Rw2G010300

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003681
Physical Location & Seq
Reverse (-)
10390759 .. 10391691
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010300.1

Sequence Viewer

Length: 894 bp
ATGGAGGGGCAATATTGTGATGACCACAGCAATTTCAATTTCAAAGGTATGATGCCCACAGCTATATCCTCCTTTTTCTCGGCCTATGCCCCCTTTGCTGGTTACATGTTTATGCTCAAATCCATGAACACCAATTTCCTCCCTTACGACCTCAGCTACCTCCTGGACCCTCTAGCCAATCACATAACTCTCCTCATCGACAACTCTAGGGATCACTGCTCGGACGAAATTTCGCAGCGCAACCTAGTCTATGATGCTGCTGAGGTCTATCTTGCCACCAAGATCAACCCGAAAACCCAAATCTTTCGAATCAGCAAAGCACCGAAACAAAAGGTCCCCATCATTGTTGTTACCACCACCCAAGAAATCGCCGACACTTTCGAAAACATTAAAGTAAAGTGGCGCATGATCTTCCCTGAAGAAAAAGACCGTCACAGTCACTACAGTAGGCTCAATAAGAGCCGCTTTGAGCTAACATTCCACAGGAAACACAAAGACAAAGTTATAAACTCGTACATCCCCTATGTATTGGCTCGGGCCGAGGCTATCAAAGATGCAGAAAAGACTCTAAAGCTTTATAGTTACAATAGTAAACGTTCCTCGGCCCATTACTTTAAACCTTCTGGTGGACGCTCTCAAGGTAGTGATGACGATGATGACTTTGCACCTTCTTCAGGCAATTGGAGTTGCGCGGATAATGAGCACCCAGCTACTTTCGAGACAATGTCGGAGCTGAAGAGAATTATCATAGATGATTTGGACAAGTTCGTGAAGAGGAGGGAGTTCTACAAGAGACTGAGAGAGTTGGGAAGGCTTGGAAAGTTGGAAGAGAGGCTACTTGTTGTTTGGTCCACCCGGCACTGGAAAATCCAGCTTGGTAGCGGCCATGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

297

Amino Acids

34.55

Weight (kDa)

9.0

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 53 - 136 5.9e-13 Domain associated at C-terminal with AAA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 506
AccII CGCG 1 cut(s) 692
AciI CCGC 3 cut(s) 463, 692, 882
AclI AACGTT 1 cut(s) 595
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 883
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 3 cut(s) 438, 657, 755
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 4 cut(s) 98, 626, 674, 861
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 2 cut(s) 37, 43
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 7 cut(s) 62, 156, 472, 574, 710, 733, 874
AluI AGCT 7 cut(s) 62, 156, 472, 574, 710, 733, 874
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 2 cut(s) 713, 787
AlwI GGATC 1 cut(s) 219
Ama87I CYCGRG 1 cut(s) 534
AoxI GGCC 4 cut(s) 81, 537, 603, 883
ApeKI GCWGC 2 cut(s) 235, 257
ApoI RAATTY 1 cut(s) 228
AspLEI GCGC 3 cut(s) 240, 405, 692
AspS9I GGNCC 5 cut(s) 166, 334, 537, 604, 849
AsuC2I CCSGG 1 cut(s) 856
AsuII TTCGAA 2 cut(s) 307, 381
AvaI CYCGRG 1 cut(s) 534
AvaII GGWCC 3 cut(s) 166, 334, 849
BarI GAAGNNNNNNTAC 2 cut(s) 819, 851
Bbv12I GWGCWC 1 cut(s) 705
BbvCI CCTCAGC 2 cut(s) 152, 261
BbvI GCAGC 2 cut(s) 244, 247
BccI CCATC 1 cut(s) 347
BciT130I CCWGG 1 cut(s) 164
BcnI CCSGG 1 cut(s) 856
BcoDI GTCTC 2 cut(s) 713, 787
BfaI CTAG 3 cut(s) 173, 207, 245
BfmI CTRYAG 1 cut(s) 442
BisI GCNGC 4 cut(s) 236, 258, 463, 883
BlsI GCNGC 4 cut(s) 237, 259, 464, 884
Bme1390I CCNGG 2 cut(s) 164, 856
Bme18I GGWCC 3 cut(s) 166, 334, 849
BmeT110I CYCGRG 1 cut(s) 534
BmgT120I GGNCC 5 cut(s) 166, 334, 537, 604, 849
BmiI GGNNCC 2 cut(s) 168, 336
BmrFI CCNGG 2 cut(s) 164, 856
BmsI GCATC 3 cut(s) 42, 244, 544
Bpu10I CCTNAGC 2 cut(s) 152, 261
Bpu14I TTCGAA 2 cut(s) 307, 381
BpuEI CTTGAG 1 cut(s) 621
BpuMI CCSGG 1 cut(s) 856
BsaJI CCNNGG 3 cut(s) 540, 600, 886
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 626, 674, 861
Bse1I ACTGG 1 cut(s) 866
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 3 cut(s) 540, 600, 886
BseGI GGATG 1 cut(s) 516
BseLI CCNNNNNNNGG 4 cut(s) 98, 626, 674, 861
BseMII CTCAG 3 cut(s) 166, 252, 788
BseNI ACTGG 1 cut(s) 866
BseRI GAGGAG 2 cut(s) 182, 790
BseXI GCAGC 2 cut(s) 244, 247
BseYI CCCAGC 1 cut(s) 706
Bsh1236I CGCG 1 cut(s) 692
BshFI GGCC 4 cut(s) 83, 539, 605, 885
BsiHKAI GWGCWC 1 cut(s) 705
BsiHKCI CYCGRG 1 cut(s) 534
BsiSI CCGG 1 cut(s) 856
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 4 cut(s) 98, 626, 674, 861
BsmAI GTCTC 2 cut(s) 713, 787
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 4 cut(s) 83, 539, 605, 885
BsoBI CYCGRG 1 cut(s) 534
Bsp119I TTCGAA 2 cut(s) 307, 381
Bsp1286I GDGCHC 1 cut(s) 705
Bsp143I GATC 3 cut(s) 211, 282, 408
Bsp19I CCATGG 1 cut(s) 886
BspACI CCGC 3 cut(s) 463, 692, 882
BspANI GGCC 4 cut(s) 83, 539, 605, 885
BspCNI CTCAG 3 cut(s) 165, 253, 789
BspFNI CGCG 1 cut(s) 692
BspLI GGNNCC 2 cut(s) 168, 336
BspPI GGATC 1 cut(s) 219
BspT104I TTCGAA 2 cut(s) 307, 381
BsrI ACTGG 1 cut(s) 866
BssECI CCNNGG 3 cut(s) 540, 600, 886
BssMI GATC 3 cut(s) 211, 282, 408
BssT1I CCWWGG 1 cut(s) 886
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 3 cut(s) 431, 437, 446
Bst6I CTCTTC 3 cut(s) 731, 767, 822
BstBI TTCGAA 2 cut(s) 307, 381
BstDEI CTNAG 3 cut(s) 152, 261, 797
BstDSI CCRYGG 1 cut(s) 886
BstENI CCTNNNNNAGG 1 cut(s) 672
BstF5I GGATG 1 cut(s) 516
BstFNI CGCG 1 cut(s) 692
BstHHI GCGC 3 cut(s) 240, 405, 692
BstKTI GATC 3 cut(s) 214, 285, 411
BstMAI GTCTC 2 cut(s) 713, 787
BstMBI GATC 3 cut(s) 211, 282, 408
BstMWI GCNNNNNNNGC 2 cut(s) 95, 888
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 2 cut(s) 162, 854
BstSFI CTRYAG 1 cut(s) 442
BstUI CGCG 1 cut(s) 692
BstV1I GCAGC 2 cut(s) 244, 247
BsuRI GGCC 4 cut(s) 83, 539, 605, 885
BtgI CCRYGG 1 cut(s) 886
BtsCI GGATG 1 cut(s) 516
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 214, 859
CfoI GCGC 3 cut(s) 240, 405, 692
Cfr13I GGNCC 5 cut(s) 166, 334, 537, 604, 849
CseI GACGC 1 cut(s) 639
Csp6I GTAC 1 cut(s) 514
CviAII CATG 4 cut(s) 106, 124, 406, 887
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 3 cut(s) 152, 261, 797
DpnI GATC 3 cut(s) 213, 284, 410
DpnII GATC 3 cut(s) 211, 282, 408
DraI TTTAAA 1 cut(s) 616
EaeI YGGCCR 1 cut(s) 883
Eam1104I CTCTTC 3 cut(s) 731, 767, 822
EarI CTCTTC 3 cut(s) 731, 767, 822
Eco130I CCWWGG 1 cut(s) 886
Eco47I GGWCC 3 cut(s) 166, 334, 849
Eco57I CTGAAG 3 cut(s) 438, 657, 755
Eco88I CYCGRG 1 cut(s) 534
EcoNI CCTNNNNNAGG 1 cut(s) 672
EcoO109I RGGNCCY 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 886
ErhI CCWWGG 1 cut(s) 886
FaeI CATG 4 cut(s) 109, 127, 409, 890
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FaqI GGGAC 1 cut(s) 320
FatI CATG 4 cut(s) 105, 123, 405, 886
Fnu4HI GCNGC 4 cut(s) 236, 258, 463, 883
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 4 cut(s) 236, 258, 463, 883
FspBI CTAG 3 cut(s) 173, 207, 245
GlaI GCGC 3 cut(s) 239, 404, 691
GluI GCNGC 4 cut(s) 236, 258, 463, 883
GsaI CCCAGC 1 cut(s) 710
HaeIII GGCC 4 cut(s) 83, 539, 605, 885
HapII CCGG 1 cut(s) 856
HgaI GACGC 1 cut(s) 639
HhaI GCGC 3 cut(s) 240, 405, 692
Hin1II CATG 4 cut(s) 109, 127, 409, 890
Hin6I GCGC 3 cut(s) 238, 403, 690
HinP1I GCGC 3 cut(s) 238, 403, 690
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 2 cut(s) 309, 565
HpaII CCGG 1 cut(s) 856
Hpy166II GTNNAC 3 cut(s) 593, 629, 852
Hpy188I TCNGA 2 cut(s) 223, 730
Hpy188III TCNNGA 2 cut(s) 718, 769
Hpy8I GTNNAC 3 cut(s) 593, 629, 852
HpyAV CCTTC 3 cut(s) 630, 678, 804
HpyCH4III ACNGT 3 cut(s) 431, 437, 446
HpyCH4IV ACGT 1 cut(s) 595
HpyCH4V TGCA 2 cut(s) 557, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 888
HpyF3I CTNAG 3 cut(s) 152, 261, 797
HpySE526I ACGT 1 cut(s) 595
Hsp92II CATG 4 cut(s) 109, 127, 409, 890
HspAI GCGC 3 cut(s) 238, 403, 690
Kzo9I GATC 3 cut(s) 211, 282, 408
LmnI GCTCC 1 cut(s) 730
Lsp1109I GCAGC 2 cut(s) 244, 247
LweI GCATC 3 cut(s) 42, 244, 544
MaeI CTAG 3 cut(s) 173, 207, 245
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 5 cut(s) 101, 349, 431, 437, 581
MalI GATC 3 cut(s) 213, 284, 410
MboI GATC 3 cut(s) 211, 282, 408
MboII GAAGA 6 cut(s) 403, 431, 663, 748, 784, 839
MfeI CAATTG 1 cut(s) 679
MhlI GDGCHC 1 cut(s) 705
MluCI AATT 6 cut(s) 31, 37, 133, 228, 679, 741
MlyI GAGTC 1 cut(s) 559
MmeI TCCRAC 2 cut(s) 708, 804
MseI TTAA 2 cut(s) 390, 615
MslI CAYNNNNRTG 1 cut(s) 110
MspI CCGG 1 cut(s) 856
MspR9I CCNGG 2 cut(s) 164, 856
MunI CAATTG 1 cut(s) 679
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 1 cut(s) 692
MwoI GCNNNNNNNGC 2 cut(s) 95, 888
NciI CCSGG 1 cut(s) 856
NcoI CCATGG 1 cut(s) 886
NdeII GATC 3 cut(s) 211, 282, 408
NlaIII CATG 4 cut(s) 109, 127, 409, 890
NlaIV GGNNCC 2 cut(s) 168, 336
NmeAIII GCCGAG 3 cut(s) 59, 565, 581
NmuCI GTSAC 2 cut(s) 431, 437
NspI RCATGY 1 cut(s) 109
NspV TTCGAA 2 cut(s) 307, 381
PciI ACATGT 1 cut(s) 105
PfeI GAWTC 1 cut(s) 309
PflFI GACNNNGTC 1 cut(s) 724
PfoI TCCNGGA 1 cut(s) 162
PkrI GCNGC 4 cut(s) 237, 259, 464, 884
PleI GAGTC 1 cut(s) 559
PpsI GAGTC 1 cut(s) 559
PpuMI RGGWCCY 1 cut(s) 334
PscI ACATGT 1 cut(s) 105
PsiI TTATAA 1 cut(s) 506
Psp1406I AACGTT 1 cut(s) 595
Psp5II RGGWCCY 1 cut(s) 334
Psp6I CCWGG 1 cut(s) 162
PspFI CCCAGC 1 cut(s) 706
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 168, 336
PspPI GGNCC 5 cut(s) 166, 334, 537, 604, 849
PspPPI RGGWCCY 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 724
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
RseI CAYNNNNRTG 1 cut(s) 110
SaqAI TTAA 2 cut(s) 390, 615
SatI GCNGC 4 cut(s) 236, 258, 463, 883
Sau3AI GATC 3 cut(s) 211, 282, 408
Sau96I GGNCC 5 cut(s) 166, 334, 537, 604, 849
SchI GAGTC 1 cut(s) 559
ScrFI CCNGG 2 cut(s) 164, 856
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 3 cut(s) 42, 244, 544
SfcI CTRYAG 1 cut(s) 442
SfuI TTCGAA 2 cut(s) 307, 381
SinI GGWCC 3 cut(s) 166, 334, 849
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 636
SmoI CTYRAG 1 cut(s) 636
Sse9I AATT 6 cut(s) 31, 37, 133, 228, 679, 741
SsiI CCGC 3 cut(s) 463, 692, 882
SspI AATATT 1 cut(s) 14
SspMI CTAG 3 cut(s) 173, 207, 245
StyD4I CCNGG 2 cut(s) 162, 854
StyI CCWWGG 1 cut(s) 886
TaaI ACNGT 3 cut(s) 431, 437, 446
TaiI ACGT 1 cut(s) 598
TaqI TCGA 4 cut(s) 198, 307, 381, 717
TasI AATT 6 cut(s) 31, 37, 133, 228, 679, 741
TauI GCSGC 2 cut(s) 465, 885
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 2 cut(s) 390, 615
Tru9I TTAA 2 cut(s) 390, 615
TscAI CASTG 2 cut(s) 221, 866
TseFI GTSAC 2 cut(s) 431, 437
TseI GCWGC 2 cut(s) 235, 257
Tsp45I GTSAC 2 cut(s) 431, 437
TspDTI ATGAA 1 cut(s) 140
TspRI CASTG 2 cut(s) 221, 866
Tth111I GACNNNGTC 1 cut(s) 724
VpaK11BI GGWCC 3 cut(s) 166, 334, 849
XagI CCTNNNNNAGG 1 cut(s) 672
XapI RAATTY 1 cut(s) 228
XceI RCATGY 1 cut(s) 109
XspI CTAG 3 cut(s) 173, 207, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.