Rh2DG138000

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003681
Physical Location & Seq
Reverse (-)
11531027 .. 11534041
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG138000.1

Sequence Viewer

Length: 1518 bp
ATGGGAATTAAGATGACTTCTCCAAATTTGCAAGATTTGATGCCCACAGCAATGTCTTCCTTTTTCTCGGCCTATGCCCCCTTCGCTGGTTACATGTTTATGCTCCAATCCTTGAACACCAATTTCATACCTTACCAGGTCAAATCATACATATACAAATACTTCAAATCAAGCATCAACTACCTCCTGGACCCTCAAGCTAATCACCTAACTCTCCTCGTCGACTGTTCTAGCCATCGCAACTACTCCTCCGGAATCTCAATGGCTCAGCGCAACCAACTCTATGATGCCGCTGAGGTCTATCTCGCCACCAAGATCAGTCCGAAAACCCAAGTTTTTCGAATCACCAAAACATCTAAACAAAAGACCCCTAAACTTGCCGTTACCACCACCCAAGAAATCACCGACACTTTCCAAAACATTAAAGTAAAGTGGCACATGATCTTCCGTTCCAATGATGACCATCACTACAGTAGTGACCAGAATGCCCGCATTGAGTTAACATTCCCTAGGAAACACAAAGATAAGGTGTTGGACTCGTACATCCCCTATGTGTTGGCTCAGGCCGAGGCTATTGAAGATGCAGAAAAGACTCTGAAGCTTTATAGTTATGACGTTAAACGTTCCATCCACTACAACCCTACTCGTGGTAGTGATGACGAGGGCTACGGACCTTCTTCGGGCAATTGGAGTTGCGTAGATCTTGAGCACCCAGCTACTTTCGAGACAATAGCCATGGAGCCGGAGCTGAAGAGGATGATCATAGAGGATTTGGACAAGTTCGTGAAGAGGAGGGAGTTCTACAAGAGAGTTGGCAAGGCTTGGAAGAGAGGCTACTTGTTGTTTGGTCCACCCGGTACTGGAAAATCCAGCTTGGTTGCGGCCATGGCTAATCATCTCCATTTTAACATTTATGACTTGGAACTTACCAGTATTTCCAGCAATGATGAGTTGAGGAAGGCCTTGATGTCTACTACAAATCGTTCTATTGTGGTGTTTGAGGATATTGATTGCAGCAAGGGATCCGTAAACCGGAAATCAGCAGAGAAGAAGTTTATGGTTTCCGAAGCCAAGTTTACACTCTCGGGTCTACTGAATGTCGTCGATGGTCTGTGGTCAAGTTGCGGTGATGAGAGGATTATTGTGTTCACCACCAACCACAAGGAACGGCTAGACCCCGCATTGCTGCGGCCTGGTCGAATGGATATGCACATTCATATGTCGTATTGCACCCCTAGAGGGTTTAAGACCTTAGCCTTAAACTACCTTGGCATTCAGGACACCAGCGAACATCACCTGTGTCAAGAAATTGAAGGGTTGATGGAGAGCACAAACATCACCACCGCAGAGGTTTGTGAGGAGCTTATGAGGGGTGGGGGTGATACTGATGATGCTGATGTTGCTCTTGAAGGACTTGTCACTTGTCTGAAGCGTAAGAGGCTCGAGACCAACAAGGCAGAAGAAGAAGAGGTTGGGAAAGCAAAGAGGCAGAAACAGATTAATGCATCTGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

505

Amino Acids

57.45

Weight (kDa)

7.23

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 43 - 147 7.1e-16 Domain associated at C-terminal with AAA
AAA PF00004 279 - 408 3.2e-19 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 410 - 485 1.4e-18 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 222, 971, 1090
AccIII TCCGGA 1 cut(s) 251
AciI CCGC 7 cut(s) 291, 490, 881, 1125, 1179, 1189, 1344
AclI AACGTT 1 cut(s) 622
AclWI GGATC 2 cut(s) 1017, 1030
AcoI YGGCCR 1 cut(s) 882
AcsI RAATTY 1 cut(s) 25
AcuI CTGAAG 3 cut(s) 617, 770, 1448
AfaI GTAC 2 cut(s) 542, 859
AfiI CCNNNNNNNGG 6 cut(s) 86, 647, 680, 860, 1032, 1239
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 5 cut(s) 115, 166, 578, 1313, 1409
AjnI CCWGG 3 cut(s) 135, 186, 1192
AjuI GAANNNNNNNTTGG 2 cut(s) 1455, 1487
AluBI AGCT 6 cut(s) 200, 601, 716, 748, 873, 1363
AluI AGCT 6 cut(s) 200, 601, 716, 748, 873, 1363
Alw21I GWGCWC 2 cut(s) 711, 1331
Alw26I GTCTC 2 cut(s) 719, 1439
AlwI GGATC 2 cut(s) 1017, 1030
Ama87I CYCGRG 2 cut(s) 1084, 1442
Aor13HI TCCGGA 1 cut(s) 251
AoxI GGCC 5 cut(s) 69, 564, 882, 960, 1190
ApeKI GCWGC 2 cut(s) 1014, 1186
ApoI RAATTY 1 cut(s) 25
AseI ATTAAT 1 cut(s) 1500
AspA2I CCTAGG 1 cut(s) 509
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 3 cut(s) 190, 671, 848
AsuC2I CCSGG 1 cut(s) 855
AsuHPI GGTGA 8 cut(s) 197, 337, 394, 1139, 1141, 1286, 1330, 1391
AsuII TTCGAA 1 cut(s) 340
AvaI CYCGRG 2 cut(s) 1084, 1442
AvaII GGWCC 3 cut(s) 190, 671, 848
AvrII CCTAGG 1 cut(s) 509
BamHI GGATCC 1 cut(s) 1022
BarI GAAGNNNNNNTAC 2 cut(s) 818, 850
BauI CACGAG 1 cut(s) 645
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 711, 1331
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 2 cut(s) 1026, 1173
BccI CCATC 5 cut(s) 243, 471, 635, 1100, 1315
BceAI ACGGC 2 cut(s) 365, 1184
BciT130I CCWGG 3 cut(s) 137, 188, 1194
BclI TGATCA 1 cut(s) 759
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 2 cut(s) 719, 1439
BfaI CTAG 4 cut(s) 231, 510, 1172, 1236
BfmI CTRYAG 1 cut(s) 469
BglII AGATCT 1 cut(s) 700
BisI GCNGC 5 cut(s) 291, 882, 1015, 1187, 1190
BlnI CCTAGG 1 cut(s) 509
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 5 cut(s) 292, 883, 1016, 1188, 1191
Bme1390I CCNGG 4 cut(s) 137, 188, 855, 1194
Bme18I GGWCC 3 cut(s) 190, 671, 848
BmeT110I CYCGRG 2 cut(s) 1084, 1442
BmgT120I GGNCC 3 cut(s) 190, 671, 848
BmiI GGNNCC 3 cut(s) 192, 741, 1024
BmrFI CCNGG 4 cut(s) 137, 188, 855, 1194
BmsI GCATC 6 cut(s) 30, 183, 277, 571, 1381, 1514
BpiI GAAGAC 1 cut(s) 48
Bpu10I CCTNAGC 3 cut(s) 294, 561, 1252
Bpu1102I GCTNAGC 1 cut(s) 267
Bpu14I TTCGAA 1 cut(s) 340
BpuEI CTTGAG 2 cut(s) 180, 725
BpuMI CCSGG 1 cut(s) 855
BsaBI GATNNNNATC 1 cut(s) 462
BsaI GGTCTC 1 cut(s) 1439
BsaJI CCNNGG 5 cut(s) 509, 567, 735, 885, 1267
BsaWI WCCGGW 2 cut(s) 251, 1032
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 6 cut(s) 86, 647, 680, 860, 1032, 1239
Bse1I ACTGG 2 cut(s) 865, 930
Bse3DI GCAATG 3 cut(s) 57, 949, 1181
Bse8I GATNNNNATC 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 251
BseBI CCWGG 3 cut(s) 137, 188, 1194
BseDI CCNNGG 5 cut(s) 509, 567, 735, 885, 1267
BseGI GGATG 3 cut(s) 543, 627, 762
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 6 cut(s) 86, 647, 680, 860, 1032, 1239
BseMI GCAATG 3 cut(s) 57, 949, 1181
BseMII CTCAG 3 cut(s) 281, 285, 575
BseNI ACTGG 2 cut(s) 865, 930
BseRI GAGGAG 4 cut(s) 206, 238, 805, 1373
BseXI GCAGC 2 cut(s) 1026, 1173
BseYI CCCAGC 1 cut(s) 712
BshFI GGCC 5 cut(s) 71, 566, 884, 962, 1192
BsiHKAI GWGCWC 2 cut(s) 711, 1331
BsiHKCI CYCGRG 2 cut(s) 1084, 1442
BsiSI CCGG 4 cut(s) 252, 743, 855, 1033
BslI CCNNNNNNNGG 6 cut(s) 86, 647, 680, 860, 1032, 1239
BsmAI GTCTC 2 cut(s) 719, 1439
BsmI GAATGC 2 cut(s) 490, 1272
BsnI GGCC 5 cut(s) 71, 566, 884, 962, 1192
Bso31I GGTCTC 1 cut(s) 1439
BsoBI CYCGRG 2 cut(s) 1084, 1442
Bsp119I TTCGAA 1 cut(s) 340
Bsp1286I GDGCHC 2 cut(s) 711, 1331
Bsp13I TCCGGA 1 cut(s) 251
Bsp143I GATC 5 cut(s) 315, 441, 700, 759, 1022
Bsp1720I GCTNAGC 1 cut(s) 267
Bsp19I CCATGG 2 cut(s) 735, 885
BspACI CCGC 7 cut(s) 291, 490, 881, 1125, 1179, 1189, 1344
BspANI GGCC 5 cut(s) 71, 566, 884, 962, 1192
BspCNI CTCAG 3 cut(s) 280, 286, 574
BspEI TCCGGA 1 cut(s) 251
BspLI GGNNCC 3 cut(s) 192, 741, 1024
BspPI GGATC 2 cut(s) 1017, 1030
BspT104I TTCGAA 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 1439
BsrDI GCAATG 3 cut(s) 57, 949, 1181
BsrI ACTGG 2 cut(s) 865, 930
BssECI CCNNGG 5 cut(s) 509, 567, 735, 885, 1267
BssMI GATC 5 cut(s) 315, 441, 700, 759, 1022
BssSI CACGAG 1 cut(s) 645
BssT1I CCWWGG 4 cut(s) 509, 735, 885, 1267
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 3 cut(s) 137, 188, 1194
Bst4CI ACNGT 2 cut(s) 227, 473
Bst6I CTCTTC 4 cut(s) 746, 782, 821, 1461
BstBI TTCGAA 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 4 cut(s) 267, 294, 561, 1252
BstDSI CCRYGG 2 cut(s) 735, 885
BstF5I GGATG 3 cut(s) 543, 627, 762
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 5 cut(s) 318, 444, 703, 762, 1025
BstMAI GTCTC 2 cut(s) 719, 1439
BstMBI GATC 5 cut(s) 315, 441, 700, 759, 1022
BstMWI GCNNNNNNNGC 4 cut(s) 83, 887, 1400, 1438
BstNI CCWGG 3 cut(s) 137, 188, 1194
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 4 cut(s) 135, 186, 853, 1192
BstSFI CTRYAG 1 cut(s) 469
BstV1I GCAGC 2 cut(s) 1026, 1173
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 2 cut(s) 700, 1022
BstYI RGATCY 2 cut(s) 700, 1022
BsuRI GGCC 5 cut(s) 71, 566, 884, 962, 1192
BtgI CCRYGG 2 cut(s) 735, 885
BtgZI GCGATG 1 cut(s) 221
BtsCI GGATG 3 cut(s) 543, 627, 762
Cac8I GCNNGC 1 cut(s) 490
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 3 cut(s) 190, 671, 848
CsiI ACCWGGT 1 cut(s) 135
Csp6I GTAC 2 cut(s) 541, 858
CviAII CATG 4 cut(s) 94, 439, 736, 886
CviQI GTAC 2 cut(s) 541, 858
DdeI CTNAG 4 cut(s) 267, 294, 561, 1252
DpnI GATC 5 cut(s) 317, 443, 702, 761, 1024
DpnII GATC 5 cut(s) 315, 441, 700, 759, 1022
EaeI YGGCCR 1 cut(s) 882
Eam1104I CTCTTC 4 cut(s) 746, 782, 821, 1461
EarI CTCTTC 4 cut(s) 746, 782, 821, 1461
Eco130I CCWWGG 4 cut(s) 509, 735, 885, 1267
Eco147I AGGCCT 1 cut(s) 962
Eco31I GGTCTC 1 cut(s) 1439
Eco47I GGWCC 3 cut(s) 190, 671, 848
Eco57I CTGAAG 3 cut(s) 617, 770, 1448
Eco88I CYCGRG 2 cut(s) 1084, 1442
EcoRII CCWGG 3 cut(s) 135, 186, 1192
EcoT14I CCWWGG 4 cut(s) 509, 735, 885, 1267
EcoT22I ATGCAT 1 cut(s) 1507
ErhI CCWWGG 4 cut(s) 509, 735, 885, 1267
FaeI CATG 4 cut(s) 97, 442, 739, 889
FatI CATG 4 cut(s) 93, 438, 735, 885
FauI CCCGC 2 cut(s) 497, 1186
FauNDI CATATG 1 cut(s) 1218
FbaI TGATCA 1 cut(s) 759
FblI GTMKAC 3 cut(s) 222, 971, 1090
Fnu4HI GCNGC 5 cut(s) 291, 882, 1015, 1187, 1190
FokI GGATG 3 cut(s) 530, 614, 769
Fsp4HI GCNGC 5 cut(s) 291, 882, 1015, 1187, 1190
FspBI CTAG 4 cut(s) 231, 510, 1172, 1236
GlaI GCGC 1 cut(s) 272
GluI GCNGC 5 cut(s) 291, 882, 1015, 1187, 1190
GsaI CCCAGC 1 cut(s) 716
HaeIII GGCC 5 cut(s) 71, 566, 884, 962, 1192
HapII CCGG 4 cut(s) 252, 743, 855, 1033
HhaI GCGC 1 cut(s) 273
Hin1II CATG 4 cut(s) 97, 442, 739, 889
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HincII GTYRAC 2 cut(s) 223, 501
HindII GTYRAC 2 cut(s) 223, 501
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 4 cut(s) 255, 342, 536, 592
HpaI GTTAAC 1 cut(s) 501
HpaII CCGG 4 cut(s) 252, 743, 855, 1033
HphI GGTGA 8 cut(s) 197, 337, 394, 1139, 1141, 1286, 1330, 1391
Hpy166II GTNNAC 8 cut(s) 223, 501, 851, 972, 1030, 1077, 1091, 1149
Hpy188I TCNGA 5 cut(s) 324, 597, 1066, 1428, 1510
Hpy188III TCNNGA 8 cut(s) 252, 704, 724, 784, 1277, 1304, 1406, 1444
Hpy8I GTNNAC 8 cut(s) 223, 501, 851, 972, 1030, 1077, 1091, 1149
Hpy99I CGWCG 2 cut(s) 224, 1106
HpyAV CCTTC 5 cut(s) 91, 684, 952, 1307, 1403
HpyCH4III ACNGT 2 cut(s) 227, 473
HpyCH4IV ACGT 2 cut(s) 615, 622
HpyCH4V TGCA 6 cut(s) 31, 584, 1014, 1210, 1230, 1505
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 887, 1400, 1438
HpyF3I CTNAG 4 cut(s) 267, 294, 561, 1252
HpySE526I ACGT 2 cut(s) 615, 622
Hsp92II CATG 4 cut(s) 97, 442, 739, 889
HspAI GCGC 1 cut(s) 271
Kpn2I TCCGGA 1 cut(s) 251
Ksp22I TGATCA 1 cut(s) 759
KspAI GTTAAC 1 cut(s) 501
Kzo9I GATC 5 cut(s) 315, 441, 700, 759, 1022
LmnI GCTCC 4 cut(s) 108, 739, 745, 1360
Lsp1109I GCAGC 2 cut(s) 1026, 1173
LweI GCATC 6 cut(s) 30, 183, 277, 571, 1381, 1514
MabI ACCWGGT 1 cut(s) 135
MaeI CTAG 4 cut(s) 231, 510, 1172, 1236
MaeII ACGT 2 cut(s) 615, 622
MaeIII GTNAC 4 cut(s) 89, 382, 476, 1417
MalI GATC 5 cut(s) 317, 443, 702, 761, 1024
MboI GATC 5 cut(s) 315, 441, 700, 759, 1022
MfeI CAATTG 1 cut(s) 685
MflI RGATCY 2 cut(s) 700, 1022
MhlI GDGCHC 2 cut(s) 711, 1331
MluCI AATT 5 cut(s) 6, 25, 121, 685, 1308
MlyI GAGTC 2 cut(s) 530, 586
MmeI TCCRAC 1 cut(s) 513
Mph1103I ATGCAT 1 cut(s) 1507
MroI TCCGGA 1 cut(s) 251
MseI TTAA 8 cut(s) 9, 423, 500, 618, 906, 1245, 1259, 1500
MslI CAYNNNNRTG 3 cut(s) 50, 98, 1217
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 4 cut(s) 252, 743, 855, 1033
MspR9I CCNGG 4 cut(s) 137, 188, 855, 1194
MunI CAATTG 1 cut(s) 685
Mva1269I GAATGC 2 cut(s) 490, 1272
MvaI CCWGG 3 cut(s) 137, 188, 1194
MwoI GCNNNNNNNGC 4 cut(s) 83, 887, 1400, 1438
NciI CCSGG 1 cut(s) 855
NcoI CCATGG 2 cut(s) 735, 885
NdeI CATATG 1 cut(s) 1218
NdeII GATC 5 cut(s) 315, 441, 700, 759, 1022
NlaIII CATG 4 cut(s) 97, 442, 739, 889
NlaIV GGNNCC 3 cut(s) 192, 741, 1024
NmeAIII GCCGAG 2 cut(s) 47, 592
NmuCI GTSAC 2 cut(s) 476, 1417
NsiI ATGCAT 1 cut(s) 1507
NspI RCATGY 1 cut(s) 97
NspV TTCGAA 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 1442
PceI AGGCCT 1 cut(s) 962
PciI ACATGT 1 cut(s) 93
PctI GAATGC 2 cut(s) 490, 1272
PfeI GAWTC 2 cut(s) 255, 342
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 5 cut(s) 292, 883, 1016, 1188, 1191
PleI GAGTC 2 cut(s) 530, 586
PpsI GAGTC 2 cut(s) 530, 586
PscI ACATGT 1 cut(s) 93
PshBI ATTAAT 1 cut(s) 1500
Psp1406I AACGTT 1 cut(s) 622
Psp6I CCWGG 3 cut(s) 135, 186, 1192
PspFI CCCAGC 1 cut(s) 712
PspGI CCWGG 3 cut(s) 135, 186, 1192
PspN4I GGNNCC 3 cut(s) 192, 741, 1024
PspPI GGNCC 3 cut(s) 190, 671, 848
PsuI RGATCY 2 cut(s) 700, 1022
RsaI GTAC 2 cut(s) 542, 859
RsaNI GTAC 2 cut(s) 541, 858
RseI CAYNNNNRTG 3 cut(s) 50, 98, 1217
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 8 cut(s) 9, 423, 500, 618, 906, 1245, 1259, 1500
SatI GCNGC 5 cut(s) 291, 882, 1015, 1187, 1190
Sau3AI GATC 5 cut(s) 315, 441, 700, 759, 1022
Sau96I GGNCC 3 cut(s) 190, 671, 848
SchI GAGTC 2 cut(s) 530, 586
ScrFI CCNGG 4 cut(s) 137, 188, 855, 1194
SduI GDGCHC 2 cut(s) 711, 1331
SexAI ACCWGGT 1 cut(s) 135
SfaNI GCATC 6 cut(s) 30, 183, 277, 571, 1381, 1514
SfcI CTRYAG 1 cut(s) 469
Sfr274I CTCGAG 1 cut(s) 1442
SfuI TTCGAA 1 cut(s) 340
SinI GGWCC 3 cut(s) 190, 671, 848
SlaI CTCGAG 1 cut(s) 1442
SmiMI CAYNNNNRTG 3 cut(s) 50, 98, 1217
SmlI CTYRAG 3 cut(s) 195, 704, 1442
SmoI CTYRAG 3 cut(s) 195, 704, 1442
Sse9I AATT 5 cut(s) 6, 25, 121, 685, 1308
SseBI AGGCCT 1 cut(s) 962
SsiI CCGC 7 cut(s) 291, 490, 881, 1125, 1179, 1189, 1344
SspMI CTAG 4 cut(s) 231, 510, 1172, 1236
StuI AGGCCT 1 cut(s) 962
StyD4I CCNGG 4 cut(s) 135, 186, 853, 1192
StyI CCWWGG 4 cut(s) 509, 735, 885, 1267
TaaI ACNGT 2 cut(s) 227, 473
TaiI ACGT 2 cut(s) 618, 625
TaqI TCGA 6 cut(s) 222, 340, 723, 1104, 1198, 1443
TasI AATT 5 cut(s) 6, 25, 121, 685, 1308
TauI GCSGC 3 cut(s) 293, 884, 1192
TfiI GAWTC 2 cut(s) 255, 342
Tru1I TTAA 8 cut(s) 9, 423, 500, 618, 906, 1245, 1259, 1500
Tru9I TTAA 8 cut(s) 9, 423, 500, 618, 906, 1245, 1259, 1500
TseFI GTSAC 2 cut(s) 476, 1417
TseI GCWGC 2 cut(s) 1014, 1186
Tsp45I GTSAC 2 cut(s) 476, 1417
TspDTI ATGAA 2 cut(s) 115, 1205
TspGWI ACGGA 3 cut(s) 437, 684, 1015
VpaK11BI GGWCC 3 cut(s) 190, 671, 848
VspI ATTAAT 1 cut(s) 1500
XapI RAATTY 1 cut(s) 25
XceI RCATGY 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 1442
XmaJI CCTAGG 1 cut(s) 509
XmiI GTMKAC 3 cut(s) 222, 971, 1090
XspI CTAG 4 cut(s) 231, 510, 1172, 1236
Zsp2I ATGCAT 1 cut(s) 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.