Rw1G009900

phosphotransferase activity, carboxyl group as acceptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002381
Physical Location & Seq
Reverse (-)
21961581 .. 21970646
9066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009900.1

Sequence Viewer

Length: 1314 bp
ATGTCGACGGAGATGAGGAAAGATAAGCAAGTAGATGCAAACAGCAGCGAGGAAGAGTTGGACAAAGGCGTCTCTTTGACTTCCACCAGGTTCTCTTCTCGCAACACTTCCTCCAAATACGACTTTGTCAAGGTGAAGGTGTGGCTGGGAGATAATGCGGATCACTACTACGTGCTTTCCAGGTTTCTGCTCAGCCGAATGTTAACTGTCACCAAGATCCCAAATTATGTAGCTATTAAAATCGCTCTGGAGCTCAAGAAGCTGCTAATAGACAACAGCCTTCTAGATGTTTCCCAGTCTGATTTGGAAGCCAATCTCTTTAAGCTTATGGAACGAAGGGGTTATGGCGAAGAGTACATAAGTCGTTACAAGATGATGACCAGATTTCATCATCAAAGAGTGCCGTTAGTTATTCTTGTCTGTGGAACTGCTTGTGTCGGGAAGTCAACCATTGCTACCCAACTTGCACAAAGGCTAAATTTGCCCAATGTCTTGCAGACAGATATGGTGTACGAGTTACTGCGCACATCAACAGATGCACCATTGACATCTACTCCTGTTTGGGCGCGAGACTTCAGCTCCTCTGAGGAGTTAATTACTGAATTTTGCAGGGAATGCAGGATTGTTCGTAAAGGATTGGCTGGTGATTTGAAGAAAGCAATGAAAGATGGAAAGCCAATAATAATAGAGGGAATACATTTGGATCCAAGCATTTATTTAATGGATGATGAAAATAAAACACGAGCTGATGTGCAAGAGAAAACTCCGGAGGCAAACCATCTTGCAGCGAATGATTCAAAAACACAGATGGGAAATGATTCTCTAATATATACCGAGCATGTCAACTCTGAAGAAGGGACACTAAATAACAAAGTCAACAATGTCTCAGATTACCTGGAAGCTATTAACCTTGCTGGAAGTTTGTCTGATAAAAAAGGTGAAAATCTTAGTGGTGAAGAGACAAACAAAAATTCTCTTGTTAAGAAAGAGAAGTCCGGTCCGGAACCAATAATTATACCTATAGTTCTGAAGATGGCTGAATTTGATCATAAGGCTTTGCTAGAGGAGGAGATCTCAACACGAACATTTAGTGATAAATGTCTAGTCCAGGATAAAGATAAACTAATAAGCAACTTAAAGATCATCCAGGACTATCTTTGCTCATTCAATTCTCAGGGATTGACAGTAGTCAATATATCAGCAACAACGTTCCCACAAACATTGGATTGGCTGCATGGATATCTTCTTGAGTGTATTGAGCATGGTATTTCATCTGTGTCCAACGAAAATAGTAGGCGGCCTGCGGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_18

Protein Analysis

437

Amino Acids

49.27

Weight (kDa)

5.73

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_18 PF13238 138 - 237 4e-07 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
Acc16I TGCGCA 1 cut(s) 524
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 568
AccIII TCCGGA 2 cut(s) 766, 1000
AciI CCGC 3 cut(s) 158, 1297, 1304
AclI AACGTT 1 cut(s) 1208
AclWI GGATC 4 cut(s) 168, 211, 698, 711
AcsI RAATTY 4 cut(s) 478, 602, 970, 1040
AcuI CTGAAG 3 cut(s) 559, 870, 1049
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 2 cut(s) 356, 512
AgsI TTSAA 3 cut(s) 652, 798, 1168
AjnI CCWGG 5 cut(s) 86, 179, 894, 1107, 1146
AluBI AGCT 7 cut(s) 233, 253, 262, 325, 579, 746, 902
AluI AGCT 7 cut(s) 233, 253, 262, 325, 579, 746, 902
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 4 cut(s) 76, 564, 889, 953
AlwI GGATC 4 cut(s) 168, 211, 698, 711
Aor13HI TCCGGA 2 cut(s) 766, 1000
AoxI GGCC 1 cut(s) 1298
ApeKI GCWGC 4 cut(s) 45, 262, 785, 1231
ApoI RAATTY 4 cut(s) 478, 602, 970, 1040
Asp700I GAANNNNTTC 1 cut(s) 817
AspLEI GCGC 2 cut(s) 525, 568
AspS9I GGNCC 1 cut(s) 998
AsuHPI GGTGA 5 cut(s) 145, 202, 656, 950, 965
AvaII GGWCC 1 cut(s) 998
BamHI GGATCC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 255
BauI CACGAG 1 cut(s) 741
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 4 cut(s) 57, 249, 797, 1218
BccI CCATC 4 cut(s) 662, 786, 802, 1027
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 5 cut(s) 88, 181, 896, 1109, 1148
BclI TGATCA 1 cut(s) 1045
BcoDI GTCTC 4 cut(s) 76, 564, 889, 953
BfaI CTAG 3 cut(s) 284, 1061, 1103
BfmI CTRYAG 1 cut(s) 1020
BglII AGATCT 1 cut(s) 1071
BisI GCNGC 5 cut(s) 46, 263, 786, 1232, 1298
BlpI GCTNAGC 1 cut(s) 191
BlsI GCNGC 5 cut(s) 47, 264, 787, 1233, 1299
Bme1390I CCNGG 5 cut(s) 88, 181, 896, 1109, 1148
Bme18I GGWCC 1 cut(s) 998
BmgT120I GGNCC 1 cut(s) 998
BmiI GGNNCC 2 cut(s) 705, 1005
BmrFI CCNGG 5 cut(s) 88, 181, 896, 1109, 1148
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 2 cut(s) 25, 526
BmuI ACTGGG 1 cut(s) 289
BoxI GACNNNNGTC 1 cut(s) 1187
BplI GAGNNNNNCTC 2 cut(s) 1058, 1090
BpmI CTGGAG 1 cut(s) 269
Bpu1102I GCTNAGC 1 cut(s) 191
BpuEI CTTGAG 2 cut(s) 239, 1268
BsaAI YACGTR 1 cut(s) 172
BsaHI GRCGYC 1 cut(s) 69
BsaWI WCCGGW 3 cut(s) 766, 995, 1000
BsaXI ACNNNNNCTCC 6 cut(s) 95, 125, 538, 563, 568, 593
Bse1I ACTGG 1 cut(s) 295
Bse3DI GCAATG 2 cut(s) 450, 666
BseAI TCCGGA 2 cut(s) 766, 1000
BseBI CCWGG 5 cut(s) 88, 181, 896, 1109, 1148
BseGI GGATG 2 cut(s) 730, 1143
BseMI GCAATG 2 cut(s) 450, 666
BseMII CTCAG 4 cut(s) 205, 576, 900, 1187
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 4 cut(s) 571, 602, 1079, 1082
BseXI GCAGC 4 cut(s) 57, 249, 797, 1218
BseYI CCCAGC 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 568
BshFI GGCC 1 cut(s) 1300
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 3 cut(s) 767, 996, 1001
BslFI GGGAC 1 cut(s) 871
BsmAI GTCTC 4 cut(s) 76, 564, 889, 953
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 871
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 1 cut(s) 1300
Bsp1286I GDGCHC 1 cut(s) 255
Bsp13I TCCGGA 2 cut(s) 766, 1000
Bsp143I GATC 6 cut(s) 160, 216, 703, 1045, 1071, 1140
Bsp1720I GCTNAGC 1 cut(s) 191
BspACI CCGC 3 cut(s) 158, 1297, 1304
BspANI GGCC 1 cut(s) 1300
BspCNI CTCAG 4 cut(s) 204, 577, 899, 1186
BspEI TCCGGA 2 cut(s) 766, 1000
BspFNI CGCG 1 cut(s) 568
BspLI GGNNCC 2 cut(s) 705, 1005
BspPI GGATC 4 cut(s) 168, 211, 698, 711
BsrDI GCAATG 2 cut(s) 450, 666
BsrI ACTGG 1 cut(s) 295
BssMI GATC 6 cut(s) 160, 216, 703, 1045, 1071, 1140
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 1 cut(s) 741
Bst2BI CACGAG 1 cut(s) 741
Bst2UI CCWGG 5 cut(s) 88, 181, 896, 1109, 1148
Bst4CI ACNGT 2 cut(s) 208, 1186
Bst6I CTCTTC 4 cut(s) 48, 100, 345, 951
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 615
BstBAI YACGTR 1 cut(s) 172
BstC8I GCNNGC 1 cut(s) 1302
BstDEI CTNAG 5 cut(s) 191, 585, 886, 947, 1173
BstF5I GGATG 2 cut(s) 730, 1143
BstFNI CGCG 1 cut(s) 568
BstHHI GCGC 2 cut(s) 525, 568
BstKTI GATC 6 cut(s) 163, 219, 706, 1048, 1074, 1143
BstMAI GTCTC 4 cut(s) 76, 564, 889, 953
BstMBI GATC 6 cut(s) 160, 216, 703, 1045, 1071, 1140
BstMWI GCNNNNNNNGC 3 cut(s) 259, 481, 615
BstNI CCWGG 5 cut(s) 88, 181, 896, 1109, 1148
BstNSI RCATGY 1 cut(s) 842
BstPAI GACNNNNGTC 1 cut(s) 1187
BstSCI CCNGG 5 cut(s) 86, 179, 894, 1107, 1146
BstSFI CTRYAG 1 cut(s) 1020
BstUI CGCG 1 cut(s) 568
BstV1I GCAGC 4 cut(s) 57, 249, 797, 1218
BstX2I RGATCY 3 cut(s) 216, 703, 1071
BstYI RGATCY 3 cut(s) 216, 703, 1071
BsuRI GGCC 1 cut(s) 1300
BtsCI GGATG 2 cut(s) 730, 1143
Cac8I GCNNGC 1 cut(s) 1302
CfoI GCGC 2 cut(s) 525, 568
Cfr13I GGNCC 1 cut(s) 998
CpoI CGGWCCG 1 cut(s) 998
CseI GACGC 1 cut(s) 58
CsiI ACCWGGT 1 cut(s) 86
Csp6I GTAC 2 cut(s) 355, 511
CspCI CAANNNNNGTGG 2 cut(s) 1203, 1238
CspI CGGWCCG 1 cut(s) 998
CviAII CATG 3 cut(s) 839, 1235, 1262
CviQI GTAC 2 cut(s) 355, 511
DdeI CTNAG 5 cut(s) 191, 585, 886, 947, 1173
DpnI GATC 6 cut(s) 162, 218, 705, 1047, 1073, 1142
DpnII GATC 6 cut(s) 160, 216, 703, 1045, 1071, 1140
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 4 cut(s) 48, 100, 345, 951
EarI CTCTTC 4 cut(s) 48, 100, 345, 951
Ecl136II GAGCTC 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 255
Eco32I GATATC 1 cut(s) 1241
Eco47I GGWCC 1 cut(s) 998
Eco53kI GAGCTC 1 cut(s) 253
Eco57I CTGAAG 3 cut(s) 559, 870, 1049
EcoICRI GAGCTC 1 cut(s) 253
EcoRII CCWGG 5 cut(s) 86, 179, 894, 1107, 1146
EcoRV GATATC 1 cut(s) 1241
EcoT38I GRGCYC 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 3 cut(s) 842, 1238, 1265
FaqI GGGAC 1 cut(s) 871
FatI CATG 3 cut(s) 838, 1234, 1261
FbaI TGATCA 1 cut(s) 1045
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 46, 263, 786, 1232, 1298
FokI GGATG 2 cut(s) 737, 1130
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 5 cut(s) 46, 263, 786, 1232, 1298
FspBI CTAG 3 cut(s) 284, 1061, 1103
FspI TGCGCA 1 cut(s) 524
GlaI GCGC 2 cut(s) 524, 567
GluI GCNGC 5 cut(s) 46, 263, 786, 1232, 1298
GsaI CCCAGC 1 cut(s) 149
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 1 cut(s) 1300
HapII CCGG 3 cut(s) 767, 996, 1001
HgaI GACGC 1 cut(s) 58
HhaI GCGC 2 cut(s) 525, 568
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 3 cut(s) 842, 1238, 1265
Hin6I GCGC 2 cut(s) 523, 566
HinP1I GCGC 2 cut(s) 523, 566
HincII GTYRAC 5 cut(s) 6, 204, 447, 844, 877
HindII GTYRAC 5 cut(s) 6, 204, 447, 844, 877
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 2 cut(s) 794, 818
HpaI GTTAAC 1 cut(s) 204
HpaII CCGG 3 cut(s) 767, 996, 1001
HphI GGTGA 5 cut(s) 145, 202, 656, 950, 965
Hpy166II GTNNAC 6 cut(s) 6, 204, 447, 511, 844, 877
Hpy188I TCNGA 6 cut(s) 301, 586, 850, 889, 928, 1029
Hpy188III TCNNGA 7 cut(s) 248, 256, 284, 439, 767, 1001, 1247
Hpy8I GTNNAC 6 cut(s) 6, 204, 447, 511, 844, 877
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 130, 290, 330, 848
HpyCH4III ACNGT 2 cut(s) 208, 1186
HpyCH4IV ACGT 2 cut(s) 171, 1208
HpyCH4V TGCA 9 cut(s) 38, 467, 496, 539, 609, 618, 754, 785, 1234
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 481, 615
HpyF3I CTNAG 5 cut(s) 191, 585, 886, 947, 1173
HpySE526I ACGT 2 cut(s) 171, 1208
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 3 cut(s) 842, 1238, 1265
HspAI GCGC 2 cut(s) 523, 566
Kpn2I TCCGGA 2 cut(s) 766, 1000
Ksp22I TGATCA 1 cut(s) 1045
KspAI GTTAAC 1 cut(s) 204
Kzo9I GATC 6 cut(s) 160, 216, 703, 1045, 1071, 1140
LmnI GCTCC 2 cut(s) 250, 584
Lsp1109I GCAGC 4 cut(s) 57, 249, 797, 1218
LweI GCATC 2 cut(s) 25, 526
MabI ACCWGGT 1 cut(s) 86
MaeI CTAG 3 cut(s) 284, 1061, 1103
MaeII ACGT 2 cut(s) 171, 1208
MaeIII GTNAC 3 cut(s) 208, 365, 516
MalI GATC 6 cut(s) 162, 218, 705, 1047, 1073, 1142
MboI GATC 6 cut(s) 160, 216, 703, 1045, 1071, 1140
MboII GAAGA 8 cut(s) 65, 87, 362, 664, 863, 968, 1042, 1235
MflI RGATCY 3 cut(s) 216, 703, 1071
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 8 cut(s) 223, 478, 594, 602, 970, 1011, 1040, 1168
MmeI TCCRAC 2 cut(s) 39, 1305
MnlI CCTC 9 cut(s) 9, 43, 121, 580, 592, 682, 763, 1057, 1060
MroI TCCGGA 2 cut(s) 766, 1000
MroXI GAANNNNTTC 1 cut(s) 817
MseI TTAA 8 cut(s) 203, 237, 321, 593, 719, 906, 981, 1136
MspI CCGG 3 cut(s) 767, 996, 1001
MspR9I CCNGG 5 cut(s) 88, 181, 896, 1109, 1148
Mva1269I GAATGC 1 cut(s) 620
MvaI CCWGG 5 cut(s) 88, 181, 896, 1109, 1148
MvnI CGCG 1 cut(s) 568
MwoI GCNNNNNNNGC 3 cut(s) 259, 481, 615
NdeII GATC 6 cut(s) 160, 216, 703, 1045, 1071, 1140
NlaIII CATG 3 cut(s) 842, 1238, 1265
NlaIV GGNNCC 2 cut(s) 705, 1005
NmuCI GTSAC 1 cut(s) 208
NsbI TGCGCA 1 cut(s) 524
NspI RCATGY 1 cut(s) 842
PctI GAATGC 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 817
PfeI GAWTC 2 cut(s) 794, 818
PflFI GACNNNGTC 1 cut(s) 125
PfoI TCCNGGA 2 cut(s) 1107, 1146
PkrI GCNGC 5 cut(s) 47, 264, 787, 1233, 1299
Ppu21I YACGTR 1 cut(s) 172
PshAI GACNNNNGTC 1 cut(s) 1187
Psp124BI GAGCTC 1 cut(s) 255
Psp1406I AACGTT 1 cut(s) 1208
Psp6I CCWGG 5 cut(s) 86, 179, 894, 1107, 1146
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 5 cut(s) 86, 179, 894, 1107, 1146
PspN4I GGNNCC 2 cut(s) 705, 1005
PspPI GGNCC 1 cut(s) 998
PsuI RGATCY 3 cut(s) 216, 703, 1071
PsyI GACNNNGTC 1 cut(s) 125
RsaI GTAC 2 cut(s) 356, 512
RsaNI GTAC 2 cut(s) 355, 511
Rsr2I CGGWCCG 1 cut(s) 998
RsrII CGGWCCG 1 cut(s) 998
SacI GAGCTC 1 cut(s) 255
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 8 cut(s) 203, 237, 321, 593, 719, 906, 981, 1136
SatI GCNGC 5 cut(s) 46, 263, 786, 1232, 1298
Sau3AI GATC 6 cut(s) 160, 216, 703, 1045, 1071, 1140
Sau96I GGNCC 1 cut(s) 998
ScrFI CCNGG 5 cut(s) 88, 181, 896, 1109, 1148
SduI GDGCHC 1 cut(s) 255
SexAI ACCWGGT 1 cut(s) 86
SfaNI GCATC 2 cut(s) 25, 526
SfcI CTRYAG 1 cut(s) 1020
SinI GGWCC 1 cut(s) 998
SmlI CTYRAG 2 cut(s) 254, 1247
SmoI CTYRAG 2 cut(s) 254, 1247
Sse9I AATT 8 cut(s) 223, 478, 594, 602, 970, 1011, 1040, 1168
SsiI CCGC 3 cut(s) 158, 1297, 1304
SspMI CTAG 3 cut(s) 284, 1061, 1103
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 5 cut(s) 86, 179, 894, 1107, 1146
TaaI ACNGT 2 cut(s) 208, 1186
TaiI ACGT 2 cut(s) 174, 1211
TaqI TCGA 1 cut(s) 5
TasI AATT 8 cut(s) 223, 478, 594, 602, 970, 1011, 1040, 1168
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 1300
TfiI GAWTC 2 cut(s) 794, 818
Tru1I TTAA 8 cut(s) 203, 237, 321, 593, 719, 906, 981, 1136
Tru9I TTAA 8 cut(s) 203, 237, 321, 593, 719, 906, 981, 1136
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 4 cut(s) 45, 262, 785, 1231
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 4 cut(s) 377, 677, 744, 1260
TspGWI ACGGA 1 cut(s) 23
Tth111I GACNNNGTC 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 998
XapI RAATTY 4 cut(s) 478, 602, 970, 1040
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 817
XspI CTAG 3 cut(s) 284, 1061, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.