MD07G1252500.v1.1

phosphotransferase activity, carboxyl group as acceptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002381
Physical Location & Seq
Forward (+)
32029544 .. 32034376
4833 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1252500.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGGGATTCCACCCGGACACACAGACGATGAACAACAAAGGCAAAGCAGTCGCCGTCTCAAGCCCCAACATCAACGACATCGACGACGACAACGTTAACATCGGCGTAGACGACTTGGATTTGGACGACGACAACAACCACAGAAGTAGTGGCGTCTCTTCGACTTCCATCAGATTCTCGTCCCGCAACACTTCCTCCAAATACGACTTTGTCAAGGTGAAGGTCTGGCTGGGTGATAATGCAGATCACTACTATGTTCTTTCCAGATTTTTGCTCAGTCGAATGTTGACTGTCACTAAGATCCCGAATTATGTAGCGATTAAAATCGCGCTGGAGCTCAAGAAGCTGCTGATTGACAACAGCCTGCTAGATGTTTCGCAGTCCGATTTGGAAGTGAATTTGTTTAAGCTTATGGAACGAAGGGGTTATGGGGAAGAGTACATAAATCGGTACAAGATGATGACCAGATTTCACCATCAACGAGTACCATTGGTTATTCTTGTATGTGGAACTGCCTGTGTTGGAAAGTCGACCATTGCTACCCAACTTGCACAAAGACTAAATTTGCCTAATGTCTTGCAGACAGATATGGTATATGAATTGTTGCGCACGTCAACAGATGCACCATTGACATCTACTCCTGTATGGGAACGAGACTTCAGCTCCTCCGAGGAGTTAATTACTGAATTTTGTAGGGAATGCAGGATTGTCCGTAAAGGATTGGCTGGTGATTTGAAGAAAGCTATGAAAGATGGAAAGCCAATAATAATAGAGGGAATACATTTGGATCCAAGCATTTATTTAATGGAAGATGATAATAAAACACAAGCACAAGAGAAAACTCAGGAGGCAGACCCAGTTGCAGTAGATGGTACCAAAACACAGATGGAAAACAGTTCTCCAATTATAAGCGGAAGTTCTTCTGAAGTTTTCAACAGTGCTGCACATGTCAGCTGCGAAGAAGGGACACCAACTAACAAGGTGGACAAAGTCTTGGACCACCTGGAAGCTATTGACCTAGCCGGAAATGTTTCTGAGGAAAAAGGTGAAAGTGTTACTGATCCAGAGTTGAGTAAACGTACTTCTGTTAAGAAAGAGAAGTCTGGTACCGAACCAATTATTATACCTCTAGTTTTGAAGATGGCTGAGTTTGATCATAAGGCATTACTAGAAGAGGTGATCTCGACTCGTACATTTAGTGATAAATCTATAATCCAGGATAAAGATAAGCTAATACGCAACTTAAAGACCATCCAGGATTATCTTTGTTCATTCAATTCGCAGGGATTGACAGTCGTCAATATATCATCAACCACATTTCCGCAAACACTGGATTGGCTGCATGGGTATCTTCTTGAGTGTATAGAGCATGGTATTTCATCAGTGTCCAATGGAAATAGTAGGCGGCCTGCGGAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_18

Protein Analysis

474

Amino Acids

52.79

Weight (kDa)

5.3

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_18 PF13238 166 - 269 2.4e-07 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 908
Acc16I TGCGCA 1 cut(s) 608
Acc65I GGTACC 2 cut(s) 872, 1106
AccB1I GGYRCC 2 cut(s) 872, 1106
AccI GTMKAC 2 cut(s) 108, 530
AccII CGCG 1 cut(s) 329
AciI CCGC 5 cut(s) 184, 912, 1322, 1405, 1412
AclI AACGTT 1 cut(s) 93
AclWI GGATC 4 cut(s) 295, 782, 795, 1055
AcsI RAATTY 3 cut(s) 397, 562, 686
AcuI CTGAAG 2 cut(s) 643, 945
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 7 cut(s) 440, 452, 486, 874, 1081, 1108, 1192
AflIII ACRYGT 1 cut(s) 946
AgsI TTSAA 4 cut(s) 736, 934, 1138, 1276
AjiI CACGTC 1 cut(s) 612
AjnI CCWGG 3 cut(s) 1002, 1215, 1254
AluBI AGCT 8 cut(s) 337, 346, 409, 663, 743, 954, 1010, 1231
AluI AGCT 8 cut(s) 337, 346, 409, 663, 743, 954, 1010, 1231
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 3 cut(s) 61, 160, 648
AlwI GGATC 4 cut(s) 295, 782, 795, 1055
AoxI GGCC 1 cut(s) 1406
ApeKI GCWGC 4 cut(s) 346, 941, 954, 1339
ApoI RAATTY 3 cut(s) 397, 562, 686
Asp700I GAANNNNTTC 2 cut(s) 919, 1030
Asp718I GGTACC 2 cut(s) 872, 1106
AspLEI GCGC 2 cut(s) 331, 609
AspS9I GGNCC 1 cut(s) 997
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 6 cut(s) 229, 245, 464, 740, 1058, 1189
AvaII GGWCC 1 cut(s) 997
BamHI GGATCC 1 cut(s) 787
BanI GGYRCC 2 cut(s) 872, 1106
BanII GRGCYC 1 cut(s) 339
BarI GAAGNNNNNNTAC 2 cut(s) 1066, 1098
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 4 cut(s) 333, 928, 941, 1326
BccI CCATC 7 cut(s) 176, 483, 746, 863, 880, 1135, 1259
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BciT130I CCWGG 3 cut(s) 1004, 1217, 1256
BclI TGATCA 1 cut(s) 1153
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 3 cut(s) 61, 160, 648
BfaI CTAG 4 cut(s) 368, 1019, 1130, 1169
BisI GCNGC 5 cut(s) 347, 942, 955, 1340, 1406
BlsI GCNGC 5 cut(s) 348, 943, 956, 1341, 1407
Bme1390I CCNGG 4 cut(s) 14, 1004, 1217, 1256
Bme18I GGWCC 1 cut(s) 997
BmgBI CACGTC 1 cut(s) 612
BmgT120I GGNCC 1 cut(s) 997
BmiI GGNNCC 3 cut(s) 789, 874, 1108
BmrFI CCNGG 4 cut(s) 14, 1004, 1217, 1256
BmrI ACTGGG 1 cut(s) 851
BmsI GCATC 1 cut(s) 610
BmuI ACTGGG 1 cut(s) 851
BoxI GACNNNNGTC 1 cut(s) 1295
BplI GAGNNNNNCTC 2 cut(s) 1166, 1198
BpmI CTGGAG 1 cut(s) 353
BpuEI CTTGAG 3 cut(s) 43, 323, 1376
BpuMI CCSGG 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 669
BsaXI ACNNNNNCTCC 6 cut(s) 179, 209, 622, 647, 652, 677
Bse1I ACTGG 2 cut(s) 857, 1335
Bse3DI GCAATG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 323
BseBI CCWGG 3 cut(s) 1004, 1217, 1256
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 1 cut(s) 1251
BseJI GATNNNNATC 1 cut(s) 323
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 4 cut(s) 289, 857, 1026, 1137
BseNI ACTGG 2 cut(s) 857, 1335
BseRI GAGGAG 2 cut(s) 655, 686
BseXI GCAGC 4 cut(s) 333, 928, 941, 1326
BseYI CCCAGC 1 cut(s) 229
BsgI GTGCAG 1 cut(s) 927
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 1 cut(s) 1408
BshNI GGYRCC 2 cut(s) 872, 1106
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 2 cut(s) 14, 1023
BslFI GGGAC 2 cut(s) 166, 979
BsmAI GTCTC 3 cut(s) 61, 160, 648
BsmBI CGTCTC 2 cut(s) 61, 160
BsmFI GGGAC 2 cut(s) 166, 979
BsmI GAATGC 1 cut(s) 704
BsnI GGCC 1 cut(s) 1408
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 6 cut(s) 244, 300, 787, 1060, 1153, 1179
BspACI CCGC 5 cut(s) 184, 912, 1322, 1405, 1412
BspANI GGCC 1 cut(s) 1408
BspCNI CTCAG 4 cut(s) 288, 856, 1027, 1138
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 3 cut(s) 789, 874, 1108
BspPI GGATC 4 cut(s) 295, 782, 795, 1055
BspT107I GGYRCC 2 cut(s) 872, 1106
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 2 cut(s) 857, 1335
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 6 cut(s) 244, 300, 787, 1060, 1153, 1179
BssNI GRCGYC 1 cut(s) 153
Bst2UI CCWGG 3 cut(s) 1004, 1217, 1256
Bst4CI ACNGT 4 cut(s) 292, 896, 938, 1294
Bst6I CTCTTC 3 cut(s) 163, 429, 1167
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 2 cut(s) 365, 1410
BstDEI CTNAG 5 cut(s) 275, 297, 843, 1035, 1146
BstF5I GGATG 1 cut(s) 1251
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 2 cut(s) 331, 609
BstKTI GATC 6 cut(s) 247, 303, 790, 1063, 1156, 1182
BstMAI GTCTC 3 cut(s) 61, 160, 648
BstMBI GATC 6 cut(s) 244, 300, 787, 1060, 1153, 1179
BstMWI GCNNNNNNNGC 1 cut(s) 343
BstNI CCWGG 3 cut(s) 1004, 1217, 1256
BstNSI RCATGY 1 cut(s) 950
BstPAI GACNNNNGTC 1 cut(s) 1295
BstSCI CCNGG 4 cut(s) 12, 1002, 1215, 1254
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 4 cut(s) 333, 928, 941, 1326
BstX2I RGATCY 2 cut(s) 300, 787
BstYI RGATCY 2 cut(s) 300, 787
BsuRI GGCC 1 cut(s) 1408
BtrI CACGTC 1 cut(s) 612
BtsCI GGATG 1 cut(s) 1251
BtsIMutI CAGTG 3 cut(s) 943, 1328, 1389
Cac8I GCNNGC 2 cut(s) 365, 1410
CfoI GCGC 2 cut(s) 331, 609
Cfr13I GGNCC 1 cut(s) 997
CseI GACGC 1 cut(s) 142
Csp6I GTAC 7 cut(s) 439, 451, 485, 873, 1080, 1107, 1191
CviAII CATG 3 cut(s) 947, 1343, 1370
CviQI GTAC 7 cut(s) 439, 451, 485, 873, 1080, 1107, 1191
DdeI CTNAG 5 cut(s) 275, 297, 843, 1035, 1146
DpnI GATC 6 cut(s) 246, 302, 789, 1062, 1155, 1181
DpnII GATC 6 cut(s) 244, 300, 787, 1060, 1153, 1179
Eam1104I CTCTTC 3 cut(s) 163, 429, 1167
EarI CTCTTC 3 cut(s) 163, 429, 1167
Ecl136II GAGCTC 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 997
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 643, 945
EcoICRI GAGCTC 1 cut(s) 337
EcoRII CCWGG 3 cut(s) 1002, 1215, 1254
EcoT38I GRGCYC 1 cut(s) 339
Esp3I CGTCTC 2 cut(s) 61, 160
FaeI CATG 3 cut(s) 950, 1346, 1373
FaqI GGGAC 2 cut(s) 166, 979
FatI CATG 3 cut(s) 946, 1342, 1369
FauI CCCGC 1 cut(s) 191
FbaI TGATCA 1 cut(s) 1153
FblI GTMKAC 2 cut(s) 108, 530
Fnu4HI GCNGC 5 cut(s) 347, 942, 955, 1340, 1406
FokI GGATG 1 cut(s) 1238
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 5 cut(s) 347, 942, 955, 1340, 1406
FspBI CTAG 4 cut(s) 368, 1019, 1130, 1169
FspI TGCGCA 1 cut(s) 608
GlaI GCGC 2 cut(s) 330, 608
GluI GCNGC 5 cut(s) 347, 942, 955, 1340, 1406
GsaI CCCAGC 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 1 cut(s) 1408
HapII CCGG 2 cut(s) 14, 1023
HgaI GACGC 1 cut(s) 142
HhaI GCGC 2 cut(s) 331, 609
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 3 cut(s) 950, 1346, 1373
Hin6I GCGC 2 cut(s) 329, 607
HinP1I GCGC 2 cut(s) 329, 607
HincII GTYRAC 4 cut(s) 97, 288, 531, 615
HindII GTYRAC 4 cut(s) 97, 288, 531, 615
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 3 cut(s) 6, 174, 1186
HpaI GTTAAC 1 cut(s) 97
HpaII CCGG 2 cut(s) 14, 1023
HphI GGTGA 6 cut(s) 229, 245, 464, 740, 1058, 1189
Hpy166II GTNNAC 7 cut(s) 97, 109, 288, 531, 615, 985, 1076
Hpy188I TCNGA 5 cut(s) 173, 385, 670, 925, 1036
Hpy188III TCNNGA 7 cut(s) 264, 304, 340, 845, 1064, 1183, 1355
Hpy8I GTNNAC 7 cut(s) 97, 109, 288, 531, 615, 985, 1076
Hpy99I CGWCG 3 cut(s) 86, 89, 131
HpyAV CCTTC 3 cut(s) 214, 414, 956
HpyCH4III ACNGT 4 cut(s) 292, 896, 938, 1294
HpyCH4IV ACGT 3 cut(s) 93, 611, 1078
HpyCH4V TGCA 8 cut(s) 242, 551, 580, 623, 702, 863, 944, 1342
HpyF10VI GCNNNNNNNGC 1 cut(s) 343
HpyF3I CTNAG 5 cut(s) 275, 297, 843, 1035, 1146
HpySE526I ACGT 3 cut(s) 93, 611, 1078
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 3 cut(s) 950, 1346, 1373
HspAI GCGC 2 cut(s) 329, 607
KpnI GGTACC 2 cut(s) 876, 1110
Ksp22I TGATCA 1 cut(s) 1153
KspAI GTTAAC 1 cut(s) 97
Kzo9I GATC 6 cut(s) 244, 300, 787, 1060, 1153, 1179
LmnI GCTCC 2 cut(s) 334, 668
Lsp1109I GCAGC 4 cut(s) 333, 928, 941, 1326
LweI GCATC 1 cut(s) 610
MaeI CTAG 4 cut(s) 368, 1019, 1130, 1169
MaeII ACGT 3 cut(s) 93, 611, 1078
MaeIII GTNAC 2 cut(s) 292, 1054
MalI GATC 6 cut(s) 246, 302, 789, 1062, 1155, 1181
MboI GATC 6 cut(s) 244, 300, 787, 1060, 1153, 1179
MboII GAAGA 9 cut(s) 150, 446, 748, 821, 912, 971, 1150, 1184, 1343
MflI RGATCY 2 cut(s) 300, 787
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 9 cut(s) 307, 397, 562, 599, 678, 686, 903, 1116, 1276
MlyI GAGTC 1 cut(s) 1180
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 8 cut(s) 205, 664, 676, 766, 841, 1030, 1137, 1168
MroXI GAANNNNTTC 2 cut(s) 919, 1030
MseI TTAA 7 cut(s) 96, 321, 405, 677, 803, 1089, 1244
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 954
MspI CCGG 2 cut(s) 14, 1023
MspR9I CCNGG 4 cut(s) 14, 1004, 1217, 1256
Mva1269I GAATGC 1 cut(s) 704
MvaI CCWGG 3 cut(s) 1004, 1217, 1256
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 1 cut(s) 343
NciI CCSGG 1 cut(s) 14
NdeII GATC 6 cut(s) 244, 300, 787, 1060, 1153, 1179
NlaIII CATG 3 cut(s) 950, 1346, 1373
NlaIV GGNNCC 3 cut(s) 789, 874, 1108
NmuCI GTSAC 1 cut(s) 292
NsbI TGCGCA 1 cut(s) 608
NspI RCATGY 1 cut(s) 950
PciI ACATGT 1 cut(s) 946
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 90, 108
PctI GAATGC 1 cut(s) 704
PdmI GAANNNNTTC 2 cut(s) 919, 1030
PfeI GAWTC 2 cut(s) 6, 174
PflFI GACNNNGTC 2 cut(s) 209, 989
PfoI TCCNGGA 2 cut(s) 1215, 1254
PkrI GCNGC 5 cut(s) 348, 943, 956, 1341, 1407
PleI GAGTC 1 cut(s) 1180
PpsI GAGTC 1 cut(s) 1180
PscI ACATGT 1 cut(s) 946
PshAI GACNNNNGTC 1 cut(s) 1295
PsiI TTATAA 1 cut(s) 908
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 93
Psp6I CCWGG 3 cut(s) 1002, 1215, 1254
PspFI CCCAGC 1 cut(s) 229
PspGI CCWGG 3 cut(s) 1002, 1215, 1254
PspN4I GGNNCC 3 cut(s) 789, 874, 1108
PspPI GGNCC 1 cut(s) 997
PsuI RGATCY 2 cut(s) 300, 787
PsyI GACNNNGTC 2 cut(s) 209, 989
PvuII CAGCTG 1 cut(s) 954
RsaI GTAC 7 cut(s) 440, 452, 486, 874, 1081, 1108, 1192
RsaNI GTAC 7 cut(s) 439, 451, 485, 873, 1080, 1107, 1191
RseI CAYNNNNRTG 1 cut(s) 252
SacI GAGCTC 1 cut(s) 339
SalI GTCGAC 1 cut(s) 529
SaqAI TTAA 7 cut(s) 96, 321, 405, 677, 803, 1089, 1244
SatI GCNGC 5 cut(s) 347, 942, 955, 1340, 1406
Sau3AI GATC 6 cut(s) 244, 300, 787, 1060, 1153, 1179
Sau96I GGNCC 1 cut(s) 997
SchI GAGTC 1 cut(s) 1180
ScrFI CCNGG 4 cut(s) 14, 1004, 1217, 1256
SduI GDGCHC 1 cut(s) 339
SfaNI GCATC 1 cut(s) 610
SinI GGWCC 1 cut(s) 997
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 3 cut(s) 58, 338, 1355
SmoI CTYRAG 3 cut(s) 58, 338, 1355
Sse9I AATT 9 cut(s) 307, 397, 562, 599, 678, 686, 903, 1116, 1276
SsiI CCGC 5 cut(s) 184, 912, 1322, 1405, 1412
SspMI CTAG 4 cut(s) 368, 1019, 1130, 1169
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 4 cut(s) 12, 1002, 1215, 1254
TaaI ACNGT 4 cut(s) 292, 896, 938, 1294
TaiI ACGT 3 cut(s) 96, 614, 1081
TaqI TCGA 5 cut(s) 81, 161, 280, 530, 1184
TasI AATT 9 cut(s) 307, 397, 562, 599, 678, 686, 903, 1116, 1276
TatI WGTACW 1 cut(s) 438
TauI GCSGC 1 cut(s) 1408
TfiI GAWTC 2 cut(s) 6, 174
Tru1I TTAA 7 cut(s) 96, 321, 405, 677, 803, 1089, 1244
Tru9I TTAA 7 cut(s) 96, 321, 405, 677, 803, 1089, 1244
TscAI CASTG 3 cut(s) 943, 1335, 1389
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 4 cut(s) 346, 941, 954, 1339
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 5 cut(s) 44, 612, 761, 1260, 1368
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 3 cut(s) 943, 1335, 1389
Tth111I GACNNNGTC 2 cut(s) 209, 989
VpaK11BI GGWCC 1 cut(s) 997
XapI RAATTY 3 cut(s) 397, 562, 686
XceI RCATGY 1 cut(s) 950
XcmI CCANNNNNNNNNTGG 2 cut(s) 146, 883
XmiI GTMKAC 2 cut(s) 108, 530
XmnI GAANNNNTTC 2 cut(s) 919, 1030
XspI CTAG 4 cut(s) 368, 1019, 1130, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.