FvH4_1g12133

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003681
Physical Location & Seq
Reverse (-)
6631169 .. 6633186
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12133.t1

Sequence Viewer

Length: 1518 bp
ATGGTTTCTCCGAATCTCCAAGGTTCGATGCCCACAGCAATGTCTTCCTTCTTCTCGGCCTATGCCCCCTTTGCTGGTTACATGTTTATGCTCCAATCCATGAGAACCAACTTCATACCTCACCAGGTCCAAGCATACATACACAAATACATAAAATCATGCATCAACCATCTCTTGGATCCTCAAAGCAATCACATAACTCTCCTCGTCGACGGTTCTAGCAAACGCGACTACAACGGATTGCGAATGCAAAACCAAGTCTATGATGCTGCTTTGGTCTATCTCACCACCAAGATCAATCCGAAAACCAACCTTTTTCGTATCATCCAAGCACCTACACAAAAGACTCCCAAACTTGCTGTTGCCACAGACCAAGAAATTATCGACACTTTCGAAAACATAAGAGTAAAGTGGCGCATGATCTTCTCTTCCACTTCCACAAGTGATAGTGCCCGGATTGAGCTAACATTCCACAGAAAACACAAAGATAAGGTGATCGACTCGTACATCCCTTATGTGTTGGCTCAGGCTCAAGCTATTAAAGAGGCAGACAAGACTCTGAAGCTTTATAGTTACAACGTTAAACGTAGTAATTCCTATCAATACTCCCCTACTAACGACACGGTCCAAAGACCTAGTAGCAGCTTTGAATCTTCCGGCAGTTGGAGTTGCGTGGACCTTGAGCACCCTGCTACTTTCGATACAATAGCAATGGAGCCGGAGCTGAAGAGAATGATCATAGAGGATTTGGACAAGTTTGTGAAGAGGAGGGAGTTCTACAAGAGAGTTGGCAAGGCTTGGAAGAGAGGCTACTTGTTGTTTGGTCCACCCGGTACTGGAAAATCCAGCTTGGTTGCAGCCATGGCTAATCATCTCCATTTTGATATTTATGACTTGGAACTCACTAGTATTTCCAGCAACGATCAATTGAGGAAGGCCTTGGGGTCTACTACGAATCGTTCTATTGTGGTGTTTGAGGATATTGATTGCAGCAAGGAATCTGTAAACCGGGAATCAGCAGAGAGCAAGAGCTTTATGCAGATGTTTTCGGATGCTGAATCCAAGTTTACACTCTCAGGTCTACTGAATGTAGTCGATGGTCTGGGGTCAAGTTCCGGAGATGAGAGGATTATAGTGTTCACCACCAACCACAAGGAGCGGCTAGACCCTGCATTGTTGAGGCCTGGTCGAATGGACATGCACATTCACATGTCTTACTGCACCCCAAAAGGGTTCAAGACCTTAGCCTCAAACTACCTTGGCATTCAGGACACCAACGAACATCACCTGTGTAGAGAAGCTGAAGCATTGATGGAGAGCACAAACATCACCCCTGCAGAGGTTTGTGAGGAGCTTATGAGGGGTGGGGGTGATAGTGCTGATGATGCTCTTGAAGGACTTGTCAATTGCCTCAAGCGTAAGAGACTCGAGAGCTGCAAAATAGAGGAAGAAGAAATGGGAAAATCAAAGAGGCAGAAGCCAGATAACAATGCTTCTGAAATTTCCGAGAATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

506

Amino Acids

57.21

Weight (kDa)

6.93

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 39 - 140 1.8e-14 Domain associated at C-terminal with AAA
AAA PF00004 271 - 405 1.4e-18 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 407 - 477 5.1e-19 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 175
AccBSI CCGCTC 1 cut(s) 1159
AccI GTMKAC 3 cut(s) 210, 947, 1081
AccII CGCG 1 cut(s) 228
AccIII TCCGGA 1 cut(s) 1115
AciI CCGC 1 cut(s) 1159
AclI AACGTT 1 cut(s) 579
AclWI GGATC 2 cut(s) 173, 186
AcsI RAATTY 1 cut(s) 1500
AcuI CTGAAG 3 cut(s) 581, 746, 1323
AfaI GTAC 2 cut(s) 506, 835
AfiI CCNNNNNNNGG 7 cut(s) 74, 175, 663, 836, 1230, 1339, 1512
AflIII ACRYGT 2 cut(s) 81, 1209
AgsI TTSAA 3 cut(s) 650, 1237, 1394
AhlI ACTAGT 1 cut(s) 905
AjnI CCWGG 2 cut(s) 123, 1183
Alw21I GWGCWC 2 cut(s) 687, 1322
Alw26I GTCTC 1 cut(s) 1417
AlwI GGATC 2 cut(s) 173, 186
Ama87I CYCGRG 1 cut(s) 1427
Aor13HI TCCGGA 1 cut(s) 1115
AoxI GGCC 3 cut(s) 57, 936, 1181
ApeKI GCWGC 5 cut(s) 269, 642, 857, 990, 1434
ApoI RAATTY 1 cut(s) 1500
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 4 cut(s) 127, 625, 676, 824
AsuC2I CCSGG 3 cut(s) 454, 831, 1010
AsuHPI GGTGA 7 cut(s) 113, 277, 505, 1132, 1277, 1321, 1382
AsuII TTCGAA 1 cut(s) 393
AvaI CYCGRG 1 cut(s) 1427
AvaII GGWCC 4 cut(s) 127, 625, 676, 824
BaeGI GKGCMC 1 cut(s) 454
BamHI GGATCC 1 cut(s) 178
BarI GAAGNNNNNNTAC 2 cut(s) 794, 826
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 2 cut(s) 687, 1322
BbvI GCAGC 5 cut(s) 256, 654, 869, 1002, 1421
BccI CCATC 3 cut(s) 177, 1091, 1306
BciT130I CCWGG 2 cut(s) 125, 1185
BclI TGATCA 1 cut(s) 735
BcnI CCSGG 3 cut(s) 454, 831, 1010
BcoDI GTCTC 1 cut(s) 1417
BcuI ACTAGT 1 cut(s) 905
BfaI CTAG 4 cut(s) 219, 636, 906, 1163
BfmI CTRYAG 1 cut(s) 1335
BisI GCNGC 6 cut(s) 270, 643, 858, 991, 1160, 1435
BlsI GCNGC 6 cut(s) 271, 644, 859, 992, 1161, 1436
Bme1390I CCNGG 5 cut(s) 125, 454, 831, 1010, 1185
Bme18I GGWCC 4 cut(s) 127, 625, 676, 824
BmeT110I CYCGRG 1 cut(s) 1427
BmgT120I GGNCC 4 cut(s) 127, 625, 676, 824
BmiI GGNNCC 2 cut(s) 180, 717
BmrFI CCNGG 5 cut(s) 125, 454, 831, 1010, 1185
BmsI GCATC 5 cut(s) 18, 171, 256, 1042, 1375
BpiI GAAGAC 1 cut(s) 36
Bpu10I CCTNAGC 2 cut(s) 525, 1243
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 3 cut(s) 516, 701, 1397
BpuMI CCSGG 3 cut(s) 454, 831, 1010
BsaJI CCNNGG 4 cut(s) 19, 861, 939, 1258
BsaWI WCCGGW 1 cut(s) 1115
Bsc4I CCNNNNNNNGG 7 cut(s) 74, 175, 663, 836, 1230, 1339, 1512
Bse1I ACTGG 1 cut(s) 841
Bse3DI GCAATG 2 cut(s) 45, 717
BseAI TCCGGA 1 cut(s) 1115
BseBI CCWGG 2 cut(s) 125, 1185
BseDI CCNNGG 4 cut(s) 19, 861, 939, 1258
BseGI GGATG 3 cut(s) 324, 507, 1057
BseLI CCNNNNNNNGG 7 cut(s) 74, 175, 663, 836, 1230, 1339, 1512
BseMI GCAATG 2 cut(s) 45, 717
BseMII CTCAG 2 cut(s) 539, 1089
BseNI ACTGG 1 cut(s) 841
BseRI GAGGAG 3 cut(s) 194, 781, 1364
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 5 cut(s) 256, 654, 869, 1002, 1421
BsgI GTGCAG 1 cut(s) 1204
Bsh1236I CGCG 1 cut(s) 228
BshFI GGCC 3 cut(s) 59, 938, 1183
BsiHKAI GWGCWC 2 cut(s) 687, 1322
BsiHKCI CYCGRG 1 cut(s) 1427
BsiSI CCGG 6 cut(s) 454, 657, 719, 831, 1009, 1116
BslI CCNNNNNNNGG 7 cut(s) 74, 175, 663, 836, 1230, 1339, 1512
BsmAI GTCTC 1 cut(s) 1417
BsmI GAATGC 2 cut(s) 252, 1263
BsnI GGCC 3 cut(s) 59, 938, 1183
BsoBI CYCGRG 1 cut(s) 1427
Bsp119I TTCGAA 1 cut(s) 393
Bsp1286I GDGCHC 3 cut(s) 454, 687, 1322
Bsp13I TCCGGA 1 cut(s) 1115
Bsp143I GATC 6 cut(s) 178, 294, 420, 495, 735, 922
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 1 cut(s) 1159
BspANI GGCC 3 cut(s) 59, 938, 1183
BspCNI CTCAG 2 cut(s) 538, 1088
BspEI TCCGGA 1 cut(s) 1115
BspFNI CGCG 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 180, 717
BspMAI CTGCAG 1 cut(s) 1339
BspPI GGATC 2 cut(s) 173, 186
BspT104I TTCGAA 1 cut(s) 393
BsrBI CCGCTC 1 cut(s) 1159
BsrDI GCAATG 2 cut(s) 45, 717
BsrI ACTGG 1 cut(s) 841
BssECI CCNNGG 4 cut(s) 19, 861, 939, 1258
BssMI GATC 6 cut(s) 178, 294, 420, 495, 735, 922
BssT1I CCWWGG 4 cut(s) 19, 861, 939, 1258
Bst2UI CCWGG 2 cut(s) 125, 1185
Bst4CI ACNGT 2 cut(s) 215, 625
Bst6I CTCTTC 4 cut(s) 433, 722, 758, 797
BstBI TTCGAA 1 cut(s) 393
BstDEI CTNAG 3 cut(s) 525, 1075, 1243
BstDSI CCRYGG 1 cut(s) 861
BstF5I GGATG 3 cut(s) 324, 507, 1057
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 6 cut(s) 181, 297, 423, 498, 738, 925
BstMAI GTCTC 1 cut(s) 1417
BstMBI GATC 6 cut(s) 178, 294, 420, 495, 735, 922
BstMWI GCNNNNNNNGC 3 cut(s) 71, 863, 1385
BstNI CCWGG 2 cut(s) 125, 1185
BstNSI RCATGY 3 cut(s) 85, 1201, 1213
BstSCI CCNGG 5 cut(s) 123, 452, 829, 1008, 1183
BstSFI CTRYAG 1 cut(s) 1335
BstSLI GKGCMC 1 cut(s) 454
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 5 cut(s) 256, 654, 869, 1002, 1421
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 3 cut(s) 59, 938, 1183
BtgI CCRYGG 1 cut(s) 861
BtsCI GGATG 3 cut(s) 324, 507, 1057
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 4 cut(s) 127, 625, 676, 824
CsiI ACCWGGT 1 cut(s) 123
Csp6I GTAC 2 cut(s) 505, 834
CviAII CATG 7 cut(s) 82, 100, 159, 418, 862, 1198, 1210
CviQI GTAC 2 cut(s) 505, 834
DdeI CTNAG 3 cut(s) 525, 1075, 1243
DpnI GATC 6 cut(s) 180, 296, 422, 497, 737, 924
DpnII GATC 6 cut(s) 178, 294, 420, 495, 735, 922
Eam1104I CTCTTC 4 cut(s) 433, 722, 758, 797
EarI CTCTTC 4 cut(s) 433, 722, 758, 797
Eco130I CCWWGG 4 cut(s) 19, 861, 939, 1258
Eco147I AGGCCT 2 cut(s) 938, 1183
Eco47I GGWCC 4 cut(s) 127, 625, 676, 824
Eco57I CTGAAG 3 cut(s) 581, 746, 1323
Eco88I CYCGRG 1 cut(s) 1427
EcoRII CCWGG 2 cut(s) 123, 1183
EcoT14I CCWWGG 4 cut(s) 19, 861, 939, 1258
EcoT22I ATGCAT 1 cut(s) 164
ErhI CCWWGG 4 cut(s) 19, 861, 939, 1258
FaeI CATG 7 cut(s) 85, 103, 162, 421, 865, 1201, 1213
FatI CATG 7 cut(s) 81, 99, 158, 417, 861, 1197, 1209
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 3 cut(s) 210, 947, 1081
Fnu4HI GCNGC 6 cut(s) 270, 643, 858, 991, 1160, 1435
FokI GGATG 3 cut(s) 311, 494, 1064
Fsp4HI GCNGC 6 cut(s) 270, 643, 858, 991, 1160, 1435
FspBI CTAG 4 cut(s) 219, 636, 906, 1163
GlaI GCGC 1 cut(s) 416
GluI GCNGC 6 cut(s) 270, 643, 858, 991, 1160, 1435
HaeIII GGCC 3 cut(s) 59, 938, 1183
HapII CCGG 6 cut(s) 454, 657, 719, 831, 1009, 1116
HhaI GCGC 1 cut(s) 417
Hin1II CATG 7 cut(s) 85, 103, 162, 421, 865, 1201, 1213
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 563
HpaII CCGG 6 cut(s) 454, 657, 719, 831, 1009, 1116
HphI GGTGA 7 cut(s) 113, 277, 505, 1132, 1277, 1321, 1382
Hpy166II GTNNAC 8 cut(s) 211, 676, 827, 948, 1006, 1068, 1082, 1140
Hpy188I TCNGA 6 cut(s) 12, 303, 561, 1051, 1498, 1507
Hpy188III TCNNGA 5 cut(s) 1116, 1237, 1268, 1391, 1429
Hpy8I GTNNAC 8 cut(s) 211, 676, 827, 948, 1006, 1068, 1082, 1140
Hpy99I CGWCG 2 cut(s) 212, 215
HpyAV CCTTC 3 cut(s) 58, 928, 1388
HpyCH4III ACNGT 2 cut(s) 215, 625
HpyCH4IV ACGT 2 cut(s) 579, 586
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 863, 1385
HpyF3I CTNAG 3 cut(s) 525, 1075, 1243
HpySE526I ACGT 2 cut(s) 579, 586
Hsp92II CATG 7 cut(s) 85, 103, 162, 421, 865, 1201, 1213
HspAI GCGC 1 cut(s) 415
Kpn2I TCCGGA 1 cut(s) 1115
Ksp22I TGATCA 1 cut(s) 735
Kzo9I GATC 6 cut(s) 178, 294, 420, 495, 735, 922
LmnI GCTCC 5 cut(s) 96, 715, 721, 1156, 1351
Lsp1109I GCAGC 5 cut(s) 256, 654, 869, 1002, 1421
LweI GCATC 5 cut(s) 18, 171, 256, 1042, 1375
MabI ACCWGGT 1 cut(s) 123
MaeI CTAG 4 cut(s) 219, 636, 906, 1163
MaeII ACGT 2 cut(s) 579, 586
MaeIII GTNAC 2 cut(s) 77, 572
MalI GATC 6 cut(s) 180, 296, 422, 497, 737, 924
MbiI CCGCTC 1 cut(s) 1159
MboI GATC 6 cut(s) 178, 294, 420, 495, 735, 922
MfeI CAATTG 2 cut(s) 926, 1405
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 3 cut(s) 454, 687, 1322
MluCI AATT 6 cut(s) 378, 592, 926, 1405, 1500, 1510
MlyI GAGTC 4 cut(s) 340, 494, 550, 1419
MmeI TCCRAC 1 cut(s) 644
Mph1103I ATGCAT 1 cut(s) 164
MroI TCCGGA 1 cut(s) 1115
MseI TTAA 2 cut(s) 540, 582
MslI CAYNNNNRTG 3 cut(s) 38, 86, 1208
MspI CCGG 6 cut(s) 454, 657, 719, 831, 1009, 1116
MspR9I CCNGG 5 cut(s) 125, 454, 831, 1010, 1185
MunI CAATTG 2 cut(s) 926, 1405
Mva1269I GAATGC 2 cut(s) 252, 1263
MvaI CCWGG 2 cut(s) 125, 1185
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 3 cut(s) 71, 863, 1385
NciI CCSGG 3 cut(s) 454, 831, 1010
NcoI CCATGG 1 cut(s) 861
NdeII GATC 6 cut(s) 178, 294, 420, 495, 735, 922
NlaIII CATG 7 cut(s) 85, 103, 162, 421, 865, 1201, 1213
NlaIV GGNNCC 2 cut(s) 180, 717
NmeAIII GCCGAG 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 164
NspI RCATGY 3 cut(s) 85, 1201, 1213
NspV TTCGAA 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 1427
PceI AGGCCT 2 cut(s) 938, 1183
PciI ACATGT 2 cut(s) 81, 1209
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 2 cut(s) 252, 1263
PfeI GAWTC 6 cut(s) 13, 650, 955, 998, 1013, 1058
PflFI GACNNNGTC 1 cut(s) 623
PflMI CCANNNNNTGG 1 cut(s) 175
PkrI GCNGC 6 cut(s) 271, 644, 859, 992, 1161, 1436
PleI GAGTC 4 cut(s) 340, 494, 550, 1419
PpsI GAGTC 4 cut(s) 340, 494, 550, 1419
PscI ACATGT 2 cut(s) 81, 1209
Psp1406I AACGTT 1 cut(s) 579
Psp6I CCWGG 2 cut(s) 123, 1183
PspGI CCWGG 2 cut(s) 123, 1183
PspN4I GGNNCC 2 cut(s) 180, 717
PspPI GGNCC 4 cut(s) 127, 625, 676, 824
PstI CTGCAG 1 cut(s) 1339
PsuI RGATCY 1 cut(s) 178
PsyI GACNNNGTC 1 cut(s) 623
RsaI GTAC 2 cut(s) 506, 835
RsaNI GTAC 2 cut(s) 505, 834
RseI CAYNNNNRTG 3 cut(s) 38, 86, 1208
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 2 cut(s) 540, 582
SatI GCNGC 6 cut(s) 270, 643, 858, 991, 1160, 1435
Sau3AI GATC 6 cut(s) 178, 294, 420, 495, 735, 922
Sau96I GGNCC 4 cut(s) 127, 625, 676, 824
SchI GAGTC 4 cut(s) 340, 494, 550, 1419
ScrFI CCNGG 5 cut(s) 125, 454, 831, 1010, 1185
SduI GDGCHC 3 cut(s) 454, 687, 1322
SexAI ACCWGGT 1 cut(s) 123
SfaNI GCATC 5 cut(s) 18, 171, 256, 1042, 1375
SfcI CTRYAG 1 cut(s) 1335
Sfr274I CTCGAG 1 cut(s) 1427
SfuI TTCGAA 1 cut(s) 393
SgrDI CGTCGACG 1 cut(s) 209
SinI GGWCC 4 cut(s) 127, 625, 676, 824
SlaI CTCGAG 1 cut(s) 1427
SmiMI CAYNNNNRTG 3 cut(s) 38, 86, 1208
SmlI CTYRAG 4 cut(s) 531, 680, 1412, 1427
SmoI CTYRAG 4 cut(s) 531, 680, 1412, 1427
SpeI ACTAGT 1 cut(s) 905
Sse9I AATT 6 cut(s) 378, 592, 926, 1405, 1500, 1510
SseBI AGGCCT 2 cut(s) 938, 1183
SsiI CCGC 1 cut(s) 1159
SspMI CTAG 4 cut(s) 219, 636, 906, 1163
StuI AGGCCT 2 cut(s) 938, 1183
StyD4I CCNGG 5 cut(s) 123, 452, 829, 1008, 1183
StyI CCWWGG 4 cut(s) 19, 861, 939, 1258
TaaI ACNGT 2 cut(s) 215, 625
TaiI ACGT 2 cut(s) 582, 589
TaqI TCGA 9 cut(s) 26, 210, 384, 393, 498, 699, 1095, 1189, 1428
TasI AATT 6 cut(s) 378, 592, 926, 1405, 1500, 1510
TauI GCSGC 1 cut(s) 1162
TfiI GAWTC 6 cut(s) 13, 650, 955, 998, 1013, 1058
Tru1I TTAA 2 cut(s) 540, 582
Tru9I TTAA 2 cut(s) 540, 582
TseI GCWGC 5 cut(s) 269, 642, 857, 990, 1434
TspDTI ATGAA 1 cut(s) 103
TspGWI ACGGA 1 cut(s) 252
Tth111I GACNNNGTC 1 cut(s) 623
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 4 cut(s) 127, 625, 676, 824
XapI RAATTY 1 cut(s) 1500
XceI RCATGY 3 cut(s) 85, 1201, 1213
XhoI CTCGAG 1 cut(s) 1427
XmiI GTMKAC 3 cut(s) 210, 947, 1081
XspI CTAG 4 cut(s) 219, 636, 906, 1163
Zsp2I ATGCAT 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.