AT5G61450

phosphotransferase activity, carboxyl group as acceptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002381
Physical Location & Seq
Reverse (-)
24709862 .. 24713010
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61450.1

Sequence Viewer

Length: 1344 bp
ATGAAGGACAGAGAGGAATCATCATCGTCTTCTTCTTCAATGACAACAAAAAAAACTAATGGAATAATCAACAACGGCGAACACGAACCTCGAGATATCAACATCCGATCATTCTCTTCTGTTCCTTCTTCTCCTCGTAACGCTTCTTCCAAATACGATTTCGTCAAGGTCAAAGTATGGCTTGGAGACAACGCAGATCACTACTATGTTCTGTCTCGATTCTTAGTCTGTAGAATGTTAACTGTTACTAAGATCCCAAATCATGAAGCTATAAAGATTTCTCTTGAACTCAAGAAGCTTCTAATTGACAACAGTCTTCTTGATGTCTCTCAGTCTGATTTGGAAACCAATTTGTTTAAGCTAATGGAGCGACGTGGTTATGGAGAAGAGTACATTAATCGTTATAACATGATGACAAAATTTCATCATCAAAGAGTACCTCTTGTCATACTCGTTTGCGGAACAGCTTGTGTTGGCAAGTCCACTATAGCTACACAACTTGCTCAGAGACTTAACTTGCCAAACGTTTTACAAACAGACATGGTTTATGAGCTGCTTCGAACAGCAACTGATGCTCCGTTGACATCAACCCCTGTATGGACTCGAGAATTCGGCTCATCAGAAGAGTTGATTACTGAATTCTGTAGAGAATGCAGAATTGTTCGGAAAGGACTTGGTGGTGATTTGAAGAAAGCAATGAAAGATGGGAAACCCATTATAATTGAGGGAAGACATTTAGATCCAAGCATATACTTAATGAATGATGAGAACAAAACTCCATCAAATGATCCCGAGAAGAGCAGCAGCGAAACAAACTCTTCGAAAGATCTAACCTCGGACAAAAATCCCGAGGCAAGTTCTTCAAACACGAAAGAAACCGACAATAGTGCTGTGAAACCCCATTCTCATGAGGAAGCTGTGGCATCTGTAGAAGCGAACAACCTCTCAGAAAAAGTTACACAATGTACAATCGACGCTGAAAGTTCAAAAGATGCTGAAAAGAAAAGCAAATCTGCAGATGGAAGTGGAAAGACGAAATCTGGCCCTGAACCAATAGTCATATCTATTGTGTTAAAGATGTCTGAATTCGATCATAAGGCTTTGCTAGAGGAGTGGATCTCTTCCCGTACATCTGGAGAGAAATATACCACTAAGGAGAAAGATAGACTAATAACAAACCTAAAGACCATTGAAGACTACCTTTGTTCCTTTAATTCACAGGGGGTGACTGTTGTAAATATATCGGCCACCACATTCCCACAGACACTAGATTGGCTTCACAACTATCTTCTTCAGCGTATTGAGGAAGGGATTCGATCATCAGAGAACGAGGCTCCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_18

Protein Analysis

447

Amino Acids

50.07

Weight (kDa)

6.13

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_18 PF13238 150 - 248 1.8e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 405, 719
AciI CCGC 1 cut(s) 459
AclI AACGTT 1 cut(s) 525
AclWI GGATC 4 cut(s) 247, 734, 782, 1124
AcoI YGGCCR 1 cut(s) 1245
AcsI RAATTY 4 cut(s) 419, 608, 638, 1085
AcuI CTGAAG 1 cut(s) 1277
AfaI GTAC 4 cut(s) 392, 438, 967, 1129
AfiI CCNNNNNNNGG 1 cut(s) 597
AgsI TTSAA 6 cut(s) 39, 287, 688, 864, 987, 1193
AjiI CACGTC 1 cut(s) 374
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 7 cut(s) 269, 298, 361, 467, 491, 553, 917
AluI AGCT 7 cut(s) 269, 298, 361, 467, 491, 553, 917
Alw26I GTCTC 4 cut(s) 180, 219, 331, 502
AlwI GGATC 4 cut(s) 247, 734, 782, 1124
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 4 cut(s) 90, 603, 791, 848
AoxI GGCC 2 cut(s) 1042, 1245
ApeKI GCWGC 3 cut(s) 553, 801, 804
ApoI RAATTY 4 cut(s) 419, 608, 638, 1085
AseI ATTAAT 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 1311
AspS9I GGNCC 1 cut(s) 1043
AsuHPI GGTGA 2 cut(s) 692, 1237
AsuII TTCGAA 2 cut(s) 559, 821
AvaI CYCGRG 4 cut(s) 90, 603, 791, 848
BarI GAAGNNNNNNTAC 2 cut(s) 130, 162
BbsI GAAGAC 4 cut(s) 21, 308, 736, 1200
BbvI GCAGC 3 cut(s) 540, 813, 816
BccI CCATC 3 cut(s) 698, 787, 1013
BceAI ACGGC 1 cut(s) 91
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BcoDI GTCTC 4 cut(s) 180, 219, 331, 502
BfaI CTAG 2 cut(s) 1106, 1268
BfmI CTRYAG 5 cut(s) 229, 486, 643, 927, 1014
BglII AGATCT 1 cut(s) 826
BisI GCNGC 3 cut(s) 554, 802, 805
BlsI GCNGC 3 cut(s) 555, 803, 806
BmeT110I CYCGRG 4 cut(s) 90, 603, 791, 848
BmgBI CACGTC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 1043
BmiI GGNNCC 1 cut(s) 1335
BmsI GCATC 3 cut(s) 562, 932, 982
BoxI GACNNNNGTC 1 cut(s) 312
BpiI GAAGAC 4 cut(s) 21, 308, 736, 1200
BplI GAGNNNNNCTC 2 cut(s) 1103, 1135
BpmI CTGGAG 1 cut(s) 1155
Bpu14I TTCGAA 2 cut(s) 559, 821
BpuEI CTTGAG 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 834, 849
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 1 cut(s) 597
Bse3DI GCAATG 1 cut(s) 702
BseDI CCNNGG 2 cut(s) 834, 849
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 597
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 3 cut(s) 344, 518, 960
BseRI GAGGAG 2 cut(s) 123, 1124
BseXI GCAGC 3 cut(s) 540, 813, 816
BshFI GGCC 2 cut(s) 1044, 1247
BsiHKCI CYCGRG 4 cut(s) 90, 603, 791, 848
BslI CCNNNNNNNGG 1 cut(s) 597
BsmAI GTCTC 4 cut(s) 180, 219, 331, 502
BsmI GAATGC 1 cut(s) 656
BsnI GGCC 2 cut(s) 1044, 1247
BsoBI CYCGRG 4 cut(s) 90, 603, 791, 848
Bsp119I TTCGAA 2 cut(s) 559, 821
Bsp1407I TGTACA 1 cut(s) 965
Bsp143I GATC 9 cut(s) 107, 196, 252, 739, 787, 826, 1090, 1116, 1316
BspACI CCGC 1 cut(s) 459
BspANI GGCC 2 cut(s) 1044, 1247
BspCNI CTCAG 3 cut(s) 343, 517, 959
BspHI TCATGA 2 cut(s) 262, 907
BspLI GGNNCC 1 cut(s) 1335
BspMAI CTGCAG 1 cut(s) 1018
BspPI GGATC 4 cut(s) 247, 734, 782, 1124
BspQI GCTCTTC 1 cut(s) 791
BspT104I TTCGAA 2 cut(s) 559, 821
BsrDI GCAATG 1 cut(s) 702
BsrGI TGTACA 1 cut(s) 965
BssECI CCNNGG 2 cut(s) 834, 849
BssMI GATC 9 cut(s) 107, 196, 252, 739, 787, 826, 1090, 1116, 1316
Bst4CI ACNGT 3 cut(s) 244, 314, 1231
Bst6I CTCTTC 6 cut(s) 121, 381, 618, 791, 823, 1126
BstAPI GCANNNNNTGC 1 cut(s) 572
BstAUI TGTACA 1 cut(s) 965
BstBI TTCGAA 2 cut(s) 559, 821
BstDEI CTNAG 6 cut(s) 223, 249, 330, 504, 946, 1152
BstF5I GGATG 1 cut(s) 102
BstKTI GATC 9 cut(s) 110, 199, 255, 742, 790, 829, 1093, 1119, 1319
BstMAI GTCTC 4 cut(s) 180, 219, 331, 502
BstMBI GATC 9 cut(s) 107, 196, 252, 739, 787, 826, 1090, 1116, 1316
BstMWI GCNNNNNNNGC 2 cut(s) 367, 572
BstPAI GACNNNNGTC 1 cut(s) 312
BstSFI CTRYAG 5 cut(s) 229, 486, 643, 927, 1014
BstV1I GCAGC 3 cut(s) 540, 813, 816
BstV2I GAAGAC 4 cut(s) 21, 308, 736, 1200
BstX2I RGATCY 4 cut(s) 252, 739, 826, 1116
BstYI RGATCY 4 cut(s) 252, 739, 826, 1116
BsuRI GGCC 2 cut(s) 1044, 1247
BtrI CACGTC 1 cut(s) 374
BtsCI GGATG 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 2 cut(s) 262, 907
Cfr13I GGNCC 1 cut(s) 1043
CseI GACGC 1 cut(s) 983
Csp6I GTAC 4 cut(s) 391, 437, 966, 1128
CviAII CATG 4 cut(s) 263, 409, 541, 908
CviQI GTAC 4 cut(s) 391, 437, 966, 1128
DdeI CTNAG 6 cut(s) 223, 249, 330, 504, 946, 1152
DpnI GATC 9 cut(s) 109, 198, 254, 741, 789, 828, 1092, 1118, 1318
DpnII GATC 9 cut(s) 107, 196, 252, 739, 787, 826, 1090, 1116, 1316
EaeI YGGCCR 1 cut(s) 1245
Eam1104I CTCTTC 6 cut(s) 121, 381, 618, 791, 823, 1126
EarI CTCTTC 6 cut(s) 121, 381, 618, 791, 823, 1126
Eco32I GATATC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 1277
Eco88I CYCGRG 4 cut(s) 90, 603, 791, 848
EcoRI GAATTC 3 cut(s) 608, 638, 1085
EcoRV GATATC 1 cut(s) 97
FaeI CATG 4 cut(s) 266, 412, 544, 911
FalI AAGNNNNNCTT 4 cut(s) 165, 197, 1185, 1217
FatI CATG 4 cut(s) 262, 408, 540, 907
Fnu4HI GCNGC 3 cut(s) 554, 802, 805
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 3 cut(s) 554, 802, 805
FspBI CTAG 2 cut(s) 1106, 1268
GluI GCNGC 3 cut(s) 554, 802, 805
GsuI CTGGAG 1 cut(s) 1155
HaeIII GGCC 2 cut(s) 1044, 1247
HgaI GACGC 1 cut(s) 983
Hin1II CATG 4 cut(s) 266, 412, 544, 911
HincII GTYRAC 2 cut(s) 240, 582
HindII GTYRAC 2 cut(s) 240, 582
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 4 cut(s) 17, 219, 601, 1312
HpaI GTTAAC 1 cut(s) 240
HphI GGTGA 2 cut(s) 692, 1237
Hpy166II GTNNAC 3 cut(s) 240, 483, 582
Hpy8I GTNNAC 3 cut(s) 240, 483, 582
Hpy99I CGWCG 2 cut(s) 375, 977
HpyAV CCTTC 2 cut(s) 135, 1301
HpyCH4III ACNGT 3 cut(s) 244, 314, 1231
HpyCH4IV ACGT 2 cut(s) 373, 525
HpyCH4V TGCA 2 cut(s) 654, 1016
HpyF10VI GCNNNNNNNGC 2 cut(s) 367, 572
HpyF3I CTNAG 6 cut(s) 223, 249, 330, 504, 946, 1152
HpySE526I ACGT 2 cut(s) 373, 525
Hsp92II CATG 4 cut(s) 266, 412, 544, 911
KspAI GTTAAC 1 cut(s) 240
Kzo9I GATC 9 cut(s) 107, 196, 252, 739, 787, 826, 1090, 1116, 1316
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 3 cut(s) 367, 580, 1339
LpnPI CCDG 5 cut(s) 606, 1026, 1059, 1119, 1205
Lsp1109I GCAGC 3 cut(s) 540, 813, 816
LweI GCATC 3 cut(s) 562, 932, 982
MaeI CTAG 2 cut(s) 1106, 1268
MaeII ACGT 2 cut(s) 373, 525
MaeIII GTNAC 4 cut(s) 137, 244, 955, 1225
MalI GATC 9 cut(s) 109, 198, 254, 741, 789, 828, 1092, 1118, 1318
MboI GATC 9 cut(s) 107, 196, 252, 739, 787, 826, 1090, 1116, 1316
MflI RGATCY 4 cut(s) 252, 739, 826, 1116
MluCI AATT 9 cut(s) 303, 349, 419, 608, 638, 657, 720, 1085, 1213
MlyI GAGTC 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 1311
MseI TTAA 7 cut(s) 239, 357, 396, 513, 755, 1073, 1212
MslI CAYNNNNRTG 2 cut(s) 204, 906
Mva1269I GAATGC 1 cut(s) 656
MwoI GCNNNNNNNGC 2 cut(s) 367, 572
NdeII GATC 9 cut(s) 107, 196, 252, 739, 787, 826, 1090, 1116, 1316
NlaIII CATG 4 cut(s) 266, 412, 544, 911
NlaIV GGNNCC 1 cut(s) 1335
NmuCI GTSAC 1 cut(s) 1225
NspV TTCGAA 2 cut(s) 559, 821
PaeR7I CTCGAG 2 cut(s) 90, 603
PagI TCATGA 2 cut(s) 262, 907
PciSI GCTCTTC 1 cut(s) 791
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 656
PdmI GAANNNNTTC 1 cut(s) 1311
PfeI GAWTC 3 cut(s) 17, 219, 1312
PkrI GCNGC 3 cut(s) 555, 803, 806
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
PshAI GACNNNNGTC 1 cut(s) 312
PshBI ATTAAT 1 cut(s) 396
PsiI TTATAA 2 cut(s) 405, 719
Psp1406I AACGTT 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 1335
PspPI GGNCC 1 cut(s) 1043
PstI CTGCAG 1 cut(s) 1018
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 4 cut(s) 252, 739, 826, 1116
RsaI GTAC 4 cut(s) 392, 438, 967, 1129
RsaNI GTAC 4 cut(s) 391, 437, 966, 1128
RseI CAYNNNNRTG 2 cut(s) 204, 906
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 7 cut(s) 239, 357, 396, 513, 755, 1073, 1212
SatI GCNGC 3 cut(s) 554, 802, 805
Sau3AI GATC 9 cut(s) 107, 196, 252, 739, 787, 826, 1090, 1116, 1316
Sau96I GGNCC 1 cut(s) 1043
SchI GAGTC 1 cut(s) 595
SfaNI GCATC 3 cut(s) 562, 932, 982
SfcI CTRYAG 5 cut(s) 229, 486, 643, 927, 1014
Sfr274I CTCGAG 2 cut(s) 90, 603
SfuI TTCGAA 2 cut(s) 559, 821
SlaI CTCGAG 2 cut(s) 90, 603
SmiMI CAYNNNNRTG 2 cut(s) 204, 906
SmlI CTYRAG 3 cut(s) 90, 290, 603
SmoI CTYRAG 3 cut(s) 90, 290, 603
Sse9I AATT 9 cut(s) 303, 349, 419, 608, 638, 657, 720, 1085, 1213
SsiI CCGC 1 cut(s) 459
SspMI CTAG 2 cut(s) 1106, 1268
TaaI ACNGT 3 cut(s) 244, 314, 1231
TaiI ACGT 2 cut(s) 376, 528
TaqI TCGA 8 cut(s) 91, 217, 559, 604, 821, 972, 1089, 1315
TasI AATT 9 cut(s) 303, 349, 419, 608, 638, 657, 720, 1085, 1213
TatI WGTACW 2 cut(s) 390, 965
TfiI GAWTC 3 cut(s) 17, 219, 1312
Tru1I TTAA 7 cut(s) 239, 357, 396, 513, 755, 1073, 1212
Tru9I TTAA 7 cut(s) 239, 357, 396, 513, 755, 1073, 1212
TseFI GTSAC 1 cut(s) 1225
TseI GCWGC 3 cut(s) 553, 801, 804
Tsp45I GTSAC 1 cut(s) 1225
TspDTI ATGAA 5 cut(s) 17, 279, 413, 713, 773
TspGWI ACGGA 1 cut(s) 567
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 4 cut(s) 419, 608, 638, 1085
XhoI CTCGAG 2 cut(s) 90, 603
XmnI GAANNNNTTC 1 cut(s) 1311
XspI CTAG 2 cut(s) 1106, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.