FvH4_1g12131

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003681
Physical Location & Seq
Reverse (-)
6626786 .. 6628874
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12131.t1

Sequence Viewer

Length: 1530 bp
ATGGAGGAGCAATACTATGATGACCACAGCAGTTCCAATTTCAAAGATATCATCCCCACTGCGATGTCTTCCTTTTTCTCGGCCTATGCCCCCTTTGCTGGTTACATGTTTATGCTCCAATCCATGAACACCAACTTCATACCTTACGAGTTCAAATCATACGTATACTCATACCTAAAATCAAGCCTCAGCTACCTCTTGGACCCTCAAGCCAATCACATAACTCTCTACGTCCAGCAATATGAGGCAGACAGCTGGAGCGAAATTTCGCAGTGCAACCTAGTCTATGATGCTGCTGAGGTCTACCTTGCCACCAAGATCAACCCGAAAACCAAATACTTTCGAATCAGCCAAGCACCGAAACAAAAGGTACCCATGGTTGCTGTTACCACCAACCAAGAAATCACTGACACTTTCGAGGATATCAAAGTGCAGTGGCGCATGATCTTCCCCGAAAGATACAGTAGTGGCAATAAGTGCCGCATTGAGCTAACACTCCACAGGAAATACAAAGACAAAGTTATAAACTCGTACATACCCTATGTATTGGCTCGGGCCGAGGCTATCAAAGATGCAGAAAAGACTCCAAAGCTTTATAGTTACAATAGTAAACATTCGAATTCGTCAAGAACTTCTTATGGTATGGATGACGATTATGACTACATACCTTCTTCGGACAAATGGAGTTGCGTGGATCTTGAGCACCCGGCTACTTTTGAGACAATTGCAATGGAGCCGGAGCTGAAAAAGGAGATCATAGAGGATTTGGACAAGTTCATGAAGAGGAGGGAGTTCTACAAGAGAGTTGGCAAGGCTTGGAAGAGAGGTTACTTGTTGTATGGTCCACCCGGTACTGGAAAGTCCAGCTTGGTTGCGGCCATGGCTAATCATCTCCATTTTAATATTTATGACTTGGAACTTACTAGTATTTCCAGCAATGAAGAGTTGAGGAAAGCCTTGATGACTACTACAAATCAATCTATTGTGGTCTTTGAGGATATTGATTGCAGCAAGGCATCCGTAAACCGGGAATCAACAGAGAACAAGAACTCTATGGTTTCCCAAGCCAAATCCAAGTTTACACTGTCCGGTCTACTGAATGTCATCGATGGTCTGTGGTCAAGCTGCGGTGATGAGAGGATTATTGTGTTCACCACCAACCACAAGGAGCGGCTAGACCCTGCATTGTTGAGGCCTGGTCGAATGGACATGCACATTCACATGTCTTACTGCACCCCTAAAGGGTTCAAGACCTTAGCCTCGAACTACCTTGGCATTCAGGACACTAGCAAACATCAGCTGTGTCAAGAAATTGAAGGGTTGATGGAGAGCACAAACATCACCCCTGCAGAGGTTTGTGAGGAGCTTATGAGGGGTGGGGGTGGTGACGATGATGATGCTGATATTGCTCTTGAAGGACTTGTCAATTGCCTCAAGCGTAAGAGGCTCGAGACCAACAAGGAAGAAGAGACTGGAATAGCAAAGAGGCAGAAAACAGAGAATTCTGCATCTGAAATTTCTGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

510

Amino Acids

58.26

Weight (kDa)

5.62

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 47 - 148 5.7e-15 Domain associated at C-terminal with AAA
AAA PF00004 277 - 409 5.1e-20 ATPase family associated with various cellular activities (AAA)
RNA_helicase PF00910 278 - 361 3.3e-06 RNA helicase
AAA_lid_At3g28540 PF25568 411 - 488 3.8e-21 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
Acc65I GGTACC 1 cut(s) 370
AccB1I GGYRCC 1 cut(s) 370
AccBSI CCGCTC 1 cut(s) 1171
AccI GTMKAC 3 cut(s) 165, 303, 1093
AciI CCGC 4 cut(s) 481, 875, 1128, 1171
AclWI GGATC 1 cut(s) 702
AcoI YGGCCR 1 cut(s) 876
AcsI RAATTY 4 cut(s) 264, 619, 1501, 1515
AfaI GTAC 3 cut(s) 372, 533, 853
AfiI CCNNNNNNNGG 5 cut(s) 98, 854, 1026, 1242, 1351
AflIII ACRYGT 2 cut(s) 105, 1221
AgsI TTSAA 5 cut(s) 43, 154, 1249, 1316, 1415
AhlI ACTAGT 1 cut(s) 923
AjnI CCWGG 1 cut(s) 1195
AluBI AGCT 9 cut(s) 192, 255, 490, 592, 742, 867, 1125, 1300, 1366
AluI AGCT 9 cut(s) 192, 255, 490, 592, 742, 867, 1125, 1300, 1366
Alw21I GWGCWC 2 cut(s) 705, 1334
Alw26I GTCTC 3 cut(s) 713, 1445, 1463
AlwI GGATC 1 cut(s) 702
Ama87I CYCGRG 2 cut(s) 552, 1448
AoxI GGCC 4 cut(s) 81, 555, 876, 1193
ApeKI GCWGC 3 cut(s) 293, 1008, 1125
ApoI RAATTY 4 cut(s) 264, 619, 1501, 1515
Asp718I GGTACC 1 cut(s) 370
AspLEI GCGC 1 cut(s) 441
AspS9I GGNCC 3 cut(s) 202, 555, 842
AsuC2I CCSGG 3 cut(s) 707, 849, 1028
AsuHPI GGTGA 4 cut(s) 1142, 1144, 1333, 1397
AsuII TTCGAA 2 cut(s) 343, 617
AvaI CYCGRG 2 cut(s) 552, 1448
AvaII GGWCC 2 cut(s) 202, 842
BaeI ACNNNNGTAYC 2 cut(s) 451, 484
BanI GGYRCC 1 cut(s) 370
BarI GAAGNNNNNNTAC 2 cut(s) 812, 844
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 705, 1334
BbvCI CCTCAGC 2 cut(s) 188, 297
BbvI GCAGC 3 cut(s) 280, 1020, 1112
BccI CCATC 2 cut(s) 1103, 1318
BcgI CGANNNNNNTGC 2 cut(s) 1379, 1413
BciT130I CCWGG 1 cut(s) 1197
BcnI CCSGG 3 cut(s) 707, 849, 1028
BcoDI GTCTC 3 cut(s) 713, 1445, 1463
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 4 cut(s) 281, 924, 1175, 1287
BfmI CTRYAG 1 cut(s) 1347
BisI GCNGC 6 cut(s) 294, 481, 876, 1009, 1126, 1172
BlsI GCNGC 6 cut(s) 295, 482, 877, 1010, 1127, 1173
Bme1390I CCNGG 4 cut(s) 707, 849, 1028, 1197
Bme18I GGWCC 2 cut(s) 202, 842
BmeT110I CYCGRG 2 cut(s) 552, 1448
BmgT120I GGNCC 3 cut(s) 202, 555, 842
BmiI GGNNCC 3 cut(s) 204, 372, 735
BmrFI CCNGG 4 cut(s) 707, 849, 1028, 1197
BmsI GCATC 5 cut(s) 280, 562, 1025, 1387, 1517
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 1 cut(s) 277
Bpu10I CCTNAGC 3 cut(s) 188, 297, 1255
Bpu14I TTCGAA 2 cut(s) 343, 617
BpuEI CTTGAG 3 cut(s) 192, 719, 1418
BpuMI CCSGG 3 cut(s) 707, 849, 1028
Bsa29I ATCGAT 1 cut(s) 1107
BsaAI YACGTR 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 1445
BsaJI CCNNGG 4 cut(s) 375, 558, 879, 1270
BsaWI WCCGGW 1 cut(s) 1088
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 854, 1026, 1242, 1351
Bse1I ACTGG 2 cut(s) 859, 1477
Bse3DI GCAATG 2 cut(s) 735, 943
BseBI CCWGG 1 cut(s) 1197
BseCI ATCGAT 1 cut(s) 1107
BseDI CCNNGG 4 cut(s) 375, 558, 879, 1270
BseGI GGATG 3 cut(s) 51, 652, 1016
BseLI CCNNNNNNNGG 5 cut(s) 98, 854, 1026, 1242, 1351
BseMI GCAATG 2 cut(s) 735, 943
BseMII CTCAG 2 cut(s) 202, 288
BseNI ACTGG 2 cut(s) 859, 1477
BseRI GAGGAG 3 cut(s) 20, 799, 1376
BseXI GCAGC 3 cut(s) 280, 1020, 1112
BsgI GTGCAG 2 cut(s) 452, 1216
BshFI GGCC 4 cut(s) 83, 557, 878, 1195
BshNI GGYRCC 1 cut(s) 370
BshVI ATCGAT 1 cut(s) 1107
BsiHKAI GWGCWC 2 cut(s) 705, 1334
BsiHKCI CYCGRG 2 cut(s) 552, 1448
BsiSI CCGG 5 cut(s) 707, 737, 849, 1027, 1089
BslI CCNNNNNNNGG 5 cut(s) 98, 854, 1026, 1242, 1351
BsmAI GTCTC 3 cut(s) 713, 1445, 1463
BsmI GAATGC 1 cut(s) 1275
BsnI GGCC 4 cut(s) 83, 557, 878, 1195
Bso31I GGTCTC 1 cut(s) 1445
BsoBI CYCGRG 2 cut(s) 552, 1448
Bsp119I TTCGAA 2 cut(s) 343, 617
Bsp1286I GDGCHC 2 cut(s) 705, 1334
Bsp143I GATC 4 cut(s) 318, 444, 694, 753
Bsp19I CCATGG 2 cut(s) 375, 879
BspACI CCGC 4 cut(s) 481, 875, 1128, 1171
BspANI GGCC 4 cut(s) 83, 557, 878, 1195
BspCNI CTCAG 2 cut(s) 201, 289
BspDI ATCGAT 1 cut(s) 1107
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 3 cut(s) 204, 372, 735
BspMAI CTGCAG 1 cut(s) 1351
BspPI GGATC 1 cut(s) 702
BspT104I TTCGAA 2 cut(s) 343, 617
BspT107I GGYRCC 1 cut(s) 370
BspTNI GGTCTC 1 cut(s) 1445
BsrBI CCGCTC 1 cut(s) 1171
BsrDI GCAATG 2 cut(s) 735, 943
BsrI ACTGG 2 cut(s) 859, 1477
BssECI CCNNGG 4 cut(s) 375, 558, 879, 1270
BssMI GATC 4 cut(s) 318, 444, 694, 753
BssNAI GTATAC 1 cut(s) 166
BssT1I CCWWGG 3 cut(s) 375, 879, 1270
Bst1107I GTATAC 1 cut(s) 166
Bst2UI CCWGG 1 cut(s) 1197
Bst4CI ACNGT 2 cut(s) 464, 1086
Bst6I CTCTTC 4 cut(s) 776, 815, 936, 1461
BstAPI GCANNNNNTGC 1 cut(s) 477
BstBAI YACGTR 1 cut(s) 163
BstBI TTCGAA 2 cut(s) 343, 617
BstDEI CTNAG 3 cut(s) 188, 297, 1255
BstDSI CCRYGG 2 cut(s) 375, 879
BstF5I GGATG 3 cut(s) 51, 652, 1016
BstHHI GCGC 1 cut(s) 441
BstKTI GATC 4 cut(s) 321, 447, 697, 756
BstMAI GTCTC 3 cut(s) 713, 1445, 1463
BstMBI GATC 4 cut(s) 318, 444, 694, 753
BstMWI GCNNNNNNNGC 5 cut(s) 95, 477, 881, 1406, 1444
BstNI CCWGG 1 cut(s) 1197
BstNSI RCATGY 3 cut(s) 109, 1213, 1225
BstSCI CCNGG 4 cut(s) 705, 847, 1026, 1195
BstSFI CTRYAG 1 cut(s) 1347
BstSNI TACGTA 1 cut(s) 163
BstV1I GCAGC 3 cut(s) 280, 1020, 1112
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BstZ17I GTATAC 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 1107
BsuRI GGCC 4 cut(s) 83, 557, 878, 1195
BsuTUI ATCGAT 1 cut(s) 1107
BtgI CCRYGG 2 cut(s) 375, 879
BtgZI GCGATG 1 cut(s) 77
BtsCI GGATG 3 cut(s) 51, 652, 1016
BtsI GCAGTG 3 cut(s) 57, 278, 440
BtsIMutI CAGTG 5 cut(s) 57, 278, 405, 440, 1082
CciI TCATGA 1 cut(s) 777
CfoI GCGC 1 cut(s) 441
Cfr13I GGNCC 3 cut(s) 202, 555, 842
ClaI ATCGAT 1 cut(s) 1107
Csp6I GTAC 3 cut(s) 371, 532, 852
CviAII CATG 8 cut(s) 106, 124, 376, 442, 778, 880, 1210, 1222
CviQI GTAC 3 cut(s) 371, 532, 852
DdeI CTNAG 3 cut(s) 188, 297, 1255
DpnI GATC 4 cut(s) 320, 446, 696, 755
DpnII GATC 4 cut(s) 318, 444, 694, 753
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 4 cut(s) 776, 815, 936, 1461
EarI CTCTTC 4 cut(s) 776, 815, 936, 1461
Eco105I TACGTA 1 cut(s) 163
Eco130I CCWWGG 3 cut(s) 375, 879, 1270
Eco147I AGGCCT 1 cut(s) 1195
Eco31I GGTCTC 1 cut(s) 1445
Eco32I GATATC 2 cut(s) 49, 424
Eco47I GGWCC 2 cut(s) 202, 842
Eco88I CYCGRG 2 cut(s) 552, 1448
EcoRI GAATTC 2 cut(s) 619, 1501
EcoRII CCWGG 1 cut(s) 1195
EcoRV GATATC 2 cut(s) 49, 424
EcoT14I CCWWGG 3 cut(s) 375, 879, 1270
ErhI CCWWGG 3 cut(s) 375, 879, 1270
FaeI CATG 8 cut(s) 109, 127, 379, 445, 781, 883, 1213, 1225
FalI AAGNNNNNCTT 4 cut(s) 619, 651, 851, 883
FatI CATG 8 cut(s) 105, 123, 375, 441, 777, 879, 1209, 1221
FblI GTMKAC 3 cut(s) 165, 303, 1093
Fnu4HI GCNGC 6 cut(s) 294, 481, 876, 1009, 1126, 1172
FokI GGATG 3 cut(s) 38, 659, 1003
Fsp4HI GCNGC 6 cut(s) 294, 481, 876, 1009, 1126, 1172
FspBI CTAG 4 cut(s) 281, 924, 1175, 1287
GlaI GCGC 1 cut(s) 440
GluI GCNGC 6 cut(s) 294, 481, 876, 1009, 1126, 1172
GsuI CTGGAG 1 cut(s) 277
HaeIII GGCC 4 cut(s) 83, 557, 878, 1195
HapII CCGG 5 cut(s) 707, 737, 849, 1027, 1089
HhaI GCGC 1 cut(s) 441
Hin1II CATG 8 cut(s) 109, 127, 379, 445, 781, 883, 1213, 1225
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 345, 583, 1031
HpaII CCGG 5 cut(s) 707, 737, 849, 1027, 1089
HphI GGTGA 4 cut(s) 1142, 1144, 1333, 1397
Hpy166II GTNNAC 8 cut(s) 166, 304, 611, 845, 1024, 1080, 1094, 1152
Hpy188I TCNGA 3 cut(s) 676, 1513, 1522
Hpy188III TCNNGA 8 cut(s) 627, 698, 778, 1249, 1280, 1307, 1412, 1450
Hpy8I GTNNAC 8 cut(s) 166, 304, 611, 845, 1024, 1080, 1094, 1152
HpyAV CCTTC 3 cut(s) 678, 1310, 1409
HpyCH4III ACNGT 2 cut(s) 464, 1086
HpyCH4IV ACGT 2 cut(s) 162, 231
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 477, 881, 1406, 1444
HpyF3I CTNAG 3 cut(s) 188, 297, 1255
HpySE526I ACGT 2 cut(s) 162, 231
Hsp92II CATG 8 cut(s) 109, 127, 379, 445, 781, 883, 1213, 1225
HspAI GCGC 1 cut(s) 439
KpnI GGTACC 1 cut(s) 374
Kzo9I GATC 4 cut(s) 318, 444, 694, 753
LmnI GCTCC 7 cut(s) 7, 120, 258, 733, 739, 1168, 1363
Lsp1109I GCAGC 3 cut(s) 280, 1020, 1112
LweI GCATC 5 cut(s) 280, 562, 1025, 1387, 1517
MaeI CTAG 4 cut(s) 281, 924, 1175, 1287
MaeII ACGT 2 cut(s) 162, 231
MaeIII GTNAC 5 cut(s) 101, 385, 599, 827, 1385
MalI GATC 4 cut(s) 320, 446, 696, 755
MbiI CCGCTC 1 cut(s) 1171
MboI GATC 4 cut(s) 318, 444, 694, 753
MboII GAAGA 8 cut(s) 60, 439, 663, 793, 832, 953, 1475, 1478
MfeI CAATTG 2 cut(s) 723, 1426
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 2 cut(s) 705, 1334
MluCI AATT 9 cut(s) 37, 264, 619, 723, 1311, 1426, 1501, 1515, 1525
MlyI GAGTC 1 cut(s) 577
MseI TTAA 1 cut(s) 900
MslI CAYNNNNRTG 3 cut(s) 62, 110, 1220
MspA1I CMGCKG 2 cut(s) 255, 1300
MspI CCGG 5 cut(s) 707, 737, 849, 1027, 1089
MspR9I CCNGG 4 cut(s) 707, 849, 1028, 1197
MunI CAATTG 2 cut(s) 723, 1426
Mva1269I GAATGC 1 cut(s) 1275
MvaI CCWGG 1 cut(s) 1197
MwoI GCNNNNNNNGC 5 cut(s) 95, 477, 881, 1406, 1444
NciI CCSGG 3 cut(s) 707, 849, 1028
NcoI CCATGG 2 cut(s) 375, 879
NdeII GATC 4 cut(s) 318, 444, 694, 753
NlaIII CATG 8 cut(s) 109, 127, 379, 445, 781, 883, 1213, 1225
NlaIV GGNNCC 3 cut(s) 204, 372, 735
NmeAIII GCCGAG 2 cut(s) 59, 583
NmuCI GTSAC 1 cut(s) 1385
NspI RCATGY 3 cut(s) 109, 1213, 1225
NspV TTCGAA 2 cut(s) 343, 617
PaeR7I CTCGAG 1 cut(s) 1448
PagI TCATGA 1 cut(s) 777
PceI AGGCCT 1 cut(s) 1195
PciI ACATGT 2 cut(s) 105, 1221
PctI GAATGC 1 cut(s) 1275
PfeI GAWTC 2 cut(s) 345, 1031
PkrI GCNGC 6 cut(s) 295, 482, 877, 1010, 1127, 1173
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
Ppu21I YACGTR 1 cut(s) 163
PscI ACATGT 2 cut(s) 105, 1221
PsiI TTATAA 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 1195
PspGI CCWGG 1 cut(s) 1195
PspN4I GGNNCC 3 cut(s) 204, 372, 735
PspPI GGNCC 3 cut(s) 202, 555, 842
PstI CTGCAG 1 cut(s) 1351
PsuI RGATCY 1 cut(s) 694
PvuII CAGCTG 2 cut(s) 255, 1300
RsaI GTAC 3 cut(s) 372, 533, 853
RsaNI GTAC 3 cut(s) 371, 532, 852
RseI CAYNNNNRTG 3 cut(s) 62, 110, 1220
SaqAI TTAA 1 cut(s) 900
SatI GCNGC 6 cut(s) 294, 481, 876, 1009, 1126, 1172
Sau3AI GATC 4 cut(s) 318, 444, 694, 753
Sau96I GGNCC 3 cut(s) 202, 555, 842
SchI GAGTC 1 cut(s) 577
ScrFI CCNGG 4 cut(s) 707, 849, 1028, 1197
SduI GDGCHC 2 cut(s) 705, 1334
SfaNI GCATC 5 cut(s) 280, 562, 1025, 1387, 1517
SfcI CTRYAG 1 cut(s) 1347
Sfr274I CTCGAG 1 cut(s) 1448
SfuI TTCGAA 2 cut(s) 343, 617
SinI GGWCC 2 cut(s) 202, 842
SlaI CTCGAG 1 cut(s) 1448
SmiMI CAYNNNNRTG 3 cut(s) 62, 110, 1220
SmlI CTYRAG 4 cut(s) 207, 698, 1433, 1448
SmoI CTYRAG 4 cut(s) 207, 698, 1433, 1448
SnaBI TACGTA 1 cut(s) 163
SpeI ACTAGT 1 cut(s) 923
Sse9I AATT 9 cut(s) 37, 264, 619, 723, 1311, 1426, 1501, 1515, 1525
SseBI AGGCCT 1 cut(s) 1195
SsiI CCGC 4 cut(s) 481, 875, 1128, 1171
SspI AATATT 1 cut(s) 904
SspMI CTAG 4 cut(s) 281, 924, 1175, 1287
StuI AGGCCT 1 cut(s) 1195
StyD4I CCNGG 4 cut(s) 705, 847, 1026, 1195
StyI CCWWGG 3 cut(s) 375, 879, 1270
TaaI ACNGT 2 cut(s) 464, 1086
TaiI ACGT 2 cut(s) 165, 234
TaqI TCGA 7 cut(s) 343, 417, 617, 1107, 1201, 1262, 1449
TasI AATT 9 cut(s) 37, 264, 619, 723, 1311, 1426, 1501, 1515, 1525
TauI GCSGC 3 cut(s) 483, 878, 1174
TfiI GAWTC 2 cut(s) 345, 1031
Tru1I TTAA 1 cut(s) 900
Tru9I TTAA 1 cut(s) 900
TscAI CASTG 5 cut(s) 64, 278, 412, 440, 1089
TseFI GTSAC 1 cut(s) 1385
TseI GCWGC 3 cut(s) 293, 1008, 1125
Tsp45I GTSAC 1 cut(s) 1385
TspDTI ATGAA 5 cut(s) 127, 140, 766, 794, 954
TspGWI ACGGA 1 cut(s) 1009
TspRI CASTG 5 cut(s) 64, 278, 412, 440, 1089
VpaK11BI GGWCC 2 cut(s) 202, 842
XapI RAATTY 4 cut(s) 264, 619, 1501, 1515
XceI RCATGY 3 cut(s) 109, 1213, 1225
XhoI CTCGAG 1 cut(s) 1448
XmiI GTMKAC 3 cut(s) 165, 303, 1093
XspI CTAG 4 cut(s) 281, 924, 1175, 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.