MD01G1182200.v1.1

phosphotransferase activity, carboxyl group as acceptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002381
Physical Location & Seq
Forward (+)
28097024 .. 28102157
5134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1182200.v1.1.491

Sequence Viewer

Length: 1392 bp
ATGAACAACAACAAAGGCAAAGCAGTCGCCGTCTCAAGCCCCAACGTCAACGACATCGACGACGACGACGACGGCAACGGTAACATCAGCGAAGACGAATTGGACGACGACAACAACAGAAGCAGTGGCTTCTCTTCCACTTCCATCAGATTCTCGTCCCGCAACACTTCCTCCAAATACGACTTCGTCAAGGTGAAGGTCTGGCTGGGTGATAATGCAGACCACTACTACGTTCTGTCCAGATTTTTGCTCAGTCGAATGTTGACTGTCACCAAGATCCCGAATTATGTAGCGATTAAAATAGCGCTGGAGCTCAAGAAGTTGCTGATTGACAACAGCCTGCTAGATGTTTCGCAGTCCGATTTGGAAGTGAATTTGTTTAAGCTTATGGAACGAAGGGGTTATGGGGAAGAGTACATAAATCGGTACAAGATGATGACCAGATTTCACCATCAACGAGTGCCTTTAGTTATTCTTGTATGTGGAACTGCCTGTGTTGGAAAGTCGACCATTGCTACCCAACTCGCACAAAGACTAAATTTGCCTAATGTCTTGCAGACGGATATGGTATATGAATTGCTGCGCACGGCAACAGATGCACCGTTGACATCTACTCCTGTATGGGCACGAGATTTCAGCTCCTCTGAGGAATTAATTACTGAATTTTGTAGGGAATGCAGGATTGTCCGTAAAGGATTGGGTGGTGATATGAAGAAAGCTCTGAAAGATGGAAAGCCAATAATAATAGAGGGAATACATTTGGATCCAAGCATTTATTTAATGGAAGATGATAATAAAACACAAGAACAAGAGAAAACTCAGGAGGCAGACCCCGTTGCAATAGATGGCTCCAAAACACAGACGGAAAACAATTCTCCAAGTGGAAGTTCATCTGAAGTTTGCAATGGTACTGCACATGTCAGCTGCAAAGAAGGGACACCAACTAACAAGGTGGATGAAGTCTCGAATCACCTGGAAGCTATTGGTCTAGCCGGAAGTGTTTCTGAGGAAAAAGGTGAAAGTGTTACTGATCCAGAGTTGAGTAAAAGTACTTATGTTAAGAAAGAGAAGTCTGGTACTAAACCAATTATTATACCTATAGTTTTGAATATGGCTGAATTTGATCATAAGGCATTACTAGAAGAGGAGATCTCGACTCCTACATTTAGTGAAAAATCTATAGTCCAGGATAAAGATAAGCTAATACGCAACTTAAAGACCATCCAGGACTATCTTTGTTCATTCAATTCGCAGGGATTGAAAGTCGTCAATATATCAGCAACCACATTTCCGCAAACACTGGATTGGCTGCACGGTTATCTTCTTGAGTGTATAGAGCATGGTATTTCATCAGTGTCCAATGAAAATAGTAGGCAGCCTGCGGAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_18

Protein Analysis

464

Amino Acids

51.5

Weight (kDa)

5.11

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_18 PF13238 158 - 260 1.5e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 584
AccI GTMKAC 1 cut(s) 506
AciI CCGC 3 cut(s) 160, 1292, 1382
AclWI GGATC 4 cut(s) 271, 758, 771, 1025
AcsI RAATTY 5 cut(s) 373, 538, 662, 1118, 1386
AcuI CTGAAG 1 cut(s) 915
AfaI GTAC 5 cut(s) 416, 428, 910, 1051, 1078
AfeI AGCGCT 1 cut(s) 306
AflIII ACRYGT 1 cut(s) 916
AgsI TTSAA 3 cut(s) 1108, 1246, 1261
AjnI CCWGG 3 cut(s) 972, 1185, 1224
AjuI GAANNNNNNNTTGG 4 cut(s) 167, 199, 871, 903
AluBI AGCT 7 cut(s) 313, 385, 639, 719, 924, 980, 1201
AluI AGCT 7 cut(s) 313, 385, 639, 719, 924, 980, 1201
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 37, 967
AlwI GGATC 4 cut(s) 271, 758, 771, 1025
Aor51HI AGCGCT 1 cut(s) 306
ApeKI GCWGC 4 cut(s) 580, 924, 1309, 1375
ApoI RAATTY 5 cut(s) 373, 538, 662, 1118, 1386
AseI ATTAAT 1 cut(s) 653
Asp700I GAANNNNTTC 1 cut(s) 1000
AspLEI GCGC 2 cut(s) 307, 585
AsuHPI GGTGA 7 cut(s) 205, 221, 262, 440, 716, 962, 1028
BaeGI GKGCMC 1 cut(s) 628
BamHI GGATCC 1 cut(s) 763
BanII GRGCYC 1 cut(s) 315
BauI CACGAG 1 cut(s) 627
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 4 cut(s) 567, 911, 1296, 1387
BccI CCATC 5 cut(s) 152, 459, 722, 839, 1229
BceAI ACGGC 3 cut(s) 14, 88, 603
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 3 cut(s) 974, 1187, 1226
BclI TGATCA 1 cut(s) 1123
BcoDI GTCTC 2 cut(s) 37, 967
BfaI CTAG 3 cut(s) 344, 989, 1139
BfmI CTRYAG 2 cut(s) 1098, 1179
BfoI RGCGCY 1 cut(s) 308
BglII AGATCT 1 cut(s) 1149
BisI GCNGC 4 cut(s) 581, 925, 1310, 1376
BlsI GCNGC 4 cut(s) 582, 926, 1311, 1377
BmcAI AGTACT 1 cut(s) 1051
Bme1390I CCNGG 3 cut(s) 974, 1187, 1226
BmiI GGNNCC 2 cut(s) 765, 850
BmrFI CCNGG 3 cut(s) 974, 1187, 1226
BmsI GCATC 1 cut(s) 586
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 1136, 1168
BpmI CTGGAG 1 cut(s) 329
BpuEI CTTGAG 3 cut(s) 19, 299, 1346
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 598, 628
Bse1I ACTGG 1 cut(s) 1305
Bse3DI GCAATG 2 cut(s) 510, 910
BseBI CCWGG 3 cut(s) 974, 1187, 1226
BseGI GGATG 2 cut(s) 961, 1221
BseMI GCAATG 2 cut(s) 510, 910
BseMII CTCAG 4 cut(s) 265, 636, 833, 996
BseNI ACTGG 1 cut(s) 1305
BseRI GAGGAG 2 cut(s) 631, 1160
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 4 cut(s) 567, 911, 1296, 1387
BseYI CCCAGC 1 cut(s) 205
BsgI GTGCAG 2 cut(s) 897, 1295
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 993
BslFI GGGAC 2 cut(s) 142, 949
BsmAI GTCTC 2 cut(s) 37, 967
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 2 cut(s) 142, 949
BsmI GAATGC 1 cut(s) 680
Bsp1286I GDGCHC 2 cut(s) 315, 628
Bsp143I GATC 5 cut(s) 276, 763, 1030, 1123, 1149
BspACI CCGC 3 cut(s) 160, 1292, 1382
BspCNI CTCAG 4 cut(s) 264, 637, 832, 997
BspLI GGNNCC 2 cut(s) 765, 850
BspPI GGATC 4 cut(s) 271, 758, 771, 1025
BsrDI GCAATG 2 cut(s) 510, 910
BsrI ACTGG 1 cut(s) 1305
BssMI GATC 5 cut(s) 276, 763, 1030, 1123, 1149
BssSI CACGAG 1 cut(s) 627
Bst2BI CACGAG 1 cut(s) 627
Bst2UI CCWGG 3 cut(s) 974, 1187, 1226
Bst4CI ACNGT 4 cut(s) 80, 268, 603, 1316
Bst6I CTCTTC 3 cut(s) 139, 405, 1137
BstAPI GCANNNNNTGC 1 cut(s) 596
BstC8I GCNNGC 2 cut(s) 341, 1380
BstDEI CTNAG 4 cut(s) 251, 645, 819, 1005
BstF5I GGATG 2 cut(s) 961, 1221
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 2 cut(s) 307, 585
BstKTI GATC 5 cut(s) 279, 766, 1033, 1126, 1152
BstMAI GTCTC 2 cut(s) 37, 967
BstMBI GATC 5 cut(s) 276, 763, 1030, 1123, 1149
BstMWI GCNNNNNNNGC 1 cut(s) 596
BstNI CCWGG 3 cut(s) 974, 1187, 1226
BstNSI RCATGY 1 cut(s) 920
BstSCI CCNGG 3 cut(s) 972, 1185, 1224
BstSFI CTRYAG 2 cut(s) 1098, 1179
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 4 cut(s) 567, 911, 1296, 1387
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 3 cut(s) 276, 763, 1149
BstYI RGATCY 3 cut(s) 276, 763, 1149
BtsCI GGATG 2 cut(s) 961, 1221
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 3 cut(s) 130, 1298, 1359
Cac8I GCNNGC 2 cut(s) 341, 1380
CfoI GCGC 2 cut(s) 307, 585
Csp6I GTAC 5 cut(s) 415, 427, 909, 1050, 1077
CviAII CATG 2 cut(s) 917, 1340
CviQI GTAC 5 cut(s) 415, 427, 909, 1050, 1077
DdeI CTNAG 4 cut(s) 251, 645, 819, 1005
DpnI GATC 5 cut(s) 278, 765, 1032, 1125, 1151
DpnII GATC 5 cut(s) 276, 763, 1030, 1123, 1149
Eam1104I CTCTTC 3 cut(s) 139, 405, 1137
EarI CTCTTC 3 cut(s) 139, 405, 1137
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 315
Eco47III AGCGCT 1 cut(s) 306
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 915
EcoICRI GAGCTC 1 cut(s) 313
EcoRII CCWGG 3 cut(s) 972, 1185, 1224
EcoT38I GRGCYC 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 2 cut(s) 920, 1343
FaqI GGGAC 2 cut(s) 142, 949
FatI CATG 2 cut(s) 916, 1339
FauI CCCGC 1 cut(s) 167
FbaI TGATCA 1 cut(s) 1123
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 4 cut(s) 581, 925, 1310, 1376
FokI GGATG 2 cut(s) 968, 1208
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 4 cut(s) 581, 925, 1310, 1376
FspBI CTAG 3 cut(s) 344, 989, 1139
FspI TGCGCA 1 cut(s) 584
GlaI GCGC 2 cut(s) 306, 584
GluI GCNGC 4 cut(s) 581, 925, 1310, 1376
GsaI CCCAGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 308
HapII CCGG 1 cut(s) 993
HhaI GCGC 2 cut(s) 307, 585
Hin1II CATG 2 cut(s) 920, 1343
Hin6I GCGC 2 cut(s) 305, 583
HinP1I GCGC 2 cut(s) 305, 583
HincII GTYRAC 4 cut(s) 49, 264, 507, 606
HindII GTYRAC 4 cut(s) 49, 264, 507, 606
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 150, 967, 1156
HpaII CCGG 1 cut(s) 993
HphI GGTGA 7 cut(s) 205, 221, 262, 440, 716, 962, 1028
Hpy166II GTNNAC 4 cut(s) 49, 264, 507, 606
Hpy188I TCNGA 6 cut(s) 149, 361, 646, 723, 895, 1006
Hpy188III TCNNGA 8 cut(s) 240, 280, 316, 821, 964, 1034, 1153, 1325
Hpy8I GTNNAC 4 cut(s) 49, 264, 507, 606
Hpy99I CGWCG 6 cut(s) 62, 65, 68, 71, 74, 110
HpyAV CCTTC 3 cut(s) 190, 390, 926
HpyCH4III ACNGT 4 cut(s) 80, 268, 603, 1316
HpyCH4IV ACGT 2 cut(s) 45, 231
HpyCH4V TGCA 9 cut(s) 218, 556, 599, 678, 839, 903, 914, 927, 1312
HpyF10VI GCNNNNNNNGC 1 cut(s) 596
HpyF3I CTNAG 4 cut(s) 251, 645, 819, 1005
HpySE526I ACGT 2 cut(s) 45, 231
Hsp92II CATG 2 cut(s) 920, 1343
HspAI GCGC 2 cut(s) 305, 583
Ksp22I TGATCA 1 cut(s) 1123
Kzo9I GATC 5 cut(s) 276, 763, 1030, 1123, 1149
LmnI GCTCC 3 cut(s) 310, 644, 854
Lsp1109I GCAGC 4 cut(s) 567, 911, 1296, 1387
LweI GCATC 1 cut(s) 586
MaeI CTAG 3 cut(s) 344, 989, 1139
MaeII ACGT 2 cut(s) 45, 231
MaeIII GTNAC 3 cut(s) 80, 268, 1024
MalI GATC 5 cut(s) 278, 765, 1032, 1125, 1151
MboI GATC 5 cut(s) 276, 763, 1030, 1123, 1149
MboII GAAGA 7 cut(s) 104, 126, 422, 724, 797, 1154, 1313
MflI RGATCY 3 cut(s) 276, 763, 1149
MhlI GDGCHC 2 cut(s) 315, 628
MlyI GAGTC 1 cut(s) 1150
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 7 cut(s) 181, 640, 652, 742, 817, 1000, 1138
MroXI GAANNNNTTC 1 cut(s) 1000
MseI TTAA 6 cut(s) 297, 381, 653, 779, 1059, 1214
MspA1I CMGCKG 1 cut(s) 924
MspI CCGG 1 cut(s) 993
MspR9I CCNGG 3 cut(s) 974, 1187, 1226
Mva1269I GAATGC 1 cut(s) 680
MvaI CCWGG 3 cut(s) 974, 1187, 1226
MwoI GCNNNNNNNGC 1 cut(s) 596
NdeII GATC 5 cut(s) 276, 763, 1030, 1123, 1149
NlaIII CATG 2 cut(s) 920, 1343
NlaIV GGNNCC 2 cut(s) 765, 850
NmuCI GTSAC 1 cut(s) 268
NsbI TGCGCA 1 cut(s) 584
NspI RCATGY 1 cut(s) 920
PciI ACATGT 1 cut(s) 916
PcsI WCGNNNNNNNCGW 4 cut(s) 57, 63, 66, 102
PctI GAATGC 1 cut(s) 680
PdmI GAANNNNTTC 1 cut(s) 1000
PfeI GAWTC 2 cut(s) 150, 967
PflFI GACNNNGTC 1 cut(s) 185
PfoI TCCNGGA 2 cut(s) 1185, 1224
PkrI GCNGC 4 cut(s) 582, 926, 1311, 1377
PleI GAGTC 1 cut(s) 1150
PpsI GAGTC 1 cut(s) 1150
PscI ACATGT 1 cut(s) 916
PshBI ATTAAT 1 cut(s) 653
Psp124BI GAGCTC 1 cut(s) 315
Psp6I CCWGG 3 cut(s) 972, 1185, 1224
PspFI CCCAGC 1 cut(s) 205
PspGI CCWGG 3 cut(s) 972, 1185, 1224
PspN4I GGNNCC 2 cut(s) 765, 850
PsuI RGATCY 3 cut(s) 276, 763, 1149
PsyI GACNNNGTC 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 924
RsaI GTAC 5 cut(s) 416, 428, 910, 1051, 1078
RsaNI GTAC 5 cut(s) 415, 427, 909, 1050, 1077
SacI GAGCTC 1 cut(s) 315
SalI GTCGAC 1 cut(s) 505
SaqAI TTAA 6 cut(s) 297, 381, 653, 779, 1059, 1214
SatI GCNGC 4 cut(s) 581, 925, 1310, 1376
Sau3AI GATC 5 cut(s) 276, 763, 1030, 1123, 1149
ScaI AGTACT 1 cut(s) 1051
SchI GAGTC 1 cut(s) 1150
ScrFI CCNGG 3 cut(s) 974, 1187, 1226
SduI GDGCHC 2 cut(s) 315, 628
SfaNI GCATC 1 cut(s) 586
SfcI CTRYAG 2 cut(s) 1098, 1179
SmlI CTYRAG 3 cut(s) 34, 314, 1325
SmoI CTYRAG 3 cut(s) 34, 314, 1325
SsiI CCGC 3 cut(s) 160, 1292, 1382
SspMI CTAG 3 cut(s) 344, 989, 1139
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 3 cut(s) 972, 1185, 1224
TaaI ACNGT 4 cut(s) 80, 268, 603, 1316
TaiI ACGT 2 cut(s) 48, 234
TaqI TCGA 5 cut(s) 57, 256, 506, 965, 1154
TatI WGTACW 2 cut(s) 414, 1049
TfiI GAWTC 2 cut(s) 150, 967
Tru1I TTAA 6 cut(s) 297, 381, 653, 779, 1059, 1214
Tru9I TTAA 6 cut(s) 297, 381, 653, 779, 1059, 1214
TscAI CASTG 3 cut(s) 130, 1305, 1359
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 4 cut(s) 580, 924, 1309, 1375
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 8 cut(s) 17, 588, 725, 879, 972, 1230, 1338, 1377
TspGWI ACGGA 3 cut(s) 575, 677, 878
TspRI CASTG 3 cut(s) 130, 1305, 1359
Tth111I GACNNNGTC 1 cut(s) 185
VspI ATTAAT 1 cut(s) 653
XapI RAATTY 5 cut(s) 373, 538, 662, 1118, 1386
XceI RCATGY 1 cut(s) 920
XmiI GTMKAC 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 1000
XspI CTAG 3 cut(s) 344, 989, 1139
ZrmI AGTACT 1 cut(s) 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.