MD07G1252600.v1.1

phosphotransferase activity, carboxyl group as acceptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002381
Physical Location & Seq
Forward (+)
32034767 .. 32037220
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1252600.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGGGTCCTAGAACCATAACTGCTTCCTCCAAATACGACTTTGTCAAGGTGAAGGTGTGGTTGGGTGATCATGCCGAGCACTACTACGTGTTTTCGAGGTTTTTGCTCAGCCAAATGTTAACCGTCACCAAGATCCCAAATCATGCAGCTATCAGAATCGCTCTTGAGCTCAAGAAGCTGCTTGTGGACAACAGCCTTCTAGATGTGTCGCAGTCCGATTTGGAAGCTAATATGTTCAAGCTTATGGAGCAGAGGGGCTATGGGGAAGAGTACGCAAATCGTTACAAGATGATGACGAGATTTCACCATCAAAGAGTACCATTGATCATTCTTTTATGCGGAACTGCCTGCGTTGGAAAGTCGACGATTGCAACACAAATTTCACAGAGGTTGAATTTGCCTAATGTCATGCAGACAGATGTGGTGTACGAATTGCTGCGCACATCAACGGATGCACCATTGGCATCTACTCCTGTATGGGCGCGAGACTTCAGCTCCGCGGAGGAGTTAATTACCGAGTTTTGTAGGGAATGCAGGATTGTACGTAAAGGATTGGCGGGTGATTTGAAGAAAGCAGTGAAAGATGGGAAGCCAATAATAATAGAAGGAATGCATATCGATCCAAACATCTATCTGATGGCGGATGAAAATAAAACACCAGACGAATCCAGAACACACTTGGAAAATGTGCTTGTCAGCTCAGAAGAAGGGACACCAACAGCTAACAACGCGAACAACGTAGATTACCTGGAAGCTATTGGCCTAGCAGGAAGTGTCATTGAGATTAGTAACGAAGGTGAAAGCGTGAAAGGTTCGGAGGAGGTTAAAAGTACTTCTGTTTCGAAAGAGAAGTGTGGTCCTGGACCGATTATTATACCTCTAGTTCTGAAGATGGCTGAATTCGATCACAATGCATTACTAGAGGAATGGTTCTCAACTTTTGCATTCAATGACAAACTAGTCCAGGACAAAGATAAGCTAGCAAGCAACTTAAAGATCATTCAGGACTATCTTTGTTCATTCAACTCCAAGGGATTGAAAGTACTCAACCTGTCAACAAACACATTCAACCCGACGTTGGACTGGCTGCACGGATATCTTCTCGAGGTACATCTTCTGAGTTATGATAAAACATGTTCGAATTCAAGCAATCCTGCGTGCTTCTTAAATCTGCAAAACTTGGATGTCGATGTCCAACTCAATATGTTATTCCTTGTCTACGTACACTGCGAACTGGTAGTAATCTTACTCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_18

Protein Analysis

419

Amino Acids

46.93

Weight (kDa)

5.53

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_18 PF13238 110 - 205 3.7e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 959
Acc16I TGCGCA 1 cut(s) 440
AccI GTMKAC 2 cut(s) 362, 1218
AccII CGCG 3 cut(s) 484, 500, 731
AciI CCGC 5 cut(s) 339, 498, 500, 557, 641
AclWI GGATC 2 cut(s) 127, 614
AcsI RAATTY 4 cut(s) 378, 394, 899, 1141
AcuI CTGAAG 2 cut(s) 475, 908
AfaI GTAC 8 cut(s) 272, 318, 428, 543, 832, 1044, 1110, 1224
AfiI CCNNNNNNNGG 1 cut(s) 1078
AflIII ACRYGT 2 cut(s) 87, 1133
AgsI TTSAA 8 cut(s) 238, 394, 568, 949, 1024, 1039, 1069, 1146
AhlI ACTAGT 1 cut(s) 958
AjnI CCWGG 3 cut(s) 747, 859, 963
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
Alw21I GWGCWC 2 cut(s) 81, 171
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 2 cut(s) 127, 614
Ama87I CYCGRG 1 cut(s) 1103
AoxI GGCC 1 cut(s) 760
ApeKI GCWGC 4 cut(s) 146, 178, 436, 1087
ApoI RAATTY 4 cut(s) 378, 394, 899, 1141
AspLEI GCGC 2 cut(s) 441, 484
AspS9I GGNCC 3 cut(s) 5, 857, 863
AsuHPI GGTGA 6 cut(s) 61, 77, 118, 296, 572, 809
AsuII TTCGAA 2 cut(s) 842, 1139
AsuNHI GCTAGC 1 cut(s) 979
AvaI CYCGRG 1 cut(s) 1103
AvaII GGWCC 3 cut(s) 5, 857, 863
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 81, 171
BbvI GCAGC 4 cut(s) 158, 165, 423, 1074
BccI CCATC 4 cut(s) 315, 578, 631, 886
BcgI CGANNNNNNTGC 4 cut(s) 85, 119, 893, 927
BciT130I CCWGG 3 cut(s) 749, 861, 965
BclI TGATCA 2 cut(s) 67, 324
BcoDI GTCTC 1 cut(s) 480
BcuI ACTAGT 1 cut(s) 958
BfaI CTAG 8 cut(s) 9, 200, 764, 881, 920, 959, 980, 1255
BisI GCNGC 4 cut(s) 147, 179, 437, 1088
BlpI GCTNAGC 1 cut(s) 107
BlsI GCNGC 4 cut(s) 148, 180, 438, 1089
BmcAI AGTACT 2 cut(s) 832, 1044
Bme1390I CCNGG 3 cut(s) 749, 861, 965
Bme18I GGWCC 3 cut(s) 5, 857, 863
BmeT110I CYCGRG 1 cut(s) 1103
BmgT120I GGNCC 3 cut(s) 5, 857, 863
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 749, 861, 965
BmsI GCATC 2 cut(s) 442, 473
BmtI GCTAGC 1 cut(s) 983
Bpu1102I GCTNAGC 1 cut(s) 107
Bpu14I TTCGAA 2 cut(s) 842, 1139
BpuEI CTTGAG 2 cut(s) 155, 185
Bsa29I ATCGAT 1 cut(s) 618
BsaAI YACGTR 3 cut(s) 88, 545, 1222
BsaJI CCNNGG 2 cut(s) 498, 1029
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 1078
Bse1I ACTGG 2 cut(s) 1088, 1239
BseBI CCWGG 3 cut(s) 749, 861, 965
BseCI ATCGAT 1 cut(s) 618
BseDI CCNNGG 2 cut(s) 498, 1029
BseGI GGATG 3 cut(s) 457, 649, 1189
BseLI CCNNNNNNNGG 1 cut(s) 1078
BseMII CTCAG 3 cut(s) 121, 714, 1109
BseNI ACTGG 2 cut(s) 1088, 1239
BseRI GAGGAG 2 cut(s) 518, 833
BseXI GCAGC 4 cut(s) 158, 165, 423, 1074
BsgI GTGCAG 1 cut(s) 1073
Bsh1236I CGCG 3 cut(s) 484, 500, 731
BshFI GGCC 1 cut(s) 762
BshVI ATCGAT 1 cut(s) 618
BsiHKAI GWGCWC 2 cut(s) 81, 171
BsiHKCI CYCGRG 1 cut(s) 1103
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 1 cut(s) 1078
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 3 cut(s) 536, 615, 944
BsnI GGCC 1 cut(s) 762
BsoBI CYCGRG 1 cut(s) 1103
Bsp119I TTCGAA 2 cut(s) 842, 1139
Bsp1286I GDGCHC 2 cut(s) 81, 171
Bsp143I GATC 6 cut(s) 67, 132, 324, 619, 904, 996
Bsp1720I GCTNAGC 1 cut(s) 107
BspACI CCGC 5 cut(s) 339, 498, 500, 557, 641
BspANI GGCC 1 cut(s) 762
BspCNI CTCAG 3 cut(s) 120, 713, 1110
BspDI ATCGAT 1 cut(s) 618
BspFNI CGCG 3 cut(s) 484, 500, 731
BspLI GGNNCC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 983
BspPI GGATC 2 cut(s) 127, 614
BspT104I TTCGAA 2 cut(s) 842, 1139
BsrI ACTGG 2 cut(s) 1088, 1239
BssECI CCNNGG 2 cut(s) 498, 1029
BssMI GATC 6 cut(s) 67, 132, 324, 619, 904, 996
BssT1I CCWWGG 1 cut(s) 1029
Bst2UI CCWGG 3 cut(s) 749, 861, 965
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 1 cut(s) 261
BstBAI YACGTR 3 cut(s) 88, 545, 1222
BstBI TTCGAA 2 cut(s) 842, 1139
BstC8I GCNNGC 4 cut(s) 349, 981, 985, 1159
BstDEI CTNAG 3 cut(s) 107, 700, 1118
BstDSI CCRYGG 1 cut(s) 498
BstF5I GGATG 3 cut(s) 457, 649, 1189
BstFNI CGCG 3 cut(s) 484, 500, 731
BstHHI GCGC 2 cut(s) 441, 484
BstKTI GATC 6 cut(s) 70, 135, 327, 622, 907, 999
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 6 cut(s) 67, 132, 324, 619, 904, 996
BstMWI GCNNNNNNNGC 4 cut(s) 175, 247, 461, 728
BstNI CCWGG 3 cut(s) 749, 861, 965
BstNSI RCATGY 1 cut(s) 1137
BstSCI CCNGG 3 cut(s) 747, 859, 963
BstSNI TACGTA 2 cut(s) 545, 1222
BstUI CGCG 3 cut(s) 484, 500, 731
BstV1I GCAGC 4 cut(s) 158, 165, 423, 1074
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
Bsu15I ATCGAT 1 cut(s) 618
BsuRI GGCC 1 cut(s) 762
BsuTUI ATCGAT 1 cut(s) 618
BtgI CCRYGG 1 cut(s) 498
BtsCI GGATG 3 cut(s) 457, 649, 1189
BtsI GCAGTG 2 cut(s) 582, 1225
BtsIMutI CAGTG 2 cut(s) 582, 1225
Cac8I GCNNGC 4 cut(s) 349, 981, 985, 1159
CfoI GCGC 2 cut(s) 441, 484
Cfr13I GGNCC 3 cut(s) 5, 857, 863
Cfr42I CCGCGG 1 cut(s) 501
ClaI ATCGAT 1 cut(s) 618
Csp6I GTAC 8 cut(s) 271, 317, 427, 542, 831, 1043, 1109, 1223
CviAII CATG 4 cut(s) 71, 143, 409, 1134
CviQI GTAC 8 cut(s) 271, 317, 427, 542, 831, 1043, 1109, 1223
DdeI CTNAG 3 cut(s) 107, 700, 1118
DpnI GATC 6 cut(s) 69, 134, 326, 621, 906, 998
DpnII GATC 6 cut(s) 67, 132, 324, 619, 904, 996
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
EciI GGCGGA 1 cut(s) 656
Ecl136II GAGCTC 1 cut(s) 169
Eco105I TACGTA 2 cut(s) 545, 1222
Eco130I CCWWGG 1 cut(s) 1029
Eco24I GRGCYC 1 cut(s) 171
Eco32I GATATC 1 cut(s) 1097
Eco47I GGWCC 3 cut(s) 5, 857, 863
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 475, 908
Eco88I CYCGRG 1 cut(s) 1103
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 2 cut(s) 899, 1141
EcoRII CCWGG 3 cut(s) 747, 859, 963
EcoRV GATATC 1 cut(s) 1097
EcoT14I CCWWGG 1 cut(s) 1029
EcoT22I ATGCAT 2 cut(s) 615, 916
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 1029
FaeI CATG 4 cut(s) 74, 146, 412, 1137
FaqI GGGAC 1 cut(s) 724
FatI CATG 4 cut(s) 70, 142, 408, 1133
FauI CCCGC 1 cut(s) 550
FbaI TGATCA 2 cut(s) 67, 324
FblI GTMKAC 2 cut(s) 362, 1218
Fnu4HI GCNGC 4 cut(s) 147, 179, 437, 1088
FokI GGATG 3 cut(s) 464, 656, 1196
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 4 cut(s) 147, 179, 437, 1088
FspBI CTAG 8 cut(s) 9, 200, 764, 881, 920, 959, 980, 1255
FspI TGCGCA 1 cut(s) 440
GlaI GCGC 2 cut(s) 440, 483
GluI GCNGC 4 cut(s) 147, 179, 437, 1088
HaeIII GGCC 1 cut(s) 762
HhaI GCGC 2 cut(s) 441, 484
Hin1II CATG 4 cut(s) 74, 146, 412, 1137
Hin6I GCGC 2 cut(s) 439, 482
HinP1I GCGC 2 cut(s) 439, 482
HincII GTYRAC 3 cut(s) 120, 363, 1056
HindII GTYRAC 3 cut(s) 120, 363, 1056
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 2 cut(s) 156, 665
HpaI GTTAAC 1 cut(s) 120
HphI GGTGA 6 cut(s) 61, 77, 118, 296, 572, 809
Hpy166II GTNNAC 7 cut(s) 120, 187, 363, 427, 1056, 1219, 1225
Hpy188I TCNGA 7 cut(s) 155, 217, 636, 703, 817, 888, 1119
Hpy188III TCNNGA 6 cut(s) 164, 172, 200, 669, 1004, 1103
Hpy8I GTNNAC 7 cut(s) 120, 187, 363, 427, 1056, 1219, 1225
Hpy99I CGWCG 2 cut(s) 367, 1078
HpyAV CCTTC 5 cut(s) 46, 206, 599, 701, 788
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 5 cut(s) 87, 544, 738, 1076, 1221
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 247, 461, 728
HpyF3I CTNAG 3 cut(s) 107, 700, 1118
HpySE526I ACGT 5 cut(s) 87, 544, 738, 1076, 1221
Hsp92II CATG 4 cut(s) 74, 146, 412, 1137
HspAI GCGC 2 cut(s) 439, 482
Ksp22I TGATCA 2 cut(s) 67, 324
KspAI GTTAAC 1 cut(s) 120
KspI CCGCGG 1 cut(s) 501
Kzo9I GATC 6 cut(s) 67, 132, 324, 619, 904, 996
LmnI GCTCC 2 cut(s) 247, 500
Lsp1109I GCAGC 4 cut(s) 158, 165, 423, 1074
LweI GCATC 2 cut(s) 442, 473
MaeI CTAG 8 cut(s) 9, 200, 764, 881, 920, 959, 980, 1255
MaeII ACGT 5 cut(s) 87, 544, 738, 1076, 1221
MaeIII GTNAC 3 cut(s) 124, 281, 788
MalI GATC 6 cut(s) 69, 134, 326, 621, 906, 998
MboI GATC 6 cut(s) 67, 132, 324, 619, 904, 996
MboII GAAGA 6 cut(s) 278, 580, 716, 901, 1091, 1106
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 2 cut(s) 81, 171
MluCI AATT 6 cut(s) 378, 394, 431, 510, 899, 1141
MmeI TCCRAC 3 cut(s) 334, 1059, 1219
Mph1103I ATGCAT 2 cut(s) 615, 916
MseI TTAA 5 cut(s) 119, 509, 825, 992, 1166
MspA1I CMGCKG 1 cut(s) 500
MspR9I CCNGG 3 cut(s) 749, 861, 965
Mva1269I GAATGC 3 cut(s) 536, 615, 944
MvaI CCWGG 3 cut(s) 749, 861, 965
MvnI CGCG 3 cut(s) 484, 500, 731
MwoI GCNNNNNNNGC 4 cut(s) 175, 247, 461, 728
NdeII GATC 6 cut(s) 67, 132, 324, 619, 904, 996
NheI GCTAGC 1 cut(s) 979
NlaIII CATG 4 cut(s) 74, 146, 412, 1137
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 100
NmuCI GTSAC 1 cut(s) 124
NsbI TGCGCA 1 cut(s) 440
NsiI ATGCAT 2 cut(s) 615, 916
NspI RCATGY 1 cut(s) 1137
NspV TTCGAA 2 cut(s) 842, 1139
PaeR7I CTCGAG 1 cut(s) 1103
PciI ACATGT 1 cut(s) 1133
PcsI WCGNNNNNNNCGW 2 cut(s) 735, 1227
PctI GAATGC 3 cut(s) 536, 615, 944
PfeI GAWTC 2 cut(s) 156, 665
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 2 cut(s) 859, 963
PkrI GCNGC 4 cut(s) 148, 180, 438, 1089
Ppu21I YACGTR 3 cut(s) 88, 545, 1222
PpuMI RGGWCCY 1 cut(s) 5
PscI ACATGT 1 cut(s) 1133
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 3 cut(s) 747, 859, 963
PspGI CCWGG 3 cut(s) 747, 859, 963
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 5, 857, 863
PspPPI RGGWCCY 1 cut(s) 5
PsuI RGATCY 1 cut(s) 132
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 8 cut(s) 272, 318, 428, 543, 832, 1044, 1110, 1224
RsaNI GTAC 8 cut(s) 271, 317, 427, 542, 831, 1043, 1109, 1223
SacI GAGCTC 1 cut(s) 171
SacII CCGCGG 1 cut(s) 501
SalI GTCGAC 1 cut(s) 361
SaqAI TTAA 5 cut(s) 119, 509, 825, 992, 1166
SatI GCNGC 4 cut(s) 147, 179, 437, 1088
Sau3AI GATC 6 cut(s) 67, 132, 324, 619, 904, 996
Sau96I GGNCC 3 cut(s) 5, 857, 863
ScaI AGTACT 2 cut(s) 832, 1044
ScrFI CCNGG 3 cut(s) 749, 861, 965
SduI GDGCHC 2 cut(s) 81, 171
SfaNI GCATC 2 cut(s) 442, 473
Sfr274I CTCGAG 1 cut(s) 1103
Sfr303I CCGCGG 1 cut(s) 501
SfuI TTCGAA 2 cut(s) 842, 1139
SgrBI CCGCGG 1 cut(s) 501
SinI GGWCC 3 cut(s) 5, 857, 863
SlaI CTCGAG 1 cut(s) 1103
SmlI CTYRAG 3 cut(s) 164, 170, 1103
SmoI CTYRAG 3 cut(s) 164, 170, 1103
SnaBI TACGTA 2 cut(s) 545, 1222
SpeI ACTAGT 1 cut(s) 958
Sse9I AATT 6 cut(s) 378, 394, 431, 510, 899, 1141
SsiI CCGC 5 cut(s) 339, 498, 500, 557, 641
SspMI CTAG 8 cut(s) 9, 200, 764, 881, 920, 959, 980, 1255
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 747, 859, 963
StyI CCWWGG 1 cut(s) 1029
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 5 cut(s) 90, 547, 741, 1079, 1224
TaqI TCGA 8 cut(s) 95, 362, 618, 842, 903, 1104, 1139, 1188
TaqII GACCGA 1 cut(s) 880
TasI AATT 6 cut(s) 378, 394, 431, 510, 899, 1141
TatI WGTACW 2 cut(s) 830, 1042
TfiI GAWTC 2 cut(s) 156, 665
Tru1I TTAA 5 cut(s) 119, 509, 825, 992, 1166
Tru9I TTAA 5 cut(s) 119, 509, 825, 992, 1166
TscAI CASTG 2 cut(s) 582, 1232
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 4 cut(s) 146, 178, 436, 1087
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 2 cut(s) 660, 1008
TspGWI ACGGA 2 cut(s) 464, 1107
TspRI CASTG 2 cut(s) 582, 1232
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 3 cut(s) 5, 857, 863
XapI RAATTY 4 cut(s) 378, 394, 899, 1141
XbaI TCTAGA 1 cut(s) 199
XceI RCATGY 1 cut(s) 1137
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XhoI CTCGAG 1 cut(s) 1103
XmiI GTMKAC 2 cut(s) 362, 1218
XspI CTAG 8 cut(s) 9, 200, 764, 881, 920, 959, 980, 1255
ZrmI AGTACT 2 cut(s) 832, 1044
Zsp2I ATGCAT 2 cut(s) 615, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.