Rh2BG135700

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003681
Physical Location & Seq
Reverse (-)
11584083 .. 11585821
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG135700.1

Sequence Viewer

Length: 1095 bp
ATGGAGGAGCAATATTATGATGACCACAGCAATTCCAATTTCAAAGATATAATGCCCACAGCTATATCCTCCTTTTTCTCGGCCTATGCCCCCTTTGCTGGTTACATGTTTATGCTCCAATCCATGAACACCAATTTCCTCCCTTACGAGTTTAACTCATACGTATACTCGTACTTAAAATCAAGCCTCAGCTACCTCTTGGACCCTCTAGCCAATCACATAACTCTCCTCATCGACAACTCTAGCGATGAAGACTACTCGGACGAAATCTATGATTCTGCTGAGGTCTATCTTTCGACCAAGATCAACCCGAAAACCCAAATCTTTCGAATTAGCAAAGCACCGAAACAAAAGGTCCCCATCCTTGGTGTTACCACCAACCAAGAAATCACTGACACTTTCGAAGACATTAACGTGAAGTGGCGGATGATCTTCCCTAAAGAAAACGATGGTTACTACAGTAGCGTCTATAAGAGCCGCATGGAGCTAACATTCCACAGGAAACACAAAGACAAAGTTATAAACTCGTACATCCCCTATGTGTTGGCTCGGGCCAAGGCTATCAAAGATGCAGAAAAGACTCTGAAGCTTTATAGTTACAATAGTAAACGTTCCTCGGTCTTCAAAATTTCTCGTGGACGCTCTCATGGTTCTGATGACGATGATGACTTTGCACCTTCTTCGGGCAAATGGAGTTGCATCGATCTTGAGCACCCGGCTACTTTTGAGACAATGGCAATGGAGCCGGAGCTGAAGAGGATGATCATAGAGGATTTGGACAACAAGGGATCCGTAAACCGGGAATCAGCAGAGAAGAAGTCTATGGTTTCCGAAGCCAAGGACACCAGCGAACATCGCCTGTGTGGAGAAATTGAAGCATTGGTGGAGAGCACTAACATCACCCCTGCAGAGGTTTGTGAGGAGCTTATGAGGGGTGGGGGTGATGGTGATGAAGCCGATGCTGCTCTTGAAGGACTTGTCAATTGTCTCAAGCGTAAGAGGCTCGAGACCAACAAGATAGAGGATGAAGAGGTTAGGAAAGCAAAGAGGCAGAAAACAGATTATAATGCATCTGAAATTTCTGAAAATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

364

Amino Acids

41.76

Weight (kDa)

5.08

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 48 - 143 2.1e-15 Domain associated at C-terminal with AAA
AAA_lid_At3g28540 PF25568 281 - 337 1.8e-12 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 521, 1065
AccI GTMKAC 1 cut(s) 165
AciI CCGC 2 cut(s) 424, 478
AclI AACGTT 1 cut(s) 610
AclWI GGATC 2 cut(s) 783, 796
AcsI RAATTY 2 cut(s) 627, 1077
AcuI CTGAAG 2 cut(s) 605, 773
AfaI GTAC 2 cut(s) 173, 530
AfiI CCNNNNNNNGG 5 cut(s) 98, 365, 683, 798, 910
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 4 cut(s) 43, 625, 875, 971
AluBI AGCT 6 cut(s) 62, 192, 487, 589, 751, 925
AluI AGCT 6 cut(s) 62, 192, 487, 589, 751, 925
Alw21I GWGCWC 2 cut(s) 714, 893
Alw26I GTCTC 3 cut(s) 722, 992, 1001
AlwI GGATC 2 cut(s) 783, 796
Ama87I CYCGRG 2 cut(s) 549, 1004
AoxI GGCC 2 cut(s) 81, 552
ApeKI GCWGC 1 cut(s) 962
ApoI RAATTY 2 cut(s) 627, 1077
AspS9I GGNCC 3 cut(s) 202, 355, 552
AsuC2I CCSGG 2 cut(s) 716, 800
AsuHPI GGTGA 3 cut(s) 892, 953, 959
AsuII TTCGAA 2 cut(s) 328, 402
AvaI CYCGRG 2 cut(s) 549, 1004
AvaII GGWCC 2 cut(s) 202, 355
BamHI GGATCC 1 cut(s) 788
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 3 cut(s) 258, 411, 613
Bbv12I GWGCWC 2 cut(s) 714, 893
BbvCI CCTCAGC 2 cut(s) 188, 282
BbvI GCAGC 1 cut(s) 949
BccI CCATC 3 cut(s) 368, 443, 938
BcgI CGANNNNNNTGC 2 cut(s) 663, 697
BclI TGATCA 1 cut(s) 762
BcnI CCSGG 2 cut(s) 716, 800
BcoDI GTCTC 3 cut(s) 722, 992, 1001
BfaI CTAG 2 cut(s) 209, 243
BfmI CTRYAG 2 cut(s) 457, 906
BisI GCNGC 2 cut(s) 478, 963
BlsI GCNGC 2 cut(s) 479, 964
Bme1390I CCNGG 2 cut(s) 716, 800
Bme18I GGWCC 2 cut(s) 202, 355
BmeT110I CYCGRG 2 cut(s) 549, 1004
BmgT120I GGNCC 3 cut(s) 202, 355, 552
BmiI GGNNCC 4 cut(s) 204, 357, 744, 790
BmrFI CCNGG 2 cut(s) 716, 800
BmsI GCATC 4 cut(s) 559, 708, 949, 1079
BpiI GAAGAC 3 cut(s) 258, 411, 613
BplI GAGNNNNNCTC 2 cut(s) 140, 172
Bpu10I CCTNAGC 2 cut(s) 188, 282
Bpu14I TTCGAA 2 cut(s) 328, 402
BpuEI CTTGAG 2 cut(s) 728, 974
BpuMI CCSGG 2 cut(s) 716, 800
Bsa29I ATCGAT 1 cut(s) 702
BsaAI YACGTR 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 1001
BsaJI CCNNGG 4 cut(s) 364, 555, 615, 837
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 365, 683, 798, 910
Bse3DI GCAATG 1 cut(s) 744
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 4 cut(s) 364, 555, 615, 837
BseGI GGATG 5 cut(s) 360, 432, 531, 765, 1030
BseLI CCNNNNNNNGG 5 cut(s) 98, 365, 683, 798, 910
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 2 cut(s) 202, 273
BseRI GAGGAG 3 cut(s) 20, 218, 935
BseXI GCAGC 1 cut(s) 949
BshFI GGCC 2 cut(s) 83, 554
BshVI ATCGAT 1 cut(s) 702
BsiHKAI GWGCWC 2 cut(s) 714, 893
BsiHKCI CYCGRG 2 cut(s) 549, 1004
BsiSI CCGG 3 cut(s) 716, 746, 799
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 5 cut(s) 98, 365, 683, 798, 910
BsmAI GTCTC 3 cut(s) 722, 992, 1001
BsmFI GGGAC 1 cut(s) 341
BsnI GGCC 2 cut(s) 83, 554
Bso31I GGTCTC 1 cut(s) 1001
BsoBI CYCGRG 2 cut(s) 549, 1004
Bsp119I TTCGAA 2 cut(s) 328, 402
Bsp1286I GDGCHC 2 cut(s) 714, 893
Bsp143I GATC 5 cut(s) 303, 429, 703, 762, 788
BspACI CCGC 2 cut(s) 424, 478
BspANI GGCC 2 cut(s) 83, 554
BspCNI CTCAG 2 cut(s) 201, 274
BspDI ATCGAT 1 cut(s) 702
BspLI GGNNCC 4 cut(s) 204, 357, 744, 790
BspMAI CTGCAG 1 cut(s) 910
BspPI GGATC 2 cut(s) 783, 796
BspT104I TTCGAA 2 cut(s) 328, 402
BspTNI GGTCTC 1 cut(s) 1001
BsrDI GCAATG 1 cut(s) 744
BssECI CCNNGG 4 cut(s) 364, 555, 615, 837
BssMI GATC 5 cut(s) 303, 429, 703, 762, 788
BssNAI GTATAC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 3 cut(s) 364, 555, 837
Bst1107I GTATAC 1 cut(s) 166
Bst2BI CACGAG 1 cut(s) 633
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 2 cut(s) 749, 1023
BstBAI YACGTR 1 cut(s) 163
BstBI TTCGAA 2 cut(s) 328, 402
BstDEI CTNAG 2 cut(s) 188, 282
BstF5I GGATG 5 cut(s) 360, 432, 531, 765, 1030
BstKTI GATC 5 cut(s) 306, 432, 706, 765, 791
BstMAI GTCTC 3 cut(s) 722, 992, 1001
BstMBI GATC 5 cut(s) 303, 429, 703, 762, 788
BstMWI GCNNNNNNNGC 4 cut(s) 95, 855, 962, 1000
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 2 cut(s) 714, 798
BstSFI CTRYAG 2 cut(s) 457, 906
BstSNI TACGTA 1 cut(s) 163
BstV1I GCAGC 1 cut(s) 949
BstV2I GAAGAC 3 cut(s) 258, 411, 613
BstX2I RGATCY 1 cut(s) 788
BstYI RGATCY 1 cut(s) 788
BstZ17I GTATAC 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 702
BsuRI GGCC 2 cut(s) 83, 554
BsuTUI ATCGAT 1 cut(s) 702
BtgZI GCGATG 2 cut(s) 261, 839
BtsCI GGATG 5 cut(s) 360, 432, 531, 765, 1030
BtsIMutI CAGTG 1 cut(s) 390
Cfr13I GGNCC 3 cut(s) 202, 355, 552
ClaI ATCGAT 1 cut(s) 702
CseI GACGC 2 cut(s) 454, 648
Csp6I GTAC 2 cut(s) 172, 529
CviAII CATG 4 cut(s) 106, 124, 481, 647
CviQI GTAC 2 cut(s) 172, 529
DdeI CTNAG 2 cut(s) 188, 282
DpnI GATC 5 cut(s) 305, 431, 705, 764, 790
DpnII GATC 5 cut(s) 303, 429, 703, 762, 788
Eam1104I CTCTTC 2 cut(s) 749, 1023
EarI CTCTTC 2 cut(s) 749, 1023
EciI GGCGGA 1 cut(s) 439
Eco105I TACGTA 1 cut(s) 163
Eco130I CCWWGG 3 cut(s) 364, 555, 837
Eco31I GGTCTC 1 cut(s) 1001
Eco47I GGWCC 2 cut(s) 202, 355
Eco57I CTGAAG 2 cut(s) 605, 773
Eco88I CYCGRG 2 cut(s) 549, 1004
EcoO109I RGGNCCY 1 cut(s) 355
EcoT14I CCWWGG 3 cut(s) 364, 555, 837
EcoT22I ATGCAT 1 cut(s) 1072
ErhI CCWWGG 3 cut(s) 364, 555, 837
FaeI CATG 4 cut(s) 109, 127, 484, 650
FaqI GGGAC 1 cut(s) 341
FatI CATG 4 cut(s) 105, 123, 480, 646
FbaI TGATCA 1 cut(s) 762
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 2 cut(s) 478, 963
FokI GGATG 5 cut(s) 347, 439, 518, 772, 1037
Fsp4HI GCNGC 2 cut(s) 478, 963
FspBI CTAG 2 cut(s) 209, 243
GluI GCNGC 2 cut(s) 478, 963
HaeIII GGCC 2 cut(s) 83, 554
HapII CCGG 3 cut(s) 716, 746, 799
HgaI GACGC 2 cut(s) 454, 648
Hin1II CATG 4 cut(s) 109, 127, 484, 650
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 3 cut(s) 275, 580, 803
HpaII CCGG 3 cut(s) 716, 746, 799
HphI GGTGA 3 cut(s) 892, 953, 959
Hpy166II GTNNAC 4 cut(s) 166, 608, 638, 796
Hpy188I TCNGA 6 cut(s) 262, 585, 655, 832, 1075, 1084
Hpy188III TCNNGA 3 cut(s) 707, 968, 1006
Hpy8I GTNNAC 4 cut(s) 166, 608, 638, 796
HpyAV CCTTC 2 cut(s) 687, 965
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 3 cut(s) 162, 414, 610
HpyCH4V TGCA 5 cut(s) 572, 674, 699, 908, 1070
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 855, 962, 1000
HpyF3I CTNAG 2 cut(s) 188, 282
HpySE526I ACGT 3 cut(s) 162, 414, 610
Hsp92II CATG 4 cut(s) 109, 127, 484, 650
Ksp22I TGATCA 1 cut(s) 762
Kzo9I GATC 5 cut(s) 303, 429, 703, 762, 788
LmnI GCTCC 6 cut(s) 7, 120, 484, 742, 748, 922
LpnPI CCDG 8 cut(s) 84, 484, 729, 759, 812, 859, 872, 918
Lsp1109I GCAGC 1 cut(s) 949
LweI GCATC 4 cut(s) 559, 708, 949, 1079
MaeI CTAG 2 cut(s) 209, 243
MaeII ACGT 3 cut(s) 162, 414, 610
MaeIII GTNAC 4 cut(s) 101, 370, 452, 596
MalI GATC 5 cut(s) 305, 431, 705, 764, 790
MboI GATC 5 cut(s) 303, 429, 703, 762, 788
MboII GAAGA 8 cut(s) 263, 416, 424, 613, 672, 766, 826, 1040
MfeI CAATTG 1 cut(s) 982
MflI RGATCY 1 cut(s) 788
MhlI GDGCHC 2 cut(s) 714, 893
MluCI AATT 9 cut(s) 31, 37, 133, 330, 627, 870, 982, 1077, 1087
MlyI GAGTC 1 cut(s) 574
Mph1103I ATGCAT 1 cut(s) 1072
MseI TTAA 3 cut(s) 153, 176, 411
MslI CAYNNNNRTG 2 cut(s) 110, 413
MspI CCGG 3 cut(s) 716, 746, 799
MspR9I CCNGG 2 cut(s) 716, 800
MunI CAATTG 1 cut(s) 982
MwoI GCNNNNNNNGC 4 cut(s) 95, 855, 962, 1000
NciI CCSGG 2 cut(s) 716, 800
NdeII GATC 5 cut(s) 303, 429, 703, 762, 788
NlaIII CATG 4 cut(s) 109, 127, 484, 650
NlaIV GGNNCC 4 cut(s) 204, 357, 744, 790
NmeAIII GCCGAG 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 1072
NspI RCATGY 1 cut(s) 109
NspV TTCGAA 2 cut(s) 328, 402
PaeR7I CTCGAG 1 cut(s) 1004
PciI ACATGT 1 cut(s) 105
PfeI GAWTC 2 cut(s) 275, 803
PkrI GCNGC 2 cut(s) 479, 964
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
Ppu21I YACGTR 1 cut(s) 163
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 1 cut(s) 105
PsiI TTATAA 2 cut(s) 521, 1065
Psp1406I AACGTT 1 cut(s) 610
Psp5II RGGWCCY 1 cut(s) 355
PspN4I GGNNCC 4 cut(s) 204, 357, 744, 790
PspPI GGNCC 3 cut(s) 202, 355, 552
PspPPI RGGWCCY 1 cut(s) 355
PstI CTGCAG 1 cut(s) 910
PsuI RGATCY 1 cut(s) 788
RsaI GTAC 2 cut(s) 173, 530
RsaNI GTAC 2 cut(s) 172, 529
RseI CAYNNNNRTG 2 cut(s) 110, 413
SaqAI TTAA 3 cut(s) 153, 176, 411
SatI GCNGC 2 cut(s) 478, 963
Sau3AI GATC 5 cut(s) 303, 429, 703, 762, 788
Sau96I GGNCC 3 cut(s) 202, 355, 552
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 2 cut(s) 716, 800
SduI GDGCHC 2 cut(s) 714, 893
SfaNI GCATC 4 cut(s) 559, 708, 949, 1079
SfcI CTRYAG 2 cut(s) 457, 906
Sfr274I CTCGAG 1 cut(s) 1004
SfuI TTCGAA 2 cut(s) 328, 402
SinI GGWCC 2 cut(s) 202, 355
SlaI CTCGAG 1 cut(s) 1004
SmiMI CAYNNNNRTG 2 cut(s) 110, 413
SmlI CTYRAG 3 cut(s) 707, 989, 1004
SmoI CTYRAG 3 cut(s) 707, 989, 1004
SnaBI TACGTA 1 cut(s) 163
Sse9I AATT 9 cut(s) 31, 37, 133, 330, 627, 870, 982, 1077, 1087
SsiI CCGC 2 cut(s) 424, 478
SspI AATATT 1 cut(s) 14
SspMI CTAG 2 cut(s) 209, 243
StyD4I CCNGG 2 cut(s) 714, 798
StyI CCWWGG 3 cut(s) 364, 555, 837
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 3 cut(s) 165, 417, 613
TaqI TCGA 6 cut(s) 234, 296, 328, 402, 702, 1005
TaqII GACCGA 1 cut(s) 607
TasI AATT 9 cut(s) 31, 37, 133, 330, 627, 870, 982, 1077, 1087
TauI GCSGC 1 cut(s) 480
TfiI GAWTC 2 cut(s) 275, 803
Tru1I TTAA 3 cut(s) 153, 176, 411
Tru9I TTAA 3 cut(s) 153, 176, 411
TscAI CASTG 1 cut(s) 397
TseI GCWGC 1 cut(s) 962
TspDTI ATGAA 4 cut(s) 140, 264, 966, 1041
TspGWI ACGGA 1 cut(s) 781
TspRI CASTG 1 cut(s) 397
VpaK11BI GGWCC 2 cut(s) 202, 355
XapI RAATTY 2 cut(s) 627, 1077
XceI RCATGY 1 cut(s) 109
XhoI CTCGAG 1 cut(s) 1004
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 2 cut(s) 209, 243
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.