(s) 585
Bme18I GGWCC 1 cut(s) 346
BmgT120I GGNCC 3 cut(s) 224, 346, 581
BmiI GGNNCC 2 cut(s) 348, 583
BmrFI CCNGG 1 cut(s) 585
BpiI GAAGAC 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 585
BsaJI CCNNGG 2 cut(s) 238, 583
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 238, 583
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 2 cut(s) 60, 429
BshFI GGCC 2 cut(s) 226, 582
BsiSI CCGG 1 cut(s) 585
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 395
BsmI GAATGC 3 cut(s) 27, 116, 328
BsnI GGCC 2 cut(s) 226, 582
Bsp143I GATC 3 cut(s) 99, 273, 400
BspACI CCGC 3 cut(s) 243, 344, 588
BspANI GGCC 2 cut(s) 226, 582
BspCNI CTCAG 2 cut(s) 59, 430
BspLI GGNNCC 2 cut(s) 348, 583
BspPI GGATC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 149
BssECI CCNNGG 2 cut(s) 238, 583
BssMI GATC 3 cut(s) 99, 273, 400
BssSI CACGAG 2 cut(s) 396, 646
Bst2BI CACGAG 2 cut(s) 396, 646
BstDEI CTNAG 2 cut(s) 46, 438
BstDSI CCRYGG 1 cut(s) 238
BstEII GGTNACC 1 cut(s) 428
BstF5I GGATG 1 cut(s) 274
BstKTI GATC 3 cut(s) 102, 276, 403
BstMBI GATC 3 cut(s) 99, 273, 400
BstMWI GCNNNNNNNGC 3 cut(s) 113, 392, 588
BstNSI RCATGY 1 cut(s) 24
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 2 cut(s) 226, 582
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 1 cut(s) 274
Cfr13I GGNCC 3 cut(s) 224, 346, 581
CseI GACGC 1 cut(s) 392
Csp6I GTAC 1 cut(s) 412
CviAII CATG 5 cut(s) 21, 278, 332, 406, 517
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 2 cut(s) 46, 438
DpnI GATC 3 cut(s) 101, 275, 402
DpnII GATC 3 cut(s) 99, 273, 400
Eco47I GGWCC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 236
Eco91I GGTNACC 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 581
EcoO65I GGTNACC 1 cut(s) 428
FaeI CATG 5 cut(s) 24, 281, 335, 409, 520
FaqI GGGAC 1 cut(s) 395
FatI CATG 5 cut(s) 20, 277, 331, 405, 516
FauNDI CATATG 1 cut(s) 445
FbaI TGATCA 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 589
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 1 cut(s) 589
GluI GCNGC 1 cut(s) 589
HaeIII GGCC 2 cut(s) 226, 582
HapII CCGG 1 cut(s) 585
HgaI GACGC 1 cut(s) 392
Hin1II CATG 5 cut(s) 24, 281, 335, 409, 520
HinfI GANTC 2 cut(s) 361, 440
HpaII CCGG 1 cut(s) 585
HphI GGTGA 2 cut(s) 386, 619
Hpy166II GTNNAC 2 cut(s) 414, 563
Hpy188I TCNGA 1 cut(s) 595
Hpy188III TCNNGA 3 cut(s) 9, 208, 398
Hpy8I GTNNAC 2 cut(s) 414, 563
HpyAV CCTTC 1 cut(s) 338
HpyCH4V TGCA 7 cut(s) 20, 27, 107, 154, 198, 485, 643
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 392, 588
HpyF3I CTNAG 2 cut(s) 46, 438
Hsp92II CATG 5 cut(s) 24, 281, 335, 409, 520
Ksp22I TGATCA 1 cut(s) 99
Kzo9I GATC 3 cut(s) 99, 273, 400
LpnPI CCDG 5 cut(s) 61, 207, 447, 567, 598
MaeIII GTNAC 2 cut(s) 428, 538
MalI GATC 3 cut(s) 101, 275, 402
MboI GATC 3 cut(s) 99, 273, 400
MboII GAAGA 4 cut(s) 97, 242, 277, 349
MflI RGATCY 1 cut(s) 273
MluCI AATT 3 cut(s) 138, 162, 172
MlyI GAGTC 1 cut(s) 449
MnlI CCTC 1 cut(s) 137
MseI TTAA 2 cut(s) 623, 636
MslI CAYNNNNRTG 1 cut(s) 648
MspI CCGG 1 cut(s) 585
MspR9I CCNGG 1 cut(s) 585
Mva1269I GAATGC 3 cut(s) 27, 116, 328
MwoI GCNNNNNNNGC 3 cut(s) 113, 392, 588
NciI CCSGG 1 cut(s) 585
NdeI CATATG 1 cut(s) 445
NdeII GATC 3 cut(s) 99, 273, 400
NlaIII CATG 5 cut(s) 24, 281, 335, 409, 520
NlaIV GGNNCC 2 cut(s) 348, 583
NmuCI GTSAC 1 cut(s) 538
NspI RCATGY 1 cut(s) 24
OliI CACNNNNGTG 1 cut(s) 648
PctI GAATGC 3 cut(s) 27, 116, 328
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 1 cut(s) 590
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PsiI TTATAA 1 cut(s) 171
PspEI GGTNACC 1 cut(s) 428
PspN4I GGNNCC 2 cut(s) 348, 583
PspPI GGNCC 3 cut(s) 224, 346, 581
PsuI RGATCY 1 cut(s) 273
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 648
SaqAI TTAA 2 cut(s) 623, 636
SatI GCNGC 1 cut(s) 589
Sau3AI GATC 3 cut(s) 99, 273, 400
Sau96I GGNCC 3 cut(s) 224, 346, 581
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 585
SinI GGWCC 1 cut(s) 346
SmiMI CAYNNNNRTG 1 cut(s) 648
Sse9I AATT 3 cut(s) 138, 162, 172
SsiI CCGC 3 cut(s) 243, 344, 588
StyD4I CCNGG 1 cut(s) 583
TaqI TCGA 2 cut(s) 8, 69
TasI AATT 3 cut(s) 138, 162, 172
TatI WGTACW 1 cut(s) 411
TauI GCSGC 1 cut(s) 591
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 2 cut(s) 623, 636
Tru9I TTAA 2 cut(s) 623, 636
TseFI GTSAC 1 cut(s) 538
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 3 cut(s) 138, 320, 533
TspGWI ACGGA 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 346
XceI RCATGY 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.